PCR Plates-96 well Plates

Sizes: 25 plates or 100 plates

8 different designs of PCR plates, choice of:

a) Low Profile (lower height, smaller volume wells) vs. Standard Profile (higher, larger volume wells);

b) White plates vs. transparent (clear) plates

c) Skirted Plates vs. Non-skirted Plates; 

Please select your options when placing your order. Ask us if you need help. 

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CAD$50.00

  • 25 plates
  • 100 plates

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Manufactured in our ISO9000 certified cleanroom, DNase/RNase-free, autoclavable at 121 C and freezable t -80 C.

Please Select one of the products below and indicate your choice on the order:

White Plate or Clear Plate?

Standard Profile vs. Low Profile?

Skirted Plate vs. Non-skirted Plate?

Recommended Product

(catalogue number)

White

Standard

Skirted

PCR-FS96WSP

White

Standard

Non-skirted

PCR-NH96WSP

White

Low

Skirted

PCR-FS96WLP

White

Low

Non-skirted

PCR-NH96WLP

Clear

Standard

Skirted

PCR-FS96SP

Clear

Standard

Non-skirted

PCR-NH96SP

Clear

Low

Skirted

PCR-FS96LP

Clear

Low

Non-skirted

PCR-NH96LP

See below for our PCR Plate Selection Guide, or  Ask us if you need help.  

SELECTION GUIDE: 96-WELL PCR PLATES

White Vs. Clear:

White plates and tubes direct significantly more signal back to the qPCR instrument's detector than traditional clear plates. The improved signal reflection ensures that even the lowest levels of fluorescence are detected. White well walls also stop signal from passing through to the cycler block where it can be inconsistently reflected or absorbed, leading to increase noise levels in detected fluorescence. This keeps variations in the cycler block from affecting qPCR data. Our white PCR plastics increases sensitivity, reduces variability and increases the signal-to-noise ratio within the qPCR assay.

Clear plates provide peace of mind in being able to verify pipetting steps right after each pipetting action, as you can see the reagent added into the well as you intended. Clear plates are suitable for most applications.

These considerations also apply to 8-strip wells and individual PCR tubes.

 

Standard Profile vs. Low Profile

Standard-profile (full-height; around 21.0 mm) wells are “standard” relative to the low-profile plates and fit in most classical thermal cyclers and real-time PCR detection systems, such as Bio-Rad's iCycler®, iQ™ series real-time PCR detection systems, and Applied Biosystems' regular PCR systems and DNA sequencers. These plates let you use large reaction volumes.  These plates reduce risk-splashing caused cross-over contamination, minimizing the risk of well-to-well cross contamination.

Low-profile (around 15.5 mm) wells are the newer design that reduces the potential for condensate formation and offers advantages for light capture in fluorescence assays, low-volume reactions, and fast PCR. They have been recommended for newer instruments such as Bio-Rad's C1000 Touch™ and S1000™ thermal cyclers, CFX series real-time PCR detection systems, and Applied Biosystems' fast PCR systems. Low profile plates reduce dead space and increase PCR efficiency.

Please consult the manual of the PCR system, if necessary or ask us. Instruments with adjustable lids can usually use both standard-profile and low-profile wells. These instruments include Bio-Rad's 1000-series and DNA Engine® series thermal cyclers. Some systems require standard profile or low profile plates only.

These considerations also apply to 8-strip wells and individual PCR tubes.

 

Skirted vs no skirted.

Skirted plates are more warp-resistant because the rigidity provided by the skirt on the outer perimeter of the plate.  These plates also allow for labeling or barcoding.

Non-skirted format compatible with most thermal cyclers. These plates can also be readily cut into sectional sizes as required by user, so that the entire plate is not wasted, when fewer than 96 wells are required for some experiments.

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