{"product_id":"solpure-hw-dna-kit","title":"SolPure High Molecular Weight DNA kit","description":"\u003carticle id=\"i1\" class=\"info\"\u003e\n\u003cdiv class=\"title\"\u003e\n\u003ch3\u003e\u003cspan\u003eIntroduction\u003c\/span\u003e\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThis product uses an improved salt precipitation purification method to provide a safe and economical solution for \u003cstrong\u003eHigh Weight genomic DNA\u003c\/strong\u003e extraction from blood samples, tissue samples, cultured cells, oral swabs, bacteria, and other samples. The extraction does not require the use of toxic phenol chloroform or any expensive reagents, making it the most economical reagent kit for nucleic acid extraction at present. This kit has no limit on the amount of sample used and can flexibly adjust various amounts of samples. The obtained DNA can be directly used for experiments such as PCR, enzyme digestion, Southern hybridization, and the Third-generation sequencing.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/article\u003e\n\u003carticle id=\"i2\" class=\"info\"\u003e\n\u003cdiv class=\"title\"\u003e\n\u003ch3\u003e\u003cspan\u003eDetails\u003c\/span\u003e\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003ePrinciples\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eSolPure DNA Kits is an improved salt precipitation purification method. (Blood samples are lysed in red blood cell lysis buffer to remove red blood cells, and white blood cells are collected by centrifugation.) After lysis, DNA is released into the lysis buffer. RNA is removed by RNASE A. High salt solution is added to precipitate proteins and impurities. Centrifuge to remove precipitate and obtain supernatant containing only DNA. Add isopropanol to precipitate and recover DNA. Wash with 70% ethanol to remove salt, and finally add Buffer TE to dissolve DNA.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"box\"\u003e\n\u003cdiv class=\"sub_title\"\u003e\n\u003ch1\u003e\u003cspan\u003eKit Contents\u003c\/span\u003e\u003c\/h1\u003e\n\u003c\/div\u003e\n\u003ctable width=\"1185\"\u003e\n\u003ctbody\u003e\n\u003ctr class=\"firstRow\"\u003e\n\u003ctd\u003e\n\u003cspan\u003eC\u003c\/span\u003e\u003cspan\u003eon\u003c\/span\u003e\u003cspan\u003et\u003c\/span\u003e\u003cspan\u003eent\u003c\/span\u003e\u003cspan\u003es\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eD3317-01\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd valign=\"null\" rowspan=\"1\" colspan=\"1\"\u003e\u003cspan\u003eD3317-02\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003eD3317-03\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cspan\u003ePurifi\u003c\/span\u003e\u003cspan\u003ecation Times\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd valign=\"null\" rowspan=\"1\" colspan=\"1\"\u003e\u003cspan\u003e50\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e250\u003c\/span\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003e10 x Buffer RBC\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e4 ml\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd valign=\"null\" rowspan=\"1\" colspan=\"1\"\u003e\u003cspan\u003e50 ml\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e100 ml\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cspan\u003eBuffer STE\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e60 ml\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"null\" rowspan=\"1\" colspan=\"1\"\u003e\u003cspan\u003e250 ml\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e2 x 550 ml\u003c\/span\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eBuffer SDS (20%)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e6 ml\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"null\" rowspan=\"1\" colspan=\"1\"\u003e\u003cspan\u003e25 ml\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e100 ml\u003c\/span\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eBuffer PPS\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e20 ml\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"null\" rowspan=\"1\" colspan=\"1\"\u003e\u003cspan\u003e90 ml\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e400 ml\u003c\/span\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eProteinase K\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e12 mg\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"null\" rowspan=\"1\" colspan=\"1\"\u003e\u003cspan\u003e50 mg\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e240 mg\u003c\/span\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eProtease Dissolve Buffer\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e1.8 ml\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"null\" rowspan=\"1\" colspan=\"1\"\u003e\u003cspan\u003e10 ml\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e20 ml\u003c\/span\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eRNase A\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e5 mg\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"null\" rowspan=\"1\" colspan=\"1\"\u003e\u003cspan\u003e20 mg\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e60 mg\u003c\/span\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eBuffer TE\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e10 ml\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"null\" rowspan=\"1\" colspan=\"1\"\u003e\u003cspan\u003e60 ml\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e250 ml\u003c\/span\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch1\u003e\u003cb\u003e\u003cspan\u003eStorage and Stability\u003c\/span\u003e\u003c\/b\u003e\u003c\/h1\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eRNase A and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 4 weeks) at room temperature (15–25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least\u003c\/span\u003e\u003cb\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e18 months under these conditions. The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/article\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003ca title=\"User Manual for SolPure High Molecular Weight DNA kit\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/D3317_SolPure_HW_DNA_Kit_2024-GBS.pdf?v=1719067370\"\u003e\u003cstrong\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cimg data-mce-fragment=\"1\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"153\" height=\"43\" data-mce-selected=\"1\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\"\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"10 Preps","offer_id":44004217520228,"sku":"D331701","price":25500.0,"currency_code":"JPY","in_stock":true},{"title":"50 Preps","offer_id":44004217552996,"sku":"D331702","price":102700.0,"currency_code":"JPY","in_stock":true},{"title":"250 Preps","offer_id":44004246388836,"sku":"D331703","price":257700.0,"currency_code":"JPY","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/Screenshot_33.png?v=1674661475","url":"https:\/\/www.genebiosystems.com\/en-jp\/products\/solpure-hw-dna-kit","provider":"GeneBio ","version":"1.0","type":"link"}