{"product_id":"recombinant-human-protein-disulfide-isomerase-proteinp4hb-active","title":"Recombinant Human Protein disulfide-isomerase protein(P4HB) (Active)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:100ug. Other sizes are also available. Please contact us. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e:Signal Transduction\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniprot NO.\u003c\/b\u003e:P07237\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniprot Entry Name\u003c\/b\u003e:PDIA1_HUMAN\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene Names\u003c\/b\u003e:P4HB,ERBA2L,PDI,PDIA1,PO4DB\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecies\u003c\/b\u003e:Homo sapiens (Human)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSource\u003c\/b\u003e:E.Coli\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExpression Region\u003c\/b\u003e:19-508aa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e:MRGSGSHHHHHH+\nAPEEEDHVLVLRKSNFAEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFRNGDTASPKEYTAGREADDIVNWLKKRTGPAATTLPDGAAAESLVESSEVAVIGFFKDVESDSAKQFLQAAEAIDDIPFGITSNSDVFSKYQLDKDGVVLFKKFDEGRNNFEGEVTKENLLDFIKHNQLPLVIEFTEQTAPKIFGGEIKTHILLFLPKSVSDYDGKLSNFKTAAESFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESEELTAERITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQDGAGDDDDLEDLEEAEEPDMEEDDDQKAVKDEL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProtein Description\u003c\/b\u003e:Partial\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTag Info\u003c\/b\u003e:N-terminal 6xHis-tagged\u003c\/p\u003e\u003cp\u003e\u003cb\u003eMol. Weight\u003c\/b\u003e:56.6 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBiological_Activity\u003c\/b\u003e:Thiol Protein Reductase Activity is 0.001 ?650nm\/ min-2, determined by measuring the turbidity\nincrease at 650 nm due to insulin reduction.The activity is expressed as the ratio of the slope of a \nlinear part of the turbidity curve to the lag time.\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:\u0026gt;95% as determined by SDS-PAGE and HPLC.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:Less than 1.0 EU\/µg as determined by LAL method.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eForm\u003c\/b\u003e:Lyophilized powder\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBuffer\u003c\/b\u003e:Lyophilized from a  0.2 µm filtered  PBS, pH 7.0\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg\/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?\/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eNotes\u003c\/b\u003e:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAlternative Name\/ Alias\u003c\/b\u003e:PDI, Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase subunit beta, p55,\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRelevance\u003c\/b\u003e:This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form\/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. {ECO:0000269|PubMed:10636893, ECO:0000269|PubMed:12485997}.\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePubMed ID\u003c\/b\u003e:3034602; 3611107; 2846539; 14702039; 15489334; 1597478; 9150948; 12665801; 2079031; 1286669; 9399589; 3342239; 1699755; 10636893; 11181151; 12485997; 12095988; 12218051; 12218052; 12643545; 15158710; 14592831; 15644496; 17081065; 21269460; 24275569; 25683117; 8580850; 8672469; 10383197;\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFunction\u003c\/b\u003e:This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form\/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration \u003c\/p\u003e\u003cp\u003e\u003cb\u003eInvolvement in disease\u003c\/b\u003e:Cole-Carpenter syndrome 1 (CLCRP1) \u003c\/p\u003e\u003cp\u003e\u003cb\u003eSubcellular Location\u003c\/b\u003e:Endoplasmic reticulum, Endoplasmic reticulum lumen, Melanosome, Cell membrane, Peripheral membrane protein\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProtein Families\u003c\/b\u003e:Protein disulfide isomerase family\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTissue Specificity\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePaythway\u003c\/b\u003e:Proteinprocessinginendoplasmicreticulum\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHGNC Database Link\u003c\/b\u003e:https:\/\/www.genenames.org\/cgi-bin\/gene_symbol_report?hgnc_id=HGNC:8548\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniGene Database Link\u003c\/b\u003e:https:\/\/www.ncbi.nlm.nih.gov\/UniGene\/clust.cgi?ORG=Hs\u0026amp;CID=464336\u003c\/p\u003e\u003cp\u003e\u003cb\u003eKEGG Database Link\u003c\/b\u003e:https:\/\/www.genome.jp\/dbget-bin\/www_bget?hsa:5034\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSTRING Database Link\u003c\/b\u003e:https:\/\/string-db.org\/network\/9606.ENSP00000327801\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOMIM Database Link\u003c\/b\u003e:https:\/\/www.omim.org\/entry\/112240112240112240\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":40529901289572,"sku":"CSB-AP000091HU","price":673.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/no_image_default_image-jpeg_fa6c2e14-fc22-4acb-888b-4977385aab71.jpg?v=1661105883","url":"https:\/\/www.genebiosystems.com\/en-us\/products\/recombinant-human-protein-disulfide-isomerase-proteinp4hb-active","provider":"GeneBio ","version":"1.0","type":"link"}