GB-View™ nucleic acid stain is a high-sensitivity dye for visualizing double-stranded DNA, single-stranded DNA, and RNA in agarose gels. Non-carcinogenic by the Ames-test, it is developed to replace toxic Ethidium Bromide (a potent mutagen), commonly used in gel electrophoresis for visualization of nucleic acids in agarose gels.
Works for both UV and blue light.
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1) Unlike other dyes, GB-View does not alter the mobility of DNA on gel, so you can determine your the size of your DNA accurately!
2) This DNA Gel electrophoresis dye works for UV transilliminators and Blue LED transilluminators, and UV and LED based gel doc systems. Yes, including your Bio-Rad Imaging systems.
3) GB-View is a safer dye than is Ethidium Bromide.
It emits green fluorescence with broad spectral wavelength range. This new stain has two fluorescence excitation maximas when bound to nucleic acid, one centered at 268 nm and another at 294 nm. In addition, it has one visible excitation at 491 nm. This is why this dye has the dual compatibility with transilluminators/gel doc systems based on UV or Blue Light excitation.
The Fluorescence emission of GB-View™ bound to DNA is centered at 530 nm. These spectral characteristics make GB-View™ I compatible with a wide variety of gel reading instruments, ranging from those with ultraviolet epi- and transillumination to argon-ion laser and mercury-arc lamp excitation gel scanners.
GB-View™ products are non-carcinogenic by the Ames-test. The results are negative in both the mouse marrow chromophilous erythrocyte micronucleus and mous spermary spermatocyte chromosomal aberration tests. Although GB-View™ Nucleic Acid stains are non-carcinogenic, it may cause skin and eye-irritations. Always wear gloves when working with the product.
Protocol 1. Prepare 100 ml of agarose gel solution (concentration from 0.8~3%) in a 250 ml flask and mix it thoroughly. Place the flask in the microwave, heat it until the solution is completely clear and no small floating particles are visible (about 2~3 minutes).
2. Add 2-5 ul of GB-View™ to the gel solution. Swirl the flask gently to mix the solution and avoid forming bubbles.
3. While the gel solution cools, pour it into the gel tray until the comb teeth are immersed about 1/4~1/2 into the gel solution.
4. Allow the agarose gel to cool until solidified. Load samples on the gel and perform electrophoresis.
5. Detect the bands under UV illumination.
DNA Staining Sensitivity Test