{"title":"RNA Isolation and DNA Isolation","description":"\u003cp\u003e\u003ca title=\"In the RNA Isolation Kits category, we have the following isolation kits: Micro RNA (miRNA): MicroRNAs are small RNA molecules as short as 18 nucleotides. They require special protocols to be isolated.  General RNA Isolation: RNA is not as stable as DNA and thus, there are special protocols for RNA isolation for each sample types.  Fixed\/embedded Tissue: In order to perform PCR of targets from these samples, isolation of nucleic acids is critical.  \u0026lt;Blood: A common sample type, it requires special protocol for RNA isolation.  Plant: Plant samples contain high level of inhibitors to PCR and other reactions, thus require a specific protocols for RNA isolation.  Tissue\/Cells: Tissues and cells are rich in ribonucleases, optimized RNA protocols and reagents are provided in our products.\" href=\"https:\/\/www.genebiosystems.com\/collections\/general-rna-isolation\" style=\"font-size: 0.875rem;\"\u003e\u003cspan\u003e\u003cspan style=\"color: #000000; text-decoration: underline;\"\u003e\u003cspan\u003eRNA Isolation\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e \u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\" data-mce-fragment=\"1\"\u003eUncover a diverse range of RNA isolation solutions tailored to your specific needs:\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eUniversal RNA Kits (with chloroform):\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eVersatile kits designed for comprehensive RNA extraction across various sample types.\u003c\/li\u003e\n\u003cli\u003eUtilizes chloroform to ensure efficient isolation and purification of RNA molecules.\u003c\/li\u003e\n\u003cli\u003eIdeal for a wide range of applications, offering flexibility in experimental design.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTotal RNA Kits (chloroform-free):\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eInnovative kits engineered for total RNA extraction without the use of chloroform.\u003c\/li\u003e\n\u003cli\u003eEnsures a chloroform-free process for enhanced user safety and convenience.\u003c\/li\u003e\n\u003cli\u003eSuitable for applications demanding high-purity RNA, including downstream analyses like qPCR and RNA sequencing.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eMicrobial, Cell, and Plant Kits:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSpecialized kits addressing the unique challenges of RNA isolation from microbial, cell, and plant samples.\u003c\/li\u003e\n\u003cli\u003eOptimized protocols for efficient RNA extraction while preserving sample integrity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTRAzol Reagents:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eCutting-edge TRAzol reagents for RNA, DNA, and protein isolation from a single sample.\u003c\/li\u003e\n\u003cli\u003eStreamlines and simplifies the extraction process, saving time and resources.\u003c\/li\u003e\n\u003cli\u003eIdeal for applications where comprehensive nucleic acid and protein analysis is required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003ca title=\"In the DNA isolation categories, we have isolation kits for the following subcategories: Apoptotic DNA Ladder: Observed often on apoptotic cells, DNA ladders can be isolated\/analyzed for your samples using the kits.  Bacterial\/yeast Plasmid: For use in preparing plasmid from bacteria or yeast, in mini, midi, maxi prep scales.  Bacterial\/yeast Genomic: Genomic DNA from bacteria or yeast can be isolated using these kits.  Blood\/Tissue\/Cells: These kits are suitable for isolating genomic DNA from blood, tissue and cells.  Plants\/Marine Animal: For high quality DNA isolation from plant samples or marine animal tissues.  M13\/Lambda Phage: You can use M13 and Lambda phage DNA using these kits.  Stool\/Urine: Specially optimized for isolating DNA from stool or urine samples.  Saliva\/Swab: For use in preparing DNA from saliva or swab samples.  Fixed and Embedded Tissue: these kits can be used to isolate DNA from fixed\/embedded FFPE samples.  Viral DNA: These products can be used to isolate DNA from viruses in blood and other bodily fluids, or supernatent of cultures.\" href=\"https:\/\/www.genebiosystems.com\/12-dna-isolation\" style=\"font-size: 0.875rem;\"\u003e\u003cspan style=\"color: #000000; text-decoration: underline;\"\u003eDNA Isolation\u003c\/span\u003e\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDNA Isolation:\u003c\/strong\u003e Explore our extensive range of DNA isolation kits, each designed to address specific sample types and research needs:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eGenomic DNA Extraction Kits:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eTailored for the extraction of high-quality genomic DNA from a variety of sources.\u003c\/li\u003e\n\u003cli\u003eEnsures purity and integrity for downstream applications such as PCR, sequencing, and genotyping.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eViral DNA Extraction Kits:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSpecialized kits for efficient isolation of viral DNA from diverse sample matrices.\u003c\/li\u003e\n\u003cli\u003eIdeal for virology research and diagnostic applications requiring viral DNA analysis.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTissue DNA Extraction Kits:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eRobust kits optimized for the extraction of DNA from various tissue types.\u003c\/li\u003e\n\u003cli\u003eEnables high-yield and high-quality DNA isolation for molecular biology applications.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBacterial DNA Extraction Kits:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDesigned for efficient and reliable bacterial DNA extraction from different bacterial species.\u003c\/li\u003e\n\u003cli\u003eStreamlined protocols for quick and convenient isolation suitable for microbiology studies.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eStool, Urine, Saliva Sample Kits, and More:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eComprehensive kits catering to specific sample types, including stool, urine, saliva, and more.\u003c\/li\u003e\n\u003cli\u003eEnsures compatibility with a wide range of sample matrices for diverse research applications.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ca title=\"In this category, Other molecular Reagents, we have the following subcategories (product groups): DNA Digestion: DNAses used often in preparation of RNA and protein.  RNA Digestion: RNases, used often in preparation of DNA from various samples and reaction mixtures.  PCR Cross-contamination: UDG and dUTP can be used to systematically remove carry-over contamination in PCR. In Vitro Transcription: Reagent and kits used to generate RNA from DNA templates.\" href=\"https:\/\/www.genebiosystems.com\/14-digestion-enzymes\" style=\"font-size: 0.875rem;\"\u003e\u003cspan style=\"color: #000000; text-decoration: underline;\"\u003eDigestion Enzymes\u003c\/span\u003e\u003c\/a\u003e\u003cbr\u003e\u003cspan style=\"color: #000000; text-decoration-line: underline; font-size: 0.875rem;\"\u003eand more\u003c\/span\u003e\u003cbr\u003e\n\u003cul class=\"dynamized\"\u003e\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eDNA Isolation\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/DNA_Isolation.pdf?v=1684864472\"\u003e Brochure\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eRNA Isolation \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/RNA_Isolation.pdf?v=1684864470\"\u003eBrochure\u003c\/a\u003e\u003c\/p\u003e","products":[{"product_id":"easyspin-plant-mirna-kit","title":"EASYspin Plant miRNA kit","description":"\u003cp\u003eEASYspin Plant miRNA kit\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"50 Preps","offer_id":39752492974180,"sku":"RNK4001-01","price":395.05,"currency_code":"CAD","in_stock":true},{"title":"100 Preps","offer_id":39752493006948,"sku":"RNK4001-02","price":768.14,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/172.jpg?v=1643252325"},{"product_id":"plexprep-4-rna-dna-mirna-protein-extract-kit","title":"PlexPrep-4 RNA-DNA-miRNA-protein Extract Kit","description":"\u003cp\u003eThe Kit is used to extract and collect RNA,DNA,miRNA,protein separately from blood. Kits of 50 or 100 preps are available.\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"50 preps","offer_id":39766537732196,"sku":"RNK4701-01","price":493.81,"currency_code":"CAD","in_stock":true},{"title":"100 preps","offer_id":39766537764964,"sku":"RNK4701-02","price":932.75,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/222.jpg?v=1643677149"},{"product_id":"benzonase-nuclease-250-u-ul","title":"Benzonase Nuclease, 250U\/µl","description":"\u003cp\u003e\u003cspan\u003eBenzonase Nuclease, No-specific endonuclease, 250 U\/µl, is a genetically engineered endonuclease from \u003cem\u003eSerratia marcescens\u003c\/em\u003e. Benzonase® Nuclease. It is a highly useful endonuclease for removal of nucleic acids DNA and RNA, from recombinant proteins and protein fragments, and is also used for the reduction of viscosity in protein extracts.\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e The enzyme completely digests nucleic acids to 5'-monophosphate terminated oligonucleotides 2–5 bases in length. Although the nuclease is capable of cleavage at nearly all positions along a nucleic acid chain, sequence-dependent preferences have been demonstrated. The enzyme prefers GC-rich regions in dsDNA while avoiding d(A)\/d(T)-tracts. Benzonase is ideal for a wide variety of applications where complete digestion of nucleic acids is desirable. Digests native or heat-denatured DNA and RNA. This can also be used for the removal of nucleic acid from protein samples.\u003cbr\u003e\u003cbr\u003e Unit definition:\u003cbr\u003e One unit of Benzonase Nuclease is defined as the amount of enzyme that causes a ΔA260 of 1.0 in 30 min, which corresponds to complete digestion of 37 μg of DNA. Note that 1 KU = 1000 units. \u003cbr\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e• It possesses a higher specific activity than other nucleases\u003c\/p\u003e\n\u003cp\u003e• It is free of detectable proteolytic activities\u003c\/p\u003e\n\u003cp\u003e• It can be used effectively in various fields of applications, including applications in biotechnological processing, such as recombinant protein expression and purification, and biopharmaceutical production • It has a proven track record in various commercial vaccine manufacturing processes. \u003cstrong\u003eBulk quantities are available. GMP grade is also available. See our GMP section for more information. \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/GBS-Benzonase_User_Manual-Copy.pdf?v=1705431907\"\u003e\u003cstrong\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cimg data-mce-fragment=\"1\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"153\" height=\"43\" data-mce-selected=\"1\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\"\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"5000U","offer_id":39766539305060,"sku":"OTD-01","price":54.87,"currency_code":"CAD","in_stock":true},{"title":"25,000U","offer_id":39766539337828,"sku":"OTD-02","price":252.39,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/noimagedefaultimage-jpeg_79486e0d-7800-4dc4-9560-d3b37d870074.jpg?v=1647396538"},{"product_id":"proteinase-k-lyophilized","title":"Proteinase K  Lyophilized Powder","description":"\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eProteinase K, lyophilized, Specific activity ≥30 U\/mg protein, is a broad substrate non-specific serine proteinase. It is very stable at pH 4-12 and can be used in isolating mRNA, genomic DNA and digesting unwanted proteins during DNA and RNA preparations from tissues and cells. It’s also been used on glycoprotein modification and protein structure studies. Proteinase K is active with SDS, urea and EDTA. the very agents that inactivates nucleases.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eProteinase K is used for the isolation of native high molecular genomic nucleic acids. Enzymes like DNases and RNases from microorganisms and mammalian cells are rapidly inactivated by Proteinase K.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSpecific Activity:\u003c\/strong\u003e \u003cstrong\u003e\u003cspan style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003e≥3\u003c\/span\u003e0 U\/mg protein\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSource: \u003c\/strong\u003eTritirachium album limber\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMolecular Weight:\u003c\/strong\u003e 29,730\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCAS: \u003c\/strong\u003e39450-01-6\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEINECS No:\u003c\/strong\u003e3.4.21.64\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePurity:\u003cspan style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003e≥\u003c\/span\u003e\u003c\/strong\u003e95%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm:\u003c\/strong\u003e Lyophilized powder. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eStorage Conditions:\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e Store vial at 2-4ºC upon receipt.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eDissolve and aliquot and store at -20ºC recommended.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eUse sterile filtration for liquid enzyme storage if keep it at 2-8ºC over a week.\u003c\/p\u003e\n\u003cp\u003eStorage Buffer: 20 mM Tris-HCl (pH 7.4), 1 mM CaCl2, 50% Glycerol (Dilution buffer without glycerol). QC Assays: Unit Definition: One unit is defined as the amount of enzyme that will liberate 1 μmol of tyrosine per minute at 37ºC, pH7.5.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"http:\/\/www.mebep.com\/a\/Products\/Enzyme\/2013\/0823\/DNase%20I.html\" data-mce-href=\"http:\/\/www.mebep.com\/a\/Products\/Enzyme\/2013\/0823\/DNase%20I.html\"\u003eDNase\u003c\/a\u003e Activity: None detectable enzyme activity with λ DNA after 6 hrs incubation at 37ºC.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"http:\/\/www.mebep.com\/a\/Products\/Enzyme\/2013\/0823\/RNase%20A.html\" data-mce-href=\"http:\/\/www.mebep.com\/a\/Products\/Enzyme\/2013\/0823\/RNase%20A.html\"\u003eRNase\u003c\/a\u003e Activity: None detectable ribonuclease activity after 16 hrs incubation at 25ºC.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003eAdding Proteinase K already during the cell lysis enables the isolation of highly native undamaged high molecular DNA or RNA. A variety of methods have been established, which are documented in numerous publications.\u003cbr\u003e\u003cbr\u003eRecently, Proteinase K has been used for the detection of BSE forming proteins which are uniquely resistant towards the enzyme's proteolytic cleavage.\u003cbr\u003e\u003cbr\u003eProteinase K is very useful in the analysis of membrane structure by means of modification of proteins and glycoproteins on cell surfaces.\u003cbr\u003e\u003cbr\u003eBecause of the cleavage specificity Proteinase K, characteristic fragments of proteins are obtained which are helpful in revealing the structure and function of proteins, particularly enzymes.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/Proteinase_K-N9016-Lyophilized_User_Manual.pdf?v=1709225452\" data-mce-href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/Proteinase_K-N9016-Lyophilized_User_Manual.pdf?v=1709225452\"\u003e\u003cspan\u003e\u003cspan style=\"text-decoration: underline;\" data-mce-style=\"text-decoration: underline;\"\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\" width=\"185\" height=\"52\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"100 mg","offer_id":39766542712932,"sku":"N9016","price":44.55,"currency_code":"CAD","in_stock":true},{"title":"1 g","offer_id":39766542745700,"sku":"N9017","price":229.68,"currency_code":"CAD","in_stock":true},{"title":"5 g","offer_id":39766542778468,"sku":"N9018","price":613.75,"currency_code":"CAD","in_stock":true},{"title":"20 g","offer_id":41950700339300,"sku":"N9019","price":2621.4,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/269.jpg?v=1643677242"},{"product_id":"rnase-a-powder","title":"RNase A-powder","description":"\u003cp\u003ePancreatic Ribonuclease (RNase  A ) catalyzes cleavage of the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide forming a 2',3'-cyclic phosphate which may then be hydrolyzed to the corresponding 3'-nucleoside phosphate. Ribonuclease A has a molecular weight of 13,700 daltons. It operates in an optimum pH range of 7.0-7.5. Activity \u003cspan class=\"font6\"\u003e≥\u003c\/span\u003e\u003cspan class=\"font5\"\u003e 50 KU\/mg peotein, Lyophilized Powder\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eSource: \u003c\/em\u003e\u003cem\u003eBovine Pancrease EC:3.1.27.5\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003eThe ultrapure Pancreatic Ribonuclease has been further purified by affinity chromatography method to remove trace contaminating proteases.\u003c\/p\u003e\n\u003cp\u003eForm: lyophilized powder\u003c\/p\u003e\n\u003cp\u003eSPECIFICATION (I)\u003c\/p\u003e\n\u003cp\u003eActivity ≥ 50 Kunitz units\/mg powder\u003c\/p\u003e\n\u003cp\u003eSTORAGE Store in an airtight container, at a temperature of 2°C to 8°C.\u003c\/p\u003e\n\u003ch3\u003e\u003c\/h3\u003e","brand":"Gene Bio Systems","offers":[{"title":"100 mg","offer_id":39766545039460,"sku":"N9046","price":61.67,"currency_code":"CAD","in_stock":true},{"title":"1 g","offer_id":39766545072228,"sku":"N9047","price":479.71,"currency_code":"CAD","in_stock":true},{"title":"5 g","offer_id":39766545104996,"sku":"N9047b","price":2063.02,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/249.jpg?v=1643677301"},{"product_id":"plexprep-3-rna-dna-mirna-extract-kit","title":"PlexPrep-3 RNA-DNA-miRNA Extract Kit","description":"\u003cp\u003eThe Kit is used to extract and collect RNA,DNA and miRNA separately from blood. Kits of 50 or 100 preps are available.\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"50 preps","offer_id":39766545203300,"sku":"RNK4801","price":438.94,"currency_code":"CAD","in_stock":true},{"title":"100 preps","offer_id":39766545236068,"sku":"RNK4801","price":768.14,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/250.jpg?v=1643677306"},{"product_id":"mieasy-kit-for-paxgene-tube","title":"miEASY Kit (for PAXgene tube)","description":"\u003cdiv id=\"divDescription\"\u003e\n\u003cdiv id=\"productHead\" class=\"productHead\"\u003e\n\u003cp\u003eFor purification of miRNA and total RNA from whole blood\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"divFeatures\"\u003e\n\u003cdiv id=\"productFeatures\" class=\"dottedList\"\u003e\n\u003cul\u003e\n\u003cli\u003eEffective purification of miRNA and total RNA\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"divShortinfo\"\u003e\n\u003cdiv id=\"productShortInfo\" class=\"copy\"\u003e\n\u003cp\u003eThe PAXgene Blood miRNA System consists of the PAXgene Blood miRNA Kit for purification of total RNA, including RNA longer than approximately 18 nucleotides. Purification is carried out using silica-based RNA purification technology in a spin-column format. Purification can be carried out using a microcentrifuge. Used with the tubes, the kit provides a complete pre-analytical solution for collection and stabilization through to purification of high-quality total RNA, including miRNA, for molecular analysis.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"50 preps","offer_id":39766545662052,"sku":"RNK0501","price":263.37,"currency_code":"CAD","in_stock":true},{"title":"100 preps","offer_id":39766545694820,"sku":"RNK0501","price":482.83,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/251.jpg?v=1643677320"},{"product_id":"e-coli-uracil-dna-glycosylase-udg","title":"E. coli Uracil-DNA Glycosylase (UDG)","description":"\u003cp\u003e\u003cem\u003eE. coli\u003c\/em\u003e Uracil-DNA Glycosylase (UDG) is a recombinant enzyme purified from an \u003cem\u003eE. coli\u003c\/em\u003e strain that carries the UDG gene from \u003cem\u003eE. coli\u003c\/em\u003e.\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"5000 U","offer_id":39766547431524,"sku":"OTP-01","price":197.52,"currency_code":"CAD","in_stock":true},{"title":"25,000 U","offer_id":39766547464292,"sku":"OTP-01","price":877.88,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_309d961b-e467-4d9b-90a9-fc0f0f735868.jpg?v=1706882257"},{"product_id":"gb-clean-plasmid-miniprep-kit","title":"GB-Clean™ Plasmid Miniprep Kit (HiPure Mini system)","description":"\u003ch2\u003e\n\u003cspan style=\"font-size: 0.875rem;\"\u003eDetails\u003c\/span\u003e\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe GB-Clean™ Plasmid Miniprep Kit (HiPure Mini system) provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. GB-Clean™ Plasmid Miniprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of tris buffer or water.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable width=\"1185\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eFeatures\u003c\/td\u003e\n\u003ctd\u003eSpecifications\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Functions\u003c\/td\u003e\n\u003ctd\u003eIsolation up to 35μg plasmid DNA from 1-5ml bacterial culture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eApplications\u003c\/td\u003e\n\u003ctd\u003eEnzyme digestion, sequencing, PCR, cloning, etc.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePurification method\u003c\/td\u003e\n\u003ctd\u003eMini spin column\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePurification technology\u003c\/td\u003e\n\u003ctd\u003eSilica technology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProcess method\u003c\/td\u003e\n\u003ctd\u003eManual (centrifugation or vacuum)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eConventional plasmid, plasmid less than 30KB\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample amount\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eHigh copy plasmid: 1-5ml culture medium\u003c\/p\u003e\n\u003cp\u003eLow copy number plasmid : 5-10ml culture medium\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eYield\u003c\/td\u003e\n\u003ctd\u003e5-35µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eElution volume\u003c\/td\u003e\n\u003ctd\u003e≥30μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTime per run\u003c\/td\u003e\n\u003ctd\u003eComplete 1-24 samples in 30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLiquid carrying volume per column\u003c\/td\u003e\n\u003ctd\u003e800µl\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBinding yield of column\u003c\/td\u003e\n\u003ctd\u003e35µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch1\u003e\n\u003cbr\u003ePrinciple\u003c\/h1\u003e\n\u003cp\u003eThe HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method, then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).\u003c\/p\u003e\n\u003cp\u003eSee manual for more details on P1001:\u003c\/p\u003e\n\u003cp\u003e\u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/P1001_GBS-Manual_GB-Clean-Plasmid-Miniprep-Kit_Mar-2025_3c80698a-616e-483a-9356-c222c26eb340.pdf?v=1744374106\" target=\"_blank\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\" width=\"188\" height=\"53\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAdvantages\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eHigh purity - purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.