{"title":"Reverse Transcription","description":"\u003cp\u003e\u003cspan style=\"color: #fbe329;\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"color: #000000;\"\u003eReverse transcription is the process of converting RNA back to DNA. Since PCR and many other amplification technologies work on DNA substrates, RNA must be reverse transcribed to cDNA. In reverse transcription, RNA is first bound by a primer, which is then extended in the presence of nucleotide triphosphates (dNTPs), in a reaction that is catalyzed by reverse transcriptase. Products include RT-qPCR kits, with one step or Q select options. \u003c\/span\u003e\u003c\/p\u003e","products":[{"product_id":"rt-pcr-kit-cdna-synthesis-kit","title":"RT-PCR Kit ( cDNA synthesis kit)","description":"\u003cdiv class=\"body\"\u003e\n\u003cdiv class=\"w-container w-main\"\u003e\n\u003cdiv class=\"row\"\u003e\n\u003cdiv class=\"wrap-content-in w-system w-productcom\"\u003e\n\u003cdiv class=\"w-system-in clearfix\"\u003e\n\u003cdiv class=\"product-detail-wrap\"\u003e\n\u003cdiv class=\"w-com-content\"\u003e\n\u003cdiv class=\"product-maincon\"\u003e\n\u003cdiv class=\"product-maincon-in\"\u003e\n\u003cdiv class=\"product-descons\"\u003e\n\u003cdiv class=\"descon_item\" id=\"Tabitem234864\"\u003e\n\u003cp\u003e\u003cspan\u003eRT-PCR Kit is optimized to synthesize first-strand cDNA from purified poly(A)+ or total RNA. The RT-pcr kit for RT-PCR delivers increased cDNA yields, high sensitivity, and full-length transcripts in a convenient format. You get all of the components you need for successful first-strand cDNA synthesis, saving your time and ensuring your success with every experiment. The kit Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme is used to synthesize cDNA at a temperature range of 42-55\u003cspan\u003e°\u003c\/span\u003eC, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eApplications \u003cbr\u003eFirst strand cDNA synthesis for RT-PCR . \u003cbr\u003eConstruction of cDNA libraries. \u003cbr\u003eOne-step RT-PCR \u003cbr\u003ePrimer extension\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eComponents of the kit \u003c\/span\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eCompenent\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eµl\u003cstrong\u003e (20 rxns)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eµl\u003cstrong\u003e (100 rxns)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eM-MLV (200 U\/µl)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e20\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e100\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eRNAsine (40 U\/ µl)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e12\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e60\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eOligo (dT)15 (50 µM)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e20\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e100\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eRandom hexame (50 µM)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e20\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e100\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e5x First-strand buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e80\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e400\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eRnase-free ddH2O\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1 ml x 5\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003edNTP (10 mM each)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e50\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e200\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStorage\u003cbr\u003eAll components of the kit should be stored at -20\u003cspan\u003e°\u003c\/span\u003eC. Keep control RNA at -70\u003cspan\u003e°\u003c\/span\u003eC for longer storage.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eImportant notes \u003cbr\u003eAvoiding ribonuclease contamination \u003cbr\u003eRNA purity and integrity is essential for synthesis of full-length cDNA. RNA can be degraded RNase A, which is a highly stable contaminant found in any laboratory environment. All components of the kit have been rigorously tested to ensure that they are RNase free. To prevent contamination both the laboratory environment and all prepared solutions must be free of RNases. General recommendations to avoid RNase contamination:\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eDEPC-treat all tubes and pipette tips to be used in cDNA synthesis or use certified nuclease-free labware.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eWear gloves when handling RNA and all reagents, as skin is a common source of RNases. Change gloves frequently.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eUse RNase-free reagents, including high quality water (e.g., DEPC-treated Water).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eUse an RNase inhibitor to protect RNA from the activity of RNases.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eKeep all kit components tightly sealed when not in use. Keep all tubes tightly closed during the reverse transcription reaction.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003cspan\u003eTemplate RNA \u003cbr\u003eTotal cellular RNA isolated by standard methods is suitable for use with the kit. Purified RNA must be free of salts, metal ions, ethanol and phenol to avoid inhibiting the cDNA synthesis reaction. Trace contaminants can be removed by ethanol precipitation of the RNA followed by two washes of the pellet with cold 75% ethanol. For RT-PCR applications, template RNA must be free of DNA contamination. Prior to cDNA synthesis, RNA can be treated with DNase I to remove trace amounts of DNA (DNase I is not included in this kit). Always perform a control (RT-minus) reaction which includes all components for RT-PCR except for the reverse transcriptase enzyme.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eRNase H Digestion \u003cbr\u003eThe sensitivity of the PCR step can be increased (especially for long templates) by removing the RNA template from the cDNA:RNA hybrid molecule by digestion with RNase H after first-strand synthesis. Presence of RNase H during first-strand synthesis will degrade the template mRNA, resulting in decreased full-length cDNA synthesis and decreased yields of first-strand cDNA.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ePrimers \u003cbr\u003eSynthesis of first strand cDNA can be primed with either oligo(dT)15 primer, random primers or gene-specific primers.\u003cbr\u003eOligo(dT)15 primes cDNA synthesis from the poly(A) tail present at the 3\u003cspan\u003e’\u003c\/span\u003e-end of eukaryotic mRNA. Random Primers initiate cDNA synthesis from the total RNA population (rRNA and mRNA). Therefore, using random primers for first strand synthesis results in a greater complexity of the generated cDNA compared with the oligo(dT)15 primer. As a consequence, the sensitivity and specificity of subsequent PCR reactions may be reduced. However, there are several applications where it is beneficial to use random primers, such as cDNA synthesis using mRNAs without a poly(A) tail, or cDNA synthesis using poly(A)-enriched RNA samples.\u003cbr\u003eGene-specific primers are used to synthesize specifi c cDNA from a pool of total RNA or mRNA and must be obtained by the user.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFirst Strand cDNA synthesis procedure \u003cbr\u003eThe first strand cDNA reaction can be performed as an individual reaction or as a series of parallel reactions with different RNA templates. Therefore, the reaction mixture can be prepared by combining reagents individually or a master mix containing all of the components except template RNA can be prepared. Depending on the structure of the RNA template, separate steps for RNA denaturation and primer annealing may improve RT-PCR results.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eProtocol \u003cbr\u003eI. First strand cDNA synthesis\u003cbr\u003e1. Add the following reagents into a sterile, nuclease-free tube on ice in the indicated order:\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eTemplate RNA\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003ePoly (A) mRNA or specific RNA\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1-5 µg\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003ePrimer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eOligo (dT)15 Primer or Randome hexamer primer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1 µg\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eDEPC-treated water\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"2\"\u003e\n\u003cp\u003e\u003cspan\u003e To 12.4 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan\u003e\u003cbr\u003e2. Mix gently, centrifugebriefly and incubate at 70\u003cspan\u003e°\u003c\/span\u003eC for 5 min. Chill on ice, spin down and place the vial back on ice.\u003cbr\u003e3. Prepare the following cDNA Synthesis Mix, add the following components in the indicated order:\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e5x First-strand buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e4 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003edNTPs(10 mM each)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eRnasine\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e0.6 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eM-MLV\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4.Mix gently and centrifuge\u003cbr\u003e5.For oligo(dT)15, incubate for 60 min at 42\u003cspan\u003e°\u003c\/span\u003eC. For random hexamer primed synthesis, incubate for 60 min at 37\u003cspan\u003e°\u003c\/span\u003eC .\u003cbr\u003e6.Terminate the reaction by heating at 70\u003cspan\u003e°\u003c\/span\u003eC for 5 min. \u003cbr\u003eThe reverse transcription reaction product can be used immediately in second strand cDNA synthesis reactions or stored at -20\u003cspan\u003e°\u003c\/span\u003eC for less than a week. For longer storage, -70\u003cspan\u003e°\u003c\/span\u003eC is recommended.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eII. PCR Amplification of First Strand cDNA\u003cbr\u003eThe product of the first strand cDNA synthesis can be used directly in PCR or qPCR. The volume of first strand cDNA synthesis reaction mixture should not comprise more than 1\/10 of the total PCR reaction volume. Normally, 2 \u003cspan\u003eμ\u003c\/span\u003el of the first strand cDNA synthesis reaction mixture is used as template for subsequent PCR in 50 \u003cspan\u003eμ\u003c\/span\u003el total volume.