{"product_id":"phospho-nephrin-tyr1176-antibody","title":"Phospho-Nephrin (Tyr1176) Antibody  , Cat. #  AF7451","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:100ul\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHost\u003c\/b\u003e:Rabbit\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality\u003c\/b\u003e:Polyclonal\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e:IgG\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGeneName\u003c\/b\u003e:NPHS1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGeneID\u003c\/b\u003e:4868\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniprot\u003c\/b\u003e:O60500\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e:Phospho-Nephrin (Tyr1176) Antibody  detects endogenous levels of Nephrin only when phosphorylated at Tyr1176. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eImmunogen\u003c\/b\u003e:A synthesized peptide derived from human Nephrin around the phosphorylation site of Tyr1176.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e:Human,Mouse\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePrediction\u003c\/b\u003e:Pig,Bovine,Horse,Sheep,Rabbit,Dog\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e:WB,ELISA(peptide)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDilution\u003c\/b\u003e:WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugate\u003c\/b\u003e:Unconjugated\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRRID\u003c\/b\u003e:AB_2843891\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAn Introduction to Blocking Peptides\u003c\/b\u003e:A superior strategy for validation of antibody binding is the use of blocking peptides.  In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCat# Blocking Peptide\u003c\/b\u003e:AF7451-BP\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDes# Blocking Peptide\u003c\/b\u003e:Phospho-Nephrin (Tyr1176) Blocking peptide \u003c\/p\u003e\u003cp\u003e\u003cb\u003eBlocking Peptide Price\u003c\/b\u003e:$549 CAD\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":40527546548324,"sku":"AF7451","price":592.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/no_image_default_image-jpeg_257ddae4-0068-49d5-ae8b-6215a5c803e1.jpg?v=1661031729","url":"https:\/\/www.genebiosystems.com\/en-us\/products\/phospho-nephrin-tyr1176-antibody","provider":"GeneBio ","version":"1.0","type":"link"}