\u003c\/li\u003e\n\u003cli\u003eFast - it takes only 1 minute to obtain supernatant by optimized solution (10 minutes for other brands)\u003c\/li\u003e\n\u003cli\u003eHigh yield (see comparison below)- up to 35µg plasmid can be bound in one column\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eKit Contents\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ctable style=\"height: 209px;\" width=\"1185\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 319.177px;\"\u003eContents\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 98.9062px;\"\u003eP100101\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 112.583px;\"\u003eP100103\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 319.177px;\"\u003ePurification Times\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 98.9062px;\"\u003e50 Preps\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 112.583px;\"\u003e250 Preps\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 319.177px;\"\u003eRNase A\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 98.9062px;\"\u003e2 mg\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 112.583px;\"\u003e10 mg\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 319.177px;\"\u003eBuffer P1\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 98.9062px;\"\u003e15 ml\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 112.583px;\"\u003e80 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 319.177px;\"\u003eBuffer P2\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 98.9062px;\"\u003e15 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 112.583px;\"\u003e80 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 319.177px;\"\u003eBuffer P3\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 98.9062px;\"\u003e20 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 112.583px;\"\u003e100 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 319.177px;\"\u003eBuffer PW1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 98.9062px;\"\u003e25 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 112.583px;\"\u003e140 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 319.177px;\"\u003eBuffer PW2*\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 98.9062px;\"\u003e10 ml\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 112.583px;\"\u003e50 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 319.177px;\"\u003eElution Buffer\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 98.9062px;\"\u003e6 ml\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 112.583px;\"\u003e30  ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 319.177px;\"\u003eHiPure DNA Mini Columns II\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 98.9062px;\"\u003e50\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 112.583px;\"\u003e250\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 319.177px;\"\u003e2 ml Collection Tubes\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 98.9062px;\"\u003e50\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 112.583px;\"\u003e250\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eStorage and Stability\u003c\/h2\u003e\n\u003cp\u003eThe kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃\u003cspan\u003e \u003c\/span\u003eto dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C.\u003c\/p\u003e\n\u003ch2\u003eExperiment Data\u003c\/h2\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/experimentaldatafigureforminiprepmagen.png?v=1681153639\" width=\"1026\" height=\"426\"\u003e\u003c\/p\u003e\n\u003cp\u003eSee also the attached data figure.\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/P1001_HiPure_Plasmid_DNA_Mini_Kit-GBS_1.pdf?v=1700841634\"\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"50 Preps","offer_id":39766548119652,"sku":"P100101","price":74.94,"currency_code":"CAD","in_stock":true},{"title":"250 Preps","offer_id":39766548185188,"sku":"P100103","price":349.27,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/p100101_image_3.jpg?v=1742213763"},{"product_id":"marine-animal-tissue-genomic-dna-kit","title":"Marine Animal Tissue Genomic DNA Kit","description":"\u003cp\u003e\u003cspan\u003eThe Marine Animal Tissue Genomic DNA Purification Kit provides a simple and rapid method for high quality genomic DNA purification from tissues(either fresh or frozen at -70\u003cspan\u003e°\u003c\/span\u003eC until use) of marine animal, such as fish, shrimp, shellfish or crab. The Genomic DNA system uses the silica-gel-membrane technology for simple and fast isolation of Genomic DNA without phenol\/chloroform. Homogenization is not necessary since tissues are directly lysed by Proteinase K. The buffer system is optimized to allow selective binding of DNA to the silica-gel membrane. The simple centrifugation protocol completely removes contaminants such as proteins, divalent cations, and secondary metabolites. Pure DNA is then eluted in water or low-salt buffer, ready to use. The typical yield of genomic DNA is 3-35 µg from 10 mg of tissue. The purified high molecular weight genomic DNA is suitable for direct use in all common molecular biology applications: PCR, restriction digestion, cloning, DNA sequencing and Southern blot analysis.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cbr\u003e Features\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cbr\u003e Efficient: 3-35 µg of genomic DNA from 10 mg of tissue or 1 x 106-107 cultrue cells. \u003cbr\u003e Fast: Procedure takes only 30 min. \u003cbr\u003e Universal: Purifies genomic DNA from various sources. \u003cbr\u003e Safe: No phenol\/cholroform extraction step. \u003cbr\u003e High purity: Purified DNA is ready for downstream application such as PCR, restriction digestion.\u003cbr\u003e\u003cbr\u003e Downstream Applications\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cbr\u003e Purified DNA is free from contaminants and enzyme inhibitors, and typically has A260\/A280 ratios between 1.7 and 1.9, and is suitable for applications such as:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eü\u003c\/span\u003e\u003cspan\u003e Restriction digestion\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eü\u003c\/span\u003e\u003cspan\u003e PCR\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eü\u003c\/span\u003e\u003cspan\u003e Labeling\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eü\u003c\/span\u003e\u003cspan\u003e Library construction\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eKit Content: (for a 50-prep kit size)\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eComponent \u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eVolume or Size\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eSolution DS\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e15 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eSolution MS\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e20 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eProteinase K\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eWash Buffer (PS)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e30 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eWash Buffer (PE)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e15 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eElution Buffer TE\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e 5 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eSpin Columns\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e50 each\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003cp\u003e\u003cspan\u003e\u003cbr\u003e\u003cbr\u003e Storage\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cbr\u003e Store Protein K at -20\u003cspan\u003e℃\u003c\/span\u003e, other reagents can be stored at room temperature for up to 1 year. Any precipitate in the Solution DS and Solution MS can be re-dissolved by incubating at 37\u003cspan\u003e°\u003c\/span\u003eC before use.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cbr\u003e Important Notes\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cbr\u003e\u003cspan\u003e•\u003c\/span\u003e Prior to the initial use of the kit, dilute the Wash Buffer(PE) with ethanol (96-100%):\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eN1172(100preps)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eWash Buffer(PE)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e30 ml x2\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eEthanol\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e, , 90 ml x2\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eTotal Volume\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e120 ml x2\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAfter the ethanol has been added, mark the check box on the bottle to indicate the completed step.\u003cbr\u003e\u003cspan\u003e•\u003c\/span\u003e Examine the solution for precipitates before each use. Re-dissolve any precipitate by warming the solution to 37\u003cspan\u003e°\u003c\/span\u003eC and cooling to 25\u003cspan\u003e°\u003c\/span\u003eC.\u003cbr\u003e\u003cspan\u003e•\u003c\/span\u003e All purification steps should be carried out at room temperature.\u003cbr\u003e\u003cbr\u003e Protocol\u003cbr\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1.Marine animal tissue (either fresh or frozen at -70\u003cspan\u003e°\u003c\/span\u003eC until use) can be processed by freezing with liquid nitrogen and grinding into a fine powder using a mortar and pestle. Add 10mg of this tissue powder to a 1.5ml microcentrifuge tube. Do not exceed 10 mg or it will reduce the lyse efficiency.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.Add 200 \u003cspan\u003eμ\u003c\/span\u003el Solution DS. Vortex vigorously to resuspend cells.It is essential that the sample and Solution DS are mixed immediately and thoroughly by vortexing or pipetting to yield a homogeneous solution. \u003cbr\u003e Optional If RNA-free genomic DNA is required, add 4 \u003cspan\u003eμ\u003c\/span\u003el RNase A (100 mg\/ml) and incubate for 5 minutes at room temperature. RNase A (100 mg\/ml) can be purchased separately .\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.Add 20 \u003cspan\u003eμ\u003c\/span\u003el Proteinase K. Mix thoroughly by vortexing. Incubate at 55\u003cspan\u003e℃\u003c\/span\u003e and inverting at times till a clear homogeneou solution is produced, spin down the water beads from the wall of the tube.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4.Add 220 \u003cspan\u003eμ\u003c\/span\u003el Solution MS, Mix thoroughly by vortexing. Incubate at 65\u003cspan\u003e°\u003c\/span\u003eC for 10 minutes to yield a homogeneous solution. Spin down the water beads from the wall of the tube.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5.Add 220 \u003cspan\u003eμ\u003c\/span\u003el ethanol (96\u003cspan\u003e–\u003c\/span\u003e100%) to the sample, and mix thoroughly by inverting. A precipitate may appear. Pipet the mixture from step 4 into the spin column placed in a 2 ml collection tube (provided). Centrifuge at 12,000rpm for 1 min. Discard flow-through.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e6.Add 500 \u003cspan\u003eμ\u003c\/span\u003el Wash Buffer PS, and centrifuge for 1 min at 12,000 rpm. Discard flow-through.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e7.Add 500 \u003cspan\u003eμ\u003c\/span\u003el Wash Buffer PE, and centrifuge for 1 min at 12,000 rpm. Discard flow-through. Repeat step 6 again.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e8.Centrifuge for 3 min at 12,000 rpm to dry the column membrane. Discard flow-through and collection tube. \u003cbr\u003e Note It is important to dry the membrane of the spin column, since residual ethanol may interfere with subsequent reactions. This centrifugation step ensures that no residual ethanol will be carried over during the following elution.Following the centrifugation step, remove the spin column carefully so that the column does not come into contact with the flow-through, since this will result in carryover of ethanol. If carryover of ethanol occurs, empty the collection tube, then reuse it in another centrifugation for 1 min at 12,000 rpm.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e9.Place the spin column in a clean 1.5 ml microcentrifuge tube (not provided), and pipet 30-100 \u003cspan\u003eμ\u003c\/span\u003el Eluent Buffer AE (prewarm to 65\u003cspan\u003e℃\u003c\/span\u003e)directly onto the membrane. Incubate at room temperature for 2 minutes, and then centrifuge for 2 min at 12,000 rpm to elute. The tube contains the purified DNA. Store the DNA at -20\u003cspan\u003e℃\u003c\/span\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"50 preps","offer_id":39766548676708,"sku":"N117-01","price":109.74,"currency_code":"CAD","in_stock":true},{"title":"100 preps","offer_id":39766548709476,"sku":"N117-02","price":208.5,"currency_code":"CAD","in_stock":true},{"title":"200 preps","offer_id":39766548742244,"sku":"N117-03","price":395.05,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/263.jpg?v=1643677387"},{"product_id":"mieasy-microrna-kit","title":"miEASY microRNA Kit","description":"\u003cp\u003emiEASY microRNA Blood\/Serum\/Plasma Kit is a Blood microRNA extraction kit. Similar to Qiagen miRNeasy* Serum\/Plasma \/whole blood Kit. In addition to Serum and plasma, our miEASY kit can also be used to isolate miRNA from whole blood.\u003c\/p\u003e\n\u003cp\u003eFor purification of cell-free total RNA, including miRNA, from animal and human plasma and serum\u003c\/p\u003e\n\u003cp\u003e• Internal control available for normalization\u003c\/p\u003e\n\u003cp\u003e• Minimal elution volume\u003c\/p\u003e\n\u003cp\u003e• High-purity RNA suitable for all downstream applications\u003c\/p\u003e\n\u003cp\u003e• Easy, robust procedures\u003c\/p\u003e\n\u003cp\u003e• Automatable protocol\u003c\/p\u003e\n\u003cp\u003eThe MiEasy Serum\/Plasma Kit is designed for purification of cell-free total RNA — primarily miRNA and other small RNA — from small volumes of serum and plasma. RNA from serum and plasma typically consists of molecules \u0026lt;100 nucleotides.\u003c\/p\u003e\n\u003cp\u003e*miRNeasy is a name of similar product of Qiagen\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"50 Preps","offer_id":39766549299300,"sku":"RNK4601-01","price":274.33,"currency_code":"CAD","in_stock":true},{"title":"100 Preps","offer_id":39766549332068,"sku":"RNK4601-02","price":526.74,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/266.jpg?v=1643677408"},{"product_id":"pax-blood-mirna-kit","title":"PAX Blood miRNA Kit","description":"\u003cp\u003ePAX Blood miRNA extraction Kit is used to extract miRNA form PAXgene blood tube. Kits of 50 preps are 100 preps are both available.\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"50 preps","offer_id":39766549561444,"sku":"RNK5101-01","price":438.94,"currency_code":"CAD","in_stock":true},{"title":"100 preps","offer_id":39766549594212,"sku":"RNK5101-02","price":768.14,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/271.jpg?v=1643677419"},{"product_id":"on-column-dnase-digestion-kit","title":"On-Column DNase Digestion Kit","description":"\u003cp\u003e\u003cstrong\u003e50rxns\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eDNase Digestion Kit (On Spin Columns) is for removing DNA on spin columns used during RNA isolation procedures. Although these procedures are capable of removing most DNA, small amount of DNA remains. For demanding applications where all DNA must be removed, on-column digestion can be performed. Same as Qiagen RNase free DNase set (Cat# 79254*).\u003c\/p\u003e\n\u003cp\u003eSee protocol details for RNI02 in manual:\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/RNK3401_RNI02_Protocol_ON_Column_DNase_Digestion-_GBS_April_2025.pdf?v=1743602031\" rel=\"noopener\" target=\"_blank\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"228\" height=\"64\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e*Note: this catalog number is that of a product of Qiagen.\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39766549659748,"sku":"RNI02","price":108.63,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_194ad8f7-7c81-4ae4-af8a-50100cafdca9.jpg?v=1757609201"},{"product_id":"hipure-total-rna-mini-kit","title":"HiPure Total RNA Mini Kit (RNA Isolation\/Extraction Kit)","description":"\u003cdiv\u003eIsolation \/ extraction of total RNA from a large number of mammalian tissues and cells and yeast. \u003cbr\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cul\u003e\n\u003cli\u003eProvides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100ug RNA.\u003c\/li\u003e\n\u003cli\u003eNo need for phenol\/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl are eliminated\u003c\/li\u003e\n\u003cli\u003eApplications: RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul class=\"list-paddingleft-2\"\u003e\n\u003cli\u003e\u003cspan\u003eHigh quality - high purity total RNA can be directly used in various sensitive downstream applications\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eFast - several samples can be extracted in 20 minutes by column method\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eSafe - no phenol chloroform extraction required\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eSensitive - RNA can be recovered at the level of picogram\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eStorage\/Stability: store kit dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions.\u003c\/p\u003e\n\u003cp\u003eSee protocol details in manual:\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/R4011_HiPure_Total_RNA_Kit-GBS-50_and_250_preps.pdf?v=1700842592\" title=\"HiPUre Total RNA Kit\"\u003e\u003cimg height=\"60\" width=\"213\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eKit Contents\u003c\/p\u003e\n\u003ctable width=\"250\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003e\u003cstrong\u003eProduct \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003eR401102\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003eR401103\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003ePreparation Times\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003e50\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003e250\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 53px;\"\u003e\n\u003ctd style=\"width: 458.667px; height: 53px;\"\u003e\n\u003cp\u003eHiPure RNA Mini Columns\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 53px;\"\u003e50\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 53px;\"\u003e250\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003e2 mL Collection Tubes\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003e50\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003e250\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003eRTL Lysis Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003e40 mL\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003e200 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003eRNA Binding Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003e15 mL\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003e75 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003eBuffer RW1\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003e40 mL\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003e200 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003e\n\u003cp\u003eBuffer RW2*\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003e20 mL\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003e2 x 50 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 458.667px;\"\u003eRNase Free Water\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px;\"\u003e6 mL\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px;\"\u003e30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eCitations:\u003c\/p\u003e\n\u003cp\u003eLouis L, Ramzan F, Martin DD, Olaitan AO.0.Reversion of heme-dependent metronidazole resistance in Clostridioides difficile. Microbiol Spectr0:e01787-25.https:\/\/doi.org\/10.1128\/spectrum.01787-25\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"50 Preps","offer_id":39766553100388,"sku":"R401102","price":237.5,"currency_code":"CAD","in_stock":true},{"title":"250 Preps","offer_id":39766553133156,"sku":"R401103","price":1065.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/20250328_151443.jpg?v=1743595623"},{"product_id":"t7-high-efficiency-transcription-kit","title":"T7 High Efficiency Transcription Kit","description":"\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eT7 High Efficiency Transcription Kit is designed for in vitro RNA synthesis by T7 RNA Polymerase with supercoiled or linearized DNA templates. Up to 150 μg of RNA can be produced from a 20 μl reaction. Synthesized RNA can be used for in vitro translation, RNase protection assays, RNA splicing, and hybridization assays.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eat -20°C for one year\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eKit Content\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003cp\u003e\u003c\/p\u003e\n\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eComponent\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eJT101-01 (25 rxns)\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eT7 Transcription Enzyme Mix\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e50 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e5×T7 Transcription Reaction Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e100 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e10 mM NTP Mix\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e200 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eDNase I (1 unit\/µl)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e25 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e500 mM EDTA (pH 8.0)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e25 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eRNase-free Water\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e500 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eControl Transcription Template (0.5 µg\/µl)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e10 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39766554837092,"sku":"JT101","price":342.92,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/314.jpg?v=1643677639"},{"product_id":"proteinase-k-solution","title":"Proteinase K Solution","description":"\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eProteinase K solution,20mg\/ml(\u0026gt;680U\/ml). Proteinase K is a broad substrate non-specific serine proteinase. It is very stable at pH 4-12 and can be used in isolating mRNA, genomic DNA and digesting unwanted proteins during DNA and RNA preparations from tissues and cells. It’s also been used on glycoprotein modification and protein structure studies. Proteinase K is active with SDS, urea and EDTA, the very agents that inactivates nucleases.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eProteinase K is used for the isolation of native high molecular genomic nucleic acids. Enzymes like DNases and RNases from microorganisms and mammalian cells are rapidly inactivated by Proteinase K.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSpecific Activity:\u003c\/strong\u003e \u003cstrong\u003e40 U\/mg protein\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSource: \u003c\/strong\u003eTritirachium album limber\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMolecular Weight:\u003c\/strong\u003e 29,730\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCAS: \u003c\/strong\u003e39450-01-6\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEINECS No:\u003c\/strong\u003e3.4.21.64\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePurfity:\u0026gt;\u003c\/strong\u003e99.9%\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Conditions:\u003c\/strong\u003e Storage at -20ºC is recommended.\u003c\/p\u003e\n\u003cp\u003eUse sterile filtration for liquid enzyme storage if keep it at 2-8ºC over a week.\u003c\/p\u003e\n\u003cp\u003eStorage Buffer: 20 mM Tris-HCl (pH 7.4), 1 mM CaCl2, 50% Glycerol (Dilution buffer without glycerol). QC Assays: Unit Definition: One unit is defined as the amount of enzyme that will liberate 1 μmol of tyrosine per minute at 37ºC, pH7.5.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"http:\/\/www.mebep.com\/a\/Products\/Enzyme\/2013\/0823\/DNase%20I.html\"\u003eDNase\u003c\/a\u003e Activity: None detectable enzyme activity with λ DNA after 6 hrs incubation at 37ºC.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"http:\/\/www.mebep.com\/a\/Products\/Enzyme\/2013\/0823\/RNase%20A.html\"\u003eRNase\u003c\/a\u003e Activity: None detectable ribonuclease activity after 16 hrs incubation at 25ºC.