The 10bp DNA Ladder is ideal for determining size of double-stranded DNA from 80 to 300 base pairs in fine details. The ladder consists of 18 double-stranded fragments. The 100 and 200 bp fragments are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. This ladder is pre-mixed with loading dye and ready to use.
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The 10bp DNA Ladder is ideal for determining the size of double-stranded DNA from 80 to 300 base pairs. The ladder consists of 18 linear double-stranded fragments. The 100bp and 200bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 µl loading, all fragments except 100bp and 200bp are 40ng. The 100bp and 200bp fragment are 100ng. This ladder is pre-mixed with loading dye and is ready to use.
Recommended Loading
2-5 µl/Lane
Concentration
Typical Bands 100 ng/5µl
Other Bands 40 ng/5µl
Advised Electrophoresis Condition
1 µl/Lane, 20 cm ,10%Polyacrylamide Gel, 1×TBE, 8 V/cm, 3h.
Contents(bp)
80、90、100、110、120、130、140、150、160、170、180、190、200、220、240、260、280、300
Mix Concentration
168 ng/µl
Storage
Stable for 3 months at RT, for long-time storage, please store at -20℃.