{"product_id":"purified-rabbit-anti-crk-py221-antibody","title":"Purified Rabbit anti-CRK (pY221) Antibody","description":"\u003cp\u003e\u003cb\u003eSize 1\u003c\/b\u003e: 100 ul\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSynonyms \u003c\/b\u003e: Adapter molecule crk; Proto-oncogene c-Crk; p38\u003c\/p\u003e\u003cp\u003e\u003cb\u003ehost\u003c\/b\u003e: Rabbit\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）\u003c\/b\u003e: H, M, R\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplications\u003c\/b\u003e: WB, IF\/IC\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: Rabbit polyclonal antibody to CRK (pY221)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: 1mg\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eImmunogen\u003c\/b\u003e: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CRK. The exact sequence is proprietary.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWB description\u003c\/b\u003e: Western blot analysis of CRK (pY221) expression in Hela (A), THP1 (B), mouse liver (C), rat liver (D) whole cell lysates.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIHC description\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eIF\/ICC description\u003c\/b\u003e: Immunofluorescent analysis of CRK (pY221) staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIP description\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eFC description\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eChIP description\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurification_Method\u003c\/b\u003e: The antibody was purified by immunogen affinity chromatography.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality\u003c\/b\u003e: Polyclonal\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eBuffer\u003c\/b\u003e: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDilution\u003c\/b\u003e: WB (1\/500 - 1\/1000), IF\/IC (1\/100 - 1\/500)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene ID(Human)\u003c\/b\u003e: 1398\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene ID (Mouse)\u003c\/b\u003e: 12928\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene ID(Rat)\u003c\/b\u003e: 54245\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSwissProt (Human)\u003c\/b\u003e: P46108\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSwissProt (Mouse)\u003c\/b\u003e: Q64010\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSwissProt (Rat)\u003c\/b\u003e: Q63768\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Store at -20℃. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48738509193316,"sku":"DL94738A","price":754.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_b48a64f2-c046-4d31-8cf3-89b29158bd28.jpg?v=1783811122","url":"https:\/\/www.genebiosystems.com\/en-us\/products\/purified-rabbit-anti-crk-py221-antibody","provider":"GeneBio ","version":"1.0","type":"link"}