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003eAdding Proteinase K already during the cell lysis enables the isolation of highly native undamaged high molecular DNA or RNA. A variety of methods have been established, which are documented in numerous publications.\u003cbr\u003e\u003cbr\u003eRecently, Proteinase K has been used for the detection of BSE forming proteins which are uniquely resistant towards the enzyme's proteolytic cleavage.\u003cbr\u003e\u003cbr\u003eProteinase K is very useful in the analysis of membrane structure by means of modification of proteins and glycoproteins on cell surfaces.\u003cbr\u003e\u003cbr\u003eBecause of the cleavage specificity Proteinase K, characteristic fragments of proteins are obtained which are helpful in revealing the structure and function of proteins, particularly enzymes.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/Proteinase_K-_N9011_-Solution1-gbs.pdf?v=1700836787\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"213\" height=\"60\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"1 mL","offer_id":39766558015588,"sku":"N9011","price":26.67,"currency_code":"CAD","in_stock":true},{"title":"5 mL","offer_id":39766558048356,"sku":"N9012","price":106.69,"currency_code":"CAD","in_stock":true},{"title":"50 mL","offer_id":41950614454372,"sku":"N9013","price":647.74,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/332.jpg?v=1643677725"},{"product_id":"fixed-and-embedded-tissue-mirna-kit","title":"Fixed and Embedded Tissue miRNA kit","description":"\u003cdiv id=\"divDescription\"\u003e\n\u003cdiv id=\"productHead\" class=\"productHead\"\u003e\n\u003cp\u003eThe Easyspin RNAExtration Kit(same as Qiagen RNeasy* Mini Kit 74104) offers a fast and easy way to extract high quality RNA from tissues and cells. The whole process is phenol\/chloroform-free. The unique lysis buffer immediately lyses biological samples and inactivates RNase and DNase. Ethanol is added to the lysate to provide appropriate binding conditions for RNA, and RNA selectively binds to the silica-membrane of the RNA column in the high-salt buffer. RNA is purified through a series of wash-spin steps to remove protein followed by elution of RNA from silica membrane with RNase-free H2O.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFeatures:\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e\u003cbr\u003e 1. Fast procedure delivering high-quality total RNA in minutes \u003cbr\u003e 2. Ready-to-use RNA for high performance in any downstream application \u003cbr\u003e 3. Consistent RNA yields from small amounts of starting material\u003cbr\u003e 4. High-performance RNA for sensitive applications\u003cbr\u003e 5. No phenol\/chloroform extraction, no CsCl gradients, no LiCl or ethanol precipitation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e*Qiagen RNeasy is a name used by Qiagen for similar products.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Gene Bio Systems","offers":[{"title":"50 preps","offer_id":39766558343268,"sku":"RNI-06-01","price":457.23,"currency_code":"CAD","in_stock":true},{"title":"100 preps","offer_id":39766558376036,"sku":"RNI-06-02","price":889.06,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/334.jpg?v=1643677734"},{"product_id":"trazol-rna-reagent","title":"TRA-zol RNA Isolation Reagent","description":"\u003cp\u003eTRA-zol Reagent is an improved version of the popular single-step total RNA isolation reagent developed by Chomczynski and Sacchi (1987). The RNA isolation (extraction) method based on this single reagent is widely used and proven for RNA applications. It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA, and proteins from samples of human, animal, plant, yeast, bacterial, and viral origin.\u003c\/p\u003e\n\u003cp\u003eTRA-zol Reagent performs well with easily scalable small or large amounts of tissue or cells, and many samples can be simultaneously extracted.\u003c\/p\u003e\n\u003cp\u003eThis product, a mixture of guanidine thiocyanate and phenol in a monophase solution, effectively dissolves DNA, RNA, and protein upon homogenization or lysis of tissue sample. After adding chloroform or 1-bromo-3-chloropropane and centrifuging, the mixture separates into 3 phases: an aqueous phase containing the RNA, the interphase containing DNA, and an organic phase containing proteins. Each component can then be isolated after separating the phases. One milliliter of TRAzol is sufficient to isolate RNA, DNA, and protein from 50-100 mg of tissue, 5-10 million cells, or 10 cm² of culture dish surface for cells grown in monolayer.\u003c\/p\u003e\n\u003cp\u003eThis is one of the most effective methods for isolating total RNA and can be completed in only 1 hour starting with fresh tissue or cells. The procedure is very effective for isolating RNA molecules of all types from 0.1-15 kb in length. The resulting RNA is intact with little or no contaminating DNA and protein.\u003c\/p\u003e\n\u003cp\u003eThe RNA extraction reagent has been widely used for isolating RNA, similar to the leading RNA Isolation Reagents. Resulting RNA can be used for Northern blots, mRNA isolation, in vitro translation, RNase protection assay, cloning, and PCR.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFeatures and Benefits:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e• Easily scalable RNA isolation • Works with many sources: human, plant, yeast, bacterial, or viral • Better yields than traditional guanidine thiocyanate\/cesium chloride methods • Cost-effective compared to other similar single-reagent RNA isolation reagents\u003c\/p\u003e\n\u003ch4\u003ePackaging\u003c\/h4\u003e\n\u003cp\u003eChoose 100, 200, 500  or 1000mL sizes. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/Trazol_Magzol_Reagent_User_Manual.pdf?v=1688853244\" title=\"Protocol for Trazol Magzol\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"221\" height=\"62\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eChomczynski, P. and Sacchi, N. Single-step method of RNA isolation by acid guanidinium thiocyanatephenol-chloroform extraction. Anal. Biochem. 162: 156-159. 1987.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Gene Bio Systems","offers":[{"title":"200mL","offer_id":39766558703716,"sku":"R1022b","price":579.4,"currency_code":"CAD","in_stock":true},{"title":"400mL","offer_id":42713521029220,"sku":"R1022c","price":966.72,"currency_code":"CAD","in_stock":true},{"title":"1.2L","offer_id":42713521061988,"sku":"R1022d","price":2611.28,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/TRAzol_20250314_105917.jpg?v=1742485128"},{"product_id":"trazol-rna-reagent-ls","title":"TRA-zol RNA Reagent LS (MagZol LS Reagent)","description":"\u003cp\u003e\u003cspan\u003e\u003cspan\u003eTRA-zol RNA Reagent LS is a complete, ready-to-use reagent optimized for the isolation of high-quality total RNA, or the simultaneous isolation of RNA, DNA, and protein from a variety of liquid samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from liquid samples of human, animal, plant, yeast, bacterial, and viral origin, typically within one hour. Key features of TRA-zol LS Reagent:\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Formulated for use with liquid samples such as serum and virus preparations\u003cbr\u003e\u003cspan\u003e• Facilitates recovery of RNA, DNA, and protein from a single liquid sample\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003e• Offers excellent lysis capability, even with difficult biological fluids\u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e\u003cspan\u003eReliably purify RNA from multiple sample sources\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e\u003cspan\u003eTRAzol LS Reagent is designed for processing a variety of liquid samples of up to 0.25 mL in volume. TRI-Zoe LS Reagent differs from the standard TRAzol LS Reagent in concentration, which permits larger samples to be processed. Just as with the standard TRAzol LS Reagent, the integrity of resulting RNA preparations is maintained by the highly effective inhibition of RNase activity during sample homogenization. The simplicity of the TRI-Zoe LS Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in 1 hour. Total RNA isolated by TRAzol LS Reagent is free of protein and DNA contamination.\u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e\u003cspan\u003eFormulated to serve multiple isolations\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e\u003cspan\u003eTRI-Zoe LS Reagent allows you to perform sequential precipitation of RNA, DNA, and proteins from a single sample. After homogenizing the sample with TRA-zol LS Reagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (containing RNA), an interface, and a red lower organic layer (containing the DNA and proteins). RNA is precipitated from the aqueous layer with isopropanol. DNA is precipitated from the aqueous\/organic interface with ethanol. Protein is precipitated from the phenol-ethanol layer by isopropanol precipitation. The precipitated RNA, DNA, or protein is washed to remove impurities, and then resuspended for use in downstream applications.\u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e\u003cspan\u003ePurified products are ideal for use with a variety of applications\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e\u003c\/span\u003eIsolated RNA can be used in real-time quantitative PCR (qPCR), northern blot analysis, dot blot hybridization, poly-(A)+ selection, In vitro translation, RNase protection assays, and molecular cloning. Isolated DNA can be used in PCR, restriction enzyme digestion, and Southern blots. Isolated protein can be used for western blots, recovery of some enzymatic activity, and some immunoprecipitation\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"100 ml","offer_id":39766558769252,"sku":"RNI-07-01","price":410.66,"currency_code":"CAD","in_stock":true},{"title":"200 ml","offer_id":39766558802020,"sku":"RNI-07-02","price":749.35,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/noimagedefaultimage-jpeg_ac6d5f28-6567-495f-8d68-789cd4d1e2c4.jpg?v=1653486590"},{"product_id":"rnakeeper-stabilization-buffer","title":"RNAkeeper Stabilization Buffer","description":"\u003cdiv class=\"text parbase section\"\u003e\n\u003cp\u003eRNA\u003cem\u003ekeeper\u003c\/em\u003e Stabilization Buffer is used for stabilizing RNA. It is an aqueous, nontoxic tissue storage reagent that rapidly permeates tissues to stabilize and protect cellular RNA. RNA\u003ci\u003ekeeper\u003c\/i\u003e Stabilization Buffer provides a convenient option for the user to stabilize RNA without processing the tissue\/sample right away or freezing them in liquid nitrogen.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"text parbase section\"\u003e\n\u003cp\u003eIt provides:\u003c\/p\u003e\n\u003cp\u003ea) Immediate RNase inactivation\u003c\/p\u003e\n\u003cp\u003eb) allows tissue or sample harvest\/collection any time\u003c\/p\u003e\n\u003cp\u003ec) no need to use liquid nitrogen\u003c\/p\u003e\n\u003cp\u003ed) allows isolation of intact RNA anytime using our RNA isolation kits.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Gene Bio Systems","offers":[{"title":"50 ml","offer_id":39766558900324,"sku":"RNI-08-01","price":63.5,"currency_code":"CAD","in_stock":true},{"title":"100 ml","offer_id":39766558933092,"sku":"RNI-08-02","price":114.31,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/noimagedefaultimage-jpeg_ff8fe699-67f1-4ccd-bbb2-a45e39735d78.jpg?v=1688950332"},{"product_id":"fixed-and-embedded-tissue-rna-kit","title":"Fixed and embedded tissue RNA Kit","description":"\u003cp\u003eDeveloped specifically for Fixed and embedded tissue, using the spin column format. This Fast RNA Kit is Phenol free, chloroform free. There is no need for DNAse digestion. Isolated RNA can be used directly for RT PCR and real time PCR. 50 preps per kit\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39766558965860,"sku":"RNI-09","price":97.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/335.jpg?v=1643677754"},{"product_id":"yeast-genomic-dna-extraction-kit","title":"Yeast Genomic DNA Extraction Kit","description":"\u003cp\u003eThe Yeast Genomic DNA Extraction Kit provides a simple and rapid method for high quality genomic DNA purification from yeast. The Yeast Genomic DNA system uses the silica-gel-membrane technology for simple and fast isolation of Genomic DNA without phenol\/chloroform. Homogenization is not necessary since tissues are directly lysed by Proteinase K. The buffer system is optimized to allow selective binding of DNA to the silica-gel membrane. The simple centrifugation protocol completely removes contaminants such as proteins, divalent cations, and secondary metabolites. Pure DNA is then eluted in water or low-salt buffer, ready to use. The typical yield of genomic DNA is 3-25 µg from 1–5 x 107 yeast cells. The purified high molecular weight genomic DNA is suitable for direct use in all common molecular biology applications: PCR, restriction digestion, cloning, DNA sequencing and Southern blot analysis.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003eFeatures\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003eEfficient: 3-35 µg of genomic DNA from 1 – 5 x 107 yeast cells. \u003cbr\u003eFast: Procedure takes only 1 h. \u003cbr\u003eSafe: No phenol\/chloroform extraction step. \u003cbr\u003eHigh purity: Purified DNA is ready for downstream application such as PCR, restriction digestion.\u003cbr\u003e\u003cbr\u003eDownstream Applications\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003ePurified DNA is free from contaminants and enzyme inhibitors, and typically has A260\/A280 ratios between 1.7 and 1.9, and is suitable for applications such as:\u003c\/p\u003e\n\u003cp\u003eü Restriction digestion\u003c\/p\u003e\n\u003cp\u003eü PCR\u003c\/p\u003e\n\u003cp\u003eü Labeling\u003c\/p\u003e\n\u003cp\u003eü Library construction\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003eStorage\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003eStore Protein K and Lyticase at -20℃, other reagents can be stored at room temperature for up to 1 year.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e• Prior to the initial use of the kit, dilute the Wash Buffer(PE) with \u003ca name=\"OLE_LINK24\"\u003e\u003c\/a\u003eethanol (96-100%):\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"160\" valign=\"top\"\u003e\n\u003cp\u003eWash Buffer(PE)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"145\" valign=\"top\"\u003e\n\u003cp\u003e15 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"155\" valign=\"top\"\u003e\n\u003cp\u003e30 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"160\" valign=\"top\"\u003e\n\u003cp\u003eAdd Ethanol\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"145\" valign=\"top\"\u003e\n\u003cp\u003e\u003cstrong\u003e45 ml\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"155\" valign=\"top\"\u003e\n\u003cp\u003e\u003cstrong\u003e90 ml\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"160\" valign=\"top\"\u003e\n\u003cp\u003e\u003cem\u003eTotal Volume\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"145\" valign=\"top\"\u003e\n\u003cp\u003e\u003cem\u003e60 ml\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"155\" valign=\"top\"\u003e\n\u003cp\u003e\u003cem\u003e120 ml\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eAfter the ethanol has been added, mark the check box on the bottle to indicate the completed step.\u003cbr\u003e• Examine the solution for precipitates before each use. Re-dissolve any precipitate by warming the solution to 37°C and cooling to 25°C.\u003cbr\u003e• All purification steps should be carried out at room temperature.\u003cbr\u003e\u003cbr\u003eProtocol\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e1.Add 1 -5 ml of an yeast culture (not exceed 5 x 107 cells) to a 1.5ml microcentrifuge tube. Centrifuge at 12,000 rpm for 12 min to pellet the cells. Remove the supernatant.\u003c\/p\u003e\n\u003cp\u003e2.Add 600 μl lyticase buffer and 200 U lyticase, mix thoroughly by vortexing, incubate at 30 ℃ for 30 minutes\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e3.Centrifuge at 5,000 rpm for 8–10 min to pellet the mixture. Romove the supernatant. Add 200 μl Solution DS to the pellet. It is essential that the sample and Solution DS are mixed immediately and thoroughly by vortexing or pipetting to yield a homogeneous solution. \u003cbr\u003eOptional If RNA-free genomic DNA is required, add 4 μl RNase A (100 mg\/ml) and incubate for 5 min at room temperature. RNase A (100 mg\/ml) can be purchased separately .\u003c\/p\u003e\n\u003cp\u003e4.Add 20 μl Proteinase K and 220 μl Solution MS. Mix thoroughly by vortexing. Incubate at 65°C for 10 minutes to yield a homogeneous solution. Spin down the water beads on the wall of the tube.\u003c\/p\u003e\n\u003cp\u003e5.Add 220 μl ethanol (96–100%) to the sample, and mix thoroughly by vortexing. It is important that the sample and the ethanol are mixed thoroughly. A precipitate may appear. Pipet the mixture from step 4 into the spin column placed in a 2 ml collection tube (provided). Centrifuge at 12,000rpm for 1 min. Discard flow-through.\u003c\/p\u003e\n\u003cp\u003e6.Add 500 μl Wash Buffer PS, and centrifuge for 1 min at 12,000 rpm.Discard flow-through.\u003c\/p\u003e\n\u003cp\u003e7.Add 500 μl Wash Buffer PE, and centrifuge for 1 min at 12,000 rpm. Discard flow-through. Repeat step 6 again.\u003c\/p\u003e\n\u003cp\u003e8.Centrifuge for 3 min at 12,000 rpm to dry the column membrane. Discard flow-through and collection tube. \u003cbr\u003eNote It is important to dry the membrane of the spin column, since residual ethanol may interfere with subsequent reactions. This centrifugation step ensures that no residual ethanol will be carried over during the following elution.Following the centrifugation step, remove the spin column carefully so that the column does not come into contact with the flow-through, since this will result in carryover of ethanol. If carryover of ethanol occurs, empty the collection tube, then reuse it in another centrifugation for 1 min at 12,000 rpm.\u003c\/p\u003e\n\u003cp\u003e9.Place the spin column in a clean 1.5 ml microcentrifuge tube (not provided), and pipet 30-100 μl Eluent Buffer AE (prewarm to 65℃)directly onto the membrane. Incubate at room temperature for 2 minutes, and then centrifuge for 2 min at 12,000 rpm to elute. The tube contains the purified DNA. Store the DNA at -20℃.\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"50 preps","offer_id":39766559228004,"sku":"N1161","price":167.65,"currency_code":"CAD","in_stock":true},{"title":"100 preps","offer_id":39766559260772,"sku":"N1162","price":321.33,"currency_code":"CAD","in_stock":true},{"title":"200 preps","offer_id":39766559293540,"sku":"N1163","price":447.07,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/341.jpg?v=1643677768"},{"product_id":"urine-dna-kit","title":"Urine DNA Kit","description":"\u003cp\u003eUrine DNA Quick Extraction Kit can be used to prepare DNA from urine for your biomarker or clinical studies. \u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39766560243812,"sku":"DNI-12-01","price":1371.69,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/350.jpg?v=1643677805"},{"product_id":"saliva-dna-collection-kit","title":"Saliva DNA Collection kit","description":"\u003cp\u003eSaliva DNA collection kit can be used to extract DNA present in saliva to conduct your biomarker or clinical studies. \u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"10 Preps","offer_id":40142072512612,"sku":"DNI-12","price":69.85,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/351.jpg?v=1643677810"},{"product_id":"saliva-dna-collection-and-extraction-kit","title":"Saliva DNA collection And Extraction kit","description":"\u003cp\u003eSaliva DNA collection and extraction kit can be used to collect saliva samples and extract DNA from the samples, for your clinical or biomarker studies. .\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"10 preps","offer_id":39766560538724,"sku":"DNI-13-01","price":95.26,"currency_code":"CAD","in_stock":true},{"title":"50 preps","offer_id":39766560571492,"sku":"DNI-13-02","price":345.46,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/352.jpg?v=1643677814"},{"product_id":"oral-pharynx-swab-fast-dna-kit","title":"Oral-Pharynx Swab Fast DNA Kit","description":"\u003cp\u003eOral\/Pharynx Swab Fast DNA Extraction Kit can be used to prepare DNA from oral swab samples in your clinical and biomarker discovery research. \u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39766560637028,"sku":"DNI-14","price":137.17,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/353.jpg?v=1643677818"},{"product_id":"rnase-a","title":"RNase A","description":"\u003cp\u003eThe RNase A (10 mg\/mL) is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. Also known as Ribonuclease A, it cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide. The resulting 2', 3'-cyclic phosphate is hydrolyzed to the corresponding 3'-nucleoside phosphate .\u003c\/p\u003e\n\u003cp\u003eApplications\u003c\/p\u003e\n\u003cp\u003e--\u003cspan\u003eDNase and protease-free \u003c\/span\u003e\u003cbr\u003e--Plasmid and genomic DNA preparation \u003cbr\u003e--Removal of RNA from recombinant protein preparations. \u003cbr\u003e--Ribonuclease protection assays\u003cbr\u003e--Mapping single-base mutations in DNA or RNA\u003c\/p\u003e\n\u003cp\u003eSource\u003cbr\u003eBovine pancreas. \u003cbr\u003e\u003cbr\u003eMolecular Weight\u003cbr\u003e13.7 kDa monomer.\u003cbr\u003e\u003cbr\u003eDefinition of Activity Unit\u003cbr\u003eOne unit of the enzyme causes an increase in absorbance of 1.0 at 260 nm when yeast RNA is hydrolyzed at 37°C and pH 5.0. \u003cbr\u003eFifty units are approximately equivalent to 1 Kunitz unit.\u003c\/p\u003e\n\u003cp\u003eStorage Buffer \u003cbr\u003eThe enzyme is supplied in: 50 mM Tris-HCl (pH 7.4) and 50% (v\/v) glycerol.\u003c\/p\u003e\n\u003cp\u003eQuality Control\u003cbr\u003eThe absence of endodeoxyribonucleases, exodeoxyribonucleases and proteases confirmed by appropriate quality tests. Functionally tested for RNA digestion in a plasmid DNA purification procedure.\u003cbr\u003e\u003cbr\u003eInhibition and Inactivation\u003cbr\u003e·Inhibitors: the most potent inhibitor is a ~50 kDa protein from cytosol of mammalian cells, e.g., RiboLock™ RNase Inhibitor.\u003cbr\u003e·Other inhibitors: uridine 2',3'-cyclic vanadate, 5'-diphosphoadenosine 3'-phophate and 5'-diphosphoadenosine 2'-phophate (2), SDS, diethyl pyrocarbonate, 4M guanidinium thyocyanate plus 0.1M 2-mercaptoethanol and heavy metal ions. Inactivated by phenol\/chloroform extraction. \u003cbr\u003e·Inactivated by phenol\/chloroform extraction.\u003cbr\u003e\u003cbr\u003eNote\u003cbr\u003e·The recommended working concentration for RNase A is 1-100 µg\/ml depending on the application. \u003cbr\u003e·The enzyme is active under a wide range of reaction conditions. At low salt concentrations (0 to 100 mM NaCl), RNase A cleaves single-stranded and double-stranded RNA as well the RNA strand in RNA-DNA hybrids. However, at NaCl concentrations of 0.3 M or higher, RNase A specifically cleaves single-stranded RNA.\u003c\/p\u003e\n\u003ch3\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/GBS_RNAse_A_N9041_User_Manual.docx?v=1700833800\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"178\" height=\"50\" data-mce-selected=\"1\"\u003e\u003c\/a\u003e\u003c\/h3\u003e","brand":"Gene Bio Systems","offers":[{"title":"1 ml","offer_id":39766562963556,"sku":"N9041-01","price":47.17,"currency_code":"CAD","in_stock":true},{"title":"5 ml","offer_id":39766562930788,"sku":"N9041-02","price":222.57,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/RnaseAN904110mgml.png?v=1682715656"},{"product_id":"transzol-up-plus-rna-kit","title":"TransZol Up Plus RNA Kit","description":"\u003ch5\u003e100 rxn\/kit\u003c\/h5\u003e\n\u003cp\u003e\u003cem\u003eTransZol\u003c\/em\u003e\u003cspan\u003e Up Plus RNA Kit is suitable for the isolation of total RNA from cells and tissues. \u003cem\u003eTransZol Up is a reagent that uses guanidine isothiocyanate to lyse cells and efficiently extract high-purity RNA from various tissues and cells while maintaining RNA integrity.