This product is a 2X Fast DNA reagent Mix, plus a separately supplied Fast DNA Polymerase. The mix contains dNTPs, Mg2+ and Buffer at optimal concentrations for efficient PCR amplification. This Optimzer format provides flexibility of optimizing Fast DNA polymerase activity for each unique reaction and still the convenience that comes with master mixes. Fast DNA polymerase is >2x faster than Taq
Fast Taq DNA polymerase is the latest generation Taq-based DNA polymerase. It possesses high amplification efficiency as does Taq polymerase, and fast elongation ability as does KOD polymerase, can be used in a variety of PCR. The FSTM PCR Buffer, designed for FSTM Taq DNA polymerase, can be used in fast amplification reaction. The elongation rate of FSTM Taq DNA polymerase is 2 more than twice the rate by regular Taq DNA polymerase, which shortens the amplification time at least by half .
Fast rate of elongation: elongation rate can reach to 3 kb/min, more than twice the rate by regular Taq DNA polymerase;
Highly thermostable : have a half-life of over 40 min at 95°C incubation Generates 3'-dA overhangs PCR products.
Generate PCR product for TA cloning
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.
The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases is confirmed by appropriate quality tests
Functionally tested in amplification of a single-copy gene from human genomic DNA
Store all components at –20°C
Protocol for PCR
All solutions should be thawed on ice, gently vortexed and briefly centrifuged.
Add in a thin walled PCR tube on ice: