{"title":"Viral RNA Isolation Kits","description":"\u003cp\u003e\u003cspan\u003eViral nucleic acid preparation and extraction results in high-purity DNA and RNA that can be directly used in downstream experiments ranging from reverse transcription to second-generation sequencing. The genetic material obtained from this process are critical for diagnostic testing of viral infections, genotyping studies, viral sequencing, epidemiological studies, vaccine development, antiviral drug development, viral quantification, and surveillance programs to monitor the prevalence and distribution of a specific virus.\u003c\/span\u003e\u003c\/p\u003e","products":[{"product_id":"trazol-rna-reagent","title":"TRA-zol RNA Isolation Reagent","description":"\u003cp\u003eTRA-zol Reagent is an improved version of the popular single-step total RNA isolation reagent developed by Chomczynski and Sacchi (1987). The RNA isolation (extraction) method based on this single reagent is widely used and proven for RNA applications. It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA, and proteins from samples of human, animal, plant, yeast, bacterial, and viral origin.\u003c\/p\u003e\n\u003cp\u003eTRA-zol Reagent performs well with easily scalable small or large amounts of tissue or cells, and many samples can be simultaneously extracted.\u003c\/p\u003e\n\u003cp\u003eThis product, a mixture of guanidine thiocyanate and phenol in a monophase solution, effectively dissolves DNA, RNA, and protein upon homogenization or lysis of tissue sample. After adding chloroform or 1-bromo-3-chloropropane and centrifuging, the mixture separates into 3 phases: an aqueous phase containing the RNA, the interphase containing DNA, and an organic phase containing proteins. Each component can then be isolated after separating the phases. One milliliter of TRAzol is sufficient to isolate RNA, DNA, and protein from 50-100 mg of tissue, 5-10 million cells, or 10 cm² of culture dish surface for cells grown in monolayer.\u003c\/p\u003e\n\u003cp\u003eThis is one of the most effective methods for isolating total RNA and can be completed in only 1 hour starting with fresh tissue or cells. The procedure is very effective for isolating RNA molecules of all types from 0.1-15 kb in length. The resulting RNA is intact with little or no contaminating DNA and protein.\u003c\/p\u003e\n\u003cp\u003eThe RNA extraction reagent has been widely used for isolating RNA, similar to the leading RNA Isolation Reagents. Resulting RNA can be used for Northern blots, mRNA isolation, in vitro translation, RNase protection assay, cloning, and PCR.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFeatures and Benefits:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e• Easily scalable RNA isolation • Works with many sources: human, plant, yeast, bacterial, or viral • Better yields than traditional guanidine thiocyanate\/cesium chloride methods • Cost-effective compared to other similar single-reagent RNA isolation reagents\u003c\/p\u003e\n\u003ch4\u003ePackaging\u003c\/h4\u003e\n\u003cp\u003eChoose 100, 200, 500  or 1000mL sizes. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/Trazol_Magzol_Reagent_User_Manual.pdf?v=1688853244\" title=\"Protocol for Trazol Magzol\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"221\" height=\"62\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eChomczynski, P. and Sacchi, N. Single-step method of RNA isolation by acid guanidinium thiocyanatephenol-chloroform extraction. Anal. Biochem. 162: 156-159. 1987.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Gene Bio Systems","offers":[{"title":"200mL","offer_id":39766558703716,"sku":"R1022b","price":579.4,"currency_code":"CAD","in_stock":true},{"title":"400mL","offer_id":42713521029220,"sku":"R1022c","price":966.72,"currency_code":"CAD","in_stock":true},{"title":"1.2L","offer_id":42713521061988,"sku":"R1022d","price":2611.28,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/TRAzol_20250314_105917.jpg?v=1742485128"},{"product_id":"viral-dna-rna-extraction-kit-spin-column-based","title":"Viral DNA-RNA Extraction Kit -Spin Column-based","description":"\u003cp\u003e\u003cstrong\u003e\u003cspan\u003e100\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003epreps\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe Viral DNA\/RNA Extraction Kit (Spin Column) is suitable for rapid extraction of high purity viral nucleic acid from plasma, serum, nasopharyngeal swab, sputum, bronchus\/alveolar lavage fluid, saliva, ascites, culture cell supernatant and urine. This kit is based on the silica gel column purification method. The sample is homogenized in the lysis buffer and the nucleic acid is released into the buffer. The \u003c\/span\u003e\u003cspan\u003elysis buffer\u003c\/span\u003e\u003cspan\u003e contains a high concentration of guanidine. In this condition, the membrane absorbs nucleic acid by hydrogen bond and electrostatic physical and chemical action, while proteins and other impurities are not absorbed. The lysate is transferred to the adsorption column for filtration, and the nucleic acid filter membrane is washed to remove residual proteins and other impurities, and finally eluted by the low-salt buffer solution. The obtained nucleic acids can be directly used in downstream related experiments such as reverse transcription, PCR, RT-PCR, fluorescence quantitative PCR, second-generation sequencing and Northern hybridization.