\u003c\/em\u003e After the sample is lysed with \u003c\/span\u003e\u003cem\u003eTransZol \u003c\/em\u003e\u003cspan\u003eUp, the solution separates into an upper colorless aqueous phase (containing RNA), an interphase and a lower pink organic phase. A silica-based spin column is used to specifically bind to RNA in the aqueous phase. Compared with other total RNA extraction methods, \u003c\/span\u003e\u003cem\u003eTransZol \u003c\/em\u003e\u003cspan\u003eUp Plus RNA Kit possesses not only advantages of strong lysis capability, high yield and a wide range of applications of \u003c\/span\u003e\u003cem\u003eTransZol \u003c\/em\u003e\u003cspan\u003eUp\u003c\/span\u003e\u003cem\u003e,\u003c\/em\u003e\u003cspan\u003e but also the advantage of high purity of spin column extraction.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e Features:\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eStrong lysis capability: \u003c\/strong\u003eComplete and fast lysis, high yield.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRapid extraction:\u003c\/strong\u003e Completed in \u003cstrong\u003eone hour\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVisible workflow:\u003c\/strong\u003e Pink organic phase to facilitate separation from \u003cstrong\u003ecolorless aqueous phase\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh purity: \u003c\/strong\u003eMinimum DNA and protein contamination.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eSee protocol details in manual:\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/ER501_TransZol-Up-Plus-RNA-Kit_GBS-Manual_Jan-2025.pdf?v=1736864468\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e\u003cspan\u003e\u003cimg height=\"46\" width=\"164\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eSample Requirements:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eA wide range of applications enabled by the maximum binding capacity of the spin column of 100 μg: \u003cstrong\u003eanimal \u003c\/strong\u003eand \u003cstrong\u003eplant tissues\u003c\/strong\u003e,  \u003cstrong\u003eviruses\u003c\/strong\u003e, \u003cstrong\u003ebacteria\u003c\/strong\u003e or other samples.\u003c\/p\u003e\n\u003cp\u003eSmall amount of samples (50-100 mg tissues, 5×10^6 cells, or 200 μl blood) and large amount of samples (≥1g tissues or ≥10^7 cells).\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eTransZol Up Reagent at 4°C in dark; other components at room temperature (15°C- 25°C) \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/ER501_TransZol-Up-Plus-RNA-Kit_GBS-Manual_Jan-2025.pdf?v=1736864468\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ch3\u003eKit Content:\u003c\/h3\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ctable style=\"width: 547px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 296.852px;\"\u003e\n\u003cp\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 237.148px;\"\u003e\n\u003cp\u003e\u003cspan\u003eER501-01 (100 rxns)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 296.852px;\"\u003e\n\u003cp\u003e\u003cem\u003eTransZol \u003c\/em\u003e\u003cspan\u003eUp\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 237.148px;\"\u003e\n\u003cp\u003e\u003cspan\u003e100 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 296.852px;\"\u003e\n\u003cp\u003e\u003cspan\u003eClean Buffer 9 (CB9)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 237.148px;\"\u003e\n\u003cp\u003e\u003cspan\u003e110 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 296.852px;\"\u003e\n\u003cp\u003e\u003cspan\u003eWash Buffer 9 (WB9)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 237.148px;\"\u003e\n\u003cp\u003e\u003cspan\u003e24 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 296.852px;\"\u003e\n\u003cp\u003e\u003cspan\u003eRNase-free Water\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 237.148px;\"\u003e\n\u003cp\u003e\u003cspan\u003e40 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 296.852px;\"\u003e\n\u003cp\u003e\u003cspan\u003eRNA Spin Column with Collection Tubes\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 237.148px;\"\u003e\n\u003cp\u003e\u003cspan\u003e100 each\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 296.852px;\"\u003e\n\u003cp\u003e\u003cspan\u003eRNase-free Tube (1.5 ml)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 237.148px;\"\u003e\n\u003cp\u003e\u003cspan\u003e100 each\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLiterature Citing This Product.\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"453\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003ctd width=\"175\"\u003e\n\u003cp\u003e\u003cstrong\u003eLiterature\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"94\"\u003e\n\u003cp\u003e\u003cstrong\u003eJournal\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\n\u003cp\u003e\u003cstrong\u003eAuthor\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"73\"\u003e\n\u003cp\u003e\u003cstrong\u003eLink\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"175\"\u003e\n\u003cp\u003eThe AtrbohF-dependent regulation of ROS signaling is required for melatonin-induced salinity tolerance in Arabidopsis\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"94\"\u003e\n\u003cp\u003efree radical biology and medicine\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\n\u003cp\u003eZiping Chen,et al. 2017\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"73\"\u003e\n\u003cp\u003e\u003ca href=\"http:\/\/sci-hub.tw\/10.1016\/j.freeradbiomed.2017.04.009\"\u003eThe link\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"175\"\u003e\n\u003cp\u003eA systematic analysis of the role of GGDEF-EAL domain proteins in virulence and motility in Xanthomonas oryzae pv. oryzicola\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"94\"\u003e\n\u003cp\u003eScientific Reports\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\n\u003cp\u003eWei C, et al. 2016\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"73\"\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27053282\"\u003eThe link\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"175\"\u003e\n\u003cp\u003eMuscovy duck reovirus σNS protein triggersautophagy enhancing virus replication\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"94\"\u003e\n\u003cp\u003eVirology Journal\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\n\u003cp\u003eYijian Wu,et al. 2017\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"73\"\u003e\n\u003cp\u003e\u003ca href=\"http:\/\/sci-hub.tw\/10.1186\/s12985-017-0722-8\"\u003eThe link\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"175\"\u003e\n\u003cp\u003eThe Persimmon 9-lipoxygenase Gene DkLOX3 Plays Positive Roles in Both Promoting Senescence and Enhancing Tolerance to Abiotic Stress\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"94\"\u003e\n\u003cp\u003eFRONTIERS IN PLANT SCIENCE\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\n\u003cp\u003eHou Y, et al. 2015\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"73\"\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26697033\"\u003eThe link\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"175\"\u003e\n\u003cp\u003eTranscriptome Sequencing Analysis Reveals a Difference in Monoterpene Biosynthesis between Scented Lilium 'Siberia' and Unscented Lilium 'Novano'\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"94\"\u003e\n\u003cp\u003eFRONTIERS IN PLANT SCIENCE\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\n\u003cp\u003eZenghui Hu,et al. 2017\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"73\"\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=Transcriptome+Sequencing+Analysis+Reveals+a+Difference+in+Monoterpene+Biosynthesis+between+Scented+Lilium+%E2%80%98Siberia%E2%80%99+and+Unscented+Lilium+%E2%80%98Novano%E2%80%99\"\u003eThe link\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39766611787876,"sku":"ER501","price":386.11,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/20250328_143935.jpg?v=1743596034"},{"product_id":"transzol-plant","title":"TransZol Plant","description":"\u003cp\u003eIt is also suitable for the isolation of total RNA from animal tissues like fat, connective tissues etc.\u003c\/p\u003e\n\u003cp\u003eCompared with other total RNA extraction methods, TransZol Up Plus RNA Kit provides powerful lysis and easy column based purification.  \u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e• Superior lysis capability and higher RNA yield.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• The whole procedure can be completed in one hour.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Pink solution for easy visualizing different phases.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Unique dissolving solution for long-term RNA storage.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eat room temperature for one year.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eKit Content\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\"\u003e\n\u003cp\u003e\u003cspan\u003eComponent\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"2\"\u003e\n\u003cp\u003e\u003cspan\u003eET121-01\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eTP I buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e100 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eTP II buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e100 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eRNA Dissolving Solution\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e15 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLiterature Citing This Product\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"layui-table\"\u003e\n\u003ccolgroup\u003e\n\u003ccol width=\"55%\"\u003e\n\u003ccol width=\"10%\"\u003e\n\u003ccol width=\"5%\"\u003e\n\u003ccol width=\"14%\"\u003e\n\u003ccol width=\"8%\"\u003e\n\u003ccol width=\"8%\"\u003e\n\u003c\/colgroup\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eLiterature\u003c\/th\u003e\n\u003cth\u003eJournal\u003c\/th\u003e\n\u003cth\u003eIF\u003c\/th\u003e\n\u003cth\u003eAuthor\u003c\/th\u003e\n\u003cth\u003eDate\u003c\/th\u003e\n\u003cth\u003eLink\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eThe REL3-mediated TAS3 ta-siRNA pathway integrates auxin and ethylene signaling to regulate nodulation in Lotus japonicus\u003c\/td\u003e\n\u003ctd\u003eNew Phytologist\u003c\/td\u003e\n\u003ctd\u003e6.736\u003c\/td\u003e\n\u003ctd\u003eXiaolin Li, et al. National Key Laboratory of Plan\u003c\/td\u003e\n\u003ctd\u003e2014 Jan\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=The+REL3-mediated+TAS3+ta-siRNA+pathway+integrates+auxin+and+ethylene+signaling+to+regulate+nodulation+in+Lotus+japonicus\"\u003eThe original link\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePhytosulfokine Is Involved in Positive Regulation of Lotus japonicus Nodulation\u003c\/td\u003e\n\u003ctd\u003eMol plant-Microbe Interact\u003c\/td\u003e\n\u003ctd\u003e4.5\u003c\/td\u003e\n\u003ctd\u003eChao Wang, et al.\u003c\/td\u003e\n\u003ctd\u003e2015 Mar\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=Phytosulfokine+Is+Involved+in+Positive+Regulation+of+Lotus+japonicus+Nodulation\"\u003eThe original link\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePhosphoproteomic Analysis of Paper Mulberry Reveals Phosphorylation Functions in Chilling Tolerance\u003c\/td\u003e\n\u003ctd\u003eJ Proteome Res\u003c\/td\u003e\n\u003ctd\u003e4.2453\u003c\/td\u003e\n\u003ctd\u003eZhi Pi, et al.\u003c\/td\u003e\n\u003ctd\u003e2017 Mar\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=Phosphoproteomic+Analysis+of+Paper+Mulberry+Reveals+Phosphorylation+Functions+in+Chilling+Tolerance\"\u003eThe original link\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntegration of a Decrescent Transcriptome and Metabolomics Dataset of Peucedanum praeruptorum to Investigate the CYP450 and MDR Genes Involved in Coumarins Biosynthesis and Transport\u003c\/td\u003e\n\u003ctd\u003eFrontiers in Plant Science\u003c\/td\u003e\n\u003ctd\u003e3.94\u003c\/td\u003e\n\u003ctd\u003eYucheng Zhao, et al.\u003c\/td\u003e\n\u003ctd\u003e2015 Nov\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=Integration+of+a+Decrescent+Transcriptome+and+Metabolomics+Dataset+of+Peucedanum+praeruptorum+to+Investigate+the+CYP450+and+MDR+Genes+Involved+in+Coumarins+Biosynthesis+and+Transport\"\u003eThe original link\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTranscriptomic basis of functional difference and coordination between seeds and the silique wall of Brassica napus during the seed-filling stage\u003c\/td\u003e\n\u003ctd\u003ePlant Science\u003c\/td\u003e\n\u003ctd\u003e3.607\u003c\/td\u003e\n\u003ctd\u003eHan Liu, et al.\u003c\/td\u003e\n\u003ctd\u003e2015 Jan\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed?term=transcriptome%20basis%20of%20functional%20difference%20and%20coordination%20between%20seeds%20and%20the%20silique%20wall%20of%20brassica%20napus%20during%20the%20seed-filling%20stage\u0026amp;cmd=correctspelling\"\u003eThe original link\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39766611820644,"sku":"ET121","price":213.37,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/402.jpg?v=1643678075"},{"product_id":"proteinext-mammalian-total-protein-extraction-kit","title":"ProteinExt™ Mammalian Total Protein Extraction Kit","description":"\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eat -20°C for one year\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eKit Content\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003cp\u003e\u003c\/p\u003e\n\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eComponent\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eDE101-01\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eTotal Protein Extraction Buffer (TPEB)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e100 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cem\u003eProteinSafe\u003c\/em\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003cem\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e\u003cspan\u003eProtease Inhibitor Cocktail, EDTA-free (100×)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39771524005988,"sku":"DE101","price":323.64,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/noimagedefaultimage-jpeg_4926a9d0-6528-4304-928b-69a61193a36e.jpg?v=1661914211"},{"product_id":"proteinext-r-mammalian-mitochondria-isolation-kit-for-cultured-cells","title":"ProteinExt® Mammalian Mitochondria Isolation Kit for Cultured Cells","description":"\u003cp\u003eThis kit provides two options for the separation of mitochondria from cytosolic components: a reagent-based method or homogenization-based method. Reagent-based method uses a mild procedure to process single or multiple samples.\u003c\/p\u003e\n\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cem\u003eProtein\u003c\/em\u003e\u003c\/span\u003e\u003c\/span\u003eSafe\u003cspan\u003eTM\u003c\/span\u003e Protease Inhibitor Cocktail and MSB at -20°C , others at 2-8°C for one year\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eKit Content\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003cp\u003e\u003c\/p\u003e\n\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eComponent\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eDE401-01 (50 rxns)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eMitochondria Isolation Buffer I (MIB I)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e50 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eMitochondria Isolation Buffer II (MIB II)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e500 μl\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eMitochondria Isolation Buffer III (MIB III)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e65 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eMitochondria Storage Buffer (MSB)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eProteinSafe\u003cspan\u003eTM\u003c\/span\u003e Protease Inhibitor Cocktail, EDTA-free (100×)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1.2 ml\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39771524235364,"sku":"DE401","price":432.77,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_b117c8e0-7c07-44f1-b8e1-dd402986556e.jpg?v=1689954726"},{"product_id":"proteinext-r-mammalian-mitochondria-isolation-kit-for-tissue","title":"ProteinExt® Mammalian Mitochondria Isolation Kit for Tissue","description":"\u003cp\u003eThis kit provides two options for the separation of mitochondria from cytosolic components: a reagent-based method or homogenization-based method. Reagent-based method uses a mild procedure to process single or multiple samples.\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cem\u003eProteinSafe\u003c\/em\u003e\u003cspan\u003e™ Protease Inhibitor Cocktail and MSB at -20°C , others at 2-8°C for one year\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eKit Content\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003cp\u003e\u003c\/p\u003e\n\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eComponent\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eDE501-01 (50 rxns)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eMitochondria Isolation Buffer I (MIB I)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e50 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eMitochondria Isolation Buffer II (MIB II)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e500 μl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eMitochondria Isolation Buffer III (MIB III)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e65 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eMitochondria Storage Buffer (MSB)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e4 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eBovine Serum Albumin (BSA)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e500 mg\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cem\u003eProteinSafe\u003c\/em\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003cem\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e\u003cspan\u003eProtease Inhibitor Cocktail, EDTA-free (100×)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1.2 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39771524268132,"sku":"DE501","price":432.77,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_21b8bd86-0951-4ddb-80fe-79f5db094992.jpg?v=1689954824"},{"product_id":"96-deep-well-plate-viral-rna-dna-miniprep-kit-magnetic-beads","title":"96 Deep-Well Plate Viral RNA-DNA Miniprep Kit (Magnetic Beads)","description":"\u003cp\u003e\u003cstrong\u003eSize\u003c\/strong\u003e: 96 preps per kit.\u003c\/p\u003e\n\u003cp\u003eThe kit can be integrated with an automatic nucleic acid extractor, such as KingFisher,  for high-throughput extraction experiments, or manually operated with a magnetic separation rack. \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe purification system \u003c\/span\u003e\u003cspan\u003euses\u003c\/span\u003e\u003cspan\u003e superparamagnetic nano-magnetic particles as the matrix, which can adsorb nucleic acids specifically through hydrogen bonds and static electricity under the condition of high concentration of leachate, while \u003c\/span\u003e\u003cspan\u003eproteins and \u003c\/span\u003e\u003cspan\u003eother non-specific impurities are removed by washing\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eF\u003c\/span\u003e\u003cspan\u003einally \u003c\/span\u003e\u003cspan\u003ethe\u003c\/span\u003e\u003cspan\u003e nucleic acid are \u003c\/span\u003e\u003cspan\u003eeluted with low salt buffer or RNase\u003c\/span\u003e\u003cspan\u003e-f\u003c\/span\u003e\u003cspan\u003eree ddH\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003eO.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eThe purified nucleic acid can be used for various routine operations, including \u003c\/span\u003e\u003cspan\u003eRT\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003ePCR\u003c\/span\u003e\u003cspan\u003e, \u003c\/span\u003e\u003cspan\u003eRT\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003eqPCR\u003c\/span\u003e\u003cspan\u003e, fluorescence quantitative PCR\u003c\/span\u003e\u003cspan\u003e (qPCR)\u003c\/span\u003e\u003cspan\u003e and other downstream experiments.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eMain components\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe kit consists of the following components:\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable cellspacing=\"0\" width=\"314\" style=\"height: 231px;\"\u003e\n\u003ctbody\u003e\n\u003ctr class=\"firstRow\"\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eComponent\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eSize\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003ePlate-A (Lysis Buffer\/Mag Beads)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003e622\u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el\/well\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003ePlate-B (Wash Buffer)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003e600\u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el\/well\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003ePlate-C (Wash Buffer)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003e600\u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el\/\u003c\/span\u003e\u003cspan\u003ewell\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003ePlate-D (Elution Buffer)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003e50\u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el\/well\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eProteinase K\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003e1mL \u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003e 2\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eM\u003c\/span\u003e\u003cspan\u003eagnet\u003c\/span\u003e\u003cspan\u003eic\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eHead\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003e1 pc\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan\u003ePrepare 1× PBS solution, pH 7.4, in case of need.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eStorage conditions\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe kit can be stored for 12 months at room temperature (15-25\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e) under dry conditions.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eNotes\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e. Avoid repeated freezing\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003ethawing of samples, otherwise the extracted viral RNA will be degraded and the extracted amount will decrease.\u003c\/span\u003e\u003cspan\u003e The samples can be extracted immediately or stored at 4\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e for testing for no more than 24 hours. Long-term storage can be placed at -20\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e or -80\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e. All operating procedures, if not specified, are carried out at room temperature (15-25\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e. When using this kit, please wear lab coat, disposable latex gloves, disposable masks and use RNase-free consumables to avoid RNase pollution to the greatest extent.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ePrepare your own \u003c\/span\u003e\u003cspan\u003eRN\u003c\/span\u003e\u003cspan\u003ease-free pipette tips, etc.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e. Please read the instructions carefully before use and follow the instructions strictly.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eClinical samples should be carried out in the ultra clean table or biosafety cabinet.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5. \u003c\/span\u003e\u003cspan\u003eThere may be residual magnetic beads during elution\u003c\/span\u003e\u003cspan\u003e, magnetic beads should be avoided as far as possible when pipetting samples.