{"product_id":"8-ohdg-elisa-kit-for-general-species","title":"8-OHdG ELISA kit (General species)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 96Tests\u003c\/p\u003e\u003cp\u003e\u003cb\u003e# of Times Cited in literature\u003c\/b\u003e: 74\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePrepare Time\u003c\/b\u003e: 1-3 days(please inquire for mutiple units)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Name\u003c\/b\u003e: 8-OHdG\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Full Name\u003c\/b\u003e: 8-Hydroxydeoxyguanosine\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAlternative Names\u003c\/b\u003e: 8OHdG; 7,8-Dihydro-8-Oxo-2'-Deoxyguanosine; 7,8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2'-Deoxyguanosine; 8-Oxo-DG\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e: General species\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniprot\u003c\/b\u003e: -\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene ID\u003c\/b\u003e: -\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFeatured Series\u003c\/b\u003e: CE kit\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFeatured Series Function\u003c\/b\u003e: Detects small molecule\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: Reactive with General species 8-OHdG \/ 8-Hydroxydeoxyguanosine\u003c\/p\u003e\u003cp\u003e\u003cb\u003eMethod\u003c\/b\u003e: Colormetric\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDetection principle\u003c\/b\u003e: Competitive Inhibition\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDetection\nrange\u003c\/b\u003e: 74.07-6,000pg\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSensitivity\u003c\/b\u003e: 26.81pg\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay Time\u003c\/b\u003e: 2h\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSample Size\u003c\/b\u003e: 50uL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRecommended\/Predicted\nSample Types\u003c\/b\u003e: Serum, Plasma and other Biological Fluids\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay Precision\u003c\/b\u003e: Intra-Assay: CV\u0026lt;10%, Inter-Assay: CV\u0026lt;12%\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReproducibility test menthod\u003c\/b\u003e: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 8-Hydroxydeoxyguanosine (8-OHdG) were tested 20 times on one plate, respectively.\nInter-assay Precision (Precision between assays): 3 samples with low, middle and high level 8-Hydroxydeoxyguanosine (8-OHdG) were tested on 3 different plates, 8 replicates in each plate.\nCV(%) = SD\/meanX100\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: 4°C for 1 month\/ -20°C for long-term(One year within shelf life)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShelf-life\u003c\/b\u003e: 12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: This assay has high sensitivity and excellent specificity for detection of 8-Hydroxydeoxyguanosine (8-OHdG).\nNo significant cross-reactivity or interference between 8-Hydroxydeoxyguanosine (8-OHdG) and analogues was observed.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStability\u003c\/b\u003e: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.\nTo minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay procedure summary\u003c\/b\u003e: 1. Prepare all reagents, samples and standards;\n2. Add 50µL standard or sample to each well.\n    And then add 50µL prepared Detection Reagent A immediately.\n    Shake and mix. Incubate 1 hour at 37°C;\n3. Aspirate and wash 3 times;\n4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;\n5. Aspirate and wash 5 times;\n6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;\n7. Add 50µL Stop Solution. Read at 450 nm immediately.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTest principle\u003c\/b\u003e: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 8-Hydroxydeoxyguanosine (8-OHdG) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 8-Hydroxydeoxyguanosine (8-OHdG) and unlabeled 8-Hydroxydeoxyguanosine (8-OHdG) (Standards or samples) with the pre-coated antibody specific to 8-Hydroxydeoxyguanosine (8-OHdG). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 8-Hydroxydeoxyguanosine (8-OHdG) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 8-Hydroxydeoxyguanosine (8-OHdG) in the sample.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Area\u003c\/b\u003e: Metabolic pathway;Tumor immunity;Infection immunity;Endocrinology;Hematology;Hepatology;\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReferences Citing This Product\u003c\/b\u003e: \u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/24142649\"\u003eIodinated contrast media can induce long-lasting oxidative stress in hemodialysis patients.\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26385569\"\u003eGlycyrrhizic Acid Prevents Sepsis-Induced Acute Lung Injury and Mortality in Rats\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dmrr.2814\/full\"\u003eReduced peripheral blood mtDNA content is associated with impaired glucose‐stimulated islet β cell function in a Chinese population with different degrees of …\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27071648\"\u003eInfluence of diet on leukocyte telomere length, markers of inflammation and oxidative stress in individuals with varied glucose tolerance: a Chinese population study\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC4855822\/\"\u003eOxidative DNA damage and oxidized low density lipoprotein in Type II diabetes mellitus among patients with Helicobacter pylori infection\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/uvadoc.uva.es\/bitstream\/10324\/20828\/1\/Tesis1149-161108.pdf\"\u003eDieta hipercalórica e hipoxia intermitente crónica como modelos de obesidad y apnea obstructiva del sueño. Significado de la leptina en el control respiratorio\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.researchgate.net\/profile\/Wesam_Nasef3\/publication\/311081650_JDM_2016112911313922\/links\/583d521a08aeb3987e3100ef.pdf\"\u003eThe Activity of Serum 8-Iso-Prostaglandin F2α as Oxidative Stress Marker in Patients with Diabetes Mellitus Type 2 and Associated Dyslipidemic Hyperglycemia\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/link.springer.com\/article\/10.1007\/s12288-016-0718-3\"\u003eOleuropein Ameliorates Cisplatin-induced Hematological Damages Via Restraining Oxidative Stress and DNA Injury\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27478848\"\u003eOxidative Damage to the Salivary Glands of Rats with Streptozotocin-Induced Diabetes-TemporalStudy: Oxidative Stress and Diabetic Salivary Glands.\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28184199\"\u003eOxidative Modification in the Salivary Glands of High Fat-Diet Induced Insulin Resistant Rats.\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28926744\"\u003eChronic high-protein diet induces oxidative stress and alters the salivary gland function in rats\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/doi.org\/10.1155\/2017\/4923769\"\u003eOxidative modification of biomolecules in the non-stimulated and stimulated saliva of patients with morbid obesity treated with bariatric surgery\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29053628\"\u003eAntioxidant Defence, Oxidative Stress and Oxidative Damage in Saliva, Plasma and Erythrocytes of Dementia Patients. Can Salivary AGE be a Marker of Dementia?\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/doi.org\/10.1089\/ars.2016.6758\"\u003eChemokine Receptor CXCR4 Plays a Crucial Role in Mediating Oxidative StressInduced Podocyte Injury\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ijcem.com\/files\/ijcem0048822.pdf\"\u003eMelatonin ameliorates oxidative DNA damage and protects against formaldehyde-induced oxidative stress in rats\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28616770\"\u003eProtective Effect of Origanum Oil on Alterations of Some Trace Elements and Antioxidant Levels Induced by Mercuric Chloride in Male Rats\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/emmind.net\/openpapers_repos\/Applied_Fields-Hazads\/Microwave_Effects\/Phone_2G-3G\/2017_Micronuclei_Formation_and_8-Hydroxy-2-Deoxyguanosine_Enzyme_Detection_in_Ovarian_Tissues_After_Radiofrequency_Exposure_at_1800_M.pdf\"\u003eMicronuclei Formation and 8-Hydroxy-2-Deoxyguanosine Enzyme\u003c\/a\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48696686444644,"sku":"CEA660Ge","price":209300.0,"currency_code":"JPY","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_ec318ab0-3540-40cf-993b-dabb0cf7bd2b.jpg?v=1783134115","url":"https:\/\/www.genebiosystems.com\/en-jp\/products\/8-ohdg-elisa-kit-for-general-species","provider":"GeneBio ","version":"1.0","type":"link"}