{"product_id":"recombinant-human-cdk2-protein-e-coli-his-tag","title":"Recombinant Human CDK2 Protein (E.coli, His Tag)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:100μg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShipping\u003c\/b\u003e:This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExp date\u003c\/b\u003e:12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_II\u003c\/b\u003e:Recombinant Proteins\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_III\u003c\/b\u003e:Others\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e:CDK2\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Synonym\u003c\/b\u003e:CDK2;CDKN2;Cell Division Protein Kinase 2;Cyclin-Dependent Kinase 2;p33 Protein Kinase\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e:Signal Transduction;Cell biology;Neuroscience;Cancer;epigenetics and nuclear signal;metabolism;\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e:Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExpression Host\u003c\/b\u003e:E.coli\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFusion tag\u003c\/b\u003e:N-His\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUNIProt ID\u003c\/b\u003e:P24941\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e:P24941\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e:Cyclin-dependent kinase 2 (CDK2) belongs to the cyclin-dependent kinase of Ser\/Thr protein kinase. CDK2 acts as a catalytic subunit of the cyclin dependent kinase complex, whose activity is restricted to the G1-S phage of the cell cycle, it is essential for the G1\/S transition. The kinase activity of CDK2 can be regulated by the association with a cyclin subunit, its phosphorylation state and CDK inhibitors. The activation of the CDK2\/cyclin complex requires the phosphorylation of Thr160 and the dephosphorylation of Try14 and Tyr15. The inhibition of CDK2-cyclin complex can also be attributed to association with p27Kip1 and p21Waf1\/Cip1. The activation of CDK2 has been shown to be necessary for apoptosis of quiescent cells, such as neurons, thymocytes and endothelial cells.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eActivity\u003c\/b\u003e:Not validated for activity\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e:Met 1-Leu298\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e: \u0026gt; 90% as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e:Lyophilized from sterile PBS, pH 7.4., 5% trehalose, 5% mannitol, 0.01% Tween 80.\u003cbr\u003eNormally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.\u003cbr\u003ePlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg\/mL. Concentration is measured by UV-Vis.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:\u0026lt; 10 EU\/mg of the protein as determined by the LAL method.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated MW\u003c\/b\u003e:36.1 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObservedMW\u003c\/b\u003e:34 kDa\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48644099407972,"sku":"PDEH100671","price":1017.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_01027e61-b8d5-438b-adad-e948bf60df23.jpg?v=1782612018","url":"https:\/\/www.genebiosystems.com\/en-us\/products\/recombinant-human-cdk2-protein-e-coli-his-tag","provider":"GeneBio ","version":"1.0","type":"link"}