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e6. \u003c\/span\u003e\u003cspan\u003eThe virus has a strong ability to infect, a variety of defense measures must be done before the operation.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eProper disposal of samples and reagent materials, thorough cleaning and disinfection of the operating table.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eSample preparation\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eA. \u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eThroat swab (with preservation solution), saliva\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e: vortex vigorously for 30 sec, take 200 ul for experiment.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eB. Plasma, serum and viral stock solution\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e: prepare 10-200\u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el of plasma, serum or viral stock solution, if the initial amount is less than 200 \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el, use PBS solution to make up to 200 \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eC. \u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eVirus-infected tissue\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e: prepare 10mg of virus-infected tissues to be ground with liquid nitrogen, and add 200\u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el of PBS solution to the ground tissues.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eProtocol\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003e: Automatic Operation Process of 96 Deep-Well Plate\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1. Take out the pre-packaged 96 deep-well plates.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. \u003c\/span\u003e\u003cspan\u003eO\u003c\/span\u003e\u003cspan\u003escillate \u003c\/span\u003e\u003cspan\u003efour\u003c\/span\u003e\u003cspan\u003e angles of \u003c\/span\u003e\u003cspan\u003ethe P\u003c\/span\u003e\u003cspan\u003elate\u003c\/span\u003e\u003cspan\u003e-A\u003c\/span\u003e\u003cspan\u003e to make the \u003c\/span\u003e\u003cspan\u003eMag Beads\u003c\/span\u003e\u003cspan\u003e suspended\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3. \u003c\/span\u003e\u003cspan\u003eOpen the sealing membranes of the 96 deep-well plates.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4.\u003c\/span\u003e\u003cspan\u003e Add 20 \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el Proteinase K and 200\u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el sample to each well of the P\u003c\/span\u003e\u003cspan\u003elate\u003c\/span\u003e\u003cspan\u003e-A.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5. Place the \u003c\/span\u003e\u003cspan\u003eM\u003c\/span\u003e\u003cspan\u003eagnet\u003c\/span\u003e\u003cspan\u003eic\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eHead\u003c\/span\u003e\u003cspan\u003e in\u003c\/span\u003e\u003cspan\u003eto\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ethe P\u003c\/span\u003e\u003cspan\u003elate\u003c\/span\u003e\u003cspan\u003e-C.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e6. Follow the instructions to put the 4 pre-packaged \u003c\/span\u003e\u003cspan\u003eplate\u003c\/span\u003e\u003cspan\u003es into the correct position of the machine\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e7\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eRun the vir\u003c\/span\u003e\u003cspan\u003eal nucleic acid \u003c\/span\u003e\u003cspan\u003eextraction program of magnetic bead method\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e“\u003c\/span\u003e\u003cspan\u003egdsbio_normal_96.bdz\u003c\/span\u003e\u003cspan\u003e”\u003c\/span\u003e\u003cspan\u003e, or refer to the \u003c\/span\u003e\u003cspan\u003e“\u003c\/span\u003e\u003cspan\u003eSheet of Program Design\u003c\/span\u003e\u003cspan\u003e”\u003c\/span\u003e\u003cspan\u003e below.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e8. At the end of the automation process, in the Plate-D is the DNA\/RNA solution, sealed with a sealing membrane and stored at -20\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e or -80\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eSheet of Program Design\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr class=\"firstRow\"\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003ePlate\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eOperation Process\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eA\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eM\u003c\/span\u003e\u003cspan\u003eixing at 20~55\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e for 10 min \u003c\/span\u003e\u003cspan\u003eby vibrating, and t\u003c\/span\u003e\u003cspan\u003ehe digested sample release DNA\/RNA to the \u003c\/span\u003e\u003cspan\u003eMag Beads\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003e  Incubate\u003c\/span\u003e\u003cspan\u003e for 2 min.\u003c\/span\u003e\u003cspan\u003eTansfer\u003c\/span\u003e\u003cspan\u003e the \u003c\/span\u003e\u003cspan\u003ebeads\u003c\/span\u003e\u003cspan\u003e to \u003c\/span\u003e\u003cspan\u003ePlate-\u003c\/span\u003e\u003cspan\u003eB.\u003c\/span\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eB\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eWash the beads by vibrating for 2 min, then tansfer\u003c\/span\u003e\u003cspan\u003e the \u003c\/span\u003e\u003cspan\u003ebeads to Plate-C.\u003c\/span\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eC\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eWash the beads by vibrating for 2 min, dry the beads for 2 min, then tansfer\u003c\/span\u003e\u003cspan\u003e the \u003c\/span\u003e\u003cspan\u003ebeads to Plate-D.\u003c\/span\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eD\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eElute the beads by vibrating for 2 min, then tansfer\u003c\/span\u003e\u003cspan\u003e the \u003c\/span\u003e\u003cspan\u003ebeads back to Plate-C.\u003c\/span\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eSuitable Instruments\u003cbr\u003eThermo KingFisher Flex96,\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39771531149412,"sku":"V4003","price":328.96,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/noimagedefaultimage-jpeg_ec7cc5a3-748c-4fc4-a8cc-8ddc565fb538.jpg?v=1647396457"},{"product_id":"mipure-cell-tissue-mirna-kit","title":"MiPure Cell-Tissue miRNA Kit","description":"\u003cp\u003eThis kit combines high-efficiency RNA Isolater extraction reagents and silica gel column purification technology to complete the extraction of miRNA within 1 hour; it can also be used to separate and extract large molecular RNA (\u0026gt;200 nt) or total RNA (including miRNA) to satisfy users Different needs.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eThe miRNA extraction efficiency is high, and the starting amount is wide;\u003cbr\u003eLarger RNA and genomic DNA have fewer residues;\u003cbr\u003eEasy to operate, strong compatibility\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eBox 1: Store at 4℃ and avoid light\u003cbr\u003eBox 2: Store at room temperature\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39771531444324,"sku":"RC201","price":836.99,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/noimagedefaultimage-jpeg_9c126986-70fa-47b8-bfa8-a92aa01e77e6.jpg?v=1661915401"},{"product_id":"viral-dna-rna-extraction-kit-spin-column-based","title":"Viral DNA-RNA Extraction Kit -Spin Column-based","description":"\u003cp\u003e\u003cstrong\u003e\u003cspan\u003e100\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003epreps\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe Viral DNA\/RNA Extraction Kit (Spin Column) is suitable for rapid extraction of high purity viral nucleic acid from plasma, serum, nasopharyngeal swab, sputum, bronchus\/alveolar lavage fluid, saliva, ascites, culture cell supernatant and urine. This kit is based on the silica gel column purification method. The sample is homogenized in the lysis buffer and the nucleic acid is released into the buffer. The \u003c\/span\u003e\u003cspan\u003elysis buffer\u003c\/span\u003e\u003cspan\u003e contains a high concentration of guanidine. In this condition, the membrane absorbs nucleic acid by hydrogen bond and electrostatic physical and chemical action, while proteins and other impurities are not absorbed. The lysate is transferred to the adsorption column for filtration, and the nucleic acid filter membrane is washed to remove residual proteins and other impurities, and finally eluted by the low-salt buffer solution. The obtained nucleic acids can be directly used in downstream related experiments such as reverse transcription, PCR, RT-PCR, fluorescence quantitative PCR, second-generation sequencing and Northern hybridization.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eMain components\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe kit consists of the following components:\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable cellspacing=\"0\" style=\"width: 546px;\"\u003e\n\u003ctbody\u003e\n\u003ctr class=\"firstRow\"\u003e\n\u003ctd valign=\"top\" style=\"width: 115.375px;\"\u003e\n\u003cp\u003e\u003cspan\u003eThe name of the reagent\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 113.625px;\"\u003e\n\u003cp\u003e\u003cspan\u003eAmount\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 300px;\"\u003e\n\u003cp\u003e\u003cspan\u003eC\u003c\/span\u003e\u003cspan\u003eomponent description\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" style=\"width: 115.375px;\"\u003e\n\u003cp\u003e\u003cspan\u003eLysis Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 113.625px;\"\u003e\n\u003cp\u003e\u003cspan\u003e50 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 300px;\"\u003e\n\u003cp\u003e\u003cspan\u003eProvide environment for \u003c\/span\u003e\u003cspan\u003elysing\u003c\/span\u003e\u003cspan\u003e and \u003c\/span\u003e\u003cspan\u003ebinding to the\u003c\/span\u003e\u003cspan\u003e column\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" style=\"width: 115.375px;\"\u003e\n\u003cp\u003e\u003cspan\u003eWash Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 113.625px;\"\u003e\n\u003cp\u003e\u003cspan\u003e24\u003c\/span\u003e\u003cspan\u003e ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 300px;\"\u003e\n\u003cp\u003e\u003cspan\u003eRemove residual proteins and other impurities\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" style=\"width: 115.375px;\"\u003e\n\u003cp\u003e\u003cspan\u003eElute Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 113.625px;\"\u003e\n\u003cp\u003e\u003cspan\u003e6 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 300px;\"\u003e\n\u003cp\u003e\u003cspan\u003eNuclease\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003efree solution\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" style=\"width: 115.375px;\"\u003e\n\u003cp\u003e\u003cspan\u003eSpin Column\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 113.625px;\"\u003e\n\u003cp\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003e pcs \u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003e 2\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 300px;\"\u003e\n\u003cp\u003e\u003cspan\u003eAdsorb viral nucleic acid\u003c\/span\u003e\u003cspan\u003e and collect the f\u003c\/span\u003e\u003cspan\u003eiltrate\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eStorage conditions\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStore at room temperature (15-25\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e) and transport at room temperature.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eNotes\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1. Prepare your own \u003c\/span\u003e\u003cspan\u003eRN\u003c\/span\u003e\u003cspan\u003ease-free pipette tips, 1.5 ml \u003c\/span\u003e\u003cspan\u003eRN\u003c\/span\u003e\u003cspan\u003ease-free centrifuge tube\u003c\/span\u003e\u003cspan\u003es\u003c\/span\u003e\u003cspan\u003e, centrifuge, etc.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. The virus has a strong ability to infect, \u003c\/span\u003e\u003cspan\u003ea variety of defense measures \u003c\/span\u003e\u003cspan\u003emust be done before the operation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3. Avoid repeated freezing\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003ethawing of samples, otherwise the extracted viral RNA will be degraded and the extracted amount will decrease.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4. All operating procedures, if not specified, are carried out at room temperature (15-25\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5. When using this kit, please wear lab coat, disposable latex gloves, disposable masks and use RNase-free consumables to avoid RNase pollution to the greatest extent.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e6. Please check whether there is crystal precipitation in the Lysis Buffer. If there is crystal precipitation, place it at room temperature or 37\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e until the crystal is dissolved. Mix it before use.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eBefore use:\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAdd 96 ml anhydrous ethanol to \u003c\/span\u003e\u003cspan\u003eWash Buffer\u003c\/span\u003e\u003cspan\u003e, and store at \u003c\/span\u003e\u003cspan\u003eroom temperature\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eProtocol\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1. Add 500\u003c\/span\u003e\u003cspan\u003e μl of Lysis Buffer into\u003c\/span\u003e\u003cspan\u003e a 1.5 ml RNase-free centrifuge tube (self-provided). If there are many samples, the \u003c\/span\u003e\u003cspan\u003eLysis Buffer\u003c\/span\u003e\u003cspan\u003e can be pre-packed.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. Add 200\u003c\/span\u003e\u003cspan\u003e μl\u003c\/span\u003e\u003cspan\u003e of samples, mix thoroughly by vortexing (if the sample is less than 200\u003c\/span\u003e\u003cspan\u003e μl\u003c\/span\u003e\u003cspan\u003e, fill it with normal saline to 200\u003c\/span\u003e\u003cspan\u003e μl\u003c\/span\u003e\u003cspan\u003e).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.\u003c\/span\u003e\u003cspan\u003e T\u003c\/span\u003e\u003cspan\u003eransfer the above mixture to the Spin Column \u003c\/span\u003e\u003cspan\u003e(with\u003c\/span\u003e\u003cspan\u003e Collection Tube\u003c\/span\u003e\u003cspan\u003e)\u003c\/span\u003e\u003cspan\u003e. Centrifuged at 12,000×g for 1 min.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4. Discard the filtrate and put the Spin Column back into the 2 ml Collection Tube. Add 600 \u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003el of Wash Buffer and centrifuge at 12,000×g for 30 sec, then discard the filtrate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNote: make sure the correct amount of anhydrous ethanol has been added to the Wash Buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5. Repeat step 4 once.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e6. Centrifuge for 2 min at 12,000×g to dry the column membrane. Discard the filtrate and collection tube.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e7. Transfer the Spin Column to a new 1.5 ml Collection Tube (provided by the kit), add 50 \u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003el Eluent Buffer to the center of the membrane of the Spin Column, and place it at room temperature for 1 min. Centrifuge at 12,000×g for 1 min.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e8. \u003c\/span\u003e\u003cspan\u003eDiscard the Spin Column\u003c\/span\u003e\u003cspan\u003e, the obtained DNA\/RNA can be directly used for subsequent detection, or be stored at -30 ~ -15\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e for short-term storage or at -70\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e for long-term storage.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003e\u003cbr\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39771534688356,"sku":"V4001-GB","price":214.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/v4001_image_2.jpg?v=1742214049"},{"product_id":"viral-nucleic-acid-dna-rna-miniprep-kit","title":"Viral Nucleic Acid (DNA-RNA) Miniprep Kit","description":"\u003cp\u003e\u003cspan\u003eThe kit adopts the magnetic particle purification technology based on superparamagnetism, which can minimize the risk of cross-contamination and improve the sensitivity and accuracy of detection. The operation time of the instrument is only 50 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eMain components\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe kit consists of the following components: (for a 200 prep kit)\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable cellspacing=\"0\" style=\"width: 488px;\"\u003e\n\u003ctbody\u003e\n\u003ctr class=\"firstRow\"\u003e\n\u003ctd valign=\"top\" style=\"width: 108px;\"\u003e\n\u003cp\u003e\u003cspan\u003eThe name of the reagent\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 100.927px;\"\u003e\n\u003cp\u003e\u003cspan\u003eAmount\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 262.073px;\"\u003e\n\u003cp\u003e\u003cspan\u003eC\u003c\/span\u003e\u003cspan\u003eomponent description\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" style=\"width: 108px;\"\u003e\n\u003cp\u003e\u003cspan\u003eBuffer MVL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 100.927px;\"\u003e\n\u003cp\u003e\u003cspan\u003e45\u003c\/span\u003e\u003cspan\u003e ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 262.073px;\"\u003e\n\u003cp\u003e\u003cspan\u003eProvide environment for \u003c\/span\u003e\u003cspan\u003elysing\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" style=\"width: 108px;\"\u003e\n\u003cp\u003e\u003cspan\u003eBuffer MB\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 100.927px;\"\u003e\n\u003cp\u003e\u003cspan\u003e25\u003c\/span\u003e\u003cspan\u003e ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 262.073px;\"\u003e\n\u003cp\u003e\u003cspan\u003eProvide environment for \u003c\/span\u003e\u003cspan\u003ebinding to the\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003em\u003c\/span\u003e\u003cspan\u003eagnetic \u003c\/span\u003e\u003cspan\u003eb\u003c\/span\u003e\u003cspan\u003eeads\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" style=\"width: 108px;\"\u003e\n\u003cp\u003e\u003cspan\u003eBuffer MW1\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 100.927px;\"\u003e\n\u003cp\u003e\u003cspan\u003e55\u003c\/span\u003e\u003cspan\u003e ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 262.073px;\"\u003e\n\u003cp\u003e\u003cspan\u003eRemove residual proteins and other impurities\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" style=\"width: 108px;\"\u003e\n\u003cp\u003e\u003cspan\u003eMagV Beads\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 100.927px;\"\u003e\n\u003cp\u003e\u003cspan\u003e5.2\u003c\/span\u003e\u003cspan\u003e ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 262.073px;\"\u003e\n\u003cp\u003e\u003cspan\u003eAdsorb viral nucleic acid\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" style=\"width: 108px;\"\u003e\n\u003cp\u003e\u003cspan\u003eDEPC-Water\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 100.927px;\"\u003e\n\u003cp\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003e ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 262.073px;\"\u003e\n\u003cp\u003e\u003cspan\u003eDEPC \u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003e treated\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ewater, RNase - free\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" style=\"width: 108px;\"\u003e\n\u003cp\u003e\u003cspan\u003eCarrier RNA\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 100.927px;\"\u003e\n\u003cp\u003e\u003cspan\u003e450 \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 262.073px;\"\u003e\n\u003cp\u003e\u003cspan\u003eCapture trace nucleic acid\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" style=\"width: 108px;\"\u003e\n\u003cp\u003e\u003cspan\u003eProteinase K\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 100.927px;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 ml \u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003e 2\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 262.073px;\"\u003e\n\u003cp\u003e\u003cspan\u003eLyse\u003c\/span\u003e\u003cspan\u003es\u003c\/span\u003e\u003cspan\u003e proteins\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eb\u003c\/span\u003e\u003cspan\u003eoun\u003c\/span\u003e\u003cspan\u003ed to nucleic acids\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eStorage conditions\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStore \u003c\/span\u003e\u003cspan\u003eCarrier RNA and \u003c\/span\u003e\u003cspan\u003eProteinase K \u003c\/span\u003e\u003cspan\u003eat -20\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e, \u003c\/span\u003e\u003cspan\u003eMagV Beads and DEPC-Water \u003c\/span\u003e\u003cspan\u003eat 2-8\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e, others \u003c\/span\u003e\u003cspan\u003eat room temperature (\u003c\/span\u003e\u003cspan\u003eRT, \u003c\/span\u003e\u003cspan\u003e15-25\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e)\u003c\/span\u003e\u003cspan\u003e,\u003c\/span\u003e\u003cspan\u003e and transport at \u003c\/span\u003e\u003cspan\u003eRT\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eNotes\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1. Prepare your own \u003c\/span\u003e\u003cspan\u003eRN\u003c\/span\u003e\u003cspan\u003ease-free pipette tips, 1.5 ml \u003c\/span\u003e\u003cspan\u003eRN\u003c\/span\u003e\u003cspan\u003ease-free centrifuge tube\u003c\/span\u003e\u003cspan\u003es\u003c\/span\u003e\u003cspan\u003e, centrifuge, etc.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. The virus has a strong ability to infect, \u003c\/span\u003e\u003cspan\u003ea variety of defense measures \u003c\/span\u003e\u003cspan\u003emust be done before the operation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3. Avoid repeated freezing\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003ethawing of samples, otherwise the extracted viral RNA will be degraded and the extracted amount will decrease.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4. All operating procedures, if not specified, are carried out at room temperature (15-25\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5. When using this kit, please wear lab coat, disposable latex gloves, disposable masks and use RNase-free consumables to avoid RNase pollution to the greatest extent.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e6. Please check whether there is crystal precipitation in the \u003c\/span\u003e\u003cspan\u003eBuffer MVL\u003c\/span\u003e\u003cspan\u003e. If there is crystal precipitation, place it at room temperature or 37\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e until the crystal is dissolved. Mix it before use.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eBefore use:\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAdd 55 ml anhydrous ethanol to \u003c\/span\u003e\u003cspan\u003eBuffer MW1, and store at RT\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAdd 58 ml isopropanol to \u003c\/span\u003e\u003cspan\u003eBuffer MB, and store at RT\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ePrepare a 75% ethanol solution.