{"product_id":"purified-mouse-anti-histone-h3-antibody","title":"Purified Mouse anti-Histone H3 Antibody","description":"\u003cp\u003e\u003cb\u003eSize 1\u003c\/b\u003e: 100 ul\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSynonyms \u003c\/b\u003e: HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3\/a; Histone H3\/b; Histone H3\/c; Histone H3\/d; Histone H3\/f; Histone H3\/h; Histone H3\/i; Histone H3\/j; Histone H3\/k; Histone H3\/l; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3D; Histone H3.2; Histone H3\/m; Histone H3\/o; H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3\u003c\/p\u003e\u003cp\u003e\u003cb\u003ehost\u003c\/b\u003e: Mouse\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）\u003c\/b\u003e: H, M, R, Yeast\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplications\u003c\/b\u003e: WB, IF\/IC, IP\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: Mouse monoclonal antibody to Histone H3\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: 1mg\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eImmunogen\u003c\/b\u003e: Recombinant protein corresponding to human Histone H3.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWB description\u003c\/b\u003e: Western blot analysis of Histone H3 expression in Hela (A), Raw264.7 (B), mouse brain (C), rat brain (D) whole cell lysates.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIHC description\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eIF\/ICC description\u003c\/b\u003e: Immunofluorescent analysis of Histone H3 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIP description\u003c\/b\u003e: Immunoprecipitation of Histone H3 from 0.5mg Hela whole cell extract lysate, using  anti-Histone H3 Antibody.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFC description\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eChIP description\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurification_Method\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality\u003c\/b\u003e: Monoclonal\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eBuffer\u003c\/b\u003e: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDilution\u003c\/b\u003e: WB (1\/2000 - 1\/5000), IF\/IC (1\/100 - 1\/500), IP (1\/50 - 1\/200)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene ID(Human)\u003c\/b\u003e: 8350; 8351; 8352; 8353; 8354; 8355; 8356; 8357; 8358; 8968\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene ID (Mouse)\u003c\/b\u003e: 319152; 15077; 15078\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene ID(Rat)\u003c\/b\u003e: 291159; 100361558\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSwissProt (Human)\u003c\/b\u003e: P68431; Q71DI3; P84243\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSwissProt (Mouse)\u003c\/b\u003e: P68433; P84228; P84244\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSwissProt (Rat)\u003c\/b\u003e: Q6LED0; P84245\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Store at -20℃. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48738515058788,"sku":"DL95527A","price":123300.0,"currency_code":"JPY","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_c6971e1c-a8f8-481d-8775-3724cce05a42.jpg?v=1783811265","url":"https:\/\/www.genebiosystems.com\/en-jp\/products\/purified-mouse-anti-histone-h3-antibody","provider":"GeneBio ","version":"1.0","type":"link"}