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/R1011-R1012_RT-PCR_Kit_V1.0_1_-GBS.pdf?v=1700836884\"\u003e\u003cspan\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"160\" height=\"45\"\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Gene Bio Systems","offers":[{"title":"20 Preps","offer_id":39766544220260,"sku":"R1011","price":103.47,"currency_code":"CAD","in_stock":true},{"title":"100 Preps","offer_id":39766544253028,"sku":"R1012","price":354.29,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_ee950fd4-ff2b-49fa-b323-cba524f27fc4.jpg?v=1704394367"},{"product_id":"m-mlv-reverse-transcriptase","title":"M-MLV Reverse Transcriptase","description":"\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003eThe MMLV \u003c\/strong\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eReverse Transcriptase(short name MMLV) i is iolated from E.coli carrying rat leukemia virus pol gene consists of a single peptide, molecule weight 71kd.It possesses RNA and DNA depended polymerase activity and weak RNase H activity.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eConcentration: \u003c\/strong\u003e 200U\/μl\u003cbr\u003e\u003cstrong\u003eComponent : \u003c\/strong\u003e M-MLV （200U\/μl） 5×Buffer （with DTT）\u003cbr\u003e\u003cstrong\u003ePackage：\u003c\/strong\u003e Bulk\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFeatures:\u003c\/strong\u003e\u003cbr\u003eWeak RNaseH activity; High cDNA yield\u003cbr\u003e\u003cstrong\u003eStoreage: \u003c\/strong\u003e-20℃\u003cbr\u003eApplication：Synthesis of the first chain cDNA, cDNA Library construction, one-step RT-PCR, primer extention, 3′ and 5′RACE\u003cbr\u003e\u003cstrong\u003eSource:\u003c\/strong\u003e Recombination of E.coli containing Moloney murine leukemia virus reverse transcriptase gene from clone of Moloney murine .\u003cbr\u003e\u003cstrong\u003eUnit definition:\u003c\/strong\u003eOne unit is defined as the required enzyme incorporate 1 nm dNTP into a polynucleotide fraction in 10 min at 37℃, taking polyA﹒poly(dT)12-18 as template-primer.\u003cbr\u003e\u003cstrong\u003eProcedure:\u003c\/strong\u003e\u003cbr\u003e1. add the next reaction mixture to ice bath tube :\u003cbr\u003e1) template RNA\u003cbr\u003etotal RNA 0.1-5μg\u003cbr\u003eor total poly(A)+mRNA 0.1-0.5μg\u003cbr\u003eor unique RNA 0.01pg-0.5μg\u003cbr\u003e2) primer\u003cbr\u003eOligo(dT)18 (0.5μg\/μl) 1μl\u003cbr\u003eOr stochastic primer（0.2μg\/μl） 1μl\u003cbr\u003eOr sequence especially primer 20pmol\u003cbr\u003e3) RNase-free ddh2o : constant volume to 11μl\u003cbr\u003e2. Gently mix and water bath for 5 min in 70℃ and chill on ice.\u003cbr\u003e3. Put the tube into ice and add the next composition :\u003cbr\u003e5×Reaction Buffer 4μl\u003cbr\u003eRNase Inhibitor (40U\/μl) 0.5μl\u003cbr\u003edNTP Mix(10mmol\/L) 2μl\u003cbr\u003eadd water to 19ul ,gently mix and then water bath for 5 min in 37℃ ; or for 5 min in 25℃ for random primer \u003cbr\u003e4. Spin down for a few seconds. Add 1μl M-MLV RT（200U\/μl）\u003cbr\u003e5. Incubate at 42℃ for 60min(if use a random primer,first incubate for 10min in 25℃\u003cbr\u003e6. Inactivate at 70℃ for 10min.\u003cbr\u003e\u003cstrong\u003ePCR Reaction\u003c\/strong\u003e\u003cbr\u003e1. Transfer 10% volume of first reaction solution (2 μl ) to a proper PCR tube .\u003cbr\u003enote: the first reaction solution can be directly used as PCR template without purification ,the dosage is about 1-5μl. if excessively used ,the salt and Random primers in first reaction solution will restrain the activity of DNA polymerase .if purification needed ,it can follow the next :after reaction end of cDNA synthesis (step 6) , add RNase A in reaction system , 10 min in 37℃ ,use DP1501 recover cDNA .\u003cbr\u003e2. add next solution by order .\u003cbr\u003e5μl 10X PCR Buffer\u003cbr\u003e1μl 10mM dNTP mix\u003cbr\u003e1μl 10μM Primer #1 (customer supplied)\u003cbr\u003e1μl 10μM Primer #2 (p customer supplied)\u003cbr\u003exμl H20 (total reaction volume:49μl)\u003cbr\u003e1μl Taq DNA polymerase\u003cbr\u003e3. Mix thoroughly and add 50μl mineral oil to the surface of liquid.\u003cbr\u003e4. Amplified reaction : according to annealing temperature or gene copy number or technical parameter of Taq DNA polymerase , setting amplified condition , specify reference to specification of DNA polymerase ,the usually cycle number is 30-35\u003cbr\u003e5 Detect the product in agarose containing EB or another fluorescent dye.\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"5000U","offer_id":39766547136612,"sku":"R1041","price":87.8,"currency_code":"CAD","in_stock":true},{"title":"25000U","offer_id":39903278661732,"sku":"R1042","price":384.07,"currency_code":"CAD","in_stock":true},{"title":"50000U","offer_id":42761391505508,"sku":"R1043","price":702.31,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/SealingFilmGuide.png?v=1765475529"},{"product_id":"redscripttm-m-mlv-reverse-transcriptase-h","title":"REDscript(TM) M-MLV Reverse Transcriptase (H-)","description":"\u003cp\u003e\u003cstrong\u003eREDScript(TM)\u003c\/strong\u003e \u003cstrong\u003eM-MLV Reverse Transcriptase\u003c\/strong\u003e, encoded by Moloney Murine Leukemia Virus (\u003cstrong\u003eMMLV RT\u003c\/strong\u003e) is an RNA-dependent DNA polymerase that synthesizes the complementary DNA (cDNA) first strand from a single-stranded RNA template to which a primer has been hybridized. M-MLV RT will also extend primers hybridized to single-stranded DNA. Second strand cDNA synthesis can be achieved from some mRNA templates without an additional DNA polymerase. The difference between this to the general M-MLV RT is that the capacity to endure the heat is enhanced. It can remain the 100% activity at 50℃, it can also keep more than 75% activity at even at 55℃.\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"Thermal Stable M-MLV Reverse RH-\" src=\"http:\/\/www.mebep.com\/uploads\/allimg\/110608\/1-11060Q64Z1224.jpg\"\u003e\u003cimg alt=\"Thermal Stable M-MLV\" src=\"http:\/\/www.mebep.com\/uploads\/allimg\/110608\/1-11060QA021I1.jpg\"\u003e\u003c\/p\u003e\n\u003cp\u003eUpper panel: Template: Mouse total RNA, Reverse transcription at 50℃\u003c\/p\u003e\n\u003cp align=\"left\"\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eLane 1-2: \u003cstrong\u003eREDscript(TM) M-MLV\u003c\/strong\u003e\u003cstrong\u003e 200U \u003c\/strong\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"left\"\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eLane 3-4 :invitrogen SuperScript® III \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"left\"\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eLane 5-6:Takara m-mlv \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"left\"\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eLane M: Marker \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"left\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eLower panel: Template: Mouse total RNA, Reverse transcription at 55℃\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eLane 1-2:Takara m-mlv\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eLane 3-4: \u003cstrong\u003eREDscript(TM) M-MLV\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e Lane 5-6:200U invitrogen SuperScript® III\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"left\"\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eLane M: Marker \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003eSource: Recombination of E.coli containing Moloney murine leukemia virus reverse transcriptase gene from clone of Moloney murine.\u003cbr\u003eConcentration: 200U\/μl\u003cbr\u003eComponent: M-MLV （200U\/μl）\u003cbr\u003e 5×Buffer （with DTT）\u003cbr\u003e Package： \u003cstrong\u003eBulk\u003c\/strong\u003e\u003cbr\u003eFeatures: Weak RNaseH activity High cDNA yield\u003cbr\u003eStoreage: -20℃\u003c\/p\u003e\n\u003cp\u003eUnit Definition: One unit of \u003cstrong\u003eREDscript(TM) M-MLV\u003c\/strong\u003e RT catalyzes\u003cbr\u003ethe incorporation of 1 nmol of dTTP into acidinsoluble material in 10 minutes at 37℃ using oligo(dT)12-18-primed poly(A)n as a template.\u003c\/p\u003e\n\u003cp\u003eApplications: The first-strand cDNA synthesis; RT-PCR.\u003c\/p\u003e\n\u003cp\u003eStorage Buffer: 20 mM Tris-HCl (pH7.5), 200 mM NaCl，0.25 mM EDTA, 0.01% NP-40(v\/v)，2.5 mM DTT,50% glycerol (v\/v).\u003cbr\u003e5X Reaction Buffer:\u003cbr\u003e[5×RT Buffer] 250mM Tris-HCl (pH 8.3), 15mM MgCl2，375 mM KCl，50mM DTT.\u003cbr\u003eRecommended Reaction Conditions:\u003cbr\u003eThe first-strand cDNA synthesis\u003cbr\u003e1) Add the following reagents to a RNase free PCR tube at room temperature add the MMLV RT last.\u003cbr\u003eOligo dT12-18 (1μg\/μl) or random primer (50-250ng) 1μl\u003cbr\u003eTotal RNA (10ng-5μg) or mRNA(1-500ng) xμl\u003cbr\u003edNTP (10mM each) 1μl\u003cbr\u003eDEPC ddH2O (14-x)μl\u003cbr\u003e2) Gently mix and incubate 10 Min at 70℃ then chill on ice for 2-10min.\u003cbr\u003e3) Centrifuge for a few seconds then Put the tube into ice and add the next composition :\u003cbr\u003e5×RT Buffer 4μl\u003cbr\u003eRNasin (40U\/μl) 1μl\u003cbr\u003e5) Gently mix and incubate at 50℃ for 2 Min（Oligo dT12-18 or sequence especially primer）\u003cbr\u003eor at 25℃ for 10 min for the random primer.\u003cbr\u003e6) Centrifuge for a few seconds. Add 1μl \u003cstrong\u003eREDscript(TM) \u003c\/strong\u003eM-MLV RT（200U\/μl）Incubate at 50℃ for 50min.\u003cbr\u003e7) Inactivate at 70℃ for 10min then get the cDNA.\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"5000 U","offer_id":39766556606564,"sku":"PCR-05","price":127.01,"currency_code":"CAD","in_stock":true},{"title":"25,000 U","offer_id":39766556639332,"sku":"PCR-05","price":533.43,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/326.jpg?v=1643677692"},{"product_id":"ribonuclease-inhibitor","title":"Ribonuclease Inhibitor","description":"\u003cp\u003eRibonuclease Inhibitor, Human Placenta is a recombinant human placental protein with specifically inhibits broad-spectrum RNase such as RNase A, RNase B and RNase C. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H. It does not inhbit Taq DNA polymerase and T7 RNA Polymerase. The 50kDa protein exerts its inhibitory effect by noncovalently binding to RNases at 1:1 ratio. The Ki value for binding of Rnase Inhibitor, Human Placenta to RNase is approximately 10(-14)M.