{"title":"PCR Reagents","description":"\u003cp\u003eA polymerase chain reaction (PCR) is a crucial technique used to rapidly produce copies of a specific part of a DNA in masses-usually trillions of copies. Reagents in this context are the biochemical components, such as polymerases, dNTP, magnesium, minerals and buffers that PCR requires to duplicate a genetic material successfully.\u003c\/p\u003e\n\u003cp\u003e· The primary ingredients necessary for a complete PCR reaction include:\u003c\/p\u003e\n\u003cp\u003e- DNA template: this is a sample DNA containing the target sequence\u003c\/p\u003e\n\u003cp\u003e- DNA polymerase: a unique enzyme that synthesizes new DNA strands to complement the target sequence\u003c\/p\u003e\n\u003cp\u003e- Primers: short pieces of single-stranded DNA which complement the target sequence\u003c\/p\u003e\n\u003cp\u003e- Nucleotides: building blocks for new DNA strands\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e· Gene Bio Systems emphasizes that the success of the PCR technique is part of many molecular biological applications. Our range of products supports various processes, including PCR amplification for difficult to amplification templates, for applications in which the fidelity of amplification is important, for applications in which the DNA template may not have been fully purified. We have the PCR reagents for most of the common molecular applications.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e· Like other scientific processes, it is worth noting that PCR reactions exhibit a lot of complexity. The success of various PCR amplification reactions depends on the quality and performance of appropriate PCR enzymes, master mixes and ancillary reagents. The the reagents, or anything less than robust and rational design in the reagents set back the entire PCR process; thus, getting products from a reliable source is crucial. Gene Bio Systems delivers exactly that. You can buy our products online and reach us for more support.\u003c\/p\u003e\n\u003cp\u003e· PCR reagents include reagents and kits for real-time PCR, end-point, hot-start, rapid PCR, long range PCR, and robust PCR for difficult to amplify template\/target DNA. Reagents also include PCR product purification, and many other PCR related products.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eMouse Genotyping Kit\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/Mouse_Genotyping_Kit_Brochure.pdf?v=1684867408\"\u003e Brochure\u003c\/a\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eQuickEasy Cell Direct \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/QuickEasy_Cell_Direct_RT-qPCR_Kit_SYBR_Green_I.pdf?v=1684867780\"\u003eBrochure\u003c\/a\u003e\u003c\/span\u003e\u003c\/p\u003e","products":[{"product_id":"gb-amp-2-x-pcr-master-mix-with-loading-dye","title":"GB-Amp™ 2X PCR Master mix (with loading dye)","description":"\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003ePCR Master Mix is a premixed solution containing all essential components and reagents required to conduct a PCR assay. It significantly simplifies the analysis of PCR products and the preparation of PCR samples. It is specified for\u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e \u003c\/span\u003e\u003cspan style=\"font-weight: 400;\"\u003erequiring high sensitivity, such as somatic mutations and microbial DNA detection. Generally, the master mix catalog includes thermostable Taq DNA polymerase recombinant, dNTPs, stabilizers, cofactor (MgCl2), and enhancers, all in a reaction buffer.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cb\u003eApplications of GB-Amp™ 2X PCR Master Mix Products\u003c\/b\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli style=\"font-weight: 400;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003eRoutine PCR\u003c\/span\u003e\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003ePrimer dimers reduction\u003c\/span\u003e\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003eVarious primer-extension reactions, including labeling, signaling, and sequencing\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\n\u003cb\u003e\u003c\/b\u003e\u003cbr\u003e\n\u003c\/h2\u003e\n\u003ch2\u003e\u003cb\u003eFeatures and Benefits of our Product\u003c\/b\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli style=\"font-weight: 400;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003eIncredible amplification — The GB-Amp™ 2X PCR Master mix guarantees up to 6 kb amplification of PCR products.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003eIt is resistant to impurities.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003e\n\u003cspan style=\"font-weight: 400;\"\u003eQuick and convenient — Our 2x master mixes are equipped with gel loading dyes and other additives, including Taq DNA polymers facilitating direct loading of the products and templates onto the agarose gels.\u003c\/span\u003e \u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eThe GB-Amp™ 2X PCR Master mix is designed to make work easier, save time, and eliminate errors in proportioning and measurements. If you have been looking to streamline and improve your lab experiments, the state-of-the-art GB-Amp™ 2X PCR Master mix is a great option. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eDo you need to use both Taq, Pfu or any other proofreading polymerase for PCR? In this case, our 5min TA-Blunt-Zero Cloning Kit is the perfect choice. This is where we come in. We have your back, no matter the scope and size of your laboratory. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/GB-Amp_2_x_PCR_Master_mix_with_loading_dye.pdf?v=1700752097\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e\u003cspan\u003e\u003cimg height=\"46\" width=\"164\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"1mL","offer_id":39752493170788,"sku":"P3001","price":22.83,"currency_code":"CAD","in_stock":true},{"title":"5mL","offer_id":39752493203556,"sku":"P3002","price":98.09,"currency_code":"CAD","in_stock":true},{"title":"10mL","offer_id":39752493236324,"sku":"P3003","price":166.96,"currency_code":"CAD","in_stock":true},{"title":"50mL","offer_id":39752493269092,"sku":"P3004","price":728.95,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/2_2e0d719f-3c21-459e-be9c-3155512f5fe6.jpg?v=1751570904"},{"product_id":"pfu-dna-polymerase","title":"Pfu DNA Polymerase","description":"\u003cp\u003ePfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has been shown to exhibit superior thermostability and proofreading properties compared to other thermostable polymerase. Unlike Taq DNA polymerase, highly thermostable Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA polymerase in your PCR reactions results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis.\u003c\/p\u003e\n\u003cp\u003eComponents: Pfu DNA polymerase (2.5 U\/ul or 5\u003cspan data-mce-fragment=\"1\"\u003e U\/ul-please see user manual\u003c\/span\u003e), 10x Pfu buffer\u003cbr\u003e\u003cbr\u003eFeatures\u003cbr\u003e3'-5' exonuclease activity provides a low error rate\u003cbr\u003eOne of the most thermostable DNA polymerases known\u003cbr\u003eLack of extendase activity means no unwanted 3’ overhangs\u003cbr\u003eOptimal for blunt-ended PCR cloning\u003cbr\u003eOptimum temperature near 75°C\u003cbr\u003e95% active after 1hour incubation at 98°C\u003cbr\u003e\u003cbr\u003eApplications\u003cbr\u003eHigh-fidelity PCR and primer-extension reactions\u003cbr\u003ePCR cloning and blunt-ended amplification product generation\u003cbr\u003e\u003cbr\u003eUnit Definition\u003cbr\u003eOne unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.