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eProtocol\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003e A: Manual Operation Process\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1. \u003c\/span\u003e\u003cspan\u003eViral lysis. Different lysis procedures should be adopted for different experimental materials, as follows:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eA. L\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eysis of v\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eirus in plasma, serum, cell-free body fluids, and viral stock solution\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e: prepare 10-200 \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el of plasma, serum, cell-free body fluids, or viral stock solution. If the initial amount is less than 200 \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el, use PBS solution or \u003c\/span\u003e\u003cspan\u003eDEPC-Water\u003c\/span\u003e\u003cspan\u003e to make up to 200\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eB. Lysis of virus-infected tissues\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e: \u003c\/span\u003e\u003cspan\u003eprepare \u003c\/span\u003e\u003cspan\u003e10 mg of virus-infected tissues to be ground with liquid nitrogen, and add 200\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el\u003c\/span\u003e\u003cspan\u003e of PBS solution or \u003c\/span\u003e\u003cspan\u003eDEPC-Water\u003c\/span\u003e\u003cspan\u003e to the ground tissues.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eC. Throat swab (with preservation solution)\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e: Vortex vigorously for 1 min, take 200ul for experiment.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eD. Throat swab (dry)\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e: Add 300\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eBuffer MVL\u003c\/span\u003e\u003cspan\u003e and 10\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el\u003c\/span\u003e\u003cspan\u003e Proteinase K, vortex vigorously for 15 sec, mix well. Incubate at 56\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e for 10 min, centrifuge at the highest speed for 1 min, take 200\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el\u003c\/span\u003e\u003cspan\u003e supernatant and add 2\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el\u003c\/span\u003e\u003cspan\u003e Carrier RNA. Continue the experiment from step 4.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. \u003c\/span\u003e\u003cspan\u003eAdd 200\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eBuffer MVL\u003c\/span\u003e\u003cspan\u003e, 10\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el\u003c\/span\u003e\u003cspan\u003e Proteinase K and 2\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el\u003c\/span\u003e\u003cspan\u003e Carrier RNA, vortex vigorously for 15 sec, mix well.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3. \u003c\/span\u003e\u003cspan\u003eIncubate at 56\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e for 10 min\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4. \u003c\/span\u003e\u003cspan\u003eAdd 400\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eBuffer MB and 25\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el \u003c\/span\u003e\u003cspan\u003eMagV Beads\u003c\/span\u003e\u003cspan\u003e to the above mixture\u003c\/span\u003e\u003cspan\u003e, mix well. Let stand for 3-5 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5. \u003c\/span\u003e\u003cspan\u003eTransfer the above mixture to the magnetic separation rack, \u003c\/span\u003e\u003cspan\u003eand let stand \u003c\/span\u003e\u003cspan\u003efor 3-5 minutes to absorb the beads. Carefully discard all solutions.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e6. \u003c\/span\u003e\u003cspan\u003eAdd 500\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el \u003c\/span\u003e\u003cspan\u003eBuffer MW1\u003c\/span\u003e\u003cspan\u003e. \u003c\/span\u003e\u003cspan\u003eVortex mix for 15 seconds\u003c\/span\u003e\u003cspan\u003e. Transfer the reclosion to the new tube.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e7\u003c\/span\u003e\u003cspan\u003e. \u003c\/span\u003e\u003cspan\u003eTransfer the above mixture to the magnetic separation rack, and \u003c\/span\u003e\u003cspan\u003elet stand\u003c\/span\u003e\u003cspan\u003e for 2 minutes to absorb the beads. Carefully discard all solutions.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e8. Add 600 ul 75% ethanol and vortex the mixture for 15 sec\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e9. Transfer the above mixture to the magnetic separation rack, and \u003c\/span\u003e\u003cspan\u003elet stand\u003c\/span\u003e\u003cspan\u003e for 2 minutes to absorb the beads. Carefully discard all solutions.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e10. \u003c\/span\u003e\u003cspan\u003eRepeat steps 7-9 once.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e11. \u003c\/span\u003e\u003cspan\u003eCentrifuge briefly to collect droplets from the wall of the tube.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eTransfer to the \u003c\/span\u003e\u003cspan\u003emagnetic separation rack\u003c\/span\u003e\u003cspan\u003e and carefully discard all solution\u003c\/span\u003e\u003cspan\u003es.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e12. Air drying for 7\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003e10 min.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e13. Add 30~50\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003el \u003c\/span\u003e\u003cspan\u003eDEPC-Water\u003c\/span\u003e\u003cspan\u003e, vortex to disperse magnetic beads.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eLet stand for 3\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003e5 min, during which gently oscillate 1\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003e2 times to accelerate nucleic acid dissolution.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e14. Transfer to the magnetic \u003c\/span\u003e\u003cspan\u003eseparation rack\u003c\/span\u003e\u003cspan\u003e and let stand for 3 min.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eTransfer the nucleic acid solution to a new 1.5 ml centrifuge tube.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"100 preps","offer_id":39771534753892,"sku":"V4002-GB","price":214.65,"currency_code":"CAD","in_stock":true},{"title":"200 preps","offer_id":39771534786660,"sku":"V4002-200-GB","price":403.88,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/noimagedefaultimage-jpeg_87db6362-2456-4b7b-b6e4-61715abb9fff.jpg?v=1662136889"},{"product_id":"zipcut-restriction-enzyme-ecori","title":"ZipCut™ Restriction Enzyme EcoRI","description":"\u003ch3\u003e\u003c\/h3\u003e\n\u003ctable width=\"153\" style=\"font-size: 0.875rem;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"153\"\u003e\n\u003cp\u003eThis ZipCut™ Restriction Enzyme is a rapid cutting endonuclease. It is a member of a series of restriction endonucleases that have undergone genetic engineering recombination and can accurately complete DNA cutting within 5 to 15 minutes, suitable for plasmid DNA, PCR products or genomic DNA, etc. The ZipCut™ Restriction Enzyme has the following characteristics:\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e-enzyme digestion can be completed within 5~15 minutes;\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e-double digests can share a single enzyme digestion buffer, greatly simplify the enzyme digestion reaction setup; good enzyme activity ,\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e-easy to deal with high DNA substrate concentration or difficult template digestion.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e-Recommend using 1 ul per 20 ul reaction, this amount should be adjusted based on the concentration of DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e-provided with 10× Reaction Buffer 1 ml×2 and 10× Reaction Color Buffer 1 ml×2.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e-the Color Buffer enables direct loading of the digested DNA to gel without adding loading buffer.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/ZipCut_Restriction_Enzyme_EcoRI_User_Manual_1.docx?v=1700840788\"\u003e\u003cspan\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"228\" height=\"64\"\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"600 rxns","offer_id":40188830744676,"sku":"GMD042-1","price":68.59,"currency_code":"CAD","in_stock":true},{"title":"3000 rxns","offer_id":40188830777444,"sku":"GMD042-2","price":304.82,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/noimagedefaultimage-jpeg_25ab53ae-9199-45ac-b273-3c889dcb9edd.jpg?v=1653063245"},{"product_id":"zipcut-restriction-enzyme-hindiii","title":"ZipCut™ Restriction Enzyme HindIII","description":"\u003ch3\u003e\u003c\/h3\u003e\n\u003ctable style=\"font-size: 0.875rem;\" width=\"153\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"153\"\u003e\n\u003cp\u003eThis ZipCut™ Restriction Enzyme is a rapid cutting endonuclease. It is a member of a series of restriction endonucleases that have undergone genetic engineering recombination and can accurately complete DNA cutting within 5 to 15 minutes, suitable for plasmid DNA, PCR products or genomic DNA, etc. The ZipCut™ Restriction Enzyme has the following characteristics:\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e-enzyme digestion can be completed within 5~15 minutes;\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e-double digests can share a single enzyme digestion buffer, greatly simplify the enzyme digestion reaction setup; good enzyme activity ,\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e-easy to deal with high DNA substrate concentration or difficult template digestion.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e-Recommend using 1 ul per 20 ul reaction, this amount should be adjusted based on the concentration of DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e-provided with 10× Reaction Buffer 1 ml×2 and 10× Reaction Color Buffer 1 ml×2.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e-the Color Buffer enables direct loading of the digested DNA to gel without adding loading buffer.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/ZipCut_Restriction_Enzyme_HindIII_User_Manual.pdf?v=1709734512\"\u003e\u003cimg height=\"53\" width=\"188\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Gene Bio Systems","offers":[{"title":"500 rxns","offer_id":40188876062820,"sku":"GMD045-1","price":60.96,"currency_code":"CAD","in_stock":true},{"title":"3000 rxns","offer_id":40188876095588,"sku":"GMD045-2","price":266.72,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/noimagedefaultimage-jpeg_ac005b59-23e7-41c2-a7d4-4740dc08b063.jpg?v=1653064065"},{"product_id":"zipcut-restriction-enzyme-bamhi","title":"ZipCut™ Restriction Enzyme BamHI","description":"\u003ch3\u003e\u003c\/h3\u003e\n\u003ctable style=\"font-size: 0.875rem; height: 287px;\" width=\"153\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 113px;\"\u003e\n\u003ctd style=\"height: 113px; width: 556.667px;\"\u003e\n\u003cp\u003eThis ZipCut™ Restriction Enzyme is a rapid cutting endonuclease. It is a member of a series of restriction endonucleases that have undergone genetic engineering recombination and can accurately complete DNA cutting within 5 to 15 minutes, suitable for plasmid DNA, PCR products or genomic DNA, etc. The ZipCut™ Restriction Enzyme has the following characteristics:\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 0px;\"\u003e\n\u003ctd style=\"height: 0px; width: 556.667px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 556.667px;\"\u003e-enzyme digestion can be completed within 5~15 minutes;\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 0px;\"\u003e\n\u003ctd style=\"height: 0px; width: 556.667px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\"\u003e\n\u003ctd style=\"height: 39px; width: 556.667px;\"\u003e-double digests can share a single enzyme digestion buffer, greatly simplify the enzyme digestion reaction setup; good enzyme activity ,\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 0px;\"\u003e\n\u003ctd style=\"height: 0px; width: 556.667px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 556.667px;\"\u003e-easy to deal with high DNA substrate concentration or difficult template digestion.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 0px;\"\u003e\n\u003ctd style=\"height: 0px; width: 556.667px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\"\u003e\n\u003ctd style=\"height: 39px; width: 556.667px;\"\u003e-Recommend using 1 ul per 20 ul reaction, this amount should be adjusted based on the concentration of DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 0px;\"\u003e\n\u003ctd style=\"height: 0px; width: 556.667px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 556.667px;\"\u003e-provided with 10× Reaction Buffer 1 ml×2 and 10× Reaction Color Buffer 1 ml×2.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 0px;\"\u003e\n\u003ctd style=\"height: 0px; width: 556.667px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\"\u003e\n\u003ctd style=\"height: 39px; width: 556.667px;\"\u003e\n\u003cp\u003e-the Color Buffer enables direct loading of the digested DNA to gel without adding loading buffer.\u003c\/p\u003e\n\u003cp\u003e\u003ca aria-describedby=\"a11y-external-message\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/2X_SYBR_Green_qPCR_Mix__High_Rox.pdf?v=1700579015\"\u003e\u003c\/a\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/ZipCut_Restriction_Enzyme_BamHI_User_Manual.pdf?v=1709734294\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\" width=\"188\" height=\"53\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Gene Bio Systems","offers":[{"title":"500 rxns","offer_id":40188883271780,"sku":"GMD032-1","price":76.2,"currency_code":"CAD","in_stock":true},{"title":"3000 rxns","offer_id":40188883304548,"sku":"GMD032-2","price":342.92,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/noimagedefaultimage-jpeg_ed40d32b-1e3f-4ef0-a82a-a159d05f88fb.jpg?v=1653064232"},{"product_id":"lysozyme","title":"Lysozyme","description":"\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eDescription\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eLysozyme is an enzyme used to break down bacterial cell walls to improve protein or nucleic acid extraction efficiency. Lysozyme is a glycoside hydrolase that catalyzes the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan, which is the major component of gram-positive bacterial cell wall. This hydrolysis in turn compromises the integrity of bacterial cell walls causing lysis of the bacteria. Molecular weight 14.4 kDa.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eApplications\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e•Removal of the cell wall of Gram-positive bacteria\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eBiochem\/physiol Functions\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eCatalyzes the hydrolysis of 1,4-β-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycans and between N-acetyl-D-glucosamine residues in chitodextrins\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eDefinition of Activity Unit\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1 unit is that amount of enzyme needed to reduce the absorption value of the enzyme solution by 0.001 per minute. The reaction substrate is a 0.2 mg\/ml micro-wall lysozyme suspension prepared by phosphoric acid buffer solution (0.05 M, PH 6.1).\u003c\/p\u003e\n\u003cp\u003eThis lysozyme product has the specific activity of \u0026gt;20,000 U\/mg. \u003cspan data-mce-fragment=\"1\"\u003eIt functions as an antimicrobial agent by cleaving the peptidoglycan component of bacterial cell walls\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/BE061_Lysozyme_User_Manual.docx?v=1700584546\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cimg height=\"42\" width=\"149\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\" data-mce-selected=\"1\"\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"10g","offer_id":40188895625316,"sku":"LYSM-1","price":190.51,"currency_code":"CAD","in_stock":true},{"title":"50g","offer_id":40188895658084,"sku":"LYSM-2","price":760.78,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/noimagedefaultimage-jpeg_0b27d041-5ef2-4b2d-9200-1c930f5d6f44.jpg?v=1653064942"},{"product_id":"soil-dna-kit","title":"Soil DNA Kit","description":"\u003ch1\u003eIntroduction\u003c\/h1\u003e\n\u003cp\u003eThis product allows rapid and reliable isolation of high-quality genomic DNA from various soil samples. Up to 500 mg soil samples can be processed in 60 minute. The system combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatilityof spin column technology to eliminate PCR inhibiting compounds such as humic acid from soil samples. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel.\u003c\/p\u003e\n\u003ch1\u003eDetails\u003c\/h1\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"1185\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eFeatures\u003c\/td\u003e\n\u003ctd\u003eSpecifications\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Functions\u003c\/td\u003e\n\u003ctd\u003eIsolation DNA from 200-500mg soil sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eApplications\u003c\/td\u003e\n\u003ctd\u003ePCR, southern blot and enzyme digestion, etc.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePurification method\u003c\/td\u003e\n\u003ctd\u003eMini spin column\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePurification technology\u003c\/td\u003e\n\u003ctd\u003eSilica technology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProcess method\u003c\/td\u003e\n\u003ctd\u003eManual (centrifugation or vacuum)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eSoil\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample amount\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e200-500mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eElution volume\u003c\/td\u003e\n\u003ctd\u003e≥30μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTime per run\u003c\/td\u003e\n\u003ctd\u003e≤60 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLiquid carrying volume per column\u003c\/td\u003e\n\u003ctd\u003e800μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBinding yield of column\u003c\/td\u003e\n\u003ctd\u003e100μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch1\u003ePrinciple\u003c\/h1\u003e\n\u003cp\u003eSoil sample is homogenized and then treated in a specially formulated buffer containing detergent to lyse bacteria, yeast, and fungal samples. humic acid, proteins, polysaccharides, and other contaminants are removed using our proprietary Absorber Solution. Binding conditions are then adjusted and the sample is applied to a DNA Mini Column. Two rapid wash steps remove trace contaminants and pure DNA is eluted in low ionic strength buffer. Purified DNA can be directly used in downstream applications without the need for further purification.\u003c\/p\u003e\n\u003ch1\u003eAdvantages\u003c\/h1\u003e\n\u003cul\u003e\n\u003cli\u003eFast - several samples can be extracted in 40 minutes (after digestion)\u003c\/li\u003e\n\u003cli\u003eHigh purity - purified DNA can be directly used in various downstream applications\u003c\/li\u003e\n\u003cli\u003eGood repeatability - silica technology can obtain ideal results every time\u003c\/li\u003e\n\u003cli\u003eHigh recovery - DNA can be recovered at the level of PG\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eKit Contents\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"1185\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eContents\u003c\/td\u003e\n\u003ctd\u003eD314202\u003c\/td\u003e\n\u003ctd\u003eD314203\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNumber of Preps\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e50 Preps\u003c\/td\u003e\n\u003ctd\u003e250 Preps\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHipure DNA Mini Columns II\u003c\/td\u003e\n\u003ctd\u003e50\u003c\/td\u003e\n\u003ctd\u003e250\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2ml Collection Tubes\u003c\/td\u003e\n\u003ctd\u003e50\u003c\/td\u003e\n\u003ctd\u003e250\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2ml Bead Tubes\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e50\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e250\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer SQL\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e60 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e250 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer SDS\u003c\/td\u003e\n\u003ctd\u003e5 ml\u003c\/td\u003e\n\u003ctd\u003e20 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer PS\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e10 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e50 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAbsorber Solution\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e10 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e50 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer GDP\u003c\/td\u003e\n\u003ctd\u003e80 ml\u003c\/td\u003e\n\u003ctd\u003e2 x 200  ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer GW2*\u003c\/td\u003e\n\u003ctd\u003e20 ml\u003c\/td\u003e\n\u003ctd\u003e2 x 50  ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer AE\u003c\/td\u003e\n\u003ctd\u003e15 ml\u003c\/td\u003e\n\u003ctd\u003e30 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage and Stability\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAbsorber Solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"text-decoration: underline;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/D3142_HiPure_Soil_DNA_Kit_2022-GBS.pdf?v=1709224138\"\u003e\u003cimg height=\"52\" width=\"185\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" data-mce-fragment=\"1\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\"\u003e\u003c\/a\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/D101-V21.1MycoBlue_Mycoplasma_Detector-GBS.pdf?v=1709224091\"\u003e\u003c\/a\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"50 Preps","offer_id":40191764070500,"sku":"D314202","price":533.43,"currency_code":"CAD","in_stock":true},{"title":"250 Preps","offer_id":40191764103268,"sku":"D314203","price":2413.15,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/soil_image_2.jpg?v=1742213989"},{"product_id":"room-temp-blood-sample-lysis-kit-for-direct-pcr","title":"Room-Temp Blood Sample Lysis Kit for Direct PCR","description":"\u003cp\u003e \u003c\/p\u003e\n\u003cdiv data-node=\"619b2e6c24b89\" class=\"fl-row fl-row-fixed-width fl-row-bg-none fl-node-619b2e6c24b89\"\u003e\n\u003cdiv class=\"fl-row-content-wrap\"\u003e\n\u003cdiv class=\"fl-row-content fl-row-fixed-width fl-node-content\"\u003e\n\u003cdiv data-node=\"619b2e6c24bc8\" class=\"fl-col-group fl-node-619b2e6c24bc8\"\u003e\n\u003cdiv data-node=\"619b2e6c24c10\" class=\"fl-col fl-node-619b2e6c24c10\"\u003e\n\u003cdiv class=\"fl-col-content fl-node-content\"\u003e\n\u003cdiv data-animation-delay=\"0.0\" data-node=\"619b2e6c24c4e\" class=\"fl-module fl-module-heading fl-node-619b2e6c24c4e pro_tit\"\u003e\n\u003cdiv class=\"fl-module-content fl-node-content\"\u003e\n\u003ch3 class=\"fl-heading\"\u003e\u003cspan class=\"fl-heading-text\"\u003eStorage Conditions\u003c\/span\u003e\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-node=\"619b2e6c24c8a\" class=\"fl-col-group fl-node-619b2e6c24c8a\"\u003e\n\u003cdiv data-node=\"619b2e6c24cc8\" class=\"fl-col fl-node-619b2e6c24cc8\"\u003e\n\u003cdiv class=\"fl-col-content fl-node-content\"\u003e\n\u003cdiv data-animation-delay=\"0.0\" data-node=\"619b2e6c24d06\" class=\"fl-module fl-module-rich-text fl-node-619b2e6c24d06\"\u003e\n\u003cdiv class=\"fl-module-content fl-node-content\"\u003e\n\u003cdiv class=\"fl-rich-text\"\u003e\n\u003cp\u003eStore at 2 ~ 8℃.