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFeature:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eImproved resistance to oxidation, compared to human\/porcine Rnase inhibitor\u003c\/p\u003e\n\u003cp\u003eIdeal for reactions where low DTT concentrations are required (e.g. Real-time PCR)\u003c\/p\u003e\n\u003cp\u003eSource An E.coli strain that carries Ribonuclease Inhibitor gene from human placenta.\u003c\/p\u003e\n\u003cp\u003eVolume activity 40U\/ul\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003eSource An E.coli strain that carries Ribonuclease Inhibitor gene from human placenta. Recombinant\u003c\/p\u003e\n\u003cp\u003eVolume activity 40U\/ul\u003c\/p\u003e\n\u003cp\u003eUnit Definition of Rnase in One unit is defined as the amount of Recombinant \u003cem\u003eRibonuclease Inhibitor\u003c\/em\u003e required inhibit the activity of 5ng of ribonuclease A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2’3’-cyclic monophosphate by ribonuclease A.\u003c\/p\u003e\n\u003cp\u003eStorage Temperature -20°C\u003c\/p\u003e\n\u003cp\u003eStorage Buffer 20mM HEPES-KOH(PH7.6), 50mM KCl, 8mM DTT, 50%(v\/v) glycerol.\u003c\/p\u003e\n\u003cp\u003eQuality Control: Absence of Endonuclease 1μg of Lambda DNA is incubated with 200 units of Ribonuclease Inhibitor for 16 hour at 37°C. Following incubation, Lambda DNA is visualized as intact on an ethidium bromide-stained agarose gel to verify the absence of visible Endonuclease. RNase Inhibitor, Murine is a 50 kDa recombinant protein of murine origin. The inhibitor specifically inhibits RNases A, B and C. It inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from\u003cem\u003e Aspergillus\u003c\/em\u003e. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor is used with \u003ca href=\"http:\/\/www.mebep.com\/a\/Products\/Enzyme\/Taq_DNA_Polymerase\/2011\/0505\/Taq_DNA_Polymerase.html\"\u003e\u003cem\u003eTaq\u003c\/em\u003e DNA Polymerase\u003c\/a\u003e, AMV or\u003ca href=\"http:\/\/www.mebep.com\/a\/Products\/Enzyme\/M-MLV_Reverse_Transcriptase\/2011\/0505\/Thermal_stable_M-MLV_Reverse.html\"\u003e M-MuLV Reverse Transcriptases\u003c\/a\u003e, or Phage RNA Polymerases (SP6, T7, or T3).\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"2000 U","offer_id":39766556770404,"sku":"R201-01","price":54.43,"currency_code":"CAD","in_stock":true},{"title":"25,000 U","offer_id":39766556803172,"sku":"R201-02","price":604.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/327.jpg?v=1643677697"},{"product_id":"gb-script-iii-reverse-transcriptase","title":"GB-Script™  III Reverse Transcriptase","description":"\u003cp\u003e\u003cstrong\u003e Components                                                   R302-01 10,000 U\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e5x \u003cspan\u003eGB-Script™ \u003c\/span\u003e IIIBuffer                                           500 μl\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eGB-Script™ \u003c\/span\u003e III Reverse Transcriptase (200 U\/ μl)   50 μl\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/R302-HiScript__III_Reverse_Transcriptase-GBS.pdf?v=1700837753\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"213\" height=\"60\"\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"10000U (50µL)","offer_id":39887074918500,"sku":"R302-01","price":419.13,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_62e08d08-28e4-4b3c-a57a-d04b613b5dce.jpg?v=1694782749"},{"product_id":"gb-script-iii-1-st-strand-cdna-synthesis-kit-gdna-wiper","title":"GB-Script™  III 1 st Strand cDNA Synthesis Kit (+ gDNA wiper)","description":"\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eThis product delivers high efficiency of first strand cDNA synthesis. The 5× gDNA wiper Mix in the kit can quickly remove genomic DNA contamination at 42℃ for 2 min, which makes the results more reliable and simplifies qPCR primer design process without the need to design primers across introns. The kit contains two separated primers: the reverse transcription primers Oligo (dT) 20 VN and Random hexamers, allowing users to choose primers flexibly for subsequent experiments as needed. The kit can synthesize full-length cDNA (up to 20 kb) for downstream experiments such as cloning, and can also synthesize a highly uniform cDNA for qPCR quantification.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/R312-V21.1HiScript_III_1st_Strand_cDNA-GBS.pdf?v=1700838009\" data-mce-href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/R312-V21.1HiScript_III_1st_Strand_cDNA-GBS.pdf?v=1700838009\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"228\" height=\"64\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 273px;\" width=\"720\" data-mce-style=\"height: 273px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eR312-01  50\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003erxn \u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003e(20\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eμl\/rxn)\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eR312-02 \u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003e100\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003erxn \u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003e(20\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eμl\/rxn)\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eRNasefreeddH\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003eO\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e5× gDNAwiperMix\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e100 μl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e200 μl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e10x \u003cspan\u003eRT\u003c\/span\u003eMix\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e100 μl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e200 μl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eGB-Script™ \u003c\/span\u003e III EnzymeMix\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e100 μl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e200 μl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eOligo (dT)\u003c\/span\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eVN\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e50 μl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e100 μl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eRandomHexamers\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e50 μl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e100 μl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Gene Bio Systems","offers":[{"title":"50 reactions","offer_id":39771525283940,"sku":"R312-01","price":440.16,"currency_code":"CAD","in_stock":true},{"title":"100 reactions","offer_id":39771525316708,"sku":"R312-02","price":827.68,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_1f7fc20e-7292-4a41-85e3-e7287408449a.jpg?v=1706709612"},{"product_id":"hiscript-r-ii-one-step-qrt-pcr-probe-kit","title":"HiScript® II One Step qRT-PCR Probe Kit","description":"\u003cp\u003e\u003cimg alt=\"\" src=\"http:\/\/www.vazymebiotech.com\/imageRepository\/990234f9-2584-4498-8fa9-9ffaea05b8cc.png\"\u003e\u003cstrong\u003eSize:\u003c\/strong\u003e 100 Rxn (50 ul\/Rxn)\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eIt is critically challenging for detection of low-copy templates and weak-positive samples in clinical diagnosis. HiScript\u003csup\u003e®\u003c\/sup\u003e II One Step qRT-PCR Probe Kit contains the optimal proportion of HiScript\u003csup\u003e®\u003c\/sup\u003e II Reverse Transcriptase and Champagne Taq\u003csup\u003eTM\u003c\/sup\u003e DNA Polymerase and optimized buffer system. No matter for total RNA, in-vitro-transcribed RNA or virus RNA, HiScript\u003csup\u003e®\u003c\/sup\u003e II One Step qRT-PCR Probe Kit shows extremely high sensitivity and stable amplification performance. Multi amplification can also achieve without much optimization.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39771529609316,"sku":"Q222","price":518.92,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_6d0d3247-1516-4793-ad75-7b0cac73f5c4.jpg?v=1705589195"},{"product_id":"hiscript-ii-u-one-step-qrt-pcr-probe-kit","title":"HiScript® II U One Step qRT-PCR Probe Kit","description":"\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cimg alt=\"\" src=\"http:\/\/www.vazymebiotech.com\/imageRepository\/231fe888-713d-4b26-8d95-8864c40f60f5.png\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eHiScript II U+ One Step qRT-PCR Probe Kit  is specially designed for qPCRs that directly use RNA (i.e. virus RNA) as templates. Using\u003c\/span\u003e\u003c\/span\u003e \u003cspan\u003e\u003cspan\u003egene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube, significantly reducing pipetting procedures and the risk of\u003c\/span\u003e\u003c\/span\u003e \u003cspan\u003e\u003cspan\u003econtamination. This kit contains a dUTP\/ UDG system that can prevent the contamination of PCR product. The HiScript II Reverse Trancriptase and hot-start\u003c\/span\u003e\u003c\/span\u003e \u003cspan\u003e\u003cspan\u003eChampagne Taq DNA Polymerase in this kit enable high-sensitive total RNA detection (as little as 0.1 pg or \u0026lt; 10 copies). The HiScript II U+ One Step qRT-PCR\u003c\/span\u003e\u003c\/span\u003e \u003cspan\u003e\u003cspan\u003eProbe Kit is provided in master mix. The 2× One Step U+ Mix contains an optimized buffer and dNTP\/dUTP mix, and is suitable for high-sensitive detection\u003c\/span\u003e\u003c\/span\u003e \u003cspan\u003e\u003cspan\u003esystems based on fluorescence labelled probes (i.e. TaqMan®).