{"product_id":"rat-peroxisome-proliferator-activated-receptor-beta-ppar-b-ppard-elisa-kit","title":"Rat Peroxisome proliferator-activated receptor beta (PPAR-B\/PPARD) ELISA Kit","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 96T. Other sizes are also available. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecies Reactivity\u003c\/b\u003e: Rat (Rattus norvegicus)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniProt\u003c\/b\u003e: N\/A\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e: PPAR-B\/PPARD\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAlternative Names\u003c\/b\u003e: FAAR; MGC3931; NR1C2; NUC1; NUCI; NUCII; PPAR-beta; PPARB; OTTHUMP00000016256|OTTHUMP00000016257|nuclear hormone receptor 1|peroxisome proliferative activated receptor; delta\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: ELISA\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRange\u003c\/b\u003e: Request Information\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSensitivity\u003c\/b\u003e: Request Information\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTest principle\u003c\/b\u003e: This assay employs a two-site sandwich ELISA to quantitate PPAR-B\/PPARD in samples. An antibody specific for PPAR-B\/PPARD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPAR-B\/PPARD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPAR-B\/PPARD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPAR-B\/PPARD bound in the initial step. The color development is stopped and the intensity of the color is measured.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Overview\u003c\/b\u003e: Peroxisome proliferator-activated receptor deltais a member of the peroxisome proliferator-activated receptor (PPAR) family. It was first identified in Xenopus in 1993.PPAR-δ\/β is the main target of a chemical substance named GW 501516. Using this ligand as a research ligand, It has been shown that agonism of PPAR-δ changes the body's fuel preference from glucose to lipids. PPAR-δ\/β is highly expressed in colon, small intestine, liver and keratinocytes. In contrast, heart, spleen, skeletal muscle, lung, brain and thymus show low expression.Knockout mice lacking the ligand binding domain of PPAR-δ\/β are viable. However these mice are smaller than the wild type both neo and postnatally. In addition, fat stores in the gonads of the mutants are smaller.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStability\u003c\/b\u003e: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. \nThe loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":40500710342756,"sku":"AE62806RA","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/no_image_default_image-jpeg_50215a96-5e1d-4506-8a2f-e55d1b618129.jpg?v=1660529577","url":"https:\/\/www.genebiosystems.com\/en-us\/products\/rat-peroxisome-proliferator-activated-receptor-beta-ppar-b-ppard-elisa-kit","provider":"GeneBio ","version":"1.0","type":"link"}