\u003cbr\u003e\u003cbr\u003eStore all components at –20°C\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/B600003_EN_P-UM-Pfu_DNA_Polymerase-GBS.pdf?v=1700584401\"\u003e\u003cimg height=\"42\" width=\"149\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\"\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"250 U","offer_id":39752493957220,"sku":"P1021","price":52.67,"currency_code":"CAD","in_stock":true},{"title":"1000 U","offer_id":39752493989988,"sku":"P1023","price":164.61,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_f893e40b-0727-4daa-a0e2-7769aa7f6e68.jpg?v=1706709482"},{"product_id":"gb-amp-10-mm-each-dntp-mixture","title":"GB-Amp™ 10mM dNTP Mixture","description":"\u003cp\u003eA mixture of dNTPs with 10mM each of dATP, dCTP, dGTP, dTTP. RNAse, DNAse-free, high purity. \u0026gt;2 year Stablity at -20 C. Can be used in polymerase and other enzyme catalyzed reactions, such as PCR, isothermal amplification and sequencing reactions.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/GB-Amp_10_mM_each_dNTP_Mixture.docx?v=1700752043\"\u003e\u003cspan\u003e\u003cimg height=\"46\" width=\"164\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\" data-mce-selected=\"1\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\"\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"0.5 ml","offer_id":39766537896036,"sku":"PCN-01-01","price":30.72,"currency_code":"CAD","in_stock":true},{"title":"2 ml","offer_id":39766537928804,"sku":"PCN-01-02","price":96.57,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_6fcee860-b060-462d-a604-77d0f575d68a.jpg?v=1706882362"},{"product_id":"gb-amp-25-mm-each-dntp-mixture","title":"GB-Amp™ 25mM dNTP Mixture","description":"\u003cp\u003eA mixture of dNTPs with 25 mM each of dATP, dCTP, dGTP, dTTP. RNAse, DNAse-free, high purity. \u0026gt;2 year Stablity at -20 C. Can be used in polymerase and other enzyme catalyzed reactions, such as PCR, isothermal amplification and sequencing reactions.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/dNTP_Mix-25_mM_each.docx?v=1700594064\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cimg height=\"42\" width=\"149\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\" data-mce-fragment=\"1\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" data-mce-selected=\"1\"\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"0.5 ml","offer_id":39766538027108,"sku":"RNK2401-01","price":38.4,"currency_code":"CAD","in_stock":true},{"title":"2 ml","offer_id":39766538059876,"sku":"RNK2401-02","price":131.68,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_c707bca6-9ba3-4a58-9cfa-fa073a36e067.jpg?v=1706882153"},{"product_id":"gb-amp-100-mm-dutp","title":"GB-Amp™ 100mM dUTP","description":"\u003cp\u003e Can be used in polymerase and other enzyme catalyzed reactions, such as PCR, isothermal amplification and sequencing reactions.\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"0.25 ml","offer_id":39766538289252,"sku":"PCN-01","price":54.87,"currency_code":"CAD","in_stock":true},{"title":"1 ml","offer_id":39766538322020,"sku":"PCN-02","price":197.52,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/226.jpg?v=1643677164"},{"product_id":"achievertm-dna-polymerase","title":"Achiever(TM) DNA Polymerase","description":"\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eAchiever(TM) DNA Polymerase allows amplification of the higher fidelity and longer templates than the single-enzyme formulations. It is also a better choice for amplifying complex template, such as GC-rich and secondary structure-harboring template. And it is suitable as a direct replacement for ordinary Taq Polymerase in most applications. In addition, Using Taq plus results in 3´-dA overhangs PCR products, which can be used in TA clone. \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eHigher fidelity: Its fidelity is four times higher than ordinary Taq polymerase.\u003cbr\u003eHigh yields: Suitable for amplifying complex template which is rich in GC or repeat sequence or that contains secondary structures.\u003cbr\u003eLong-range amplification by PCR. Large fragments as large as 20 kb for plasmid, phage DNA and 10 kb genomic DNA can be routinely amplified. \u003c\/p\u003e\n\u003ch2\u003e\n\u003cstrong\u003eQuality Control\u003c\/strong\u003e \u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests\u003c\/p\u003e\n\u003cp\u003ePlease see manual for product P201\u003c\/p\u003e\n\u003ch2\u003e\n\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/Achiever_TM_P201_Taq_Plus_DNA_Polymerase-GBS.pdf?v=1744128247\" rel=\"noopener\" target=\"_blank\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"178\" height=\"50\"\u003e\u003c\/a\u003e\u003cbr\u003eDefinition of Activity Unit\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eOne unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eStore all components at –20°C\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"250U","offer_id":39766538420324,"sku":"P201-01","price":43.89,"currency_code":"CAD","in_stock":true},{"title":"1000U","offer_id":39766538453092,"sku":"P201-02","price":153.63,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/AchieverDNAPolymerase.jpg?v=1669147313"},{"product_id":"100-mm-2-f-dntp-set","title":"100mM 2'-F-dNTP Set","description":"\u003cp\u003eA set of four 2'-F-dNTPs, with 100mM each of 2'-F-dATP, 2'-F-dCTP, 2'-F-dGTP, 2'-F-dUTP. RNAse, DNAse-free, high purity. \u0026gt;2 year Stability at -20 C. Can be used in RNA synthesis reactions to produce RNA with increased stability. \u003c\/p\u003e\n\u003cp\u003eIf you need other sizes, please  talk to us. \u003c\/p\u003e\n\u003cp\u003e\u0026gt;93% in purity. \u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"2mL","offer_id":39766538616932,"sku":"PCN03-01","price":3950.45,"currency_code":"CAD","in_stock":true},{"title":"4mL","offer_id":39766538649700,"sku":"PCN03-02","price":5706.22,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/228.jpg?v=1643677173"},{"product_id":"long-pcr-taq-master-mix-with-optimizer","title":"Long PCR Taq Master Mix with Optimizer","description":"\u003cp\u003e\u003cspan\u003eThis product is a 2X Long PCR reagent Mix, plus a separately supplied Long PCR DNA Polymerase. The mix contains dNTPs, Mg2+ and Buffer at optimal concentrations. This Optimzer format provides flexibility of optimizing Long DNA polymerase activity for each unique reaction and still the convenience that comes with master mixes. The Long Taq was shown to amplify long templates from λ phage genome of up to 20 kb. It is also a better choice for amplifying complex targets, such as GC-rich targets. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eLong PCR Taq DNA Polymerase,a combination of two thermostable DNA polymerases, Taq and Pfu, is a special formulation designed for amplifying large fragment. This specially formulated Long PCR Taq was shown to amplify long templates from λ phage genome of up to 20 kb. It is also a better choice for amplifying complex template, such as GC-rich template.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eLong PCR Taq is suitable as a direct replacement for ordinary Taq Polymerase in most applications. Using Long PCR Taq in your PCR reactions results in 3´-dA overhangs PCR products, \u003c\/span\u003ewhich can be used in TA clone\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e 5 x 1 ml is supplied. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eApplications\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• PCR amplification of DNA fragments any sizes around 5 kb\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• DNA labeling\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• DNA sequencing\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• PCR for cloning\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• High fidelity: three times fidelity of Taq DNA Polymerase.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Longer fragment: amplify long templates as long as 40kb.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Amplification of complex template (GC rich or repetitive sequence).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Generates 3'-dA and blunt end PCR products. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNote:\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e 10 x Long PCR Buffer is classical Long PCR Taq DNA Polymerase buffer, is good for long template especially above 10kb.