\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cimg alt=\"\" src=\"http:\/\/www.vazymebiotech.com\/imageRepository\/5b10290c-416e-4665-9583-26cc1da5b588.png\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ctable border=\"1\" cellpadding=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cstrong\u003e\u003cspan\u003e\u003cspan\u003eCATALOG\u003c\/span\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cstrong\u003e\u003cspan\u003e\u003cspan\u003eSIZE\u003c\/span\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eQ222-CN-00\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e100 tests\/kit\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eQ222-CN\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e5,000 tests\/kit\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cimg alt=\"\" src=\"http:\/\/www.vazymebiotech.com\/imageRepository\/74d90b1a-3a12-4eed-bc96-58a76dfaf7b6.png\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eAll components should be stored at -20\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003cspan\u003e℃\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39771529642084,"sku":"Q223","price":518.92,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_19fdf326-0fdb-4b84-aecd-0e91ed66c885.jpg?v=1705589123"},{"product_id":"hiscript-iii-rt-supermix-for-qpcr-gdna-wiper","title":"HiScript® III RT SuperMix for qPCR (+gDNA wiper)","description":"\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eThe 4 × gDNA wiper Mix in the kit can completely remove the remaining genomic DNA in the RNA template, ensure that the subsequent quantitative results are more reliable, and simplify the design of qPCR primers without the need to design primers across introns; 5 × HiScript III qRT SuperMix contains All components required for reverse transcription reaction can be reacted quickly by adding template RNA and water, and at the same time, the gDNA wiper function is terminated to ensure the integrity of cDNA. The reverse transcription product is compatible with dye method and probe method qPCR, which can perform high-performance gene expression analysis. \u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eEasy and quick operation: 5 × HiScript III qRT SuperMix contains all the components required for reverse transcription reaction, only need to add template RNA, the reverse transcription reaction can be completed within 20 minutes;\u003cbr\u003eExtensive template compatibility: Compatible with RNA templates of different species and poor integrity;\u003cbr\u003eSuper impurity tolerance: It has super tolerance to common reverse transcription impurities such as ethanol, isopropanol, water balance phenol, guanidine isothiocyanate, humic acid, etc.;\u003cbr\u003eExcellent reverse transcription efficiency: Compared with common commercial reverse transcription products, HiScript ® III RT SuperMix for qPCR (+gDNA wiper) reverses the smallest cDNA quantitative C T value and has excellent reverse transcription efficiency.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/R323_GBS-Manual_Jan-2025.pdf?v=1738600412\" rel=\"noopener\" target=\"_blank\"\u003e\u003cimg data-mce-fragment=\"1\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"228\" height=\"64\"\u003e\u003c\/a\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/R323-V22.1-GBS.pdf?v=1700840382\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eStore at -30~-15°C\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"100 rxns","offer_id":40170552492132,"sku":"R323-01","price":712.45,"currency_code":"CAD","in_stock":true},{"title":"200 rxns","offer_id":42760224702564,"sku":"R323-02","price":1282.42,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_c65bb98d-e643-4f52-a7b0-d365895a3571.jpg?v=1706708940"},{"product_id":"hiscript-ii-one-step-rt-pcr-kit","title":"HiScript® II One Step RT-PCR Kit","description":"\u003cp\u003e\u003cstrong\u003eSize: 125 rxn (20 uL\/reaction)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eValidation Results:\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eA. Taking total RNA of Hela cells as template, the GAPDH fragment of 555 bp was amplified using HiScript II One Step RT-PCR Kit (#P611). The detection sensitivity can reach 0.1 pg.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eB. Taking PPRSV RNA as template, a fragment of 660 bp was amplified using HiScript II One Step RT-PCR Kit (#P611). The detection sensitivity can reach 0.2 TCLD50.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eC. Taking 1 μg of total RNA from Hela cells as template, fragments with different lengths were amplified using HiScript II One Step RT-PCR Kit (#P611). The UTRN fragments as long as 12.1 kb were successfully amplified.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan lang=\"en-us\"\u003eThe HiScript II One Step RT-PCR Kit is specially designed for RNA detection (such as RNA virus). With the HiScript II One Step RT-PCR Kit and gene-specific primers (GSP), both reverse transcription and PCR amplification are performed in the same tube, with no additional pipetting procedures, which improves detection through-put and minimizes potential contamination. This kit contains HiScript II Reverse Transcriptase, Champagne Taq plus hot-start DNA Polymerase, and an optimized buffer, which enables high-sensitive total RNA detection and long-fragment amplification (as long as 10 kb).\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eP611-01 50 rxn (50 ul\/rxn)\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eRNase free ddH\u003csub\u003e2\u003c\/sub\u003eO\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e1 ml\u003c\/span\u003e×\u003cspan\u003e2\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e ×\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eOne Step Mix\u003csup\u003ea\u003c\/sup\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e1 ml\u003c\/span\u003e×\u003cspan\u003e2\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eOne Step Enzyme Mix\u003csup\u003eb\u003c\/sup\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e125 μl\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e ×\u003c\/span\u003e\u003cspan\u003e Loading buffer\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e1.25 ml\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003ea. Contains dNTPs.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eb. Contains RNase inhibitor, HiScript II Reverse Transcriptase, and Champagne Taq plus Polymerase.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eStorage: \u003cspan\u003eAll components should be stored at -20\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/P611_HiScript_II_One_Step_RT-PCR-GBS.pdf?v=1700835445\"\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"149\" height=\"42\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39771529969764,"sku":"P611","price":325.38,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_f6729fcc-282f-4157-b415-8aa8879ef785.jpg?v=1706711014"},{"product_id":"hiscript-ii-one-step-rt-pcr-supermix-dye-plus","title":"HiScript® II One Step RT-PCR SuperMix (Dye Plus)","description":"\u003cp\u003e\u003cstrong\u003eSize: 50 rxn (50 ul\/rxn) \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe HiScript II One Step RT-PCR SuperMix (Dye Plus) is specially designed for RNA detection (such as RNA virus). With the HiScript II One Step RT-PCR SuperMix and gene-specific primers (GSP), both reverse transcription and PCR amplification are performed in the same tube, with no additional pipetting procedures, which improves detection through-put and minimizes potential contamination. This SuperMix contains HiScript II Reverse Transcriptase, RNase inhibitor, and Champagne Taq plus hot-start DNA Polymerase, which enables high-sensitive total RNA detection (as little as 1 pg) and long-fragment amplification (as long as 10 kb). The 2\u003c\/span\u003e\u003cspan\u003e× \u003c\/span\u003e\u003cspan\u003eOne Step Mix (Dye Plus) contains an opimized buffer, dNTPs, and Loading Dye.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"http:\/\/www.vazymebiotech.com\/imageRepository\/5b10290c-416e-4665-9583-26cc1da5b588.png\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"0\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003ecomponents\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eP612-01 50 rxn (50 ul\/rxn)\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eRNase free ddH\u003csub\u003e2\u003c\/sub\u003eO\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e1 ml×2\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp align=\"left\"\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003eOne Step Mix\u003csup\u003ea\u003c\/sup\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e625 μl×2\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eOne Step Enzyme Mix\u003csup\u003eb\u003c\/sup\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e125 μl\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp align=\"left\"\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003ea. Contains dNTPs and Loading Dye.\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eb. Contains RNase inhibitor, HiScript II Reverse Transcriptase, and Champagne Taq plus Polymerase.\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStore at -20 C. \u003c\/span\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39771530002532,"sku":"P612","price":325.38,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_1f8631f3-ebf4-4168-bdc4-70fac6e5aa2f.jpg?v=1706710983"},{"product_id":"one-step-probe-rt-qpcr-kit","title":"One-step Probe RT-qPCR Kit","description":"\u003cp\u003e\u003cstrong\u003eSize:\u003c\/strong\u003e 100 rxns\u003c\/p\u003e\n\u003cp\u003eHeat-labile UDG can rapidly degrade the pollutants containing U at room temperature. \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eWhen reverse transcription is performed at 55°C, heat-labile UDG will be inactivated rapidly without affecting the efficiency and sensitivity of RT-qPCR.\u003c\/span\u003e\u003cspan\u003e Integrating the superior performance of Reverse Transcriptase and hot-start\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eTaq DNA Polymerase, with the optimized buffer system, the detection sensitivity of \u003c\/span\u003e\u003cspan\u003eOne-step\u003c\/span\u003e\u003cspan\u003e Probe\u003c\/span\u003e\u003cspan\u003e RT-qPCR Kit\u003c\/span\u003e\u003cspan\u003e can reach 0.1 pg total RNA or \u0026lt;10 copies of RNA template.\u003c\/span\u003e\u003cspan\u003e The kit is available as a convenient Master Mix. 2 × One-step U\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e+\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e Mix contains an optimized buffer system and dNTP\/dUTP Mix, which is suitable for high-specificity detection systems with fluorescent labeled probes such as TaqMan\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e®\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e. This product is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-Rad, etc.