{"product_id":"human-e3-ubiquitin-protein-ligase-amfr-amfr-elisa-kit","title":"Human E3 Ubiquitin-protein Ligase Amfr, AMFR ELISA Kit","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:96 Tests-1 Kit\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e:E3 ubiquitin-protein ligase that mediates the polyubiquitination of lysine and cysteine residues on target proteins, such as CD3D, CYP3A4, CFTR, INSIG1, SOAT2\/ACAT2 and APOB for proteasomal degradation (PubMed: 10456327, PubMed: 11724934, PubMed: 12670940, PubMed: 19103148, PubMed: 24424410, PubMed: 28604676). Component of a VCP\/p97-AMFR\/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD) (PubMed: 10456327, PubMed: 11724934, PubMed: 19103148, PubMed: 24424410). The VCP\/p97-AMFR\/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG1 complex at the ER membrane (PubMed: 16168377, PubMed: 22143767). In addition, interaction of AMFR with AUP1 facilitates interaction of AMFR with ubiquitin-conjugating enzyme UBE2G2 and ubiquitin ligase RNF139, leading to sterol-induced HMGCR ubiquitination (PubMed: 23223569). The ubiquitinated HMGCR is then released from the ER into the cytosol for subsequent destruction (PubMed: 16168377, PubMed: 22143767, PubMed: 23223569). In addition to ubiquitination on lysine residues, catalyzes ubiquitination on cysteine residues: together with INSIG1, mediates polyubiquitination of SOAT2\/ACAT2 at 'Cys-277', leading to its degradation when the lipid levels are low (PubMed: 28604676). Catalyzes ubiquitination and subsequent degradation of INSIG1 when cells are depleted of sterols (PubMed: 17043353). Mediates polyubiquitination of INSIG2 at 'Cys-215' in some tissues, leading to its degradation (PubMed: 31953408). Also regulates ERAD through the ubiquitination of UBL4A a component of the BAG6\/BAT3 complex (PubMed: 21636303). Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation (PubMed: 21636303). Mediates tumor invasion and metastasis as a receptor for the GPI\/autocrine motility factor (PubMed: 10456327). In association with LMBR1L and UBAC2, negatively regulates the canonical Wnt signaling pathway in the lymphocytes by promoting the ubiquitin-mediated degradation of CTNNB1 and Wnt receptors FZD6 and LRP6 (PubMed: 31073040).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch_area\u003c\/b\u003e:Cell Biology\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplications\u003c\/b\u003e:ELISA\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget_protein\u003c\/b\u003e:AMFR\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e:Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay type\u003c\/b\u003e:Sandwich\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSensitivity\u003c\/b\u003e:7.15ng\/L\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDetection range\u003c\/b\u003e:15-3000ng\/L\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSample type\u003c\/b\u003e:Serum, plasma or other biological fluids\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFull product name\u003c\/b\u003e:E3 ubiquitin-protein ligase AMFR\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecies\u003c\/b\u003e:Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e:2-8ºC\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay time\u003c\/b\u003e:1h 30m\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniProt accession\u003c\/b\u003e:Q9UKV5\u003c\/p\u003e\u003cp\u003e\u003cb\u003eMASS(Da)\u003c\/b\u003e:72,996\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGeneID\u003c\/b\u003e:267\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSynonyms\u003c\/b\u003e:AMF receptor;AMFR;Autocrine motility factor receptor;E3 ubiquitin-protein ligase AMFR;gp78;RING finger protein 45\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene names\u003c\/b\u003e:AMFR\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStandard solution\u003c\/b\u003e:3200ng\/L\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48648118173796,"sku":"E5210Hu","price":848.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_b2b13e07-298a-445b-a361-3520b47bb8ce.jpg?v=1782709413","url":"https:\/\/www.genebiosystems.com\/en-us\/products\/human-e3-ubiquitin-protein-ligase-amfr-amfr-elisa-kit","provider":"GeneBio ","version":"1.0","type":"link"}