{"product_id":"pvdf-membrane-0-2-mm","title":"PVDF Membrane (0.2 μm)","description":"\u003ch2\u003e\u003cstrong\u003e\u003cspan\u003eProduct Introduction\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis product PVDF membrane is polyvinylidene fluoride membrane, which has high mechanical strength and is an ideal solid support for western blotting. PVDF membrane is hydrophobic, and the pore size of the membrane is large or small. The smaller the pore size of the membrane, the stronger the binding of proteins with low molecular weight. According to the pore size of the membrane, there are generally different specifications such as 0.1 μm, 0.22 μm, 0.45 μm and 1.0 μm. The 0.22 μm PVDF membrane is mainly used for low molecular weight Western blotting, amino acid analysis and N-terminal protein sequencing.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003ePackaging\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis product has a 0.22 μm aperture, a cut size of 7×9 cm, and a pack of 10 sheets. Each membrane can be used for transfer of commonly used gel blocks.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003e\u003cspan\u003eStorage and Transportation\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eTransport at room temperature, keep dry and sealed to prevent moisture. long term effective.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003e\u003cspan\u003eInstructions for Use\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e1. A membrane with a pore size of 0.45 μm is recommended for proteins with a molecular weight greater than 20 kDa, and a 0.22 μm membrane for proteins with a molecular weight of less than 20 kDa.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. The PVDF membrane needs to be pretreated with methanol before use to activate the positively charged groups on the membrane, making it easier to bind to negatively charged proteins.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003e\u003cspan\u003eNotice\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e1. \u003c\/span\u003e\u003cspan\u003eThe PVDF membrane is easy to dry and should be placed in TBST as soon as possible after transfer. If the membrane is too dry, it is recommended to reactivate it with methanol.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. \u003c\/span\u003e\u003cspan\u003ePlease wear a lab coat and disposable gloves during operation.\u003c\/span\u003e\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":45584519594084,"sku":"G6045-0.22","price":139.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_32b0f019-52e3-4f21-a000-5243c32306a5.jpg?v=1743090710","url":"https:\/\/www.genebiosystems.com\/en-us\/products\/pvdf-membrane-0-2-mm","provider":"GeneBio ","version":"1.0","type":"link"}