{"product_id":"recombinant-human-ak4-ak3l1-protein-his-gst-tag","title":"Recombinant Human AK4\/AK3L1 Protein (His \u0026 GST Tag)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 50μg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShipping\u003c\/b\u003e: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExp date\u003c\/b\u003e: 12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_II\u003c\/b\u003e: Recombinant Proteins\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_III\u003c\/b\u003e: Others\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Synonym\u003c\/b\u003e: AK3;AK3L1;AK3L2;AK4\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e: Signal Transduction,Metabolism\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e: Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExpression Host\u003c\/b\u003e: Baculovirus-Insect Cells\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eFusion tag\u003c\/b\u003e: N-His-GST\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUNIProt ID\u003c\/b\u003e: P27144\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e: P27144-1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: Adenylate kinase isoenzyme 4; mitochondrial; also known as  ATP-AMP transphosphorylase; Adenylate kinase 3-like; AK4 and AK3L1; is a member theadenylate kinase  family. AK4 \/ AK3L1 is localized to the mitochondrial matrix. Adenylate kinases  regulate the adenine and guanine nucleotide compositions within a cell by  catalyzing the reversible transfer of phosphate group among these nucleotides.  Five isozymes of adenylate kinase have been identified in vertebrates. Expression of  these isozymes is tissue-specific and  developmentally regulated. AK4 \/ AK3L1 catalyzes the reversible transfer of the  terminal phosphate group between ATP and AMP. It may also be active with GTP. Adenylate kinase 4  ( AK4 \/ AK3L1 ) is a unique member with no enzymatic activity in the adenylate kinase  (AK) family although it shares high sequence homology with other AKs. It  remains unclear what physiological function AK4 might play or why it is enzymatically inactive. AK4 \/ AK3L1 retains the  capability of binding nucleotides. It has a glutamine residue instead of a key arginine residue in the active site well  conserved in other AKs. The enzymatically inactive AK4 is a stress responsive  protein critical to cell survival and proliferation. AK4 \/ AK3L1 is likely that  the interaction with the mitochondrial inner membrane protein ANT is important  for AK4 to exert the protective benefits to cells under stress. AK4 \/ AK3L1  also acts on the specific mechanism of energy metabolism rather than control of  the homeostasis of the ADP pool ubiquitously.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eActivity\u003c\/b\u003e: Not validated for activity\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e: Ala 2-Tyr 223\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:  \u0026gt; 94 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 8.0\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e: Not Applicable\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:  \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated MW\u003c\/b\u003e: 53.0 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObservedMW\u003c\/b\u003e: 46 kDa\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":47972817698916,"sku":"PKSH030336","price":879.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_bd35207e-ed50-44fc-956a-9f589a9a54ce.jpg?v=1772197764","url":"https:\/\/www.genebiosystems.com\/en-us\/products\/recombinant-human-ak4-ak3l1-protein-his-gst-tag","provider":"GeneBio ","version":"1.0","type":"link"}