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eMain components\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe kit consists of the following components:\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable cellspacing=\"0\" style=\"width: 546px;\"\u003e\n\u003ctbody\u003e\n\u003ctr class=\"firstRow\"\u003e\n\u003ctd valign=\"top\" style=\"width: 115.375px;\"\u003e\n\u003cp\u003e\u003cspan\u003eThe name of the reagent\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 113.625px;\"\u003e\n\u003cp\u003e\u003cspan\u003eAmount\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 300px;\"\u003e\n\u003cp\u003e\u003cspan\u003eC\u003c\/span\u003e\u003cspan\u003eomponent description\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" style=\"width: 115.375px;\"\u003e\n\u003cp\u003e\u003cspan\u003eLysis Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 113.625px;\"\u003e\n\u003cp\u003e\u003cspan\u003e50 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 300px;\"\u003e\n\u003cp\u003e\u003cspan\u003eProvide environment for \u003c\/span\u003e\u003cspan\u003elysing\u003c\/span\u003e\u003cspan\u003e and \u003c\/span\u003e\u003cspan\u003ebinding to the\u003c\/span\u003e\u003cspan\u003e column\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" style=\"width: 115.375px;\"\u003e\n\u003cp\u003e\u003cspan\u003eWash Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 113.625px;\"\u003e\n\u003cp\u003e\u003cspan\u003e24\u003c\/span\u003e\u003cspan\u003e ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 300px;\"\u003e\n\u003cp\u003e\u003cspan\u003eRemove residual proteins and other impurities\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" style=\"width: 115.375px;\"\u003e\n\u003cp\u003e\u003cspan\u003eElute Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 113.625px;\"\u003e\n\u003cp\u003e\u003cspan\u003e6 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 300px;\"\u003e\n\u003cp\u003e\u003cspan\u003eNuclease\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003efree solution\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" style=\"width: 115.375px;\"\u003e\n\u003cp\u003e\u003cspan\u003eSpin Column\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 113.625px;\"\u003e\n\u003cp\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003e pcs \u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003e 2\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 300px;\"\u003e\n\u003cp\u003e\u003cspan\u003eAdsorb viral nucleic acid\u003c\/span\u003e\u003cspan\u003e and collect the f\u003c\/span\u003e\u003cspan\u003eiltrate\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eStorage conditions\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStore at room temperature (15-25\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e) and transport at room temperature.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eNotes\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1. Prepare your own \u003c\/span\u003e\u003cspan\u003eRN\u003c\/span\u003e\u003cspan\u003ease-free pipette tips, 1.5 ml \u003c\/span\u003e\u003cspan\u003eRN\u003c\/span\u003e\u003cspan\u003ease-free centrifuge tube\u003c\/span\u003e\u003cspan\u003es\u003c\/span\u003e\u003cspan\u003e, centrifuge, etc.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. The virus has a strong ability to infect, \u003c\/span\u003e\u003cspan\u003ea variety of defense measures \u003c\/span\u003e\u003cspan\u003emust be done before the operation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3. Avoid repeated freezing\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003ethawing of samples, otherwise the extracted viral RNA will be degraded and the extracted amount will decrease.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4. All operating procedures, if not specified, are carried out at room temperature (15-25\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5. When using this kit, please wear lab coat, disposable latex gloves, disposable masks and use RNase-free consumables to avoid RNase pollution to the greatest extent.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e6. Please check whether there is crystal precipitation in the Lysis Buffer. If there is crystal precipitation, place it at room temperature or 37\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e until the crystal is dissolved. Mix it before use.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eBefore use:\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAdd 96 ml anhydrous ethanol to \u003c\/span\u003e\u003cspan\u003eWash Buffer\u003c\/span\u003e\u003cspan\u003e, and store at \u003c\/span\u003e\u003cspan\u003eroom temperature\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eProtocol\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1. Add 500\u003c\/span\u003e\u003cspan\u003e μl of Lysis Buffer into\u003c\/span\u003e\u003cspan\u003e a 1.5 ml RNase-free centrifuge tube (self-provided). If there are many samples, the \u003c\/span\u003e\u003cspan\u003eLysis Buffer\u003c\/span\u003e\u003cspan\u003e can be pre-packed.