{"product_id":"recombinant-human-c-yes-yes1-protein-his-gst-tag","title":"Recombinant Human c-Yes\/YES1 Protein (His \u0026 GST Tag)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 50μg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShipping\u003c\/b\u003e: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExp date\u003c\/b\u003e: 12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_II\u003c\/b\u003e: Recombinant Proteins\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_III\u003c\/b\u003e: Others\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Synonym\u003c\/b\u003e: HsT441;P61-YES;Yes;c-yes\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e: Signal Transduction;Neuroscience;Cancer;epigenetics and nuclear signal;\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e: Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExpression Host\u003c\/b\u003e: Baculovirus-Insect Cells\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: Enzyme\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFusion tag\u003c\/b\u003e: N-His-GST\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUNIProt ID\u003c\/b\u003e: P07947\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e: NP_005424.1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: Proto-oncogene tyrosine-protein  kinase Yes, also known as Proto-oncogene c-Yes, p61-Yes and YES1, is a  cytoplasm protein which belongs to theprotein kinase superfamily, Tyr  protein kinase family and SRC subfamily. YES1 \/ c-Yes contains oneprotein  kinase domain, oneSH2 domain and oneSH3 domain. It is thought that  the subcellular distribution of Src-family tyrosine kinases, including c-Yes  binding to the cellular membrane, is membranous and\/or cytoplasmic. YES1 \/  c-Yes protein tyrosine kinase is known to be related to malignant  transformation. YES1 \/ c-Yes and c-Src are the two most closely related members  of the Src family of nonreceptor tyrosine kinases. Although there is much  evidence to support redundancy in signaling between these two kinases. YES1 \/  c-Yes promotes formation of the tight junction by phosphorylating occludin,  while c-Src signaling downregulates occludin formation in a Raf-1 dependent  manner. YES1 \/ c-Yes has tyrosine kinase activity. It promotes infectivity of  Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP \/ CD46.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eActivity\u003c\/b\u003e: The specific activity was determined to be 35 nmol\/min\/mg using Poly(Glu, Tyr) 4:1 as substrate. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e: Gly 2-Leu 543\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:  \u0026gt; 80 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Supplied as sterile solution of 20mM Tris, 500mM NaCl, 10% glycerol, 0.5mM TCEP, pH 8.0\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e: Not Applicable\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:  \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated MW\u003c\/b\u003e: 88.5 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObservedMW\u003c\/b\u003e: 75 kDa\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":47972819173476,"sku":"PKSH030348","price":132400.0,"currency_code":"JPY","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_903446a1-d386-4d7e-b8dc-dd3b7f21f7c2.jpg?v=1772197839","url":"https:\/\/www.genebiosystems.com\/en-jp\/products\/recombinant-human-c-yes-yes1-protein-his-gst-tag","provider":"GeneBio ","version":"1.0","type":"link"}