{"product_id":"anti-nfkb-nfkb2-p100-p52-antibody-picoband-r","title":"Anti-NFkB\/NFKB2 p100\/p52 Antibody Picoband®","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 100 μg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eForm\u003c\/b\u003e: Lyophilized\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e: Rat,Mouse\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplications\u003c\/b\u003e: IHC,WB\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication Details\u003c\/b\u003e: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg\/ml, Rat, Mouse\u003cbr\u003eWestern blot, 0.1-0.5μg\/ml, Mouse, Rat\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene Name\u003c\/b\u003e: NFKB2\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: No cross reactivity with other proteins.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: NFKB2 (Nuclear Factor Kappa-B, Subunit 2), also known as NFKB or p52\/p100 SUBUNIT, is a protein that in humans is encoded by the NFKB2 gene. Liptay et al. (1992) mapped the gene for what they called the p49\/p100 subunit of NFKB (NFKB2) to chromosome 10 by Southern blot analysis of panels of human\/Chinese hamster cell hybrids. By fluorescence in situ hybridization (FISH), they confirmed the localization and mapped the gene with greater resolution to 10q24. NFKB2 appears to be the same as LYT10. Claudio et al. (2002) showed that bone marrow (BM) cells from Nfkb2-deficient mice, but not Nfkb1-deficient mice, failed to increase relative and total IgD-positive transitional-1 (T1) stage B cells in response to Baff. In vivo, however, Nfkb2-deficient mice did generate mature B cells, but at reduced numbers. Mice of the aly\/aly strain, which are naturally deficient in Nik, and mice of the A\/WySNJ strain, which have a mutation in Baffr, also failed to produce T1 B cells in response to Baff. Baff stimulation enhanced expression of Bcl2 in T1 B cells, thereby promoting B-cell survival, and caused the processing of the p100 form of Nfkb2 to p52, which again required Baffr and Nik, but not Nemo (IKKG). Immunoblot analysis showed that BM cells contained primarily p100.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eImmunogen\u003c\/b\u003e: A synthetic peptide corresponding to a sequence at the N-terminus of mouse NFkB\/NFKB2 p100\/p52, identical to the related rat sequence.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality\u003c\/b\u003e: Polyclonal\u003c\/p\u003e\u003cp\u003e\u003cb\u003eContents\u003c\/b\u003e: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.\u003cbr\u003e\n*This antibody is supplied in a stabilized formulation. \nCompatibility with conjugation reactions depends on the chemistry of the conjugation method used. \nFor conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurification\u003c\/b\u003e: Immunogen affinity purified.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e: Add 0.2ml of distilled water will yield a concentration of 500ug\/ml.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReference\u003c\/b\u003e: 1. Claudio, E., Brown, K., Park, S., Wang, H., Siebenlist, U. BAFF-induced NEMO-independent processing of NF-kappa-B2 in maturing B cells. Nature Immun. 3: 958-965, 2002.\n2. Liptay, S., Schmid, R. M., Perkins, N. D., Meltzer, P., Altherr, M. R., McPherson, J. D., Wasmuth, J. J., Nabel, G. J. Related subunits of NF-kappa-B map to two distinct loci associated with translocations in leukemia, NFKB1 and NFKB2. Genomics 13: 287-292, 1992.\n3. Tucker, E., O'Donnell, K., Fuchsberger, M., Hilton, A. A., Metcalf, D., Greig, K., Sims, N. A., Quinn, J. M., Alexander, W. S., Hilton, D. J., Kile, B. T., Tarlinton, D. M., Starr, R. A novel mutation in the Nfkb2 gene generates an NF-kappa-B2 'super repressor' J. Immun. 179: 7514-7522, 2007.\n\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniprot ID\u003c\/b\u003e: Q9WTK5\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHost\u003c\/b\u003e: Rabbit\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: Adding 0.2 ml of distilled water will yield a concentration of 500 μg\/ml.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugate\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eCross Reactivity\u003c\/b\u003e: No cross-reactivity with other proteins\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e: Rabbit IgG\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePhospho_site\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone Number\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eObserved Molecular Weight\u003c\/b\u003e: 43 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated Molecular Weight\u003c\/b\u003e: 96832 MW\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene ID\u003c\/b\u003e: 18034\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProtein Name\u003c\/b\u003e: Nuclear factor NF-kappa-B p100 subunit\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene Full Name\u003c\/b\u003e: nuclear factor of kappa light polypeptide gene enhancer in B cells 2, p49\/p100\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSynonyms\u003c\/b\u003e: DNA binding factor KBF2; H2TF1; LYT 10; LYT10; NF kappa B; NFKB2; NFKB2; p100; Oncogene Lyt 10; p100\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48787304349796,"sku":"PA1946","price":94200.0,"currency_code":"JPY","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_975d1b31-fa39-4d03-97bd-1b075743910f.jpg?v=1784398421","url":"https:\/\/www.genebiosystems.com\/en-jp\/products\/anti-nfkb-nfkb2-p100-p52-antibody-picoband-r","provider":"GeneBio ","version":"1.0","type":"link"}