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e 10 x Long PCR Buffer \u003cspan\u003eⅡ\u003c\/span\u003e is an alternative long PCR buffer . It is for better fidelity but may not be robust for longer templates above 10 kb.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNotes on cycling conditions\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e- Initial denaturation can be performed over an interval of 1~5 min at 95\u003cspan\u003e℃\u003c\/span\u003e depending on the GC content of template.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-Denaturation for 30 sec to 2 min at 94~95\u003cspan\u003e℃\u003c\/span\u003e is sufficient. If the amplified DNA has a very high GC content, denaturation time may be increased up to 4 min.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-Optimal annealing temperature is 5\u003cspan\u003e℃\u003c\/span\u003e lower than the melting temperature of primer-temperature DNA duplex. If nonspecific PCR products are obtained optimization of annealing temperature can be performed by increasing temperature stepwise by 2\u003cspan\u003e℃\u003c\/span\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-The number of PCR cycles depends on the amount of emplate DNA in the reaction mix and on the expected yield of the PCR products, 25-35 cycles are usually sufficient \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003efor the majority PCR reaction. Low amounts of starting template may require 40 cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-The time of the final extensi, on step can be extended for amplicons that will be cloned into T\/A vectors.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39766538748004,"sku":"P306","price":329.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/229.jpg?v=1643677180"},{"product_id":"gb-amp-taq-dna-polymerase","title":"GB-Amp™ Taq DNA Polymerase","description":"\u003cp\u003e\u003cstrong\u003e\u003cem\u003eOther sizes of this product are also available. Please inquire. \u003c\/em\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eGB-Amp™ \u003c\/span\u003eTaq DNA Polymerase is purified from E. coli. expressing a cloned Thermus aquaticus DNA polymerase gene. This enzyme has a 5' → 3' DNA polymerase and a 5' → 3' exonuclease activity but lacks a 3' → 5' exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. Taq DNA polymerase is heat-stable.\u003c\/p\u003e\n\u003cp\u003eContents\u003c\/p\u003e\n\u003cp\u003eTaq DNA Polymerase by default is packaged in 5 U\/μl.\u003c\/p\u003e\n\u003cp\u003e10×PCR Buffer with Mg++, \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003eApplications\u003cbr\u003eStandard PCR \u003cbr\u003eDNA labeling \u003cbr\u003eDNA sequencing \u003cbr\u003eNumerous applications for which a high-quality, thermostable DNA polymerase is required\u003cbr\u003e\u003cbr\u003eUnit Definition\u003cbr\u003eOne unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.\u003cbr\u003e\u003cbr\u003eStorage Buffer\u003c\/p\u003e\n\u003cp\u003e20mM TrisCl ( pH8.0), 100mM KCl, 3.2mM MgCl2 1mM DTT，0.1% Triton X-100 ,0.1% Tween20, 0.2mg\/ml BSA, 50% (v\/v) glycerol\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eNote\u003c\/p\u003e\n\u003cp\u003e-Recombinant Taq DNA Polymerase is the enzyme of choice for most PCR applications.\u003c\/p\u003e\n\u003cp\u003e-The half-life of enzyme is \u0026gt;40 minutes at 95°C.\u003c\/p\u003e\n\u003cp\u003e-The error rate of Taq DNA Polymerase in PCR is 2.2x10-5 errors per nt per cycle;\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003ethe accuracy (an inverse of the error rate) an average number of correct nucleotides incorporated before making an error, is 4.5x104.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e- Taq DNA Polymerase accepts modified nucleotides (e.g. biotin-, digoxigenin-, fluorescent-labeled nucleotides) as substrates for the DNA synthesis.Compatible with TA cloning – generates PCR products with 3’-dA overhangs.\u003c\/p\u003e\n\u003cp\u003e-Suitable for Taqman probe real time PCR\u003c\/p\u003e\n\u003cp\u003e-Recommendations with Template DNA in a 50μl reaction volume\u003c\/p\u003e\n\u003cp\u003e-recommended quantities of template per react\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"blob.png\" title=\"59b9137604a11.png\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/t\/6\/assets\/59b9137604a11.png?v=1643677186\"\u003e\u003c\/p\u003e\n\u003cp\u003eStore all components at –20°C\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/GBS-P101_Taq_DNA_Polymerase_Mg2_plus_buffer.pdf?v=1709224988\"\u003e\u003cspan\u003e\u003cspan style=\"text-decoration: underline;\"\u003e\u003cstrong\u003e\u003cimg data-mce-fragment=\"1\" height=\"52\" width=\"185\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\"\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"1000U","offer_id":39766538879076,"sku":"P101-01","price":105.42,"currency_code":"CAD","in_stock":true},{"title":"5000U","offer_id":39766538911844,"sku":"P101-02","price":419.13,"currency_code":"CAD","in_stock":true},{"title":"10,000U","offer_id":39766538944612,"sku":"P101-03","price":709.97,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/GB-AmpTaqDNAPolymerase.jpg?v=1669147237"},{"product_id":"gb-amp-hotstart-taq-2x-master-mix","title":"GB-Amp™ HotStart Taq 2X Master Mix","description":"\u003cdiv\u003eUsed for end-point PCR, this product is available in a version containing electrophoresis buffer and dye. It can be directly electrophoresed after the reaction. The 3' end of the PCR product, A base, can be directly cloned into the T vectors (e.g., our TA-cloning kits: \u003ca href=\"https:\/\/www.genebiosystems.com\/50-ta-cloning-kits\"\u003ehttps:\/\/www.genebiosystems.com\/50-ta-cloning-kits\u003c\/a\u003e)\u003c\/div\u003e\n\u003cp\u003eWhen using this product for qPCR reactions, reliable standard curve can be obtained in a wide range and accurate qualification of target genes can be achieved with high repeatability and reliability.\u003c\/p\u003e\n\u003cp\u003ePlease note: this product does not contain loading dye for gel electrophoresis, therefore, loading buffer with dye must be added before loading PCR products onto gels. \u003c\/p\u003e\n\u003cp\u003eFor the HotStart Taq 2 x Master Mix pre-spiked with loading dye, please refer to GB-Amp™ HotStart Taq 2x Master Mix (with Loading Dye) \u003clabel\u003eCat. #:\u003c\/label\u003e \u003cspan class=\"editable\"\u003eP2041b \u003ca href=\"https:\/\/www.genebiosystems.com\/pcr-reagents\/260802-hot-start-taq-2x-master-mix.html\"\u003ehttps:\/\/www.genebiosystems.com\/pcr-reagents\/260802-hot-start-taq-2x-master-mix.html\u003c\/a\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"editable\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003c\/h2\u003e\n\u003ch2\u003e\u003c\/h2\u003e","brand":"Gene Bio Systems","offers":[{"title":"1 ml","offer_id":39766539436132,"sku":"P2041","price":31.82,"currency_code":"CAD","in_stock":true},{"title":"5 ml","offer_id":39766539468900,"sku":"P2042","price":141.56,"currency_code":"CAD","in_stock":true},{"title":"10 ml","offer_id":39766539501668,"sku":"P2043","price":263.37,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_38f7efe8-962f-4d0a-b7fb-59ac67be3e78.jpg?v=1706709443"},{"product_id":"pcr-enhancing-reagent","title":"PCR Enhancing Reagent","description":"\u003cp\u003eThe PCR Enhancing Reagent is supplied in 10 x conc. Simply add the reagent in PCR reactions in the 1x-3x final concentration. We recommend that for each target, a series of reactions are set up to titrate the concentration of the PCR Enhancing Reagent in 1x, 2x, and 3 x concentrations.