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e\u003cspan\u003eApplications\u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003cspan\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Probe gene expression analysis\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Probe Low-copy gene detection\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Probe microarray validation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Probe gene knockdown validation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003cspan\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• For One-step RT-qPCR operation  \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• This kit is suitable for fluorescence quantification by probe method\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• This kit is compatible with many real-time systems\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Hot-start technology brings high specificity and reproducible amplification\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• dUTP\/UDG system, effectively prevent PCR product contamination\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e• Multiplex detection\u003c\/p\u003e\n\u003cp\u003e• Supports high-performance PCR detection\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39771531116644,"sku":"v5001","price":361.67,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_eade14e5-44d6-4c53-a643-4cab79e07288.jpg?v=1705589082"},{"product_id":"murine-rnase-inhibitor","title":"Murine RNase Inhibitor","description":"\u003ch2 data-start=\"311\" data-end=\"368\"\u003eReliable RNase Protection for Sensitive RNA Workflows\u003c\/h2\u003e\n\u003cp data-start=\"370\" data-end=\"665\" class=\"\"\u003eMurine RNase Inhibitor is a high-performance, recombinant enzyme derived from mouse and expressed in \u003cem data-start=\"471\" data-end=\"480\"\u003eE. coli\u003c\/em\u003e with high purity and solubility. It delivers powerful inhibition against a wide range of RNases (RNase A, B, and C), and is designed for optimal performance in RT-PCR and qPCR systems.\u003c\/p\u003e\n\u003ch2 data-start=\"672\" data-end=\"695\"\u003eKey Features\u003c\/h2\u003e\n\u003cul data-start=\"697\" data-end=\"1335\"\u003e\n\u003cli data-start=\"697\" data-end=\"870\" class=\"\"\u003e\n\u003cp data-start=\"699\" data-end=\"870\" class=\"\"\u003e\u003cstrong data-start=\"699\" data-end=\"729\"\u003eOxidation-Resistant Design\u003c\/strong\u003e\u003cbr data-start=\"729\" data-end=\"732\"\u003eLacks two cysteines found in human RNase inhibitors, offering \u003cstrong data-start=\"796\" data-end=\"830\"\u003esuperior antioxidant stability\u003c\/strong\u003e—ideal for oxidation-prone environments.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"872\" data-end=\"1009\" class=\"\"\u003e\n\u003cp data-start=\"874\" data-end=\"1009\" class=\"\"\u003e\u003cstrong data-start=\"874\" data-end=\"909\"\u003eDTT-Sensitive System Compatible\u003c\/strong\u003e\u003cbr data-start=\"909\" data-end=\"912\"\u003eExcellent for \u003cstrong data-start=\"928\" data-end=\"948\"\u003eqPCR and RT-qPCR\u003c\/strong\u003e workflows where high DTT concentrations can disrupt results.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1011\" data-end=\"1159\" class=\"\"\u003e\n\u003cp data-start=\"1013\" data-end=\"1159\" class=\"\"\u003e\u003cstrong data-start=\"1013\" data-end=\"1040\"\u003eUltra-Pure \u0026amp; RNase-Free\u003c\/strong\u003e\u003cbr data-start=\"1040\" data-end=\"1043\"\u003eExpressed in \u003cem data-start=\"1058\" data-end=\"1067\"\u003eE. coli\u003c\/em\u003e for \u003cstrong data-start=\"1072\" data-end=\"1090\"\u003emaximum purity\u003c\/strong\u003e and \u003cstrong data-start=\"1095\" data-end=\"1115\"\u003eno RNase residue\u003c\/strong\u003e, ensuring consistent, high-quality results.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1161\" data-end=\"1335\" class=\"\"\u003e\n\u003cp data-start=\"1163\" data-end=\"1213\" class=\"\"\u003e\u003cstrong data-start=\"1163\" data-end=\"1186\"\u003eBroad Compatibility\u003c\/strong\u003e\u003cbr data-start=\"1186\" data-end=\"1189\"\u003eWorks seamlessly with:\u003c\/p\u003e\n\u003cul data-start=\"1216\" data-end=\"1335\"\u003e\n\u003cli data-start=\"1216\" data-end=\"1254\" class=\"\"\u003e\n\u003cp data-start=\"1218\" data-end=\"1254\" class=\"\"\u003eHiScript® II Reverse Transcriptase\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1257\" data-end=\"1292\" class=\"\"\u003e\n\u003cp data-start=\"1259\" data-end=\"1292\" class=\"\"\u003eHiScript® Reverse Transcriptase\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1295\" data-end=\"1315\" class=\"\"\u003e\n\u003cp data-start=\"1297\" data-end=\"1315\" class=\"\"\u003eM-MLV (RNase H–)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1318\" data-end=\"1335\" class=\"\"\u003e\n\u003cp data-start=\"1320\" data-end=\"1335\" class=\"\"\u003eDNA Polymerases\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 data-start=\"1342\" data-end=\"1365\"\u003eApplication\u003c\/h2\u003e\n\u003cul data-start=\"1367\" data-end=\"1462\"\u003e\n\u003cli data-start=\"1367\" data-end=\"1387\" class=\"\"\u003e\n\u003cp data-start=\"1369\" data-end=\"1387\" class=\"\"\u003eRT-PCR \u0026amp; RT-qPCR\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1388\" data-end=\"1424\" class=\"\"\u003e\n\u003cp data-start=\"1390\" data-end=\"1424\" class=\"\"\u003eRNA Protection in cDNA Synthesis\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1425\" data-end=\"1462\" class=\"\"\u003e\n\u003cp data-start=\"1427\" data-end=\"1462\" class=\"\"\u003eSensitive RNA Workflow Optimization\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 data-start=\"1469\" data-end=\"1517\"\u003eWhy Choose Our Murine RNase Inhibitor?\u003c\/h2\u003e\n\u003cul data-start=\"1519\" data-end=\"1750\"\u003e\n\u003cli data-start=\"1519\" data-end=\"1566\" class=\"\"\u003e\n\u003cp data-start=\"1521\" data-end=\"1566\" class=\"\"\u003eEnhanced performance under oxidative stress\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1567\" data-end=\"1622\" class=\"\"\u003e\n\u003cp data-start=\"1569\" data-end=\"1622\" class=\"\"\u003eHigher stability compared to human RNase inhibitors\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1623\" data-end=\"1698\" class=\"\"\u003e\n\u003cp data-start=\"1625\" data-end=\"1698\" class=\"\"\u003eExtensively tested for compatibility with reverse transcription systems\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1699\" data-end=\"1750\" class=\"\"\u003e\n\u003cp data-start=\"1701\" data-end=\"1750\" class=\"\"\u003eEasy integration into your existing lab protocols\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eSee manual for more details on R301:\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/R301_Manual_GBS-April_2025.pdf?v=1745336637\" target=\"_blank\" rel=\"noopener\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\" width=\"188\" height=\"53\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eStore at -20℃\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"2000 U","offer_id":39771531608164,"sku":"R301-01","price":83.46,"currency_code":"CAD","in_stock":true},{"title":"10,000 U","offer_id":39771531640932,"sku":"R301-02","price":383.44,"currency_code":"CAD","in_stock":true},{"title":"20,000 U","offer_id":39771531673700,"sku":"R301-03","price":664.07,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/Murine.jpg?v=1702052280"},{"product_id":"hyperactive-pg-mnase-cut-run-assay-kit-for-pcr-qpcr","title":"Hyperactive pG-MNase CUT\u0026RUN Assay Kit for PCR\/qPCR","description":"\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e Hyperactive pG-MNase CUT\u0026amp;RUN Assay Kit for PCR\/qPCR is developed specially for studying protein-DNA interactions. Cleavage Under Targets and Release Using Nuclease (CUT\u0026amp;RUN) is a new method for studying protein-DNA interactions. By fusing Protein G with transposase, under the guidance of the antibody, it allows accurate targeting of the target protein and cutting of the DNA sequence near the target site. With an optimized reaction system and workflow, this kit offers multiple advantages over conventional ChIP-qPCR, including high success rate, excellent antibody compatibility, short turnaround time, and ease of use, making It is especially suitable for such fields as early embryonic development, stem cells, cancer, and epigenetics. All reagents in this kit have undergone strict quality control and function testing to ensure the optimal stability and repeatability of experimental results.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eApplications\u003cbr\u003e06-1\/About qPCR Reagents\u003cbr\u003eThis product is intended for studying protein-DNA interactions in mammalian cells with\u003cbr\u003e5,000 - 500,000 input cells. Yeast, plant tissues and cells should be treated before proceeding\u003cbr\u003ewith the assay using this kit.\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"24 rxn","offer_id":41740635570276,"sku":"HD101-01","price":1003.87,"currency_code":"CAD","in_stock":true},{"title":"48 rxn","offer_id":41740635603044,"sku":"HD101-02","price":1743.57,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/hd101image.png?v=1683497614"},{"product_id":"transscript-r-all-in-one-first-strand-cdna-synthesis-supermix-for-qpcr-one-step-gdna-removal","title":"TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal )","description":"\u003cdiv class=\"col-sm-12 col-md-6 proinfo_box\"\u003e\n\u003cp\u003e\u003cem style=\"font-size: 0.875rem;\"\u003eTransScript\u003c\/em\u003e\u003csup\u003e®\u003c\/sup\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal )-AT341\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-sm-12 col-md-6 proinfo_box\"\u003e\n\u003cul class=\"product_info product_attr\"\u003e\n\u003cul class=\"product_info product_attr\"\u003e\n\u003cli class=\"item_group\"\u003eSpecification：\u003c\/li\u003e\n\u003cli class=\"guige2 item item_selected\" value=\"1\"\u003e\u003ca\u003e50 rxns×20 µl system\u003c\/a\u003e\u003c\/li\u003e\n\u003cli class=\"guige2 item\" value=\"2\"\u003e\u003ca\u003e100 rxns×20 µl system\u003c\/a\u003e\u003c\/li\u003e\n\u003cli class=\"guige2 item\" value=\"3\"\u003e\u003ca\u003e500 rxns×20 µl system\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003ch2 class=\"col-xs-12 col-sm-12 col-md-12 col-lg-12 acontent\"\u003e\u003cspan class=\"author\"\u003e\u003cstrong\u003eProduct Details\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp class=\"col-xs-12 col-sm-12 col-md-12 col-lg-12 acontent\"\u003eThe mastermix provides all the necessary components for cDNA synthesis from total RNA or mRNA. It is provided at 5× concentration and used at 1× concentration by adding gDNA remover, RNA and H2O. Simultaneous genomic DNA removal and cDNA synthesis are performed. