{"product_id":"mouse-biotin-elisa-kit","title":"Mouse Biotin ELISA Kit","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:96T\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e:Mouse\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAlternative Names\u003c\/b\u003e:Biotin\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay Type\u003c\/b\u003e:Competitive Inhibition\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSensitivity\u003c\/b\u003e:4.7 pg\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStandard\u003c\/b\u003e:1000 pg\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDetection range\u003c\/b\u003e:15.63-1000 pg\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSample type\u003c\/b\u003e:Serum, plasma, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay length\u003c\/b\u003e:2h\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Area\u003c\/b\u003e:Enzyme \u0026amp; Kinase;\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTest principle\u003c\/b\u003e:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse Biotin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Biotin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Biotin in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniProt ID\u003c\/b\u003e:\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48644957044836,"sku":"ELK10917","price":1021.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_383eb065-cbea-4be3-96ad-c0ec0701beae.jpg?v=1782662073","url":"https:\/\/www.genebiosystems.com\/en-us\/products\/mouse-biotin-elisa-kit","provider":"GeneBio ","version":"1.0","type":"link"}