{"product_id":"ack-phospho-tyr284-rabbit-pab-antibody","title":"ACK (phospho Tyr284) rabbit pAb","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 100μL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSource\u003c\/b\u003e:Rabbit\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplications\u003c\/b\u003e:WB;IHC;IF;ELISA\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e:Human;Mouse\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDilution\u003c\/b\u003e:Western Blot: 1\/500 - 1\/2000. Immunohistochemistry: 1\/100 - 1\/300. Immunofluorescence: 1\/200 - 1\/1000. ELISA: 1\/10000. Not yet tested in other applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eImmunogen\u003c\/b\u003e:The antiserum was produced against synthesized peptide derived from human ACK1 around the phosphorylation site of Tyr284. AA range:250-299\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage_stability\u003c\/b\u003e:-20°C\/1 year\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality\u003c\/b\u003e:Polyclonal\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e:IgG\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e:1 mg\/ml\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObserved_band(KD)\u003c\/b\u003e:120kD\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHuman_gene_id\u003c\/b\u003e:10188\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHuman_swiss_prot_no\u003c\/b\u003e:Q07912\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSubcellular_location\u003c\/b\u003e:Cell membrane . Nucleus . Endosome . Cell junction, adherens junction . Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side . Cytoplasmic vesicle, clathrin-coated vesicle . Membrane, clathrin-coated pit . Cytoplasm, perinuclear region . Cytoplasm, cytosol . The Tyr-284 phosphorylated form is found both in the membrane and nucleus (By similarity). Co-localizes with EGFR on endosomes (PubMed:20333297). Nuclear translocation is CDC42-dependent (By similarity). Detected in long filamentous cytosolic structures where it co-localizes with CTPS1 (By similarity). .\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOther_name\u003c\/b\u003e:TNK2; ACK1; Activated CDC42 kinase 1; ACK-1; Tyrosine kinase non-receptor protein 2\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e:This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Jul 2008],\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48647198867556,"sku":"ES4434","price":477.4,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_979e6822-5d89-4e86-8a7d-2adf5bb832ca.jpg?v=1782691782","url":"https:\/\/www.genebiosystems.com\/products\/ack-phospho-tyr284-rabbit-pab-antibody","provider":"GeneBio ","version":"1.0","type":"link"}