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. Add 200\u003c\/span\u003e\u003cspan\u003e μl\u003c\/span\u003e\u003cspan\u003e of samples, mix thoroughly by vortexing (if the sample is less than 200\u003c\/span\u003e\u003cspan\u003e μl\u003c\/span\u003e\u003cspan\u003e, fill it with normal saline to 200\u003c\/span\u003e\u003cspan\u003e μl\u003c\/span\u003e\u003cspan\u003e).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.\u003c\/span\u003e\u003cspan\u003e T\u003c\/span\u003e\u003cspan\u003eransfer the above mixture to the Spin Column \u003c\/span\u003e\u003cspan\u003e(with\u003c\/span\u003e\u003cspan\u003e Collection Tube\u003c\/span\u003e\u003cspan\u003e)\u003c\/span\u003e\u003cspan\u003e. Centrifuged at 12,000×g for 1 min.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4. Discard the filtrate and put the Spin Column back into the 2 ml Collection Tube. Add 600 \u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003el of Wash Buffer and centrifuge at 12,000×g for 30 sec, then discard the filtrate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNote: make sure the correct amount of anhydrous ethanol has been added to the Wash Buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5. Repeat step 4 once.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e6. Centrifuge for 2 min at 12,000×g to dry the column membrane. Discard the filtrate and collection tube.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e7. Transfer the Spin Column to a new 1.5 ml Collection Tube (provided by the kit), add 50 \u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003el Eluent Buffer to the center of the membrane of the Spin Column, and place it at room temperature for 1 min. Centrifuge at 12,000×g for 1 min.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e8. \u003c\/span\u003e\u003cspan\u003eDiscard the Spin Column\u003c\/span\u003e\u003cspan\u003e, the obtained DNA\/RNA can be directly used for subsequent detection, or be stored at -30 ~ -15\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e for short-term storage or at -70\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e for long-term storage.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003e\u003cbr\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39771534688356,"sku":"V4001-GB","price":214.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/v4001_image_2.jpg?v=1742214049"},{"product_id":"viral-nucleic-acid-dna-rna-miniprep-kit","title":"Viral Nucleic Acid (DNA-RNA) Miniprep Kit","description":"\u003cp\u003e\u003cspan\u003eThe kit adopts the magnetic particle purification technology based on superparamagnetism, which can minimize the risk of cross-contamination and improve the sensitivity and accuracy of detection. The operation time of the instrument is only 50 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eMain components\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe kit consists of the following components: (for a 200 prep kit)\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable cellspacing=\"0\" style=\"width: 488px;\"\u003e\n\u003ctbody\u003e\n\u003ctr class=\"firstRow\"\u003e\n\u003ctd valign=\"top\" style=\"width: 108px;\"\u003e\n\u003cp\u003e\u003cspan\u003eThe name of the reagent\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 100.927px;\"\u003e\n\u003cp\u003e\u003cspan\u003eAmount\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 262.073px;\"\u003e\n\u003cp\u003e\u003cspan\u003eC\u003c\/span\u003e\u003cspan\u003eomponent description\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" style=\"width: 108px;\"\u003e\n\u003cp\u003e\u003cspan\u003eBuffer MVL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 100.927px;\"\u003e\n\u003cp\u003e\u003cspan\u003e45\u003c\/span\u003e\u003cspan\u003e ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 262.073px;\"\u003e\n\u003cp\u003e\u003cspan\u003eProvide environment for \u003c\/span\u003e\u003cspan\u003elysing\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" style=\"width: 108px;\"\u003e\n\u003cp\u003e\u003cspan\u003eBuffer MB\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 100.927px;\"\u003e\n\u003cp\u003e\u003cspan\u003e25\u003c\/span\u003e\u003cspan\u003e ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 262.073px;\"\u003e\n\u003cp\u003e\u003cspan\u003eProvide environment for \u003c\/span\u003e\u003cspan\u003ebinding to the\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003em\u003c\/span\u003e\u003cspan\u003eagnetic \u003c\/span\u003e\u003cspan\u003eb\u003c\/span\u003e\u003cspan\u003eeads\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" style=\"width: 108px;\"\u003e\n\u003cp\u003e\u003cspan\u003eBuffer MW1\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 100.927px;\"\u003e\n\u003cp\u003e\u003cspan\u003e55\u003c\/span\u003e\u003cspan\u003e ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 262.073px;\"\u003e\n\u003cp\u003e\u003cspan\u003eRemove residual proteins and other impurities\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" style=\"width: 108px;\"\u003e\n\u003cp\u003e\u003cspan\u003eMagV Beads\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 100.927px;\"\u003e\n\u003cp\u003e\u003cspan\u003e5.2\u003c\/span\u003e\u003cspan\u003e ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 262.073px;\"\u003e\n\u003cp\u003e\u003cspan\u003eAdsorb viral nucleic acid\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" style=\"width: 108px;\"\u003e\n\u003cp\u003e\u003cspan\u003eDEPC-Water\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 100.927px;\"\u003e\n\u003cp\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003e ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 262.073px;\"\u003e\n\u003cp\u003e\u003cspan\u003eDEPC \u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003e treated\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ewater, RNase - free\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" style=\"width: 108px;\"\u003e\n\u003cp\u003e\u003cspan\u003eCarrier RNA\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 100.927px;\"\u003e\n\u003cp\u003e\u003cspan\u003e450 \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 262.073px;\"\u003e\n\u003cp\u003e\u003cspan\u003eCapture trace nucleic acid\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" style=\"width: 108px;\"\u003e\n\u003cp\u003e\u003cspan\u003eProteinase K\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 100.927px;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 ml \u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003e 2\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 262.073px;\"\u003e\n\u003cp\u003e\u003cspan\u003eLyse\u003c\/span\u003e\u003cspan\u003es\u003c\/span\u003e\u003cspan\u003e proteins\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eb\u003c\/span\u003e\u003cspan\u003eoun\u003c\/span\u003e\u003cspan\u003ed to nucleic acids\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eStorage conditions\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStore \u003c\/span\u003e\u003cspan\u003eCarrier RNA and \u003c\/span\u003e\u003cspan\u003eProteinase K \u003c\/span\u003e\u003cspan\u003eat -20\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e, \u003c\/span\u003e\u003cspan\u003eMagV Beads and DEPC-Water \u003c\/span\u003e\u003cspan\u003eat 2-8\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e, others \u003c\/span\u003e\u003cspan\u003eat room temperature (\u003c\/span\u003e\u003cspan\u003eRT, \u003c\/span\u003e\u003cspan\u003e15-25\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e)\u003c\/span\u003e\u003cspan\u003e,\u003c\/span\u003e\u003cspan\u003e and transport at \u003c\/span\u003e\u003cspan\u003eRT\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eNotes\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1. Prepare your own \u003c\/span\u003e\u003cspan\u003eRN\u003c\/span\u003e\u003cspan\u003ease-free pipette tips, 1.5 ml \u003c\/span\u003e\u003cspan\u003eRN\u003c\/span\u003e\u003cspan\u003ease-free centrifuge tube\u003c\/span\u003e\u003cspan\u003es\u003c\/span\u003e\u003cspan\u003e, centrifuge, etc.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. The virus has a strong ability to infect, \u003c\/span\u003e\u003cspan\u003ea variety of defense measures \u003c\/span\u003e\u003cspan\u003emust be done before the operation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3. Avoid repeated freezing\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003ethawing of samples, otherwise the extracted viral RNA will be degraded and the extracted amount will decrease.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4. All operating procedures, if not specified, are carried out at room temperature (15-25\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5. When using this kit, please wear lab coat, disposable latex gloves, disposable masks and use RNase-free consumables to avoid RNase pollution to the greatest extent.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e6. Please check whether there is crystal precipitation in the \u003c\/span\u003e\u003cspan\u003eBuffer MVL\u003c\/span\u003e\u003cspan\u003e. If there is crystal precipitation, place it at room temperature or 37\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e until the crystal is dissolved. Mix it before use.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eBefore use:\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAdd 55 ml anhydrous ethanol to \u003c\/span\u003e\u003cspan\u003eBuffer MW1, and store at RT\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAdd 58 ml isopropanol to \u003c\/span\u003e\u003cspan\u003eBuffer MB, and store at RT\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ePrepare a 75% ethanol solution.