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85℃ for 5 seconds. \u003cstrong\u003eThe resulting cDNA is suitable for qPCR \u003cspan style=\"color: rgb(255, 42, 0);\"\u003e\u003cem\u003e\u003cspan style=\"text-decoration: underline;\"\u003e(\u003c\/span\u003e\u003c\/em\u003e\u003cem\u003e\u003cspan style=\"text-decoration: underline;\"\u003enot \u003c\/span\u003e\u003c\/em\u003efor regular end-point PCR). qPCR mastermix is not included with product.\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cdiv class=\"col-xs-12 col-sm-12 col-md-12 col-lg-12 acontent\"\u003e\n\u003cdiv class=\"acontentc\"\u003e\n\u003cul\u003e\n\u003cli\u003eSimultaneous genomic DNA removal and cDNA synthesis. \u003c\/li\u003e\n\u003cli\u003eThe optimal ratio of oligo(dT)18 primer to random primer(N9) for qPCR ready cDNA.\u003c\/li\u003e\n\u003cli\u003eqPCR ready cDNA in 15 minutes.\u003c\/li\u003e\n\u003cli\u003ecDNA up to 250 bp.\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis mastermix synthesizes first-strand cDNA for qPCR, it does not contain the components for a qPCR reaction. \u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.genebiosystems.com\/products\/perfectstart%C2%AE-universal-green-qpcr-supermix\" rel=\"noopener\" target=\"_blank\"\u003e\u003cspan style=\"color: rgb(43, 0, 255);\"\u003ePerfectStart® Universal Green qPCR SuperMix (Cat.#AQ602)\u003c\/span\u003e \u003c\/a\u003esold separately from AT341.\u003c\/p\u003e\n\u003cp\u003eFor a true one-step cDNA synthesis \u003cstrong\u003eand\u003c\/strong\u003e qPCR supermix (\u003cstrong\u003eqRT-PCR\u003c\/strong\u003e), Please see our\u003cspan style=\"color: rgb(43, 0, 255);\"\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.genebiosystems.com\/products\/transscript%C2%AE-ii-green-one-step-qrt-pcr-supermix\" target=\"_blank\"\u003e\u003cspan style=\"color: rgb(43, 0, 255);\"\u003eTransScript® II Green One-Step qRT-PCR SuperMix (Cat.#AQ311)\u003c\/span\u003e\u003c\/a\u003e.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eSee manual for more details on AT341:\u003c\/p\u003e\n\u003cp\u003e\u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/AT341_GBS-Manual_Feb_2025.pdf?v=1740148695\" target=\"_blank\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\" width=\"188\" height=\"53\"\u003e\u003c\/a\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/AT341_GBS-Manual_Feb_2025.pdf?v=1740148695\" rel=\"noopener\" target=\"_blank\"\u003e\u003c\/a\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/AQ311_GBS-Nov2024.pdf?v=1731435128\" rel=\"noopener\" target=\"_blank\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eMultiple copy and low copy gene detection\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eat -20 ℃\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003e\n\u003cstrong\u003eShipping\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eIce\/Gel packs (4℃)\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"acontentc\"\u003e\n\u003cdiv class=\"clear\"\u003e\n\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/AT341_2023-12-29-GBS.pdf?v=1705431644\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/a\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2\u003e\n\u003cstrong\u003eProduct contents\u003c\/strong\u003e\u003cspan class=\"author\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003cspan class=\"author\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cbr\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-xs-12 col-sm-12 col-md-12 col-lg-12 acontent\"\u003e\n\u003cdiv class=\"acontentc\"\u003e\n\u003ctable border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr class=\"firstRow\"\u003e\n\u003ctd\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003eAT341-01 (50 rxns )\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003eAT341-02 (100 rxns )\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003eAT341-03 (500 rxns )\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e5×\u003cem\u003eTransScript\u003c\/em\u003e\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eAll-in-One SuperMix for qPCR\u003c\/td\u003e\n\u003ctd\u003e200 μl\u003c\/td\u003e\n\u003ctd\u003e400 μl\u003c\/td\u003e\n\u003ctd\u003e5×400 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e5×\u003cem\u003eTransScript\u003c\/em\u003e\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eAll-in-One No-RT Control SuperMix for qPCR\u003c\/td\u003e\n\u003ctd\u003e20 μl\u003c\/td\u003e\n\u003ctd\u003e40 μl\u003c\/td\u003e\n\u003ctd\u003e200 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003egDNA Remover\u003c\/td\u003e\n\u003ctd\u003e50 μl\u003c\/td\u003e\n\u003ctd\u003e100 μl\u003c\/td\u003e\n\u003ctd\u003e5×100 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase-free Water\u003c\/td\u003e\n\u003ctd\u003e1 ml\u003c\/td\u003e\n\u003ctd\u003e2×1 ml\u003c\/td\u003e\n\u003ctd\u003e2×5 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"clear\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2\u003e\u003cstrong\u003eCitations\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cspan class=\"author\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003cspan class=\"author\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-xs-12 col-sm-12 col-md-12 col-lg-12 acontent\"\u003e\n\u003cdiv class=\"acontentc\"\u003e\n\u003cp\u003e\u003cspan\u003eHan X , Wang R , Zhou Y , \u003cem\u003eet al\u003c\/em\u003e. Mapping the Mouse Cell Atlas by Microwell-Seq[J]. Cell, 2018, 172(5):1091-1107.e17.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eTian C , Zhang X , He J , et al. An organ boundary-enriched gene regulatory network uncovers regulatory hierarchies underlying axillary meristem initiation[J]. Molecular Systems Biology, 2014, 10(10):755-755.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eWang J , Zhang Y , Hou J , \u003cem\u003eet al\u003c\/em\u003e. Ube2s regulates Sox2 stability and mouse ES cell maintenance[J]. Cell death and differentiation, 2015, 23(2).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eZhang L , Zhao X , Zhang G , \u003cem\u003eet \u003c\/em\u003e\u003cem\u003eal\u003c\/em\u003e. Light-inducible genetic engineering and control of non-homologous end-joining in industrial eukaryotic microorganisms: LML 3.0 and OFN 1.0[J]. Scientific Reports, 2016, 6:20761.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eLi P , Shi M L , Shen W L , \u003cem\u003eet al\u003c\/em\u003e. Coordinated regulation of IFITM1, 2 and 3 genes by an IFN-responsive enhancer through long-range chromatin interactions[J]. Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 2017, 1860(8):885-893.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Gene Bio Systems","offers":[{"title":"50 reactions","offer_id":42447248064612,"sku":"AT341-01","price":410.6,"currency_code":"CAD","in_stock":true},{"title":"100 reactions","offer_id":42447248097380,"sku":"AT341-02","price":700.36,"currency_code":"CAD","in_stock":true},{"title":"500 reactions","offer_id":42447255339108,"sku":"AT341-03","price":3022.79,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/20250320_145354.jpg?v=1742823606"},{"product_id":"transscript-r-ii-green-one-step-qrt-pcr-supermix","title":"TransScript®  II Green One-Step qRT-PCR SuperMix","description":"\u003cdiv class=\"col-sm-12 col-md-6 proinfo_box\"\u003e\n\u003cdiv class=\"acontentc\"\u003e\n\u003cp\u003e\u003cem\u003eTransScript\u003c\/em\u003e\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eII Green One-Step qRT-PCR SuperMix AQ311\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"fontstyle0\"\u003eis designed for one-step qRT-PCR with high sensitivity, high synthesis efficiency and high amplification efficiency. This kit firstly synthesizes first-strand cDNA with RNA as templates using reverse gene-specific primers, and then performs qPCR with the synthesized cDNA as templates using both forward and reverse gene-specific primers to achieve one step from reverse transcription to qPCR in a single tube.\u003c\/span\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eHighlights\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e•\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eTransScript\u003c\/em\u003e\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eII Green One-Step RT\/RI Enzyme Mix is used to efficiently synthesize first-strand cDNA from RNA and 2×\u003cem\u003ePerfectStart\u003c\/em\u003e\u003csup\u003eTM\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eGreen One-Step qPCR SuperMix is used for qPCR, enabling simple procedures to minimize the chance of contamination.\u003cbr\u003e•\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"fontstyle0\"\u003eHigh sensitivity and high specificity to ensure accurate results.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003cbr\u003e•\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"fontstyle0\"\u003eHigh-copy and low-copy gene detection\u003cbr\u003e• Detection of RNA virus or trace amounts of RNA\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003cbr\u003eat -20°C\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"fontstyle0\"\u003eaway from light\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eShipping\u003c\/strong\u003e\u003cbr\u003eIce\/Gel Packs (~2-8℃)\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"acontentc\"\u003e\n\u003cdiv class=\"clear\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cspan class=\"author\"\u003e\u003cstrong\u003eProduct Contents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"acontentc\"\u003e\n\u003ctable border=\"1\" style=\"width: 632px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 323.761px;\"\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 96.2273px;\"\u003e\u003cstrong\u003e100rxn x 20ul\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 96.2273px;\"\u003e\u003cstrong\u003e400rxn x 20ul\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 93.2386px;\"\u003e\u003cstrong\u003e1000rxn x 20ul\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"firstRow\"\u003e\n\u003ctd style=\"width: 323.761px;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 96.2273px;\"\u003e\u003cstrong\u003eAQ311-01\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 96.2273px;\"\u003e\u003cstrong\u003eAQ311-02\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 93.2386px;\"\u003e\u003cstrong\u003eAQ311-03\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 