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GB-Amp™ Taq DNA Polymerase

P101

Size: 1000 U

High purity recombinant Taq DNA polymerase. For your routine PCR, for amplifying 4 kb or smaller fragments with GC content below 60%.

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CAD$55.00

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Other sizes of this product are also available. Please inquire. 

Description

GB-Amp™ Taq DNA Polymerase is purified from E. coli. expressing a cloned Thermus aquaticus DNA polymerase gene. This enzyme has a 5' → 3' DNA polymerase and a 5' → 3' exonuclease activity but lacks a 3' → 5' exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. Taq DNA polymerase is heat-stable.

Contents

Taq DNA Polymerase by default is packaged in 5 U/μl.

10×PCR Buffer, Mg++, 6 x gel loading buffer are also included


Applications
Standard PCR
DNA labeling
DNA sequencing
Numerous applications for which a high-quality, thermostable DNA polymerase is required

Unit Definition
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.

Storage Buffer

20mM TrisCl ( pH8.0), 100mM KCl, 3.2mM MgCl2 1mM DTT,0.1% Triton X-100 ,0.1% Tween20, 0.2mg/ml BSA, 50% (v/v) glycerol

Note

-Recombinant Taq DNA Polymerase is the enzyme of choice for most PCR applications.

-The half-life of enzyme is >40 minutes at 95°C.

-The error rate of Taq DNA Polymerase in PCR is 2.2x10-5 errors per nt per cycle;

the accuracy (an inverse of the error rate) an average number of correct nucleotides incorporated before making an error, is 4.5x104.

- Taq DNA Polymerase accepts modified nucleotides (e.g. biotin-, digoxigenin-, fluorescent-labeled nucleotides) as substrates for the DNA synthesis.Compatible with TA cloning – generates PCR products with 3’-dA overhangs.

-Suitable for Taqman probe real time PCR

-Recommendations with Template DNA in a 50μl reaction volume

-recommended quantities of template per react

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Store all components at –20°C

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