{"product_id":"fs-fast-dna-polymerase-2x-master-mix","title":"FS Fast DNA Polymerase 2X Master Mix","description":"\u003cp\u003e\u003cspan\u003eThe same high-performance Taq DNA polymerase, configured in a Master Mix format for your convenience. Fast Taq DNA polymerase is the latest generation Taq-based DNA polymerase. It possesses high amplification efficiency as does Taq polymerase, and fast elongation ability as does KOD polymerase, can be used in a variety of PCR. The FS\u003csup\u003eTM\u003c\/sup\u003e PCR Buffer, designed for FS\u003csup\u003eTM\u003c\/sup\u003e Taq DNA polymerase, can be used in fast amplification reaction. The elongation rate of FS\u003csup\u003eTM\u003c\/sup\u003e Taq DNA polymerase is more than twice the rate by regular Taq DNA polymerase, which shortens the amplification time at least by half , as fast as \u003cspan\u003e5s\/kb for simple sequence templates\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e Features\u003cbr\u003e Fast rate of elongation: elongation rate can reach to 3 kb\/min, more than twice the rate by regular Taq DNA polymerase;\u003cbr\u003e Highly thermostable : have a half-life of over 40 min at 95\u003cspan\u003e°\u003c\/span\u003eC incubation Generates 3'-dA overhangs PCR products.\u003cbr\u003e\u003cbr\u003e Applications\u003cbr\u003e Routine PCR\u003cbr\u003e PCR labeling\u003cbr\u003e PCR sequencing\u003cbr\u003e Generate PCR product for TA cloning\u003cbr\u003e\u003cbr\u003e Unit Definition\u003cbr\u003e One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70\u003cspan\u003e°\u003c\/span\u003eC using hering sperm DNA as substrate.\u003cbr\u003e\u003cbr\u003e Quality Control \u003cbr\u003e The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases is confirmed by appropriate quality tests\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFunctionally tested in amplification of a single-copy gene from human genomic DNA\u003cbr\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStore all components at \u003cspan\u003e–\u003c\/span\u003e20\u003cspan\u003e°\u003c\/span\u003eC\u003cbr\u003e\u003cbr\u003e Protocol for PCR\u003cbr\u003e All solutions should be thawed on ice, gently vortexed and briefly centrifuged.\u003cbr\u003e Add in a thin walled PCR tube on ice:\u003c\/span\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"1 ml","offer_id":39766543892580,"sku":"P2070","price":5600.0,"currency_code":"JPY","in_stock":true},{"title":"5 ml","offer_id":39766543925348,"sku":"P2070","price":20900.0,"currency_code":"JPY","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/242.jpg?v=1643677274","url":"https:\/\/www.genebiosystems.com\/en-jp\/products\/fs-fast-dna-polymerase-2x-master-mix","provider":"GeneBio ","version":"1.0","type":"link"}