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-node=\"619b2e6c24d43\" class=\"fl-row fl-row-fixed-width fl-row-bg-none fl-node-619b2e6c24d43\"\u003e\n\u003cdiv class=\"fl-row-content-wrap\"\u003e\n\u003cdiv class=\"fl-row-content fl-row-fixed-width fl-node-content\"\u003e\n\u003cdiv data-node=\"619b2e6c24d81\" class=\"fl-col-group fl-node-619b2e6c24d81\"\u003e\n\u003cdiv data-node=\"619b2e6c24dc7\" class=\"fl-col fl-node-619b2e6c24dc7\"\u003e\n\u003cdiv class=\"fl-col-content fl-node-content\"\u003e\n\u003cdiv data-animation-delay=\"0.0\" data-node=\"619b2e6c24e07\" class=\"fl-module fl-module-heading fl-node-619b2e6c24e07 pro_tit\"\u003e\n\u003cdiv class=\"fl-module-content fl-node-content\"\u003e\n\u003ch3 class=\"fl-heading\"\u003e\u003cspan class=\"fl-heading-text\"\u003eProduct Introduction\u003c\/span\u003e\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-node=\"619b2e6c24e46\" class=\"fl-col-group fl-node-619b2e6c24e46\"\u003e\n\u003cdiv data-node=\"619b2e6c24e85\" class=\"fl-col fl-node-619b2e6c24e85\"\u003e\n\u003cdiv class=\"fl-col-content fl-node-content\"\u003e\n\u003cdiv data-animation-delay=\"0.0\" data-node=\"619b2e6c24ec3\" class=\"fl-module fl-module-rich-text fl-node-619b2e6c24ec3\"\u003e\n\u003cdiv class=\"fl-module-content fl-node-content\"\u003e\n\u003cdiv class=\"fl-rich-text\"\u003e\n\u003cp\u003eRoomTemp TM Sample Lysis Kit is a simple and fast blood lysis kit at room temperature. This kit contains two components, Lysis Buffer and Stabilizing Buffer, among which Lysis Buffer contains special components that quickly destroy cell membrane proteins and membrane structure, which can be fully released The genomic DNA in the cell, Stabilizing Buffer is added with protective proteins and stabilizing factors, which can eliminate the inhibitory effect of the inhibitor in the lysed sample on the downstream qPCR and PCR reactions, so that the lysed DNA solution can be stored stably for a long time. Applicable blood sample types include Fresh blood, frozen blood and conventional anticoagulant blood (EDTA, citrate, sodium heparin, etc.). Genomic DNA can be released from whole blood samples after 3 minutes of lysis at room temperature. The lysed DNA solution can be directly used as a template ，Apply to Taqman ® probe method for SNP detection, qPCR probe method quantification, PCR amplification, etc. It can achieve the same effect as traditional genome extraction methods without complicated extraction operations. In addition to blood, this kit can also Compatible with pyrolysis FTA blood cards, oral swabs, plant tissues and other samples.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-node=\"619b2e6c24f02\" class=\"fl-row fl-row-fixed-width fl-row-bg-none fl-node-619b2e6c24f02\"\u003e\n\u003cdiv class=\"fl-row-content-wrap\"\u003e\n\u003cdiv class=\"fl-row-content fl-row-fixed-width fl-node-content\"\u003e\n\u003cdiv data-node=\"619b2e6c24f41\" class=\"fl-col-group fl-node-619b2e6c24f41\"\u003e\n\u003cdiv data-node=\"619b2e6c24f80\" class=\"fl-col fl-node-619b2e6c24f80\"\u003e\n\u003cdiv class=\"fl-col-content fl-node-content\"\u003e\n\u003cdiv data-animation-delay=\"0.0\" data-node=\"619b2e6c24fbf\" class=\"fl-module fl-module-heading fl-node-619b2e6c24fbf pro_tit\"\u003e\n\u003cdiv class=\"fl-module-content fl-node-content\"\u003e\n\u003ch3 class=\"fl-heading\"\u003e\u003cspan class=\"fl-heading-text\"\u003eProduct Advantages\u003c\/span\u003e\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-node=\"619b2e6c24fff\" class=\"fl-col-group fl-node-619b2e6c24fff\"\u003e\n\u003cdiv data-node=\"619b2e6c2503e\" class=\"fl-col fl-node-619b2e6c2503e\"\u003e\n\u003cdiv class=\"fl-col-content fl-node-content\"\u003e\n\u003cdiv data-animation-delay=\"0.0\" data-node=\"619b2e6c2507d\" class=\"fl-module fl-module-rich-text fl-node-619b2e6c2507d\"\u003e\n\u003cdiv class=\"fl-module-content fl-node-content\"\u003e\n\u003cdiv class=\"fl-rich-text\"\u003e\n\u003cp\u003eSimple operation: 3 minutes at room temperature, no need for complex template extraction.\u003cbr\u003eHigh lysis efficiency: The lysis reagent has the same effect as traditional kits for extracting genome.\u003cbr\u003eWide range of applications: Compatible with Taqman ® probe method for SNP detection, qPCR probe method quantification, PCR amplification and other sample compatibility: lysis of fresh blood, frozen blood, conventional anticoagulant blood, tissue homogenate, oral swabs, Various samples such as FTA card.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Gene Bio Systems","offers":[{"title":"250 preps","offer_id":40259149725796,"sku":"P073-01","price":240.05,"currency_code":"CAD","in_stock":true},{"title":"1000 preps","offer_id":40259149758564,"sku":"P073-02","price":760.78,"currency_code":"CAD","in_stock":true},{"title":"5000 preps","offer_id":40259149791332,"sku":"P073-03","price":2792.91,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/noimagedefaultimage-jpeg_7c1dfe96-8c6f-407a-b928-a0270c6726a9.jpg?v=1655063074"},{"product_id":"dnase-i-rnase-free","title":"DNase I (RNase-free)","description":"\u003cdiv class=\"acontentc\"\u003e\n\u003cp\u003eDeoxyribonuclease I (DNase I) is an endonuclease that degrades double and single-strand DNA and chromatin. It functions by hydrolyzing phosphodiester linkages, producing mono and oligonucleotides with a 5'-phosphate and a 3'-hydroxyl group. Ribonuclease has been reduced to non-detectable levels. Its activity depends on Mg\u003csup\u003e2+\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eor Mn\u003csup\u003e2+\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eion. DNase I with Mg\u003csup\u003e2+\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecuts randomly double strand DNA at any site, DNase I with Mn\u003csup\u003e2+\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecuts double strand DNA at the same site to form sticky-end with 1-2 nucleotide or form blunt-end.\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan class=\"fontstyle0\"\u003eConcentration:\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"fontstyle0\"\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003cspan class=\"fontstyle0\"\u003e3 units\/μl\u003c\/span\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003cbr\u003eAt -20˚C\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"acontentc\"\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cspan class=\"author\"\u003e\u003cstrong\u003eProduct Contents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"acontentc\"\u003e\n\u003ctable border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr class=\"firstRow\"\u003e\n\u003ctd\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003eGD201-01\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDNase I (3 units\/μl)\u003c\/td\u003e\n\u003ctd\u003e1500 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10×DNase I Reaction Buffer\u003c\/td\u003e\n\u003ctd\u003e2×1 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e200 mM EDTA\u003c\/td\u003e\n\u003ctd\u003e1 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca rel=\"noopener\" title=\"DNase I (RNase-free) Manual - GBS Nov 2024\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/DNase_I_RNAse-free_GBS_Manual_-_Nov_2024.pdf?v=1732281237\" target=\"_blank\"\u003e\u003cspan\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"149\" height=\"42\"\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cdiv class=\"clear\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cspan class=\"author\"\u003e\u003cstrong\u003eCitations\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"acontentc\"\u003e\n\u003cp\u003eZhang G, Wang Z, Du Z, et al. mTOR Regulates Phase Separation of PGL Granules to ModulateTheir Autophagic Degradation[J] Cell, 2018, 174(6):1492-1506.\u003c\/p\u003e\n\u003cp\u003eLiu Z Y , Yao X Q , Zhang Q , et al. Modulation of the Acetone\/Butanol Ratio during Fermentation of Corn StoverDerived Hydrolysate by Clostridium beijerinckii Strain NCIMB 8052[J]. Applied \u0026amp; Environmental Microbiology, 2017, 83(7):AEM.03386-16.\u003c\/p\u003e\n\u003cp\u003eWang S , Wang C , Zhang X X , et al. Transcriptome de novo assembly and analysis of differentially expressed genes related to cytoplasmic male sterility in cabbage[J]. Plant Physiology \u0026amp; Biochemistry Ppb, 2016, 105:224-232.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Gene Bio Systems","offers":[{"title":"500uL","offer_id":40594634965092,"sku":"GD201-01","price":175.27,"currency_code":"CAD","in_stock":true},{"title":"1mL","offer_id":40594634997860,"sku":"GD201-02","price":297.96,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/noimagedefaultimage-jpeg_bc88088b-e5b9-4218-bb26-2178b6b0e181.jpg?v=1662573600"},{"product_id":"hipure-universal-rna-kit","title":"HiPure Universal RNA Kit","description":"\u003cdiv\u003e\n\u003ch3\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eFor isolation (extraction) of total RNA from a large number of sample types, including cultured cells, tissues, plants and microbial samples. \u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003cul\u003e\n\u003cli\u003ePhenol\/guanidine-based lysis and silica membrane purification of total RNA\u003c\/li\u003e\n\u003cli\u003eMagZol Reagent is a monophasic solution of phenol and guanidine thiocyanate, facilitates lysis of samples and inhibits RNases\u003c\/li\u003e\n\u003cli\u003eHigh lysis efficiency of reagent and subsequent removal of contaminants by organic phase extraction enables up to 200ug total purified RNA from less than 100mg animal plant, fungal samples, bacterial cells and other samples\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cbr\u003e\u003cbr\u003e\u003ca style=\"font-size: 0.875rem;\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/R4130_HiPure_Universal_RNA_Kit_-_GBS.pdf?v=1700842764\"\u003e\u003cimg height=\"60\" width=\"213\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\"\u003e\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"50 preps","offer_id":41365767815268,"sku":"R413002","price":237.51,"currency_code":"CAD","in_stock":true},{"title":"250 preps","offer_id":41365767848036,"sku":"R413003","price":1065.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/Screenshot2022-12-30095750.jpg?v=1672412858"},{"product_id":"hipure-blood-dna-kit","title":"HiPure Blood DNA Mini Kit","description":"\u003ch1\u003eIntroduction\u003c\/h1\u003e\n\u003cp\u003e\u003cspan\u003eThis product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch1\u003eDetails\u003c\/h1\u003e\n\u003cp\u003e\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"1185\"\u003e\n\u003ctbody\u003e\n\u003ctr class=\"firstRow\"\u003e\n\u003ctd\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eSpecifications\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eMain Functions\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eIsolation total DNA from 200ul Whole Blood\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eApplications\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003ePCR, southern bolt and virus detection, etc\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003ePurification method\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eMini spin column\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003ePurification technology\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eSilica technology\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eProcess method\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eManual (centrifugation or vacuum)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eSample type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eBlood, serum, plasma, milk, saliva, and other liquid samples and cultured cells\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eSample amount\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10-250ul\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eElution volume\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e≥20μl\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eTime per run\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e≤30 minutes\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eLiquid carrying volume per column\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e800µl\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eBinding yield of column\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e100µg\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch1\u003ePrinciple\u003cbr\u003e\n\u003c\/h1\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cspan\u003eThis product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).\u003c\/span\u003e\u003c\/p\u003e\n\u003ch1\u003eAdvantages\u003c\/h1\u003e\n\u003cul class=\"list-paddingleft-2\"\u003e\n\u003cli\u003e\u003cspan\u003eHigh quality DNA - meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eFast - without separation of leukocytes, organic extraction or ethanol precipitation\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eSimple - all nucleic acids can be obtained by direct digestion\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eWide applicability- handle a variety of liquid samples\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/D3111_GBS-Manual-HiPure-Blood-DNA-Mini-Kit_Jan-2025.pdf?v=1738328864\" target=\"_blank\" rel=\"noopener\"\u003e\u003cspan\u003e\u003cimg height=\"53\" width=\"188\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\"\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eKit Contents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable width=\"1185\"\u003e\n\u003ctbody\u003e\n\u003ctr class=\"firstRow\"\u003e\n\u003ctd\u003e\n\u003cspan\u003eC\u003c\/span\u003e\u003cspan\u003eon\u003c\/span\u003e\u003cspan\u003et\u003c\/span\u003e\u003cspan\u003eent\u003c\/span\u003e\u003cspan\u003es\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eD311102\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\"\u003e\u003cspan\u003eD311103\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cspan\u003ePurifi\u003c\/span\u003e\u003cspan\u003ecation Times\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e50\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\"\u003e\u003cspan\u003e250\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eHiPure DNA Mini Columns I\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e50\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\"\u003e\u003cspan\u003e2 x 125\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cspan\u003e2ml Collection Tubes\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\"\u003e\u003cspan\u003e5 x 100\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eBuffer AL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e15 ml\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\"\u003e\u003cspan\u003e60 ml\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eBuffer DW1\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e30 ml\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\"\u003e\u003cspan\u003e150 ml\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eBuffer GW2\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e12 ml\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\"\u003e\u003cspan\u003e50 ml\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eProteinase K\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e24 mg\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\"\u003e\u003cspan\u003e120 mg\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eProtease Dissolve Buffer\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e1.8 ml\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\"\u003e\u003cspan\u003e10 ml\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cspan\u003eBuffer AE\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e15 ml\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\"\u003e\u003cspan\u003e60 ml\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003e \u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003eStorage and Stability\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cspan\u003eProteinase K should be stored at 2–8°Cupon arrival. However, short-term storage (up to 12 weeks) at room temperature(15–25°C) does not affect their performance. The remaining kit components canbe stored at room temperature (15–25°C) and are stable for at least 18 monthsunder these conditions.\u003c\/span\u003e\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"50 prep","offer_id":41449707012196,"sku":"D3111","price":204.42,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/Screenshot_28.png?v=1674658628"},{"product_id":"hipure-universal-dna-kit","title":"HiPure Universal DNA Kit","description":"\u003ch1\u003eIntroduction\u003c\/h1\u003e\n\u003cp\u003eThe HiPure Universal DNA Kit—a reliable and efficient solution for the quick extraction of total DNA from various clinical samples, such as tissue, cells, blood, saliva, swabs, blood spots, and semen. The extracted DNA is ready for immediate use in PCR, qPCR, Southern Blot analysis, and viral DNA testing.\u003c\/p\u003e\n\u003ch1 style=\"font-weight: 400;\"\u003e\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003c\/h1\u003e\n\u003ctable style=\"font-weight: 400;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eFeatures\u003c\/td\u003e\n\u003ctd\u003eSpecifications\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Functions\u003c\/td\u003e\n\u003ctd\u003eIsolation total DNA from tissue \/ blood \/ body fluid \/ swab \/dry blood spots\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eApplications\u003c\/td\u003e\n\u003ctd\u003ePCR, qPCR, southern bolt and virus detection, etc\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePurification method\u003c\/td\u003e\n\u003ctd\u003eMini spin column\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePurification technology\u003c\/td\u003e\n\u003ctd\u003eSilica technology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProcess method\u003c\/td\u003e\n\u003ctd\u003eManual (centrifugation or vacuum)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eTissue, cell, blood, saliva, swab, blood spot,semen and other clinical samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample amount\u003c\/td\u003e\n\u003ctd\u003eSolid tissue: 1-10mg; Anticoagulant blood: 200μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eElution volume\u003c\/td\u003e\n\u003ctd\u003e≥20μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTime per run\u003c\/td\u003e\n\u003ctd\u003e30 - 60 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLiquid carrying volume per column\u003c\/td\u003e\n\u003ctd\u003e800µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBinding yield of column\u003c\/td\u003e\n\u003ctd\u003e100µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch1\u003ePrinciple\u003c\/h1\u003e\n\u003cp\u003eThis kit utilizes \u003cstrong\u003esilica column-based purification technology.\u003c\/strong\u003e The process starts with the lysis and proteolytic digestion of the sample, releasing DNA into the lysate. The lysate is then transferred to an adsorption column where nucleic acids bind to the membrane. Proteins and other impurities are washed away, and the purified nucleic acid is eluted with a low-salt buffer (10 mM Tris, pH 9.0, 0.5 mM EDTA).\u003c\/p\u003e\n\u003ch1\u003eAdvantages\u003c\/h1\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh quality DNA \u003c\/strong\u003e- meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFast\u003c\/strong\u003e - without separation of leukocytes, organic extraction or ethanol precipitation\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSimple\u003c\/strong\u003e - all nucleic acids can be obtained by direct digestion\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide applicability\u003c\/strong\u003e- handle a \u003cstrong\u003evariety of liquid samples\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch1\u003eKit Contents\u003c\/h1\u003e\n\u003ctable width=\"1185\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eContents\u003c\/td\u003e\n\u003ctd\u003eD301802\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003eD301803\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePurification Times\u003c\/td\u003e\n\u003ctd\u003e50\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e250\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHiPure DNA Mini Columns I\u003c\/td\u003e\n\u003ctd\u003e50\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e250\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2ml Collection Tubes\u003c\/td\u003e\n\u003ctd\u003e100\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer ATL\u003c\/td\u003e\n\u003ctd\u003e30 ml\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e150 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer AL\u003c\/td\u003e\n\u003ctd\u003e30 ml\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e150 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer GW1\u003c\/td\u003e\n\u003ctd\u003e22 ml\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e88 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer GW2\u003c\/td\u003e\n\u003ctd\u003e12 ml\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e50 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProteinase K\u003c\/td\u003e\n\u003ctd\u003e24 mg\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e120 mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProtease Dissolve Buffer\u003c\/td\u003e\n\u003ctd\u003e1.8 ml\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e10 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer AE\u003c\/td\u003e\n\u003ctd\u003e15 ml\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e60 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003eStorage and Stability\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eProteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/D3018_HiPure_Univesal_DNA_Kit_2022-GBS.pdf?v=1700840973\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"245\" height=\"69\"\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"50 preps","offer_id":41449829171300,"sku":"D301802","price":306.03,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/20250328_153747.jpg?v=1743595442"},{"product_id":"hipure-ffpe-dna-kit","title":"HiPure FFPE DNA Kit","description":"\u003ch1 data-mce-fragment=\"1\"\u003eIntroduction\u003c\/h1\u003e\n\u003cp data-mce-fragment=\"1\"\u003eHiPure FFPE Nucleic acid Kit supplies a simple and rapid DNA extraction for formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 20 minutes (not including digestion time). DNA can be directly used for downstream applications such as PCR, southern blot and viral DNA detection, etc.\u003c\/p\u003e\n\u003ch1 data-mce-fragment=\"1\"\u003eDetails\u003c\/h1\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"1185\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eFeatures\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eSpecifications\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eMain Functions\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eIsolation total DNA from FFPE tissue samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003ePCR, southern blot and viral DNA detection, etc.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003ePurification method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eMini spin column\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003ePurification technology\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eSilica technology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eProcess method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eManual (centrifugation or vacuum)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eSample type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eFormalin-fixed, paraffin-embedded (FFPE) tissue and sections samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eSample amount\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u0026lt;20mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eElution volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u0026lt;20µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eTime per run\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u0026gt;≤20 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eLiquid carrying volume per column\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e4ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBinding yield of column\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e100μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003ch1 data-mce-fragment=\"1\"\u003ePrinciple\u003c\/h1\u003e\n\u003cp data-mce-fragment=\"1\"\u003eHipure FFPE Nuclear acid kit adopts silica gel column purification. The sample is deparaffinated by xylene and digested by lysate and protease. After de crosslinked at 90 ℃, DNA\/RNA is released into the lysate. Adding ethanol to adjust the binding conditions, the sample is transferred to the column where DNA\/RNA is adsorbed on the membrane and protein is removed without adsorption. Protein and other impurities are washed by buffer GW1, and the salt is removed by buffer GW2. Finally, the DNA \/ RNA is eluted by low salt buffer.