323.761px;\"\u003e\n\u003cem\u003eTransScript\u003c\/em\u003e\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eII Green One-Step RT\/ RI Enzyme Mix\u003c\/td\u003e\n\u003ctd style=\"width: 96.2273px;\"\u003e40 μl\u003c\/td\u003e\n\u003ctd style=\"width: 96.2273px;\"\u003e160 μl\u003c\/td\u003e\n\u003ctd style=\"width: 93.2386px;\"\u003e400 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 323.761px;\"\u003e\n\u003cem\u003ePerfectStart\u003c\/em\u003e\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eGreen One-Step qPCR SuperMix (2×)\u003c\/td\u003e\n\u003ctd style=\"width: 96.2273px;\"\u003e1 ml\u003c\/td\u003e\n\u003ctd style=\"width: 96.2273px;\"\u003e4×1 ml\u003c\/td\u003e\n\u003ctd style=\"width: 93.2386px;\"\u003e10×1 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 323.761px;\"\u003ePassive Reference Dye (50×)\u003c\/td\u003e\n\u003ctd style=\"width: 96.2273px;\"\u003e40 μl\u003c\/td\u003e\n\u003ctd style=\"width: 96.2273px;\"\u003e160 μl\u003c\/td\u003e\n\u003ctd style=\"width: 93.2386px;\"\u003e400 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 323.761px;\"\u003eRNase-free Water\u003c\/td\u003e\n\u003ctd style=\"width: 96.2273px;\"\u003e1 ml\u003c\/td\u003e\n\u003ctd style=\"width: 96.2273px;\"\u003e4×1 ml\u003c\/td\u003e\n\u003ctd style=\"width: 93.2386px;\"\u003e10×1 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cdiv class=\"clear\"\u003e\n\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/AQ311_GBS-Nov2024.pdf?v=1731435128\" rel=\"noopener\" target=\"_blank\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\" width=\"188\" height=\"53\"\u003e\u003c\/a\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/AQ311_GBS-Nikita.pdf?v=1722255502\" rel=\"noopener\" target=\"_blank\"\u003e\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cspan class=\"author\"\u003e\u003cstrong\u003eCitations\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"acontentc\"\u003e\n\u003cp\u003e\u003cspan\u003eSynaptotagmin-11 inhibits cytokine secretion and phagocytosis in microglia[J]. Glia, 2017, 65(10):1656-1667. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA differential sequencing-based analysis of the C. elegans noncoding transcriptome[J]. RNA, 2012, 18(4):626-639.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e Regulatory elements of Caenorhabditis elegans ribosomal protein genes[J]. BMC Genomics, 2012, 28(13):433.4.The \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eloss of MiR-139-5p promotes colitis-associated tumorigenesis by mediating PI3K\/AKT\/Wnt signaling[J]. The International Journal of Biochemistry \u0026amp; Cell Biology, 2015, 69:153-161.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e Identification of Intermediate-Size Non-Coding RNAs Involved in the UV-Induced DNA Damage Response in C. elegans[J]. PLoS ONE, 2012, 7(11):e48066.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-xs-12 col-sm-12 col-md-12 col-lg-12 acontent\"\u003e\n\u003cdiv class=\"acontentc\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Gene Bio Systems","offers":[{"title":"100 reactions","offer_id":43192506679396,"sku":"AQ311-01","price":301.19,"currency_code":"CAD","in_stock":true},{"title":"400 reactions","offer_id":43192506712164,"sku":"AQ311-02","price":845.51,"currency_code":"CAD","in_stock":true},{"title":"1200 reactions","offer_id":43192542462052,"sku":"AQ311-03","price":2170.02,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/20250320_150449.jpg?v=1742823663"},{"product_id":"gb-script-ts-1st-strand-cdna-synthesis-kit-gdna-wiper","title":"GB-Script™-TS 1st Strand cDNA Synthesis Kit (+ gDNA wiper)","description":"\u003cdiv class=\"acontentc\"\u003e\n\u003cp\u003eUnique genomic DNA remover is combined with\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eTransScript\u003c\/em\u003e\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eFirst-Strand cDNA Synthesis kit (AT311) to enable simultaneous genomic DNA removal and cDNA synthesis. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85℃ for 5 seconds.\u003cbr\u003eThe users can optimize the reverse transcription primer, choosing between polydT and random 9-mers, as well as the primer concentration. In addition, the concentration of the transcriptase can also be optimized. \u003c\/p\u003e\n\u003cp\u003e• Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.\u003cbr\u003e• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.\u003cbr\u003e• cDNA up to 12 kb.\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003cbr\u003eMultiple copy and low copy gene detection\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003cbr\u003eat -20 ℃ for two years\u003cbr\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"acontentc\"\u003e\n\u003cdiv class=\"clear\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cspan class=\"author\"\u003e\u003cstrong\u003eProduct Contents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"acontentc\"\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ctable border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr class=\"firstRow\"\u003e\n\u003ctd\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003eAT311-02 (50 rxns)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003eAT311-03 (100 rxns)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003eAT311-04 (500 rxns)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eTransScript\u003c\/em\u003e\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eRT\/RI Enzyme Mix\u003c\/td\u003e\n\u003ctd\u003e50 μl\u003c\/td\u003e\n\u003ctd\u003e100 μl\u003c\/td\u003e\n\u003ctd\u003e5×100 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003egDNA Remover\u003c\/td\u003e\n\u003ctd\u003e50 μl\u003c\/td\u003e\n\u003ctd\u003e100 μl\u003c\/td\u003e\n\u003ctd\u003e5×100 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2×TS Reaction Mix\u003c\/td\u003e\n\u003ctd\u003e500 μl\u003c\/td\u003e\n\u003ctd\u003e1 ml\u003c\/td\u003e\n\u003ctd\u003e5×1 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRandom Primer(N9) (0.1 μg\/μl)\u003c\/td\u003e\n\u003ctd\u003e50 μl\u003c\/td\u003e\n\u003ctd\u003e100 μl\u003c\/td\u003e\n\u003ctd\u003e5×100 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAnchored Oligo(dT)\u003csub\u003e18\u003c\/sub\u003e\u003cspan\u003e \u003c\/span\u003ePrimer (0.5 μg\/μl)\u003c\/td\u003e\n\u003ctd\u003e50 μl\u003c\/td\u003e\n\u003ctd\u003e100 μl\u003c\/td\u003e\n\u003ctd\u003e5×100 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase-free Water\u003c\/td\u003e\n\u003ctd\u003e500 μl\u003c\/td\u003e\n\u003ctd\u003e1 ml\u003c\/td\u003e\n\u003ctd\u003e5 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cdiv class=\"clear\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cspan class=\"author\"\u003e\u003cstrong\u003eCitations\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"acontentc\"\u003e\n\u003cp\u003e\u003cspan\u003eChen J , Ou Y , Yang Y ,\u003cem\u003e et al\u003c\/em\u003e. KLHL22 activates amino-acid-dependent mTORC1 signalling to promote tumorigenesis and ageing[J]. Nature, 2018,557(7706):585-589.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eYang Y , Liu B , Xu J , \u003cem\u003eet al\u003c\/em\u003e. Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency[J]. Cell, 2017, 169(2):243-257.e25.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eWu D , Qi T , Li W X , \u003cem\u003eet al\u003c\/em\u003e. Viral effector protein manipulates host hormone signaling to attract insect vectors[J]. Cell Research, 2017, 27(3):402-415.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eShao X , Liu Y , Yu Q , \u003cem\u003eet al\u003c\/em\u003e. Numb regulates vesicular docking for homotypic fusion of early endosomes via membrane recruitment of Mon1b[J]. Cell Research, 2016,26(5):593-612.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eMa D , Hu Y , Yang C , \u003cem\u003eet al\u003c\/em\u003e. Genetic basis for glandular trichome formation in cotton[J]. Nature Communications, 2016, 7:10456.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/AT311-GBS-Manual-Oct-2024.pdf?v=1731435967\" title=\"AT311 User Manual\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"228\" height=\"64\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"50 reactions","offer_id":44103408451684,"sku":"AT311-02","price":325.38,"currency_code":"CAD","in_stock":true},{"title":"100 reactions","offer_id":44103408484452,"sku":"AT311-03","price":603.59,"currency_code":"CAD","in_stock":true},{"title":"500 reactions","offer_id":44103419363428,"sku":"AT311-04","price":2055.11,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/at311_image.jpg?v=1742220844"},{"product_id":"rnase-inhibitor-pro","title":"RNase Inhibitor Pro","description":"\u003cdiv data-anchor=\"introduce\" class=\"page-title anchor\"\u003e\n\u003ch3\u003eProduct Details\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product-brief\"\u003e\n\u003cp\u003eRNase Inhibitor Pro is a recombinant protein purified from E. coli strain carrying porcine ribonuclease inhibitor gene. It can specifically inhibits RNase A, RNase B, and RNase C, and not effective against RNase 1, RNase T1, S1 nuclease, RNase H and aspergillus-originated RNase. It has no inhibition effect on DNA Polymerase, AMV RTase, M-MLV RTase, SP6, T7 and T3 RNA Polymerases.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eApplications \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eFirst-strand cDNA synthesis, isolation of polysomes, in vitro translation, in vitro transcription by cell-free systems, in vitro transcription of SP6 or T7 RNA polymerases.