{"product_id":"recombinant-rat-vegf-164","title":"Recombinant Rat VEGF 164","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:10ug\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDescription\u003c\/b\u003e:Recombinant Rat Vascular Endothelial Growth Factor A is produced by our Yeast expression system and the target gene encoding Ala27-Arg190(Ala36Thr) is expressed.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e:P16612-2\u003c\/p\u003e\u003cp\u003e\u003cb\u003eMolecular weight\u003c\/b\u003e:19.2 KDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApparent molecular weight\u003c\/b\u003e:18-23 KDa, reducing conditions\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOther names\u003c\/b\u003e:Vascular endothelial growth factor A; Vascular permeability factor; VEGF; VEGF-A; VPF\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage condition\u003c\/b\u003e:Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:Greater than 95% as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:Less than 0.1 ng\/µg (1 EU\/µg) as determined by LAL test.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBiological activity\u003c\/b\u003e:Immobilized  Rat VEGF 164(Cat#CJ96) at 2μg\/ml (100 μl\/well) can bind  Mouse VEGFR2-Fc(Cat#C01A).The ED50 of Mouse VEGFR2-Fc(Cat#C01A) is 22.17 ng\/ml.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRedissolve\u003c\/b\u003e:Always centrifuge tubes before opening.Do not mix by vortex or pipetting.\nIt is not recommended to reconstitute to a concentration less than 100μg\/ml.\nDissolve the lyophilized protein in distilled water.\nPlease aliquot the reconstituted solution to minimize freeze-thaw cycles. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e:Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks.\nReconstituted protein solution can be stored at 4-7°C for 2-7 days.\nAliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDelivery condition\u003c\/b\u003e:The product is shipped at ambient temperature.\nUpon receipt, store it immediately at the temperature listed below.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e:Vascular endothelial growth factor (VEGF\/VEGF-A ) is originally known as vascular permeability factor (VPF). It belongs to the PDGF family with a cysteine-knot structure comprised of eight conserved cysteine residues, and reckoned as a potent mediator in the process of angiogenesis and vasculogenesis in either fetus or adult. VEGF is particularly expressed in supraoptic , paraventricular nuclei and the choroid plexus of the pituitary, and abundant in the corpus luteum of the ovary and in kidney glomeruli. The rat VEGF protein contains a putative 20 amino acids (aa) signal peptide, and alternative splicing of rat VEGF gene produces isoforms of 120, 144, 164 and 188 aa. Rat VEGF164 respectively displays 97% and 88% aa identity with that regions of mouse and human VEGF. VEGF can bind to the FLT1\/VEGFR1 and KDR\/VEGFR2 receptors, heparan sulfate and heparin, and play important roles in inducing endothelial cell proliferation, promoting cell migration, inhibiting apoptosis and inducing permeabilization of blood vessels.\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48647904133220,"sku":"EPT063","price":61700.0,"currency_code":"JPY","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_0871b6e6-f415-402b-b6d1-1c3a9890828e.jpg?v=1782704426","url":"https:\/\/www.genebiosystems.com\/en-jp\/products\/recombinant-rat-vegf-164","provider":"GeneBio ","version":"1.0","type":"link"}