\u003c\/p\u003e\n\u003ch1 data-mce-fragment=\"1\"\u003eAdvantages\u003c\/h1\u003e\n\u003cul data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003eSafety - deparaffinating without contacting with xylene or other toxic solution\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003eFast - without overnight incubation and digestion, several samples can be extracted within 2hours\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003eHigh efficiency -remove the formaldehyde modification of DNA, greatly enhancing the sensitivity of PCR\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003ch1 data-mce-fragment=\"1\"\u003eKit Contents\u003c\/h1\u003e\n\u003ctable width=\"1185\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eContents\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eD312602\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003eD312603\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003ePurification Times\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50 Preps\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e250\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003ePreps\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eHiPure DNA Mini Columns I\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e250\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2ml Collection Tubes\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e250\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBuffer DPS\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e30 ml\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e150 ml\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBuffer ATL\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e15 ml\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e60 ml\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBuffer AL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e15 ml\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e60 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBuffer GW1*\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e22 ml\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e88 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBuffer GW2*\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e12 ml\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e50 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eProteinase K\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e24 mg\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e120 mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eProtease Dissolve Buffer\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1.8 ml\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e10 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBuffer AE\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e10 ml\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e30 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage and Stability\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eProteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"50 preps","offer_id":41449896018020,"sku":"D312602","price":240.71,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/Screenshot_30.png?v=1674660395"},{"product_id":"hipure-plant-dna-mini-kit","title":"HiPure Plant DNA Mini Kit","description":"\u003ch1 data-mce-fragment=\"1\"\u003e\n\u003cspan style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eIntroduction\u003c\/span\u003e\u003cbr\u003e\n\u003c\/h1\u003e\n\u003cp data-mce-fragment=\"1\"\u003eHiPure Plant DNA Mini Kit supplies a simple and rapid extraction of genomic DNA from different plant samples. The kit is based on silica gel column and CTAB lysis purification technology. The whole extraction process is only 30-50 minutes. Purified DNA can be used directly for PCR, SSR, AFLP, RAPD and Southern Blot, etc.\u003c\/p\u003e\n\u003ch1 data-mce-fragment=\"1\"\u003eDetails\u003c\/h1\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable data-mce-fragment=\"1\" width=\"1185\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eFeatures\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eSpecifications\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eMain Functions\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eIsolation total DNA from 150 mg plant and fungal tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003ePCR, SSR, AFLP, RAPD and southern blot, etc.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003ePurification method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eMini spin column\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003ePurification technology\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eSilica technology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eProcess method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eManual (centrifugation or vacuum)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eSample type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eFresh \/ frozen plant samples, dried plant \/ seed samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eSample amount\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eFresh \/ frozen plant samples: 50-150 mg\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eDried plant \/ seed samples:\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e15-40\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003emg\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eElution volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e≥40μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eTime per run\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e30-50\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003eminutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eLiquid carrying volume per column\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e800μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBinding yield of column\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e100μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003ch1 data-mce-fragment=\"1\"\u003ePrinciple\u003c\/h1\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThis product is based on silica column purification. The sample is lysed with CATB Buffer. DNA is released into the lysate. Cell debris, precipitated proteins and polysaccharides are removed by chloroform extraction. After adjust the binding condition, transfer to an adsorption column. DNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).\u003c\/p\u003e\n\u003ch1 data-mce-fragment=\"1\"\u003eAdvantages\u003c\/h1\u003e\n\u003cul data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003eBroad spectrum - suitable for extracting DNA from various plant samples\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003eFast - several samples can be extracted in 30 minutes by silica technology\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003eGood repeatability - silica gel column purification technology can obtain ideal results every time\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eKit Contents\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable data-mce-fragment=\"1\" width=\"1185\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eContents\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eD318702\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eD318703\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003ePurification Times\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50 Preps\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e250 Preps\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBuffer PAL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e60 ml\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e200\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003eml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBuffer GWP\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e60 ml\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e200 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBuffer DW1\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e30 ml\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e150 ml\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBuffer GW2*\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20 ml\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003eml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBuffer AE\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20 ml\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e60 ml\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eHiPure DNA Mini Columns II\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e250\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2 ml Collection Tubes\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e250\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage and Stability\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThis product can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/D3187_HiPure_HP_Plant_DNA_Kit_202212_2_-GBS.pdf?v=1700593984\" data-mce-href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/D3187_HiPure_HP_Plant_DNA_Kit_202212_2_-GBS.pdf?v=1700593984\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cimg data-mce-fragment=\"1\" height=\"42\" width=\"149\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" data-mce-selected=\"1\"\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"50 preps","offer_id":41449948086372,"sku":"D318702","price":204.42,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/Screenshot_31.png?v=1674660897"},{"product_id":"hipure-sf-plant-dna-kit","title":"HiPure SF Plant DNA Kit","description":"\u003ch1 data-mce-fragment=\"1\"\u003e\n\u003cspan style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eIntroduction\u003c\/span\u003e\u003cbr\u003e\n\u003c\/h1\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThis product provides a fast and easy way to purify DNA from plant and fungal tissue. Up to 100 mg of tissue can be processed. Easy-to-use Plant procedures provide pure total DNA (genomic, mitochondrial, and chloroplast) for reliable PCR and southern blotting in less than 1 hour. Purification requires no phenol or chloroform extraction or alcohol precipitation and involves minimal handling.\u003c\/p\u003e\n\u003ch1 data-mce-fragment=\"1\"\u003eDetails\u003c\/h1\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"1185\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eFeatures\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eSpecifications\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eMain Functions\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eIsolation total DNA from100 mg simple plant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003ePCR, SSR, AFLP, RAPD and southern blot, etc.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003ePurification method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eMini spin column\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003ePurification technology\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eSilica technology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eProcess method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eManual (centrifugation or vacuum)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eSample type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eEconomic plant samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eSample amount\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eFresh \/ frozen plant samples:\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e≤100m\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003egDried plant \/ seed samples:\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e≤20mg\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eElution volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e≥30μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eTime per run\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e30-50\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003eminutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eLiquid carrying volume per column\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e800μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBinding yield of column\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e100μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003ch1 data-mce-fragment=\"1\"\u003ePrinciple\u003c\/h1\u003e\n\u003cp data-mce-fragment=\"1\"\u003ePlantmaterial is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated.Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged. DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.\u003c\/p\u003e\n\u003ch1 data-mce-fragment=\"1\"\u003eAdvantages\u003c\/h1\u003e\n\u003cul data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003eSafe - require no phenol or chloroform extraction\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003eFast - several samples can be extracted in 30 minutes by silica technology\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003eHigh purity - high quality DNA, completely remove inhibitors\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003eHigh yield - silica technology can achieve the highest yield\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eKit Contents\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"1185\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eContents\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eD316402\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eD316403\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003ePurification Times\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50 Preps\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e250 Preps\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eRNase A\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e10 mg\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50 mg\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eProtease Dissolve Buffer\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1.8 ml\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5 ml\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBuffer SPL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e30 ml\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e150 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBuffer PS\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e10 ml\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBuffer GW1\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e22 ml\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e110 ml\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBuffer GW2*\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e12 ml\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2 x 50\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003eml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBuffer AE\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e15 ml\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e60 ml\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eHiPure gDNA Mini Columns\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2 x 125\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2 ml Collection Tubes\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2 x 125\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage and Stability\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eRNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/D3164_HiPure_SF_Plant_DNA_Kit-GBS.pdf?v=1715553820\" data-mce-href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/D3164_HiPure_SF_Plant_DNA_Kit-GBS.pdf?v=1715553820\"\u003e\u003cstrong\u003e\u003cimg height=\"69\" width=\"245\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\" data-mce-selected=\"1\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\"\u003e\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"50 preps","offer_id":41449976922212,"sku":"D316402","price":204.42,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/20250328_152253.jpg?v=1743596976"},{"product_id":"solpure-tissue-dna-kit","title":"SolPure Tissue DNA Kit","description":"\u003ch1 data-mce-fragment=\"1\"\u003eIntroduction\u003c\/h1\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThis product is designed for purification of high-molecular-weight genomic or mitochondrial DNA from a variety of tissue and cultured cells. The convenient, scalable purification procedure removes contaminants and enzyme inhibitors such as proteins and divalent cation, and purified DNA is ready for immediate use in sensitive downstream applications or for archiving.\u003c\/p\u003e\n\u003ch1 data-mce-fragment=\"1\"\u003eDetails\u003c\/h1\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eFeatures\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eSpecifications\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eMain Functions\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eIsolation total DNA from tissue using economic salt out method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003ePCR, enzyme digestion, Southern hybridization,etc.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003ePurification technology\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eSalting out method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eProcess method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eManual (centrifugation or vacuum)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eSample type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eAnimal tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eSample amount\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eUnlimited\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eElution volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e≥100μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eTime per run\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eVariation with sample amount\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003ePrinciples\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eCells are lysed with an anionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, such as proteins, are removed by salt precipitation. Finally, the genomic DNA is recovered by precipitation with alcohol and dissolved in Buffer TE. Purified DNA typically has an A260\/A280 ratio between 1.7 and 1.9, and is up to 200 kb in size. The DNA can be safely stored at 2-8°C, -20°C, or -80°C.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003ch1 data-mce-fragment=\"1\"\u003eAdvantages\u003c\/h1\u003e\n\u003cul data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003eSafe - without phenol chloroform extraction\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003eEnvironment friendly - the reagents used are safe, non-toxic and without pollution\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003eHigh molecular weight - the molecular weight of genomic DNA is about 50-150kb\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003eHigh purity - the purified DNA has A260\/280=1.8-1.9, A260\/230=2.0-2.5\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003eUnlimited sample size - solution type operation, sample volume can be adjusted at will\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003eCost\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003eperformance - the most economical nucleic acid extraction technology\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003ch1 data-mce-fragment=\"1\"\u003eKit Contents\u003c\/h1\u003e\n\u003ctable data-mce-fragment=\"1\" style=\"width: 551px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 215.523px;\"\u003eContents\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 103.477px;\"\u003eD331201\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\" style=\"width: 99px;\"\u003eD331202\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 110px;\"\u003eD331203\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 215.523px;\"\u003e\n\u003cp\u003eScale - grams of sample\u003c\/p\u003e\n\u003cp\u003e( number of preps)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 103.477px;\"\u003e\n\u003cp\u003e1 g \u003c\/p\u003e\n\u003cp\u003e\u003cspan data-mce-fragment=\"1\"\u003e(100preps)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\" style=\"width: 99px;\"\u003e\n\u003cp\u003e5 g \u003c\/p\u003e\n\u003cp\u003e\u003cspan data-mce-fragment=\"1\"\u003e(500preps)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 110px;\"\u003e\n\u003cp\u003e50 g\u003c\/p\u003e\n\u003cp\u003e\u003cspan data-mce-fragment=\"1\"\u003e(5000preps)\u003c\/span\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 215.523px;\"\u003eProteinase K\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 103.477px;\"\u003e330 µl\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\" style=\"width: 99px;\"\u003e1.8 ml\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 110px;\"\u003e18 ml\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 215.523px;\"\u003eBuffer WTL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 103.477px;\"\u003e33 ml\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\" style=\"width: 99px;\"\u003e160\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003eml\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 110px;\"\u003e2 x 800 ml\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 215.523px;\"\u003eBuffer PPS\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 103.477px;\"\u003e12\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003eml\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\" style=\"width: 99px;\"\u003e55\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003eml\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 110px;\"\u003e500\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003eml\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 215.523px;\"\u003eRNase Solution\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 103.477px;\"\u003e330 µl\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\" style=\"width: 99px;\"\u003e1.8 ml\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 110px;\"\u003e18 ml\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 215.523px;\"\u003eBuffer\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003eTE\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 103.477px;\"\u003e12 ml\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\" style=\"width: 99px;\"\u003e60 ml\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 110px;\"\u003e200\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003eml\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch1 data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage and Stability\u003c\/strong\u003e\u003c\/h1\u003e\n\u003cp data-mce-fragment=\"1\"\u003eRNase Solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/D3312_SolPure_Tissue_DNA_Kit-GBS.pdf?v=1705431947\"\u003e\u003cstrong\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cimg data-mce-fragment=\"1\" height=\"43\" width=\"153\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" data-mce-selected=\"1\"\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"1 g (100preps)","offer_id":41449993896036,"sku":"D331201","price":200.79,"currency_code":"CAD","in_stock":true},{"title":"5 g (500preps)","offer_id":42673635229796,"sku":"D331202","price":809.22,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/Screenshot_33.png?v=1674661475"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/collections\/DNA_and_RNA_isolation_collection_image.jpg?v=1662146072","url":"https:\/\/www.genebiosystems.com\/en-us\/collections\/rna-isolation-and-dna-isolation.oembed?page=4","provider":"GeneBio ","version":"1.0","type":"link"}