{"product_id":"recombinant-human-dyrk3-redk-protein-his-gst-tag","title":"Recombinant Human DYRK3\/REDK Protein (His \u0026 GST Tag)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 50μg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShipping\u003c\/b\u003e: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExp date\u003c\/b\u003e: 12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_II\u003c\/b\u003e: Recombinant Proteins\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_III\u003c\/b\u003e: Others\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Synonym\u003c\/b\u003e: DYRK5;RED;REDK;hYAK3-2\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e: Epigenetics and Nuclear Signaling,Stem Cells,Cardiovascular\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e: Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExpression Host\u003c\/b\u003e: Baculovirus-Insect Cells\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: Enzyme\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFusion tag\u003c\/b\u003e: N-His-GST\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUNIProt ID\u003c\/b\u003e: O43781\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e: O43781-1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: Dual specificity  tyrosine-phosphorylation-regulated kinase 3, also known as Regulatory erythroid  kinase, REDK and DYRK3, is a nucleus protein which belongs to theprotein  kinase superfamily, CMGC Ser\/Thr protein kinase family and MNB\/DYRK subfamily.  DYRKs are an emerging family of dual-specificity kinases that play key roles in  cell proliferation, survival, and development. DYRK3 contains oneprotein  kinase domain. Isoform 1 and isoform 2 of DYRK3 are highly expressed in testis  and in hematopoietic tissue such as fetal liver, and bone marrow. Isoform 2 of  DYRK3 is the predominant form in testis. Isoform 1 of DYRK3 is the predominant  form in fetal liver and bone marrow. Isoform 1 and isoform 2 are present at low  levels in heart, pancreas, lymph node, and thymus. DYRK3 is a negative  regulator of EPO-dependent erythropoiesis. It may place an upper limit on red  cell production during stress erythropoiesis. DYRK3 inhibits cell death due to  cytokine withdrawal in hematopoietic progenitor cells. It may also act by  regulating CREB\/CRE signaling. DYRK3 proved to effectively inhibit NFAT  (nuclear factor of activated T cells) transcriptional response pathways and to  co-immunoprecipitate with NFATc3. DYRK3 attenuates (and possibly apportions)  red cell production selectively during anemia.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eActivity\u003c\/b\u003e: The specific activity was determined to be 22 nmol\/min\/mg using synthetic DYRKtide peptide (RRRFRPASPLRGPPK) as substrate. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e: Met 1-Ser 588\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:  \u0026gt; 85 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Supplied as sterile solution of 20mM Tris, 500mM NaCl, 0.5mM PMSF, 10% glycerol, pH 8.0\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e: Not Applicable\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:  \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated MW\u003c\/b\u003e: 93.5 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObservedMW\u003c\/b\u003e: 80 kDa\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":47972825399396,"sku":"PKSH030392","price":132800.0,"currency_code":"JPY","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_3e89ff44-ae35-4aed-821b-9ee06d7778eb.jpg?v=1772198103","url":"https:\/\/www.genebiosystems.com\/en-jp\/products\/recombinant-human-dyrk3-redk-protein-his-gst-tag","provider":"GeneBio ","version":"1.0","type":"link"}