EasyPure® Bacteria Genomic DNA Kit

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EasyPure® Bacteria Genomic DNA Kit

EE161-01
Regular price
$123.00 USD
Sale price
$123.00 USD
Regular price
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Product Details

EasyPure® Bacteria Genomic DNA Kit uses lysozyme and moderate lysis buffer to lyse cells. Proteinase K is used for protein digestion and RNase A used for RNA digestion. DNA is specifically bound to silica-based column in hypersaline condition, and DNA is eluted by low salt and high pH solution. This kit is suitable for isolating high quality genomic DNA from Gram-positive and Gram-negative bacteria. The isolated DNA is suitable for PCR, restriction enzyme digestion, and Southern blot.

  • Fast: the whole process can be completed in 50 minutes.
  • High yield: DNA yield up to 20 μg.
  • Includes RNAase A and Proteinase K.
    • Lysozyme not included
  • Sample size required: ≤1x10^9 Gram-positive or Gram-negative cells

Applications

PCR, restriction enzyme digestion and Southern blot

Storage

at room temperature (15-25°C) in a dry place for one year.


Shipping

at room temperature.



Product Contents


Component

EE161-01 

(50 rxns)

Resuspension Buffer11 (RB11) 12 ml
Lysis Buffer11 (LB11) 6 ml
Binding Buffer11 (BB11) 10 ml
Clean Buffer 11 (CB11) 55 ml
Wash Buffer 11 (WB11) 12 ml
Elution Buffer (EB) 25 ml
RNase A (10 mg/ml) 1 ml
Proteinase K (20 mg/ml) 1 ml
Genomic Spin Columns with Collection Tubes 50 each



Citations

Liu Y, Chen Z, Jiang Z, et al. Biochemical characterization of a novel L-Asparaginase from Paenibacillus barengoltzii being suitable for acrylamide reduction in potato chips and mooncakes[J]. International Journal of Biological Macromolecules,2017, 104(Pt A):1055-1063. 

Shi R , Liu Y , Mu Q , et al. Biochemical characterization of a novel L-asparaginase from Paenibacillus barengoltzii being suitable for acrylamide reduction in potato chips and mooncakes[J]. International Journal of Biological Macromolecules, 2017, 96:93-99.

Zheng X , Liu H , Song L , et al. Phaeocystidibacter marisrubri sp. nov. a member of the family Cryomorphaceae isolated from Red Sea sediment[J]. International Journal of Systematic and Evolutionary Microbiology, 2015, 65(7):2199.

Duan D , Cheng T . Determination of the microbial community features of Haemaphysalis flava in different developmental stages by high‐throughput sequencing[J]. Journal of Basic Microbiology, 2016, 57(4):302.

Cheng T Y , Liu G H . PCR denaturing gradient gel electrophoresis as a useful method to identify of intestinal bacteria flora in Haemaphysalis flava ticks[J]. Acta Parasitologica, 2017, 62(2):269-272.

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