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Features: • 5 minutes fast ligation of Pfu-amplified PCR products. • Ampicillin resistance. • T7 promoter primer and T7 terminator primer for sequencing. • Bacteriophage T7lac promoter for high level expression. • N-terminal 6×His tag for easy purification. • Trans1-T1 phage resistant chemically competent cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing. • E1 Expression Plasmid included as negative control. |
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Storage |
Competent Cell at -70°C for six months; others at -20°C for nine months |
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Kit Content |
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Component |
CE111-01 (10 rxns) |
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pEASY®-Blunt E1 Expression Vector (15 ng/μl) |
10 µl |
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E1 Expression Plasmid (Negative Control) (15 ng/μl) |
10 µl |
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EControl Template (5 ng/μl) |
10 µl |
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EControl Forward Primer (10 μM) |
10 µl |
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EControl Reverse Primer (10 μM) |
10 µl |
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T7 Promoter Primer (10 μM) |
50 µl |
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T7 Terminator Primer (10 μM) |
50 µl |
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Trans1-T1 Phage Resistant Chemically Competent Cell |
5×100 μl |
Papers citing this product:
| Literature | Journal | IF | Author | Date | Link |
|---|---|---|---|---|---|
| A Viral Satellite DNA Vector (TYLCCNV) for Functional Analysis of miRNAs and siRNAs in Plants | Plant Physiology | 7.367 | Dongyan Cao, et al. College of Food Science and Nu | 2017 Apr | The original link |
| Identification of a novel class B scavenger receptor homologue in Portunus trituberculatus: Molecular cloning and microbial ligand binding | fish & shellfish immunology | 3.025 | Yang N, et al. Key Laboratory of the Ministry of E | 2016 Nov | The original link |
| Genome-wide identification and functional analysis of S-RNase involved in the self-incompatibility of citrus | molecular genetics and genomics | 2.622 | Liang M, et al. Key Laboratory of Horticultural Pl | 2016 Dec | The original link |
| Functional characterization of aphid OR | insect science | 2.026 | Rui-Bin Zhang,et al. | 2017Jul | The original link |
| Characterization of a neutral recombinant xylanase from Thermoactinospora rubra YIM 77501T | antonie van leeuwenhoek international journal of g | 1.944 | Yin YR, et al. Yunnan Institute of Microbiology, Y | 2017 Mar | The original link |