{"product_id":"human-ck-18-m65-cytokeratin-18-m65-elisa-kit","title":"Human CK 18-M65 (Cytokeratin 18-M65) ELISA Kit","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:96T\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e:Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAlternative Names\u003c\/b\u003e:K18-M65; CK-18-M65; CYK18-M65\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay Type\u003c\/b\u003e:Sandwich\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSensitivity\u003c\/b\u003e:29.8 pg\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStandard\u003c\/b\u003e:5000 pg\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDetection range\u003c\/b\u003e:78.13-5000 pg\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSample type\u003c\/b\u003e:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay length\u003c\/b\u003e:3.5h\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Area\u003c\/b\u003e:Cancer of the liver; Viral hepatitis\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTest principle\u003c\/b\u003e:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CK 18-M65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CK 18-M65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CK 18-M65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CK 18-M65 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniProt ID\u003c\/b\u003e:\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48644911300708,"sku":"ELK10045","price":120800.0,"currency_code":"JPY","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_7943ac33-73ba-44a3-b6f7-b01c07f2846d.jpg?v=1782660619","url":"https:\/\/www.genebiosystems.com\/en-jp\/products\/human-ck-18-m65-cytokeratin-18-m65-elisa-kit","provider":"GeneBio ","version":"1.0","type":"link"}