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eProtocol\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003e A: Manual Operation Process\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1. \u003c\/span\u003e\u003cspan\u003eViral lysis. Different lysis procedures should be adopted for different experimental materials, as follows:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eA. L\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eysis of v\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eirus in plasma, serum, cell-free body fluids, and viral stock solution\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e: prepare 10-200 \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el of plasma, serum, cell-free body fluids, or viral stock solution. If the initial amount is less than 200 \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el, use PBS solution or \u003c\/span\u003e\u003cspan\u003eDEPC-Water\u003c\/span\u003e\u003cspan\u003e to make up to 200\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eB. Lysis of virus-infected tissues\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e: \u003c\/span\u003e\u003cspan\u003eprepare \u003c\/span\u003e\u003cspan\u003e10 mg of virus-infected tissues to be ground with liquid nitrogen, and add 200\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el\u003c\/span\u003e\u003cspan\u003e of PBS solution or \u003c\/span\u003e\u003cspan\u003eDEPC-Water\u003c\/span\u003e\u003cspan\u003e to the ground tissues.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eC. Throat swab (with preservation solution)\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e: Vortex vigorously for 1 min, take 200ul for experiment.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eD. Throat swab (dry)\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e: Add 300\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eBuffer MVL\u003c\/span\u003e\u003cspan\u003e and 10\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el\u003c\/span\u003e\u003cspan\u003e Proteinase K, vortex vigorously for 15 sec, mix well. Incubate at 56\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e for 10 min, centrifuge at the highest speed for 1 min, take 200\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el\u003c\/span\u003e\u003cspan\u003e supernatant and add 2\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el\u003c\/span\u003e\u003cspan\u003e Carrier RNA. Continue the experiment from step 4.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. \u003c\/span\u003e\u003cspan\u003eAdd 200\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eBuffer MVL\u003c\/span\u003e\u003cspan\u003e, 10\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el\u003c\/span\u003e\u003cspan\u003e Proteinase K and 2\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el\u003c\/span\u003e\u003cspan\u003e Carrier RNA, vortex vigorously for 15 sec, mix well.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3. \u003c\/span\u003e\u003cspan\u003eIncubate at 56\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e for 10 min\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4. \u003c\/span\u003e\u003cspan\u003eAdd 400\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eBuffer MB and 25\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el \u003c\/span\u003e\u003cspan\u003eMagV Beads\u003c\/span\u003e\u003cspan\u003e to the above mixture\u003c\/span\u003e\u003cspan\u003e, mix well. Let stand for 3-5 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5. \u003c\/span\u003e\u003cspan\u003eTransfer the above mixture to the magnetic separation rack, \u003c\/span\u003e\u003cspan\u003eand let stand \u003c\/span\u003e\u003cspan\u003efor 3-5 minutes to absorb the beads. Carefully discard all solutions.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e6. \u003c\/span\u003e\u003cspan\u003eAdd 500\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el \u003c\/span\u003e\u003cspan\u003eBuffer MW1\u003c\/span\u003e\u003cspan\u003e. \u003c\/span\u003e\u003cspan\u003eVortex mix for 15 seconds\u003c\/span\u003e\u003cspan\u003e. Transfer the reclosion to the new tube.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e7\u003c\/span\u003e\u003cspan\u003e. \u003c\/span\u003e\u003cspan\u003eTransfer the above mixture to the magnetic separation rack, and \u003c\/span\u003e\u003cspan\u003elet stand\u003c\/span\u003e\u003cspan\u003e for 2 minutes to absorb the beads. Carefully discard all solutions.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e8. Add 600 ul 75% ethanol and vortex the mixture for 15 sec\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e9. Transfer the above mixture to the magnetic separation rack, and \u003c\/span\u003e\u003cspan\u003elet stand\u003c\/span\u003e\u003cspan\u003e for 2 minutes to absorb the beads. Carefully discard all solutions.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e10. \u003c\/span\u003e\u003cspan\u003eRepeat steps 7-9 once.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e11. \u003c\/span\u003e\u003cspan\u003eCentrifuge briefly to collect droplets from the wall of the tube.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eTransfer to the \u003c\/span\u003e\u003cspan\u003emagnetic separation rack\u003c\/span\u003e\u003cspan\u003e and carefully discard all solution\u003c\/span\u003e\u003cspan\u003es.