\u003c\/p\u003e\n\u003cp\u003eThe PCR Enhancing Reagent is also referred to as PCR Enhancer. \u003c\/p\u003e\n\u003cp\u003eThe effective melting temperature of primers in a reaction containing this reagent is reduced. Therefore, a decrease in annealing temperature of PCR is often required. \u003c\/p\u003e\n\u003cp\u003eWhat is this for? High GC content DNA targets or DNA targets with secondary structures. \u003c\/p\u003e\n\u003cp\u003eA low-cost way to troubleshoot difficult PCR amplification. Also try our high Robustness PCR enzymes, including the PaCeR. \u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"1mL","offer_id":41734492487780,"sku":"RNK3101","price":20.38,"currency_code":"CAD","in_stock":true},{"title":"5mL","offer_id":41734492520548,"sku":"RNK3102","price":83.09,"currency_code":"CAD","in_stock":true},{"title":"20mL","offer_id":41734492553316,"sku":"RNK3103","price":156.24,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/235.jpg?v=1643677207"},{"product_id":"2x-achiever-taq-master-mix-with-loading-dye","title":"2X Achiever™ Taq Master Mix (with loading dye)","description":"\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch2 data-end=\"175\" data-start=\"114\"\u003eProduct Details\u003c\/h2\u003e\n\u003cp class=\"\" data-end=\"563\" data-start=\"177\"\u003e\u003cstrong data-end=\"208\" data-start=\"177\"\u003e2X Achiever™ Taq Master Mix\u003c\/strong\u003e combines the high processivity of Taq polymerase with the superior fidelity of Pfu polymerase, delivering robust amplification performance even with complex templates. This dual-enzyme blend is ideal for amplifying longer fragments and GC-rich or structurally challenging DNA sequences—offering higher fidelity and greater yields than standard Taq alone.\u003c\/p\u003e\n\u003cp class=\"\" data-end=\"792\" data-start=\"565\"\u003eThe pre-mixed version includes a blue loading dye, allowing PCR products to be loaded directly onto an agarose gel for electrophoresis. The dye has been thoroughly validated to ensure it does not interfere with PCR performance.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli data-start=\"950\" data-end=\"1413\"\u003e\n\u003cstrong data-start=\"952\" data-end=\"971\"\u003eHigher Fidelity\u003c\/strong\u003e – 4x greater fidelity than standard Taq polymerase\u003cbr data-start=\"1022\" data-end=\"1025\"\u003e\u003cstrong data-start=\"1027\" data-end=\"1042\"\u003e\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"950\" data-end=\"1413\"\u003e\n\u003cstrong data-start=\"1027\" data-end=\"1042\"\u003eHigh Yields\u003c\/strong\u003e – Efficient amplification of GC-rich, repetitive, or structurally complex templates\u003cbr data-start=\"1126\" data-end=\"1129\"\u003e\u003cstrong data-start=\"1131\" data-end=\"1154\"\u003e\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"950\" data-end=\"1413\"\u003e\n\u003cstrong data-start=\"1131\" data-end=\"1154\"\u003eLong PCR Capability\u003c\/strong\u003e – Amplifies up to 10 kb from genomic DNA and up to 20 kb from plasmid or phage DNA\u003cbr data-start=\"1237\" data-end=\"1240\"\u003e\u003cstrong data-start=\"1242\" data-end=\"1267\"\u003e\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"950\" data-end=\"1413\"\u003e\n\u003cstrong data-start=\"1242\" data-end=\"1267\"\u003eTA Cloning-Compatible\u003c\/strong\u003e – Produces PCR products with 3’-dA overhangs, ideal for TA cloning\u003cbr data-start=\"1334\" data-end=\"1337\"\u003e\u003cstrong data-start=\"1339\" data-end=\"1360\"\u003e\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"950\" data-end=\"1413\"\u003e\n\u003cstrong data-start=\"1339\" data-end=\"1360\"\u003eConvenient Format\u003c\/strong\u003e – Includes blue loading dye for direct gel loading\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 data-start=\"1420\" data-end=\"1445\"\u003eQuality Assurance\u003c\/h2\u003e\n\u003cul data-start=\"1446\" data-end=\"1551\"\u003e\n\u003cli data-start=\"1446\" data-end=\"1488\" class=\"\"\u003e\n\u003cp data-start=\"1448\" data-end=\"1488\" class=\"\"\u003eFree from endo\/exonucleases and RNases\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1446\" data-end=\"1488\" class=\"\"\u003e\n\u003cp data-start=\"1448\" data-end=\"1488\" class=\"\"\u003eFunctionally tested in PCR for performance and reliability\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eStore all components at –20°C\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003ePlease see product protocols for \u003cspan\u003eP212-01 below:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/Achiever_P211-P212_2x_Master_Mix-GBS.pdf?v=1700579180\"\u003e\u003cimg height=\"49\" width=\"174\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\"\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"5mL","offer_id":39766541860964,"sku":"P212-01","price":164.61,"currency_code":"CAD","in_stock":true},{"title":"15mL","offer_id":39766541893732,"sku":"P212-02","price":384.07,"currency_code":"CAD","in_stock":true},{"title":"50mL","offer_id":39766541926500,"sku":"P212-03","price":986.52,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/image-removebg-achieverwithloadingdye.png?v=1680988362"},{"product_id":"fs-fast-dna-polymerase-2x-master-mix","title":"FS Fast DNA Polymerase 2X Master Mix","description":"\u003cp\u003e\u003cspan\u003eThe same high-performance Taq DNA polymerase, configured in a Master Mix format for your convenience. Fast Taq DNA polymerase is the latest generation Taq-based DNA polymerase. It possesses high amplification efficiency as does Taq polymerase, and fast elongation ability as does KOD polymerase, can be used in a variety of PCR. The FS\u003csup\u003eTM\u003c\/sup\u003e PCR Buffer, designed for FS\u003csup\u003eTM\u003c\/sup\u003e Taq DNA polymerase, can be used in fast amplification reaction. The elongation rate of FS\u003csup\u003eTM\u003c\/sup\u003e Taq DNA polymerase is more than twice the rate by regular Taq DNA polymerase, which shortens the amplification time at least by half , as fast as \u003cspan\u003e5s\/kb for simple sequence templates\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e Features\u003cbr\u003e Fast rate of elongation: elongation rate can reach to 3 kb\/min, more than twice the rate by regular Taq DNA polymerase;\u003cbr\u003e Highly thermostable : have a half-life of over 40 min at 95\u003cspan\u003e°\u003c\/span\u003eC incubation Generates 3'-dA overhangs PCR products.\u003cbr\u003e\u003cbr\u003e Applications\u003cbr\u003e Routine PCR\u003cbr\u003e PCR labeling\u003cbr\u003e PCR sequencing\u003cbr\u003e Generate PCR product for TA cloning\u003cbr\u003e\u003cbr\u003e Unit Definition\u003cbr\u003e One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70\u003cspan\u003e°\u003c\/span\u003eC using hering sperm DNA as substrate.\u003cbr\u003e\u003cbr\u003e Quality Control \u003cbr\u003e The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases is confirmed by appropriate quality tests\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFunctionally tested in amplification of a single-copy gene from human genomic DNA\u003cbr\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStore all components at \u003cspan\u003e–\u003c\/span\u003e20\u003cspan\u003e°\u003c\/span\u003eC\u003cbr\u003e\u003cbr\u003e Protocol for PCR\u003cbr\u003e All solutions should be thawed on ice, gently vortexed and briefly centrifuged.\u003cbr\u003e Add in a thin walled PCR tube on ice:\u003c\/span\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"1 ml","offer_id":39766543892580,"sku":"P2070","price":43.89,"currency_code":"CAD","in_stock":true},{"title":"5 ml","offer_id":39766543925348,"sku":"P2070","price":164.61,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/242.jpg?v=1643677274"},{"product_id":"rt-pcr-kit-cdna-synthesis-kit","title":"RT-PCR Kit ( cDNA synthesis kit)","description":"\u003cdiv class=\"body\"\u003e\n\u003cdiv class=\"w-container w-main\"\u003e\n\u003cdiv class=\"row\"\u003e\n\u003cdiv class=\"wrap-content-in w-system w-productcom\"\u003e\n\u003cdiv class=\"w-system-in clearfix\"\u003e\n\u003cdiv class=\"product-detail-wrap\"\u003e\n\u003cdiv class=\"w-com-content\"\u003e\n\u003cdiv class=\"product-maincon\"\u003e\n\u003cdiv class=\"product-maincon-in\"\u003e\n\u003cdiv class=\"product-descons\"\u003e\n\u003cdiv class=\"descon_item\" id=\"Tabitem234864\"\u003e\n\u003cp\u003e\u003cspan\u003eRT-PCR Kit is optimized to synthesize first-strand cDNA from purified poly(A)+ or total RNA. The RT-pcr kit for RT-PCR delivers increased cDNA yields, high sensitivity, and full-length transcripts in a convenient format. You get all of the components you need for successful first-strand cDNA synthesis, saving your time and ensuring your success with every experiment. The kit Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme is used to synthesize cDNA at a temperature range of 42-55\u003cspan\u003e°\u003c\/span\u003eC, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eApplications \u003cbr\u003eFirst strand cDNA synthesis for RT-PCR . \u003cbr\u003eConstruction of cDNA libraries. \u003cbr\u003eOne-step RT-PCR \u003cbr\u003ePrimer extension\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eComponents of the kit \u003c\/span\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eCompenent\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eµl\u003cstrong\u003e (20 rxns)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eµl\u003cstrong\u003e (100 rxns)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eM-MLV (200 U\/µl)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e20\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e100\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eRNAsine (40 U\/ µl)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e12\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e60\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eOligo (dT)15 (50 µM)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e20\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e100\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eRandom hexame (50 µM)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e20\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e100\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e5x First-strand buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e80\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e400\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eRnase-free ddH2O\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1 ml x 5\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003edNTP (10 mM each)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e50\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e200\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStorage\u003cbr\u003eAll components of the kit should be stored at -20\u003cspan\u003e°\u003c\/span\u003eC. Keep control RNA at -70\u003cspan\u003e°\u003c\/span\u003eC for longer storage.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eImportant notes \u003cbr\u003eAvoiding ribonuclease contamination \u003cbr\u003eRNA purity and integrity is essential for synthesis of full-length cDNA. RNA can be degraded RNase A, which is a highly stable contaminant found in any laboratory environment. All components of the kit have been rigorously tested to ensure that they are RNase free. To prevent contamination both the laboratory environment and all prepared solutions must be free of RNases. General recommendations to avoid RNase contamination:\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eDEPC-treat all tubes and pipette tips to be used in cDNA synthesis or use certified nuclease-free labware.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eWear gloves when handling RNA and all reagents, as skin is a common source of RNases. Change gloves frequently.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eUse RNase-free reagents, including high quality water (e.g., DEPC-treated Water).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eUse an RNase inhibitor to protect RNA from the activity of RNases.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eKeep all kit components tightly sealed when not in use. Keep all tubes tightly closed during the reverse transcription reaction.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003cspan\u003eTemplate RNA \u003cbr\u003eTotal cellular RNA isolated by standard methods is suitable for use with the kit. Purified RNA must be free of salts, metal ions, ethanol and phenol to avoid inhibiting the cDNA synthesis reaction. Trace contaminants can be removed by ethanol precipitation of the RNA followed by two washes of the pellet with cold 75% ethanol. For RT-PCR applications, template RNA must be free of DNA contamination. Prior to cDNA synthesis, RNA can be treated with DNase I to remove trace amounts of DNA (DNase I is not included in this kit). Always perform a control (RT-minus) reaction which includes all components for RT-PCR except for the reverse transcriptase enzyme.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eRNase H Digestion \u003cbr\u003eThe sensitivity of the PCR step can be increased (especially for long templates) by removing the RNA template from the cDNA:RNA hybrid molecule by digestion with RNase H after first-strand synthesis. Presence of RNase H during first-strand synthesis will degrade the template mRNA, resulting in decreased full-length cDNA synthesis and decreased yields of first-strand cDNA.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ePrimers \u003cbr\u003eSynthesis of first strand cDNA can be primed with either oligo(dT)15 primer, random primers or gene-specific primers.\u003cbr\u003eOligo(dT)15 primes cDNA synthesis from the poly(A) tail present at the 3\u003cspan\u003e’\u003c\/span\u003e-end of eukaryotic mRNA. Random Primers initiate cDNA synthesis from the total RNA population (rRNA and mRNA). Therefore, using random primers for first strand synthesis results in a greater complexity of the generated cDNA compared with the oligo(dT)15 primer. As a consequence, the sensitivity and specificity of subsequent PCR reactions may be reduced. However, there are several applications where it is beneficial to use random primers, such as cDNA synthesis using mRNAs without a poly(A) tail, or cDNA synthesis using poly(A)-enriched RNA samples.\u003cbr\u003eGene-specific primers are used to synthesize specifi c cDNA from a pool of total RNA or mRNA and must be obtained by the user.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFirst Strand cDNA synthesis procedure \u003cbr\u003eThe first strand cDNA reaction can be performed as an individual reaction or as a series of parallel reactions with different RNA templates. Therefore, the reaction mixture can be prepared by combining reagents individually or a master mix containing all of the components except template RNA can be prepared. Depending on the structure of the RNA template, separate steps for RNA denaturation and primer annealing may improve RT-PCR results.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eProtocol \u003cbr\u003eI. First strand cDNA synthesis\u003cbr\u003e1. Add the following reagents into a sterile, nuclease-free tube on ice in the indicated order:\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eTemplate RNA\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003ePoly (A) mRNA or specific RNA\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1-5 µg\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003ePrimer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eOligo (dT)15 Primer or Randome hexamer primer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1 µg\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eDEPC-treated water\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"2\"\u003e\n\u003cp\u003e\u003cspan\u003e To 12.4 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan\u003e\u003cbr\u003e2. Mix gently, centrifugebriefly and incubate at 70\u003cspan\u003e°\u003c\/span\u003eC for 5 min. Chill on ice, spin down and place the vial back on ice.\u003cbr\u003e3. Prepare the following cDNA Synthesis Mix, add the following components in the indicated order:\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e5x First-strand buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e4 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003edNTPs(10 mM each)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eRnasine\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e0.6 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eM-MLV\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4.Mix gently and centrifuge\u003cbr\u003e5.For oligo(dT)15, incubate for 60 min at 42\u003cspan\u003e°\u003c\/span\u003eC. For random hexamer primed synthesis, incubate for 60 min at 37\u003cspan\u003e°\u003c\/span\u003eC .