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eat -18℃ or below for two years\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eDry ice (-70℃)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"region-title\"\u003eProduct Composition\u003c\/div\u003e\n\u003cdiv class=\"text\"\u003e\n\u003ctable border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr class=\"firstRow\"\u003e\n\u003ctd\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e    AI201-01\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"null\" rowspan=\"1\" colspan=\"1\"\u003e\u003cstrong\u003e       AI201-02\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"92\" valign=\"null\" rowspan=\"1\" colspan=\"1\"\u003e\u003cstrong\u003e   AI201-03\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase Inhibitor Pro\u003c\/td\u003e\n\u003ctd\u003e  2000 units\u003c\/td\u003e\n\u003ctd valign=\"null\" rowspan=\"1\" colspan=\"1\"\u003e  5×2000 units\u003c\/td\u003e\n\u003ctd width=\"92\" valign=\"null\" rowspan=\"1\" colspan=\"1\"\u003e20000 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Gene Bio Systems","offers":[{"title":"2000 units","offer_id":46787562995812,"sku":"AI201-01","price":72.8,"currency_code":"CAD","in_stock":true}]},{"product_id":"transscript-r-ii-multiplex-probe-one-step-qrt-pcr-supermix-udg","title":"TransScript® II Multiplex Probe One-Step qRT-PCR SuperMix UDG","description":"\u003cdiv class=\"page-title anchor\" data-anchor=\"introduce\"\u003e\n\u003ch3\u003eProduct Details\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product-brief\"\u003e\n\u003cp\u003e\u003cspan\u003e\u003cem\u003eTransScript\u003c\/em\u003e\u003csup\u003e®\u003c\/sup\u003e II Multiplex Probe One-Step qRT-PCR SuperMix UDG is designed for one-step qRT-PCR with high sensitivity, high synthesis efficiency and high amplification efficiency. This kit firstly synthesizes first-strand cDNA with RNA as templates using reverse gene-specific primers, and then performs qPCR with the synthesized cDNA as templates using both forward and reverse gene-specific primers and fluorescent probes to achieve one step from reverse transcription to qPCR in a single tube. After special optimization, this kit enables consistently amplifying multiple targets in a single reaction ensuring multiplex one-step RT-qPCR. dUTP\/ UDG is included in the kit to degrade dU-containing ssDNA and dsDNA, which can prevent cross contamination.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eHighlights\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Use \u003cem\u003eT\u003c\/em\u003e\u003cem\u003eransScript\u003c\/em\u003e\u003csup\u003e®\u003c\/sup\u003e II Multiplex Probe One-Step Enzyme Mix UDG, 2×\u003cem\u003ePerfectStart\u003c\/em\u003e\u003csup\u003e®\u003c\/sup\u003e Multiplex Probe One-Step Reaction Mix to efficiently synthesize RNA into first-strand cDNA for qPCR. It is easy to operate and reduces the chance of contamination during operation. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Using UDG enzyme and dUTP to effectively prevent cross-contamination of PCR products with accurate data. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• High sensitivity, high specificity and the data are accurate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Singleplex to 4-plex RT-qPCR detection.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• High-copy and low-copy gene detection.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• RNA templates with high GC content or complex secondary structure.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Detection of RNA virus or trace amounts of RNA.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStore at -20°C for two years\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eShipping\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eDry ice (-70 ℃)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"region-title\"\u003eProduct Composition\u003c\/div\u003e\n\u003cdiv class=\"text\"\u003e\n\u003ctable border=\"1\" width=\"-103\"\u003e\n\u003ctbody\u003e\n\u003ctr class=\"firstRow\"\u003e\n\u003ctd\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003eAQ322-01\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003eAQ322-02\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eTransScript\u003c\/em\u003e\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eII Multiplex Probe One-Step Enzyme Mix UDG\u003csup\u003e\u003cbr\u003e\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd\u003e80 μl\u003c\/td\u003e\n\u003ctd\u003e320 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2×\u003cem\u003ePerfectStart\u003c\/em\u003e\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eMultiplex Probe One-Step Reaction Mix\u003c\/td\u003e\n\u003ctd\u003e1 ml\u003c\/td\u003e\n\u003ctd\u003e4×1 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePassive Reference Dye (50×)\u003c\/td\u003e\n\u003ctd\u003e40 μl\u003c\/td\u003e\n\u003ctd\u003e160 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNuclease-free Water\u003c\/td\u003e\n\u003ctd\u003e1 ml\u003c\/td\u003e\n\u003ctd\u003e4×1 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Gene Bio Systems","offers":[{"title":"100 rxns×20 μl system","offer_id":46787575185508,"sku":"AQ322-01","price":334.88,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_6681fc76-aaa2-4a4e-9cf9-cdaf4fdfac5a.jpg?v=1756484266"},{"product_id":"hiscript-iv-rt-supermix-for-qpcr-gdna-wiper","title":"HiScript IV RT SuperMix for qPCR (+gDNA wiper)","description":"\u003cp\u003e\u003cstrong\u003eSize: 100 rxns \u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"tabs6 el-tabs el-tabs--top\"\u003e\n\u003cdiv class=\"el-tabs__content\"\u003e\n\u003cdiv class=\"el-tab-pane\" aria-labelledby=\"tab-aba8736c-1392-424d-93d3-7590b8b765b9\" id=\"pane-aba8736c-1392-424d-93d3-7590b8b765b9\" role=\"tabpanel\"\u003e\n\u003cdiv class=\"article-box\" id=\"contentHtml\"\u003e\n\u003cdiv\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description  \u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eHiScript IV RT SuperMix for qPCR (+gDNA wiper) is an upgraded version of HiScript III RT SuperMix for qPCR (+gDNA wiper), including a new generation of HiScript IV Reverse Transcriptase and Buffer optimized for reverse transcription. This kit further improves the synthesis efficiency of cDNA, making it a better choice for reverse transcription of low-input, low-expression or degraded RNA templates. 5 × gDNA wiper Mix can completely remove the genomic contaimination in the RNA template, so there is no need to design intron-spanning qPCR primers. 4 × HiScript IV qRT SuperMix contains all the components required for the reverse transcription, just add template RNA and RNase-free ddH\u003csub\u003e2\u003c\/sub\u003eO to start the reaction.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e Features\u003c\/strong\u003e  \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul class=\"list-paddingleft-2\"\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eSuperior reverse transcription efficiency: Achieve qPCR detection results with smaller \u003c\/span\u003e\u003cspan\u003eC\u003csub\u003eT\u003c\/sub\u003e\u003c\/span\u003e\u003cspan\u003e values. \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eGreat gDNA elimination efficiency: Thoroughly remove 500 ng of gDNA to ensure the accuracy of qPCR detection. \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eExcellent template compatibility: Efficient reverse transcription of low-input, low-expression or degraded RNA templates.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e Components  \u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"HiScript IV RT SuperMix for qPCR (+gDNA wiper)\" src=\"https:\/\/vazyme-singapore-website-prod.s3.ap-southeast-1.amazonaws.com\/a1f9b8a9a3f74e5498c8e68196135a2f\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003cstrong\u003eStorage  \u003c\/strong\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eStore at -30 ~ -15°C and transport at ≤0°C.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Performance  \u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eFaster Reaction\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg height=\"185\" width=\"967\" alt=\"HiScript IV RT SuperMix for qPCR (+gDNA wiper)\" src=\"https:\/\/vazyme-singapore-website-prod.s3.ap-southeast-1.amazonaws.com\/1d5d6f73c623439ea3a0612682e7854c\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eSuperior RT Efficiency\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e100 ng RNA from animals, plants and microbes were reverse transcribed by Vazyme #R423 and similar RT products from Supplier A,  Supplier B and Supplier C, following their respective manuals. The RT efficiency of these reagents was evaluated by qPCR amplification of obtained cDNA. It can be seen from the figure, △CT between Vazyme #R423 and Supplier C is within 0.5, indicating a  similar efficiency of them; △C\u003csub\u003eT\u003c\/sub\u003e between Vazyme #R423 and Suppler A\/Supplier B are more than 0.5, indicating a better efficiency of Vazyme #R423.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg height=\"438\" width=\"843\" alt=\"HiScript IV RT SuperMix for qPCR (+gDNA wiper)\" src=\"https:\/\/vazyme-singapore-website-prod.s3.ap-southeast-1.amazonaws.com\/617833e697e44ff5a84ae8ed3b4be33a\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eG\u003c\/strong\u003e\u003c\/span\u003e\u003cspan\u003e\u003cstrong\u003ereat gDNA Elimination Efficiency\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e500 ng gDNA from 293T cells were eliminated by Vazyme #R423 and similar RT products from Supplier A, Supplier B and  Supplier C. The digested gDNA were amplified by qPCR to evaluate the elimination efficiency of these products. The results  show that the gDNA elimination efficiency of Vazyme #R423 is consistent with that of Supplier A and Supplier B, and significantly  better than that of Supplier C.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg height=\"311\" width=\"981\" alt=\"HiScript IV RT SuperMix for qPCR (+gDNA wiper)\" src=\"https:\/\/vazyme-singapore-website-prod.s3.ap-southeast-1.amazonaws.com\/ef27d6cd477542029966fbf0d5e22ff3\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eExcellent Template Compatibility\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e100 ng human FFPE samples and degraded 293T cells (Rin=1) were reverse transcribed by Vazyme #R423 and similar RT  products from Supplier A, Supplier B and Supplier C. The template compatibility of these reagents was evaluated by qPCR amplification of obtained cDNA. The results show that △CT between Vazyme #R423 and Suppliers are more than 0.5 in most genes, indicating a better template compatibility of Vazyme #R423.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg height=\"403\" width=\"921\" alt=\"HiScript IV RT SuperMix for qPCR (+gDNA wiper)\" src=\"https:\/\/vazyme-singapore-website-prod.s3.ap-southeast-1.amazonaws.com\/2259dab1b2b44563a987a4895bfef55c\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":47172647878756,"sku":"R423-01","price":738.08,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/Screenshot_136.png?v=1761319464"}],"url":"https:\/\/www.genebiosystems.com\/en-us\/collections\/reverse-transcription\/reverse-transcription.oembed?page=2","provider":"GeneBio ","version":"1.0","type":"link"}