{"product_id":"anti-c-kit-antibody-picoband-r","title":"Anti-c-Kit Antibody Picoband®","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 100 μg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eForm\u003c\/b\u003e: Lyophilized\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e: Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplications\u003c\/b\u003e: Flow Cytometry,IHC,ICC,WB\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication Details\u003c\/b\u003e: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg\/ml\u003cbr\u003eImmunohistochemistry (Frozen Section), 0.5-1μg\/ml\u003cbr\u003e\nImmunocytochemistry, 0.5-1μg\/ml\u003cbr\u003eWestern blot, 0.1-0.5μg\/ml\u003cbr\u003eFlow Cytometry (Fixed), 1-3μg\/1x10\u003csup\u003e6\u003c\/sup\u003ecells\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene Name\u003c\/b\u003e: KIT\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: No cross reactivity with other proteins.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: SCFR (Mast\/stem cell growth factor receptor), also known as KIT ONCOGENE or CD117, is a protein that in humans is encoded by the KIT gene. KIT was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. The KIT gene is mapped on 4q12. Kit was expressed on the surface of germ cells up to the pachytene stage. Signaling from the KIT receptor tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the KIT effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eImmunogen\u003c\/b\u003e: E.coli-derived human c-Kit recombinant protein (Position: Q26-S285). Human c-Kit shares 66% amino acid (aa) sequence identity with mouse c-Kit.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality\u003c\/b\u003e: Polyclonal\u003c\/p\u003e\u003cp\u003e\u003cb\u003eContents\u003c\/b\u003e: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.\u003cbr\u003e\n*This antibody is supplied in a stabilized formulation. \u003cbr\u003e\nCompatibility with conjugation reactions depends on the chemistry of the conjugation method used. \u003cbr\u003e\nFor conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurification\u003c\/b\u003e: Immunogen affinity purified.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e: Add 0.2ml of distilled water will yield a concentration of 500ug\/ml.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReference\u003c\/b\u003e: 1. Barker, P. E., Besmer, P., Ruddle, F. H. Human c-kit oncogene on human chromosome 4. (Abstract) Am. J. Hum. Genet. 37: A143, 1985.\n2. Chen, L. L., Sabripour, M., Wu, E. F., Prieto, V. G., Fuller, G. N., Frazier, M. L. A mutation-created novel intra-exonic pre-mRNA splice site causes constitutive activation of KIT in human gastrointestinal stromal tumors. Oncogene 24: 4271-4280, 2005.\n3. Andre C, Hampe A, Lachaume P, Martin E, Wang XP, Manus V, Hu WX, Galibert F (January 1997). Sequence analysis of two genomic regions containing the KIT and the FMS receptor tyrosine kinase genes. Genomics 39(2): 216–26.\n4. Yarden Y, Kuang WJ, Yang-Feng T, Coussens L, Munemitsu S, Dull TJ, Chen E, Schlessinger J, Francke U, Ullrich A (November 1987). Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand. EMBO J. 6 (11): 3341–51.\n\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniprot ID\u003c\/b\u003e: P10721\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHost\u003c\/b\u003e: Rabbit\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: Adding 0.2 ml of distilled water will yield a concentration of 500 μg\/ml.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugate\u003c\/b\u003e: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCross Reactivity\u003c\/b\u003e: No cross-reactivity with other proteins\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e: Rabbit IgG\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePhospho_site\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone Number\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eObserved Molecular Weight\u003c\/b\u003e: \n109 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated Molecular Weight\u003c\/b\u003e: 109865 MW\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene ID\u003c\/b\u003e: 3815\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProtein Name\u003c\/b\u003e: Mast\/stem cell growth factor receptor Kit\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene Full Name\u003c\/b\u003e: KIT proto-oncogene, receptor tyrosine kinase\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSynonyms\u003c\/b\u003e: C Kit; CD117; c-Kit; c-Kit\/CD117; KIT; PBT; Proto oncogene c Kit; SCFR; Tyrosine protein kinase Kit\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48787308773476,"sku":"PB9258","price":618.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_444e6767-2316-4719-904f-5b49ed8e192f.jpg?v=1784398570","url":"https:\/\/www.genebiosystems.com\/en-us\/products\/anti-c-kit-antibody-picoband-r","provider":"GeneBio ","version":"1.0","type":"link"}