\u003cbr\u003e6.Terminate the reaction by heating at 70\u003cspan\u003e°\u003c\/span\u003eC for 5 min. \u003cbr\u003eThe reverse transcription reaction product can be used immediately in second strand cDNA synthesis reactions or stored at -20\u003cspan\u003e°\u003c\/span\u003eC for less than a week. For longer storage, -70\u003cspan\u003e°\u003c\/span\u003eC is recommended.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eII. PCR Amplification of First Strand cDNA\u003cbr\u003eThe product of the first strand cDNA synthesis can be used directly in PCR or qPCR. The volume of first strand cDNA synthesis reaction mixture should not comprise more than 1\/10 of the total PCR reaction volume. Normally, 2 \u003cspan\u003eμ\u003c\/span\u003el of the first strand cDNA synthesis reaction mixture is used as template for subsequent PCR in 50 \u003cspan\u003eμ\u003c\/span\u003el total volume.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/R1011-R1012_RT-PCR_Kit_V1.0_1_-GBS.pdf?v=1700836884\"\u003e\u003cspan\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"160\" height=\"45\"\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Gene Bio Systems","offers":[{"title":"20 Preps","offer_id":39766544220260,"sku":"R1011","price":103.47,"currency_code":"CAD","in_stock":true},{"title":"100 Preps","offer_id":39766544253028,"sku":"R1012","price":354.29,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_ee950fd4-ff2b-49fa-b323-cba524f27fc4.jpg?v=1704394367"},{"product_id":"long-pcr-taq-polymerase-master-mix","title":"Long PCR Taq polymerase Master Mix","description":"\u003cp\u003e\u003cspan\u003eMaster mix Long PCR Taq DNA Polymerase, the same high-performing DNA polymerases, now in the master mix format to provide you with convenience of use. The master mix is a special formulation containing the Long PCR Taq DNA polymerase. The Long PCR Taq was shown to amplify long templates from λ phage genome of up to 20 kb. It is also a better choice for amplifying complex targets, such as GC-rich targets. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eJust add primers and template, your PCR reaction is ready to begin!\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eLong PCR Taq DNA Polymerase,a combination of two thermostable DNA polymerases, Taq and Pfu, is a special formulation designed for amplifying large fragment. This specially formulated Long PCR Taq was shown to amplify long templates from λ phage genome of up to 20 kb. It is also a better choice for amplifying complex template, such as GC-rich template.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eLong PCR Taq is suitable as a direct replacement for ordinary Taq Polymerase in most applications. Using Long PCR Taq in your PCR reactions results in 3´-dA overhangs PCR products, \u003c\/span\u003ewhich can be used in TA clone\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eApplications\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• PCR amplification of DNA fragments any sizes around 5 kb\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• DNA labeling\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• DNA sequencing\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• PCR for cloning\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• High fidelity: three times fidelity of Taq DNA Polymerase.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Longer fragment: amplify long templates as long as 40kb.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Amplification of complex template (GC rich or repetitive sequence).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Generates 3'-dA and blunt end PCR products. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNote:\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e 10xLong PCR Buffer\u003cspan\u003eⅠ\u003c\/span\u003eis classical Long PCR Taq DNA Polymerase buffer, is good for long template especially above 10kb.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e 10xLong PCR Buffer \u003cspan\u003eⅡ\u003c\/span\u003e is an alternatie long PCR buffer . It is for better fidelity but may not be robust for longer templates above 10kb.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eBasic PCR Protocol\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe following basic protocol serves as a general guideline and a starting point for any PCR amplification. Optimal reaction conditions \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e(incubation time and temperature, concentration of Taq DNA Polymerase, primers, Mg2+, and template DNA) vary and need to be optimized. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNotes on cycling conditions\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e- Initial denaturation can be performed over an interval of 1~5 min at 95\u003cspan\u003e℃\u003c\/span\u003e depending on the GC content of template.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-Denaturation for 30 sec to 2 min at 94~95\u003cspan\u003e℃\u003c\/span\u003e is sufficient. If the amplified DNA has a very high GC content, denaturation time may be increased up to 4 min.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-Optimal annealing temperature is 5\u003cspan\u003e℃\u003c\/span\u003e lower than the melting temperature of primer-temperature DNA duplex. If nonspecific PCR products are obtained optimization of annealing temperature can be performed by increasing temperature stepwise by 2\u003cspan\u003e℃\u003c\/span\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-The number of PCR cycles depends on the amount of emplate DNA in the reaction mix and on the expected yield of the PCR products, 25-35 cycles are usually sufficient \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003efor the majority PCR reaction. Low amounts of starting template may require 40 cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-The time of the final extensi, on step can be extended for amplicons that will be cloned into T\/A vectors.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"1mL","offer_id":39766544351332,"sku":"DNK300P-01","price":76.81,"currency_code":"CAD","in_stock":true},{"title":"3mL","offer_id":39766544416868,"sku":"DNK300P-02","price":197.52,"currency_code":"CAD","in_stock":true},{"title":"6mL","offer_id":39766544449636,"sku":"DNK300P-03","price":329.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/245.jpg?v=1643677287"},{"product_id":"fast-taq-blood-direct-mix","title":"Fast Taq Blood Direct Mix","description":"\u003cp\u003e\u003cspan\u003eFS Taq Mix Direct for Blood is a premixed, ready-to-use solution containing FS Taq DNA Polymerase, dNTPs, and all other PCR components, except DNA template and primers. FS Taq Mix Direct for Blood is specific for whole blood amplification. It contributes to fast, specific, sensitive and reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. The FS Mix (2X) B can be used with conventional PCR machines. This direct protocol makes the process of complicated and time-consuming DNA extraction unnecessary, and also minimize the risk of cross-contamination.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFS Taq DNA Polymerase is the latest generation Taq-based DNA polymerase. It possesses high amplification efficiency as does Taq polymerase, and fast elongation ability as does KOD polymerase, can be use in various kinds of PCR. The FS PCR Buffer is designed for FS Taq DNA polymerase, can be used in fast amplification reaction. FS Taq DNA polymerase has an elongation rate 2x higher than regular Taq DNA polymerase, and can shorten the amplification time by half. It has 5' to 3' polymerase activity but lacks 3' to 5' exonuclease activity, which results in a 3'-dA overhangs PCR product.