{"product_id":"recombinant-rat-gck-protein-his-tag","title":"Recombinant Rat GCK protein (His tag)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:100μg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShipping\u003c\/b\u003e:This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExp date\u003c\/b\u003e:12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_II\u003c\/b\u003e:Recombinant Proteins\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_III\u003c\/b\u003e:Others\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e:GCK\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Synonym\u003c\/b\u003e:GCK;Glucokinase;Hexokinase 4;Hexokinase-4\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e:Signal Transduction;Cardiovascular;Cancer;Metabolism\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e:Rat\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExpression Host\u003c\/b\u003e:E.coli\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFusion tag\u003c\/b\u003e:N-His\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUNIProt ID\u003c\/b\u003e:P17712\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e:P17712\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e:Catalyzes the phosphorylation of hexose, such as D-glucose, D-fructose and D-mannose, to hexose 6-phosphate (D-glucose 6-phosphate, D-fructose 6-phosphate and D-mannose 6-phosphate, respectively) (PubMed:6477520, PubMed:12513690, PubMed:24187134).\nCompared to other hexokinases, has a weak affinity for D-glucose, and is effective only when glucose is abundant (PubMed:6477520).\nMainly expressed in pancreatic beta cells and the liver and constitutes a rate-limiting step in glucose metabolism in these tissues (By similarity).\nSince insulin secretion parallels glucose metabolism and the low glucose affinity of GCK ensures that it can change its enzymatic activity within the physiological range of glucose concentrations, GCK acts as a glucose sensor in the pancreatic beta cell (By similarity).\nIn pancreas, plays an important role in modulating insulin secretion (By similarity).\nIn liver, helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage (By similarity).\nRequired to provide D-glucose 6-phosphate for the synthesis of glycogen (By similarity).\nMediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed:6477520, PubMed:12513690).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eActivity\u003c\/b\u003e:Not validated for activity\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e:Met 1-Gly 147\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e: \u0026gt; 95% as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e:Lyophilized from sterile PBS, pH 7.4.\u003cbr\u003eNormally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.\u003cbr\u003ePlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg\/mL. Concentration is measured by UV-Vis.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:\u0026lt; 10 EU\/mg of the protein as determined by the LAL method.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated MW\u003c\/b\u003e:16.1 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObservedMW\u003c\/b\u003e:16 kDa\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48644150198372,"sku":"PDER100176","price":154400.0,"currency_code":"JPY","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_e5242eff-ad7f-4bbe-b7ab-26dbcb477fc1.jpg?v=1782615503","url":"https:\/\/www.genebiosystems.com\/en-jp\/products\/recombinant-rat-gck-protein-his-tag","provider":"GeneBio ","version":"1.0","type":"link"}