{"product_id":"recombinant-mouse-glipr1-protein-his-tag","title":"Recombinant Mouse GLIPR1 Protein (His Tag)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 100μg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShipping\u003c\/b\u003e: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExp date\u003c\/b\u003e: 12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_II\u003c\/b\u003e: Recombinant Proteins\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_III\u003c\/b\u003e: Others\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Synonym\u003c\/b\u003e: 2410114O14Rik;RTVP-1;RTVP1;mRTVP-1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e: Mouse\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExpression Host\u003c\/b\u003e: HEK293 Cells\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eFusion tag\u003c\/b\u003e: C-His\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUNIProt ID\u003c\/b\u003e: Q4QQK5\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e: NP_082884.1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: Glioma pathogenesis-related protein  1, also known as Protein RTVP-1, GLIPR1 and GLIPR, is a single-pass membrane protein which belongs  to theCRISP family. GLIPR1 \/ RTVP-1 was expressed in high levels in  glioblastomas, whereas its expression in low-grade astrocytomas and normal  brains was very low. Transfection of glioma cells with small interfering RNAs  targeting GLIPR1 \/ RTVP-1 decreased cell proliferation in all the cell lines  examined and induced cell apoptosis in some of them. Overexpression of GLIPR1 \/  RTVP-1 increased astrocyte and glioma cell proliferation and the anchorage-independent  growth of the cells. In addition, overexpression of GLIPR1 \/ RTVP-1 rendered  glioma cells more resistant to the apoptotic effect of tumor necrosis  factor-related apoptosis-inducing ligand and serum deprivation. GLIPR1 \/ RTVP-1  regulated the invasion of glioma cells was evident by their enhanced migration  through Matrigel and by their increased invasion in a spheroid confrontation  assay. The increased invasive potential of the GLIPR1 \/ RTVP-1 overexpressors  was also shown by the increased activity of matrix metalloproteinase 2 in these  cells. The expression of GLIPR1 \/ RTVP-1 is correlated with the degree of  malignancy of astrocytic tumors and that GLIPR1 \/ RTVP-1 is involved in the  regulation of the growth, survival, and invasion of glioma cells. GLIPR1 \/  RTVP-1 is a potential therapeutic target in gliomas.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eActivity\u003c\/b\u003e: Not validated for activity\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e: Met1-Thr223\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:  \u0026gt; 85 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Lyophilized from sterile PBS, pH 7.4 \u003cbr\u003eNormally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.\u003cbr\u003ePlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e: Please refer to the printed manual for detailed information.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:  \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated MW\u003c\/b\u003e: 25.1 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObservedMW\u003c\/b\u003e: 28-32 kDa\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":47986023563364,"sku":"PKSM040455","price":1131.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_ebca4aed-1420-4356-924d-0da982a4bc62.jpg?v=1772479683","url":"https:\/\/www.genebiosystems.com\/en-us\/products\/recombinant-mouse-glipr1-protein-his-tag","provider":"GeneBio ","version":"1.0","type":"link"}