{"product_id":"human-1-25-hydroxyvitamin-d-1-25-oh-d-cyp24a1-elisa-kit","title":"Human 1, 25-Hydroxyvitamin D,1, 25-(OH)D, CYP24A1 ELISA Kit","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:96 Tests-1 Kit\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e:A cytochrome P450 monooxygenase with a key role in vitamin D catabolism and calcium homeostasis. Via C24- and C23-oxidation pathways, catalyzes the inactivation of both the vitamin D precursor calcidiol (25-hydroxyvitamin D(3)) and the active hormone calcitriol (1-alpha,25-dihydroxyvitamin D(3)) (PubMed: 24893882, PubMed: 15574355, PubMed: 8679605, PubMed: 11012668, PubMed: 16617161, PubMed: 29461981). With initial hydroxylation at C-24 (via C24-oxidation pathway), performs a sequential 6-step oxidation of calcitriol leading to the formation of the biliary metabolite calcitroic acid (PubMed: 24893882, PubMed: 15574355). With initial hydroxylation at C-23 (via C23-oxidation pathway), catalyzes sequential oxidation of calcidiol leading to the formation of 25(OH)D3-26,23-lactone as end product (PubMed: 11012668, PubMed: 8679605). Preferentially hydroxylates at C-25 other vitamin D active metabolites, such as CYP11A1-derived secosteroids 20S-hydroxycholecalciferol and 20S,23-dihydroxycholecalciferol (PubMed: 25727742). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR\/adrenodoxin reductase and FDX1\/adrenodoxin (PubMed: 8679605).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch_area\u003c\/b\u003e:Cancer\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplications\u003c\/b\u003e:ELISA\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget_protein\u003c\/b\u003e:CYP24A1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e:Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay type\u003c\/b\u003e:Sandwich\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSensitivity\u003c\/b\u003e:3.14pmol\/L\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDetection range\u003c\/b\u003e:7-1500pmol\/L\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSample type\u003c\/b\u003e:Serum, plasma or other biological fluids\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFull product name\u003c\/b\u003e:1,25-Hydroxyvitamin D\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecies\u003c\/b\u003e:Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e:2-8ºC\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay time\u003c\/b\u003e:1h 30m\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniProt accession\u003c\/b\u003e:Q07973\u003c\/p\u003e\u003cp\u003e\u003cb\u003eMASS(Da)\u003c\/b\u003e:58,875\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGeneID\u003c\/b\u003e:1591\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSynonyms\u003c\/b\u003e:1,25-dihydroxyvitamin D,3 24-hydroxylase, mitochondrial;24-OHase;CYP 24A1;CYP24A1;Cytochrome P450 24A1;Cytochrome P450-CC24;Vitamin D, 3 24-hydroxylase\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene names\u003c\/b\u003e:CYP24A1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStandard solution\u003c\/b\u003e:1600pmol\/L\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48648044413028,"sku":"E1919Hu","price":848.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_088381b7-8c64-4e83-8865-bc1ed850f861.jpg?v=1782706680","url":"https:\/\/www.genebiosystems.com\/en-us\/products\/human-1-25-hydroxyvitamin-d-1-25-oh-d-cyp24a1-elisa-kit","provider":"GeneBio ","version":"1.0","type":"link"}