{"product_id":"camp-elisa-kit","title":"cAMP ELISA KIT","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:96T\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplications\u003c\/b\u003e:ELISA\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStability and Storage\u003c\/b\u003e:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 10% prior to the expiration date under appropriate storage condition.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDetection method\u003c\/b\u003e:Colorimetric\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSample type\u003c\/b\u003e:Plasma, Serum\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay type\u003c\/b\u003e:Quantitative\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSensitivity\u003c\/b\u003e:1.56 pmol\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRange\u003c\/b\u003e:1.56-200 pmol\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRecovery\u003c\/b\u003e:80-120%\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExperimental Procedure\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003eAdenosine 3', 5'-cyclic monophosphate (cAMP) is a key second messenger molecule in intracellular signal transduction. Monitoring cAMP levels is one of the most common ways to screen for agonists and antagonists of G protein–coupled receptors(GPCRs). Bioyeargene cAMP ELISA Kit is based on the competition between HRP-labeled cAMP and free cAMP for fixed amounts of mouse monoclonal anti-cAMP antibody(cAMP MAb) binding sites. Because the concentration of the HRP-labeled cAMP is held constant while the concentration of cAMP varies, the amount of HRP-labeled cAMP that is able to bind to the cAMP MAb will be inversely proportional to the concentration of cAMP in the well. This antigen-antibody complex binds to the goat anti-mouse IgG that has been previously attached to the well. The plate is washed to remove any unbound reagents and then developed. The ELISA typically displays an IC50(50% B\/B0) of approximately 12 pmol\/mL and a detection limit of approximately 0.15 pmol\/mL.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eAlternative Names\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShipping\u003c\/b\u003e:2-8 °C\u003c\/p\u003e\u003cp\u003e\u003cb\u003eNote\u003c\/b\u003e:For research use only.\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48656109273188,"sku":"YP051018","price":1451.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_bb30b5d8-9fb1-49b0-9426-b4c4efbcacee.jpg?v=1782809259","url":"https:\/\/www.genebiosystems.com\/products\/camp-elisa-kit","provider":"GeneBio ","version":"1.0","type":"link"}