{"product_id":"adpn-elisa-kit-for-rat","title":"ADPN ELISA kit (Rat)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 96Tests\u003c\/p\u003e\u003cp\u003e\u003cb\u003e# of Times Cited in literature\u003c\/b\u003e: 30\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePrepare Time\u003c\/b\u003e: 1-3 days(please inquire for mutiple units)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Name\u003c\/b\u003e: ADPN\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Full Name\u003c\/b\u003e: Adiponectin\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAlternative Names\u003c\/b\u003e: GBP28; ApM1; AdipoQ; Acrp30; ACDC; APM1; C1Q And Collagen Domain Containing; Adipocyte Complement-Related Protein Of 30 KDa; Adipose Most Abundant Gene Transcript 1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e: Rat\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniprot\u003c\/b\u003e: Q8K3R4\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene ID\u003c\/b\u003e: 246253\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFeatured Series\u003c\/b\u003e: SE kit\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFeatured Series Function\u003c\/b\u003e: Detects protein (regular version)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: Reactive with Rat ADPN \/ Adiponectin\u003c\/p\u003e\u003cp\u003e\u003cb\u003eMethod\u003c\/b\u003e: Colormetric\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDetection principle\u003c\/b\u003e: Double-antibody Sandwich\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDetection\nrange\u003c\/b\u003e: 0.312-20ng\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSensitivity\u003c\/b\u003e: 0.127ng\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay Time\u003c\/b\u003e: 3h\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSample Size\u003c\/b\u003e: 100uL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRecommended\/Predicted\nSample Types\u003c\/b\u003e: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay Precision\u003c\/b\u003e: Intra-Assay: CV\u0026lt;10%, Inter-Assay: CV\u0026lt;12%\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReproducibility test menthod\u003c\/b\u003e: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adiponectin (ADPN) were tested 20 times on one plate, respectively.\nInter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adiponectin (ADPN) were tested on 3 different plates, 8 replicates in each plate.\nCV(%) = SD\/meanX100\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: 4°C for 1 month\/ -20°C for long-term(One year within shelf life)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShelf-life\u003c\/b\u003e: 12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: This assay has high sensitivity and excellent specificity for detection of Adiponectin (ADPN).\nNo significant cross-reactivity or interference between Adiponectin (ADPN) and analogues was observed.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStability\u003c\/b\u003e: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.\nTo minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay procedure summary\u003c\/b\u003e: 1. Prepare all reagents, samples and standards;\n2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;\n3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;\n4. Aspirate and wash 3 times;\n5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;\n6. Aspirate and wash 5 times;\n7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;\n8. Add 50µL Stop Solution. Read at 450nm immediately.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTest principle\u003c\/b\u003e: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adiponectin (ADPN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adiponectin (ADPN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adiponectin (ADPN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adiponectin (ADPN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Area\u003c\/b\u003e: Metabolic pathway;Infection immunity;Cardiovascular biology;\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReferences Citing This Product\u003c\/b\u003e: \u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/20869961\"\u003ePuerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat diet\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3591142\/\"\u003eSerum Fetuin-A Levels Related with Microalbuminuria in Diet-Induced Obese Rats\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/24526332\"\u003ePerirenal fat associated with microalbuminuria in obese rats\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC4046234\/\"\u003eZataria multiflora increases insulin sensitivity and PPARγ gene expression in high fructose fed insulin resistant rats\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/24519243\"\u003ePerinatal low-dose iron treatment influences susceptibility to diet-induced adipogenesis in early-aged male Wistar rats\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25194956\"\u003eAdipose tissue chromium and vanadium disbalance in high-fat fed Wistar rats\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25240061\"\u003eCentral alarin ameliorated insulin resistance of adipocytes in type 2 diabetic rats\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25697126\"\u003eLong-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26160485%20\"\u003eInduction of Haemeoxygenase-1 Directly Improves Endothelial Function in Isolated Aortas from Obese Rats through the Ampk-Pi3k\/Akt-Enos Pathway\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26896925\"\u003eSwimming training attenuates the morphological reorganization of the myocardium and local inflammation in the left ventricle of growing rats with untreated experimental diabetes.\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.food.actapol.net\/volume15\/issue1\/abstract-10.html\"\u003eDecreased adipose tissue zinc content is associated with metabolic parameters in high fat fed Wistar rats\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S0065128116301234\"\u003eFavorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC4817393\/\"\u003eTrigonella foenum-graecum water extract improves insulin sensitivity and stimulates PPAR and γ gene expression in high fructose-fed insulin-resistant rats\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/link.springer.com\/article\/10.1007\/s12011-016-0777-1\"\u003eEarly High-Fat Feeding Induces Alteration of Trace Element Content in Tissues of Juvenile Male Wistar Rats\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26175082\"\u003eFat and Sucrose Intake Induces Obesity‐Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult Rats\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27127795\"\u003eCentral administration of galanin receptor 1 agonist boosted insulin sensitivity in adipose\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29147484\"\u003eBerberis integerrima ameliorates insulin resistance in high-fructose-fed insulin-resistant rats\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.hindawi.com\/journals\/omcl\/2017\/3405089\/abs\/\"\u003eDifferential Susceptibility of Germ and Leydig Cells to Cadmium-Mediated Toxicity: Impact on Testis Structure, Adiponectin Levels, and Steroidogenesis\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29201096\"\u003eAntihyperglycemic Effect of Rosa Damascena is Mediated by PPAR.γ Gene Expression in Animal Model of Insulin Resistance\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.ijge.2018.01.002\"\u003eAging effects on circulating adiponectin levels and the expressions of adiponectin and adiponectin receptor 1 in the brains of male rats\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/jbrms.medilam.ac.ir\/article-1-352-en.pdf\"\u003eDecreased level of the anti-inflammatory adipokines, secreted frizzled-related protein 5 and adiponectin, in high cholesterol diet-induced atherosclerotic rats\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S1347861318301828\"\u003eImproved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndrome\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S002432051930147X\"\u003eInterrelation of liver vascularity to non-alcoholic fatty liver through a comparative study of the vasodilator effect of carvedilol or nicorandil in rats\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32970613\/\"\u003eResemblance and differences in dietary restriction nephroprotective mechanisms in young and old rats\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32588381\/\"\u003eActivation of central galanin receptor 2 mitigated insulin resistance in adipocytes of diabetic rats\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32395890\/\"\u003eCooperative effects of galanin and leptin on alleviation of insulin resistance in adipose tissue of diabetic rats\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/34035808\"\u003eEffect of Ileal Transposition (IT) on Angiopoietin-Like Protein-8 (ANGPTL8) and Pentraxin (PTX3) Plasma Level in Sprague-Dawley Rats Fed High-Fat Diet?¡­\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48696701681764,"sku":"SEA605Ra","price":179900.0,"currency_code":"JPY","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_9592ce76-d87c-4069-8e67-3453e78e598b.jpg?v=1783134637","url":"https:\/\/www.genebiosystems.com\/en-jp\/products\/adpn-elisa-kit-for-rat","provider":"GeneBio ","version":"1.0","type":"link"}