Streamlined isolation / extraction of total RNA from a large number of mammlian tissues and cells and yeast.
- Provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100ug RNA.
- No need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl are eliminated
- Applications: RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation
Storage/Stability: store kit dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
Protocol can be found: here: https://cdn.shopify.com/s/files/1/0558/8588/9636/files/R4011_HiPure_Total_RNA_Kit-GBS.pdf?v=1670444038
HiPure RNA Mini Columns
|2 mL Collection Tubes||50||250|
|RTL Lysis Buffer||50 mL||200 mL|
|RNA Binding Buffer||15 mL||75 mL|
|Buffer RW1||50 mL||200 mL|
|12 mL||2 x 50 mL|
|RNase Free Water||10 mL||30 mL|