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eContents\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2X Taq Mix Direct Mix B; Nuclease-free water\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNote: The product contains bromophenol blue for convenient gel loading of the PCR products. If you would like to leave it out, talk to us when you order the product.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFeatures\u003cbr\u003e\u003cspan\u003e•\u003c\/span\u003e Convenient: Direct for whole blood amplification PCR\u003cbr\u003e\u003cspan\u003e•\u003c\/span\u003e High yields of PCR products with minimal optimization\u003cbr\u003e\u003cspan\u003e•\u003c\/span\u003e Fast: saves time due to reduced number of pipetting steps \u003cbr\u003e\u003cspan\u003e•\u003c\/span\u003e Reproducible: lower contamination and pipetting error risk \u003cbr\u003e\u003cspan\u003e•\u003c\/span\u003e Higher sensitivity and fast compared to conventional Taq DNA polymerase\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eApplications\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e Amplification for Whole Blood\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e High throughput PCR\u003cbr\u003e\u003cspan\u003e•\u003c\/span\u003e Long and Complex template PCR\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003eUnit Definition\u003cbr\u003eOne unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70\u003cspan\u003e°\u003c\/span\u003eC using hering sperm DNA as substrate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eBasic PCR Protocol\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAll solutions should be thawed on ice, gently vortexed and briefly centrifuged.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1. Add in a thin walled PCR tube on ice:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cbr\u003eFor a total 50\u003cspan\u003eμ\u003c\/span\u003el reaction volume\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eReagent\/Sample\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eVolume (50 µl rxn)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eFinal concentration\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e10x FS PCR Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e25 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1x\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003ePrimer I\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eVariable\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e0.1-1 µM\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003ePrimer II\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eVariable\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e0.1-1 µM\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eBlood\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eVariable \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e0.2-2 µl*\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eWater\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eVariable to 50 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eN.A.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan\u003e*up to 5 \u003c\/span\u003e\u003cspan\u003eµl can be used. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eRecommendation for Blood Template in a 50\u003cspan\u003eμ\u003c\/span\u003el reaction volume is 0.1-1\u003cspan\u003eμ\u003c\/span\u003el.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. Gently vortex the sample and briefly centrifuge to collect all drops to the bottom of the tube.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3. Overlay the sample with mineral oil or add an appropriate amount of wax. This step may be omitted if the thermal cycler is equipped with a heated lid.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4. Preform PCR using the following thermal cycling conditions.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eInitial Denaturation\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e94°C \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e4 min\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e25-35 Cycles\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e94°C \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e55-68°C\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e72°C \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e30s\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e30s\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e20 s\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eFinal Extension\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e72°C \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e2 min\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"1 ml","offer_id":39766544908388,"sku":"P207A","price":109.74,"currency_code":"CAD","in_stock":true},{"title":"5 ml","offer_id":39766544941156,"sku":"P207A","price":329.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/247.jpg?v=1643677297"},{"product_id":"m-mlv-reverse-transcriptase","title":"M-MLV Reverse Transcriptase","description":"\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003eThe MMLV \u003c\/strong\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eReverse Transcriptase(short name MMLV) i is iolated from E.coli carrying rat leukemia virus pol gene consists of a single peptide, molecule weight 71kd.It possesses RNA and DNA depended polymerase activity and weak RNase H activity.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eConcentration: \u003c\/strong\u003e 200U\/μl\u003cbr\u003e\u003cstrong\u003eComponent : \u003c\/strong\u003e M-MLV （200U\/μl） 5×Buffer （with DTT）\u003cbr\u003e\u003cstrong\u003ePackage：\u003c\/strong\u003e Bulk\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFeatures:\u003c\/strong\u003e\u003cbr\u003eWeak RNaseH activity; High cDNA yield\u003cbr\u003e\u003cstrong\u003eStoreage: \u003c\/strong\u003e-20℃\u003cbr\u003eApplication：Synthesis of the first chain cDNA, cDNA Library construction, one-step RT-PCR, primer extention, 3′ and 5′RACE\u003cbr\u003e\u003cstrong\u003eSource:\u003c\/strong\u003e Recombination of E.coli containing Moloney murine leukemia virus reverse transcriptase gene from clone of Moloney murine .\u003cbr\u003e\u003cstrong\u003eUnit definition:\u003c\/strong\u003eOne unit is defined as the required enzyme incorporate 1 nm dNTP into a polynucleotide fraction in 10 min at 37℃, taking polyA﹒poly(dT)12-18 as template-primer.\u003cbr\u003e\u003cstrong\u003eProcedure:\u003c\/strong\u003e\u003cbr\u003e1. add the next reaction mixture to ice bath tube :\u003cbr\u003e1) template RNA\u003cbr\u003etotal RNA 0.1-5μg\u003cbr\u003eor total poly(A)+mRNA 0.1-0.5μg\u003cbr\u003eor unique RNA 0.01pg-0.5μg\u003cbr\u003e2) primer\u003cbr\u003eOligo(dT)18 (0.5μg\/μl) 1μl\u003cbr\u003eOr stochastic primer（0.2μg\/μl） 1μl\u003cbr\u003eOr sequence especially primer 20pmol\u003cbr\u003e3) RNase-free ddh2o : constant volume to 11μl\u003cbr\u003e2. Gently mix and water bath for 5 min in 70℃ and chill on ice.\u003cbr\u003e3. Put the tube into ice and add the next composition :\u003cbr\u003e5×Reaction Buffer 4μl\u003cbr\u003eRNase Inhibitor (40U\/μl) 0.5μl\u003cbr\u003edNTP Mix(10mmol\/L) 2μl\u003cbr\u003eadd water to 19ul ,gently mix and then water bath for 5 min in 37℃ ; or for 5 min in 25℃ for random primer \u003cbr\u003e4. Spin down for a few seconds. Add 1μl M-MLV RT（200U\/μl）\u003cbr\u003e5. Incubate at 42℃ for 60min(if use a random primer,first incubate for 10min in 25℃\u003cbr\u003e6. Inactivate at 70℃ for 10min.\u003cbr\u003e\u003cstrong\u003ePCR Reaction\u003c\/strong\u003e\u003cbr\u003e1. Transfer 10% volume of first reaction solution (2 μl ) to a proper PCR tube .\u003cbr\u003enote: the first reaction solution can be directly used as PCR template without purification ,the dosage is about 1-5μl. if excessively used ,the salt and Random primers in first reaction solution will restrain the activity of DNA polymerase .if purification needed ,it can follow the next :after reaction end of cDNA synthesis (step 6) , add RNase A in reaction system , 10 min in 37℃ ,use DP1501 recover cDNA .\u003cbr\u003e2. add next solution by order .