{"product_id":"human-alpha-fetoprotein-lens-culinaris-agglutiin-3-afp-l3-elisa-kit","title":"Human Alpha-fetoprotein Lens culinaris agglutiin 3 (AFP-L3) ELISA Kit","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 96T. Other sizes are also available. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecies Reactivity\u003c\/b\u003e: Human (Homo sapiens)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniProt\u003c\/b\u003e: N\/A\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e: AFP-L3\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAlternative Names\u003c\/b\u003e: N\/A\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: ELISA\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRange\u003c\/b\u003e: 0.625-40 ng\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSensitivity\u003c\/b\u003e: 0.244 ng\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTest principle\u003c\/b\u003e: This assay employs a two-site sandwich ELISA to quantitate AFP-L3 in samples. An antibody specific for AFP-L3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAFP-L3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AFP-L3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AFP-L3 bound in the initial step. The color development is stopped and the intensity of the color is measured.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Overview\u003c\/b\u003e: AFP-L3 is one of AFP fractions which is synthesized by malignant cell and comprise special oligosaccharide.It is a new generation of tumor marker for HCC and yields useful information on HCC for clinical decision making. Reports at home or abroad have showed that if the ratio of AFP-L3 increased over 10%, the risk of catching HCC had increased highly, and non-malignant diseases hardly synthesize this AFP fraction(AFP-L3).AFP-L3 can be detected 9-12 months earlier and shows the remarkable precaution effect to HCC. AFP-L3 is the fucosylated variant of AFP produced specifically by HCC. AFP-L3 bears strong affinity to the sugar chain of Lens culinaris, a type of lentil. AFP-L3 is less sensitive but more specific than AFP, and therefore cannot replace AFP. The value of AFP-L3 lies mainly in distinguishing HCC from other conditions when AFP is elevated.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStability\u003c\/b\u003e: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. \nThe loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":39778297643108,"sku":"AE24203HU","price":148300.0,"currency_code":"JPY","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/no_image_default_image-jpeg_1d42dcae-c8d4-4aca-90cd-587b51b919ea.jpg?v=1660518544","url":"https:\/\/www.genebiosystems.com\/en-jp\/products\/human-alpha-fetoprotein-lens-culinaris-agglutiin-3-afp-l3-elisa-kit","provider":"GeneBio ","version":"1.0","type":"link"}