{"product_id":"phospho-smad3-thr179-antibody","title":"Phospho-Smad3 (Thr179) Antibody , Cat. #  AF3363","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:100ul\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHost\u003c\/b\u003e:Rabbit\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality\u003c\/b\u003e:Polyclonal\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e:IgG\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGeneName\u003c\/b\u003e:SMAD3\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGeneID\u003c\/b\u003e:4088\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniprot\u003c\/b\u003e:P84022\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e:Phospho-Smad3 (Thr179) Antibody detects endogenous levels of Smad3 only when phosphorylated at Threonine 179. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eImmunogen\u003c\/b\u003e:A synthesized peptide derived from human Smad3 around the phosphorylation site of Thr179.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e:Human,Mouse,Rat\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePrediction\u003c\/b\u003e:Pig,Bovine,Horse,Sheep,Dog,Chicken,Xenopus\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e:WB,IHC,IF\/ICC,ELISA(peptide)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDilution\u003c\/b\u003e:WB 1:500-1:2000, IHC 1:50-1:200, IF\/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugate\u003c\/b\u003e:Unconjugated\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRRID\u003c\/b\u003e:AB_2834778\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAn Introduction to Blocking Peptides\u003c\/b\u003e:A superior strategy for validation of antibody binding is the use of blocking peptides.  In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCat# Blocking Peptide\u003c\/b\u003e:AF3363-BP\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDes# Blocking Peptide\u003c\/b\u003e:Phospho-Smad3 (Thr179) Blocking peptide\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBlocking Peptide Price\u003c\/b\u003e:$549 CAD\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":40527449981028,"sku":"AF3363","price":489.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/no_image_default_image-jpeg_a560bc75-eeae-433d-bf3d-67c9c9999727.jpg?v=1661028910","url":"https:\/\/www.genebiosystems.com\/en-us\/products\/phospho-smad3-thr179-antibody","provider":"GeneBio ","version":"1.0","type":"link"}