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e12. Air drying for 7\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003e10 min.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e13. Add 30~50\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el \u003c\/span\u003e\u003cspan\u003eDEPC-Water\u003c\/span\u003e\u003cspan\u003e, vortex to disperse magnetic beads.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eLet stand for 3\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003e5 min, during which gently oscillate 1\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003e2 times to accelerate nucleic acid dissolution.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e14. Transfer to the magnetic \u003c\/span\u003e\u003cspan\u003eseparation rack\u003c\/span\u003e\u003cspan\u003e and let stand for 3 min.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eTransfer the nucleic acid solution to a new 1.5 ml centrifuge tube.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"100 preps","offer_id":39771534753892,"sku":"V4002-GB","price":214.65,"currency_code":"CAD","in_stock":true},{"title":"200 preps","offer_id":39771534786660,"sku":"V4002-200-GB","price":403.88,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/noimagedefaultimage-jpeg_87db6362-2456-4b7b-b6e4-61715abb9fff.jpg?v=1662136889"},{"product_id":"hipure-viral-rna-kit","title":"HiPure Viral RNA Kit","description":"\u003ch3\u003eIntroduction\u003c\/h3\u003e\n\u003cp\u003eHipure viral RNA kit is suitable for purifying viral RNA from samples such as cell-free body fluid or culture medium. The kit is based on silica gel column purification technology. It requires no toxic phenol chloroform extraction and time-consuming alcohol precipitation in the extraction. The whole extraction process takes only 25 minutes. The kit is suitable for extracting viral RNA from 1-140µl cell-free fluid samples such as serum, plasma, cell-free body fluid or culture medium. The product has successfully extracted hepatitis B A\/C, hepatitis C RNA, SARS and HIV. The obtained RNA can be directly used for RT-PCR, Northern hybridization and virus detection.\u003c\/p\u003e\n\u003ch3\u003eDetails\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"1185\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eFeatures\u003c\/td\u003e\n\u003ctd\u003eSpecifications\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Functions\u003c\/td\u003e\n\u003ctd\u003eExtract viral RNA from 140μl cell-free samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eApplications\u003c\/td\u003e\n\u003ctd\u003eRT-PCR, Northern hybridization, and various virus detection\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePurification method\u003c\/td\u003e\n\u003ctd\u003eMini spin column\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePurification technology\u003c\/td\u003e\n\u003ctd\u003eSilica technology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProcess method\u003c\/td\u003e\n\u003ctd\u003eManual (centrifugation or vacuum)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eCell-free body fluid or culture medium\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample amount\u003c\/td\u003e\n\u003ctd\u003e140μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eElution volume\u003c\/td\u003e\n\u003ctd\u003e≥15μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTime per run\u003c\/td\u003e\n\u003ctd\u003e≤25 minutes\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLiquid carrying volume per column\u003c\/td\u003e\n\u003ctd\u003e800μl\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBinding yield of column\u003c\/td\u003e\n\u003ctd\u003e100μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch1\u003e\n\u003cbr\u003ePrinciple\u003c\/h1\u003e\n\u003cp\u003eHipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.\u003c\/p\u003e\n\u003cp\u003eThe sample is homogenized and lysed in the lysate, and RNA is released into the lysate. The high concentration of guanidine isothiocyanate contained in the lysate denatured and inactivated endogenous or exogenous RNase, while RNA is protected from degradation. The lysate is centrifuged to remove insoluble impurities. After adding ethanol to adjust the binding conditions, it is transferred to the column for filtration. RNA is adsorbed on the membrane of the column, while protein is removed without adsorption. The column is washed with buffer VHB to remove protein and other impurities, washed with buffer RW2 to remove salt. Finally the RNA is eluted by RNase free water. The eluted RNA can be directly used in RT-PCR, Northern blot, poly-A purification, in vitro translation, etc.