{"product_id":"caspase-9-activity-assay-kit-colorimetric-method","title":"Caspase 9 Activity Assay Kit(Colorimetric Method)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:100Assays\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e:This product can be stored at -20°C for 12 months with shading light.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShipping\u003c\/b\u003e:Gel Packs\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExp date\u003c\/b\u003e:12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_I\u003c\/b\u003e:Apoptosis and Cell Health Detection\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_II\u003c\/b\u003e:Cell Apoptosis\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_III\u003c\/b\u003e:Caspase Assay Kits\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecies reactivity\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUsage\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eKit Type\u003c\/b\u003e:Apoptosis\/Caspase\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResult Type\u003c\/b\u003e:Qualitative or quantitative\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDetection Instrument\u003c\/b\u003e:Spectrophotometer;Microplate reader\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTested Sample Types\u003c\/b\u003e:Cell and tissue samples\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay Time\u003c\/b\u003e:4-6 hours\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDetection Type\u003c\/b\u003e:Colorimetric method\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTest Principle\u003c\/b\u003e:Caspase 9, also known as ICE-LAP6 or Mch6, is a relatively upstream caspase in the process of apoptosis signal transduction. After mitochondria release cytochrome c, caspase 9 can form complex with cytochrome c and Apaf1 and can be activated. Activated caspase 9 can activate caspase 3, the most critical enzyme of apoptosis, thereby promoting subsequent apoptotic signals. The activation of caspase 9 can be regulated by phosphorylation. This caspase 9 activity assay kit is based on caspase 9 that can catalyze the substrate Ac-LEHD-pNA to generate yellow pNA (p-Nitroaniline). The pNA has a strong absorption near 405 nm, The activity of caspase 9 can be calculated by determining the absorbance at 405 nm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBinding Capacity\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBuffer\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated MW\u003c\/b\u003e:\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48665605570660,"sku":"E-CK-A389","price":478.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_0321bc2b-46e1-448d-9c7c-caa8e4d3f71e.jpg?v=1782866818","url":"https:\/\/www.genebiosystems.com\/products\/caspase-9-activity-assay-kit-colorimetric-method","provider":"GeneBio ","version":"1.0","type":"link"}