TransStart® Green qPCR SuperMix UDG

Description

TransStart® Green qPCR SuperMix UDG is a ready-to-use qPCR cocktail containing all components, except primer and template. It contains TransStart® Taq DNA Polymerase, UDG, SYBR Green I, dNTPs, PCR enhancer and stabilizer. qPCR SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primer, passive reference dye (optional) and ddH2O.

Highlights

• TransStart® Taq DNA Polymerase, hot start with double blocking technique, improves sensitivity, enhances specificity and generates more accurate data.
  • Double cation (K+, NH4+) buffer enhances specificity and reduces primer-dimer formation.
  • Passive reference dyes are provided for different qPCR instruments.
  • UDG and dUTP avoid cross contamination.

Passive Reference

Dye Selection

• Passive Reference Dye I (50×)

ABI Prism7000/7300/7700/7900, Eppendorf, ABI Step One, ABI Step One Plus
• Passive Reference Dye II (50×)
ABI Prism7500, ABI Prism7500 Fast, ABI Q6, ABI Quant Studio 6/7 Flex, ABI ViiA 7, Stratagene Mx3000/Mx3005P, Corbett Rotor Gene 3000
• No Passive Reference Dye
Roche LightCycler480, Roche Light Cycler96, MJ Research Chromo4, Opticon (II), Takara TP800, Bio-Rad iCycler iQ, iQ5, Bio-Rad CFX96, Bio-Rad C1000 Thermal Cycler, Thermo Pikoreal 96, Corbett Rotor Gene 6000, Corbett Rotor Gene G, Corbett Rotor Gene Q

Storage

at -20℃ for one year

Kit Contents

Component

AQ111-01

AQ111-02

AQ111-03

TransStart® Green qPCR SuperMix UDG (2×)

1 ml

5×1 ml

15×1 ml

Passive Reference Dye (50×)

40 μl

200 μl

600 μl

ddH2O

1 ml

5 ml

3×5 ml