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAdvantages\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFast - several samples can be extracted in 20 minutes by column method\u003c\/li\u003e\n\u003cli\u003eHigh quality - high purity total RNA can be directly used in various sensitive downstream applications\u003c\/li\u003e\n\u003cli\u003eSafe - no phenol chloroform extraction required\u003c\/li\u003e\n\u003cli\u003eSensitive - RNA can be recovered at the level of PG\u003c\/li\u003e\n\u003cli\u003eHigh yield - carrier RNA contained in the product maximize the recovery of trace nucleic acid\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Contents\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"1185\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eContents\u003c\/td\u003e\n\u003ctd\u003eR417102\u003c\/td\u003e\n\u003ctd\u003eR417103\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePurification Times\u003c\/td\u003e\n\u003ctd\u003e50 Preps\u003c\/td\u003e\n\u003ctd\u003e250 Preps\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHiPure Viral Micro Columns\u003c\/td\u003e\n\u003ctd\u003e50\u003c\/td\u003e\n\u003ctd\u003e250\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2ml Collection Tubes\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e100\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer VRL\u003c\/td\u003e\n\u003ctd\u003e50 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e200 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCarrier RNA\u003c\/td\u003e\n\u003ctd\u003e310 µg\u003c\/td\u003e\n\u003ctd\u003e3 x 310 µg\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer VHB*\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e13 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e110 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer RW2*\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e20\u003cspan\u003e \u003c\/span\u003eml\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e2 x 50 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNuclease Free Water\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e10 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e30 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage and Stability\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eCarrier RNA should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under theseconditions\u003c\/p\u003e\n\u003ch3\u003eArticles\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan style=\"text-decoration: underline;\" data-mce-style=\"text-decoration: underline;\"\u003e\u003ca href=\"https:\/\/journals.asm.org\/doi\/abs\/10.1128\/JVI.00667-20\" data-mce-href=\"https:\/\/journals.asm.org\/doi\/abs\/10.1128\/JVI.00667-20\"\u003e【JOURNAL OF VIROLOGY】L226Q Mutation on Influenza H7N9 Virus Hemagglutinin Increases Receptor-Binding Avidity and Leads to Biased Antigenicity Evaluation | Journal of Virology\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"text-decoration: underline;\" data-mce-style=\"text-decoration: underline;\"\u003e\u003ca href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2095809918309366\" data-mce-href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2095809918309366\"\u003e【Engineering】Redesigned Duplex RT-qPCR for the Detection of GI and GII Human Noroviruses\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"text-decoration: underline;\" data-mce-style=\"text-decoration: underline;\"\u003e\u003ca href=\"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2021.673872\/full\" data-mce-href=\"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2021.673872\/full\"\u003e【Frontiers in Microbiology】Development of a High-Efficiency Immunomagnetic Enrichment Method for Detection of Human Norovirus via PAMAM Dendrimer\/SA-Biotin Mediated Cascade-Amplification\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"text-decoration: underline;\" data-mce-style=\"text-decoration: underline;\"\u003e\u003ca href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0956713521002991\" data-mce-href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0956713521002991\"\u003e【FOOD CONTROL】Detection of group A rotavirus in oyster tissues by in situ capture RT-qPCR\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"text-decoration: underline;\" data-mce-style=\"text-decoration: underline;\"\u003e\u003ca href=\"https:\/\/www.mdpi.com\/950828\" data-mce-href=\"https:\/\/www.mdpi.com\/950828\"\u003e【Viruses-Basel】Library Preparation Based on Transposase Assisted RNA\/DNA Hybrid Co-Tagmentation for Next-Generation Sequencing of Human Noroviruses\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"text-decoration: underline;\" data-mce-style=\"text-decoration: underline;\"\u003e\u003ca href=\"https:\/\/onlinelibrary.wiley.com\/doi\/abs\/10.1002\/jmv.28216\" data-mce-href=\"https:\/\/onlinelibrary.wiley.com\/doi\/abs\/10.1002\/jmv.28216\"\u003e【JOURNAL OF MEDICAL VIROLOGY】GII.17[P17] and GII.8[P8] noroviruses showed different RdRp activities associated with their epidemic characteristics\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\u003cbr\u003e\u003c\/h3\u003e","brand":"GeneBio Systems","offers":[{"title":"50 preps","offer_id":41740388040804,"sku":"R417102","price":204.42,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/hipureviralrnakitimage.png?v=1683470041"}],"url":"https:\/\/www.genebiosystems.com\/en-us\/collections\/viral-rna-isolation-kits.oembed","provider":"GeneBio ","version":"1.0","type":"link"}