2X Achiever™ Taq Master Mix is a mixture of Taq and Pfu polymerases, blends the processivity of Taq with the high fidelity of Pfu, allowing amplification of the higher fidelity and longer templates than the single-enzyme formulations. It is also a better choice for amplifying complex template, such as GC-rich and secondary structure-harboring template. And it is suitable as a direct replacement for ordinary Taq Polymerase in most applications. In addition, Using 2X Achiever™ Taq Master Mix results in 3´-dA overhangs PCR products, which can be used in TA clone.
Achiever™ Taq Master Mix is offered in two formats: with and without a blue loading dye. The inclusion of the loading dye means PCR products can be loaded directly onto an agarose gel for analysis. The dye has been validated to not interfere with PCR amplifications. This product described here contains the loading dye (pre-mixed into the 2 x master mix)
If you prefer to add your own loading dye after the completion of PCR, you can select the master mix format without loading dye pre-mixed. Achiever™ Taq Master Mix, Cat. # P211.
Higher fidelity: Its fidelity is four times higher than ordinary Taq polymerase.
High yields: Suitable for complex template which is rich in GC or repeat sequence or template containing secondary structures.
Long PCR: fragments as long as 10 kb from genomic templates and 20 kb from plasmid and phage DNA.
The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests
Functionally tested in PCR
Definition of Activity Unit
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.
Store all components at –20°C