\u003cbr\u003e5μl 10X PCR Buffer\u003cbr\u003e1μl 10mM dNTP mix\u003cbr\u003e1μl 10μM Primer #1 (customer supplied)\u003cbr\u003e1μl 10μM Primer #2 (p customer supplied)\u003cbr\u003exμl H20 (total reaction volume:49μl)\u003cbr\u003e1μl Taq DNA polymerase\u003cbr\u003e3. Mix thoroughly and add 50μl mineral oil to the surface of liquid.\u003cbr\u003e4. Amplified reaction : according to annealing temperature or gene copy number or technical parameter of Taq DNA polymerase , setting amplified condition , specify reference to specification of DNA polymerase ,the usually cycle number is 30-35\u003cbr\u003e5 Detect the product in agarose containing EB or another fluorescent dye.\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"5000U","offer_id":39766547136612,"sku":"R1041","price":87.8,"currency_code":"CAD","in_stock":true},{"title":"25000U","offer_id":39903278661732,"sku":"R1042","price":384.07,"currency_code":"CAD","in_stock":true},{"title":"50000U","offer_id":42761391505508,"sku":"R1043","price":702.31,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/SealingFilmGuide.png?v=1765475529"},{"product_id":"e-coli-uracil-dna-glycosylase-udg","title":"E. coli Uracil-DNA Glycosylase (UDG)","description":"\u003cp\u003e\u003cem\u003eE. coli\u003c\/em\u003e Uracil-DNA Glycosylase (UDG) is a recombinant enzyme purified from an \u003cem\u003eE. coli\u003c\/em\u003e strain that carries the UDG gene from \u003cem\u003eE. coli\u003c\/em\u003e.\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"5000 U","offer_id":39766547431524,"sku":"OTP-01","price":197.52,"currency_code":"CAD","in_stock":true},{"title":"25,000 U","offer_id":39766547464292,"sku":"OTP-01","price":877.88,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_309d961b-e467-4d9b-90a9-fc0f0f735868.jpg?v=1706882257"},{"product_id":"cold-sensitive-mutant-csm-taq-dna-pol","title":"Cold Sensitive Mutant (CSM) Taq DNA Pol","description":"\u003cp\u003e\u003cspan\u003eCSM Taq polymerases (Cold sensitive mutant Taq polymerases ) is a kind of HotStart Polymerase. Sourced from the mutant Ecoli. CSM Taq polymerase, unlike the monoclonal antibody based hot start Taq or chemical modified hot start Taq,Cold Taq is the cold sensitive mutant Taq. It retaisn the hot start ability throughout the whole amplification cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eCold sensitive mutant Taq polymerases are designed for Hot Start PCR, it is not active at low temperature. It offers excellent specificity and two-fold higher fidelity than wild-type Taq. It is designed for PCR with difficult templates such as GC-rich fragments and microsatellites. Cold sensitive mutant Taq polymerases are particularly well suited to primer extension of Single Nucleotide Polymorphism (SNP) markers.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eCold sensitive mutant Taq polymerases maintain excellent specificity and minimal background even in conditions designed for high yield. In fact, even on genomic templates, the enzyme can be used with MgCl2+concentrations as high as 10 mM.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eCold sensitive mutant Taq polymerases are capable of extending through difficult regions, e.g. regions, which include inverted tandem repeats and those with high amounts of secondary structure.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eCold sensitive mutant Taq polymerases work in a totally unique way, involving improved nucleotide selection at the active site, and a much lower rate of mis-match extension, meaning that only perfectly aligned primers will be extended. As a result, the enzyme can give even higher specificity than hot-start (manual or automatic) techniques without the need for inconvenient pre-incubation steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eConc. 5 U\/μl Package: Bulk\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStore at -20°C\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 Hot start PCR amplification\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2 Specific amplification of complex cDNA and genomic template, for amplification of difficult templates, such as GC-rich fragments and microsatellites\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3 Primer extension of SNP markers\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4 Amplification of genomic DNA targets up to 10 kb with high fidelity, specificity, and sensitivity\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5 Amplification from low copy number DNA template, high through-put Hot Start PCR with high specificity, sensitivity, and yield\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e6 Routine diagnostic Hot Start PCR requiring high reproducibility.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e7 Real-Time PCR\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e8 Multiple PCR\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e9 Generation of PCR products for TA cloning\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eQuality Control:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFunctional absence of double and single-stranded endonuclease activity; Purity\u0026gt;99% test by SDS gel electrophoresis; Each lot of CSM Taq DNA Polymerase is assayed for amplification from as little as 10 ng of human genomic DNA ; Retain full activity at room temperature for one week; No host DNA residue.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"1000 U","offer_id":39766549921892,"sku":"DNK2101","price":109.74,"currency_code":"CAD","in_stock":true},{"title":"5000 U","offer_id":39766549954660,"sku":"DNK2101","price":329.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/274.jpg?v=1643677433"},{"product_id":"gb-amp-100-mm-dntp-set","title":"GB-Amp™ 100mM dNTP Set","description":"\u003cp\u003e Can be used in polymerase and other enzyme catalyzed reactions, such as PCR, isothermal amplification and sequencing reactions. Available in two sizes, 4x0.25 ml and 4 x 1 ml.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/dNTP_set.docx?v=1700594142\"\u003e\u003cspan\u003e\u003cimg height=\"35\" width=\"124\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\"\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"1 ml","offer_id":39766555295844,"sku":"PCN-04-01","price":101.61,"currency_code":"CAD","in_stock":true},{"title":"4 ml","offer_id":39766555328612,"sku":"PCN-04-02","price":355.62,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_86d0e14b-dd88-4751-a094-d4c76a66e676.jpg?v=1706882328"},{"product_id":"gb-amp-hotstart-taq-dna-polymerase","title":"GB-Amp™ HotStart Taq DNA polymerase","description":"\u003cp\u003eDescription\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eGB-Amp™ HotStart Taq DNA polymerase\u003c\/span\u003e is a hot-start polymerase with chemical modification, which brings higher specificity by reducing non-specific products as the enzyme activity is temperature-dependent and is inhibited at room temperature. The amplification length and speed can reach to 5 kb (simple template) and 0.5 kb\/min separately. Hotstart Taq has 5’-3’ polymerase activity, but no 3’-5’ exonuclease activity. The products of Taq plus have overhanged dA at 3’-end.\u003c\/p\u003e\n\u003cp\u003eComponents: Hotstart Taq DNA polymerase (5U\/ul); 10 Hotstart Buffer (including Mg), 6 x gel loading buffer\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eStable for 2 years at -20°C\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eGB-Amp™ HotStart Taq DNA polymerase\u003c\/span\u003e has zero animal source pollution by being produced with advanced chemical modification. And it is much more stable than antibody-modified hot-start polymerase. Its efficiency is higher than most chemical-modified polymerase and the initial-denaturation time can be reduced to 3 minutes.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eUnit Definition\u003c\/p\u003e\n\u003cp\u003eOne enzyme unit (U) refers to the amount of enzyme needed for intaking 10 nmol nucleotides when using activated salmon sperm DNA as template\/primer, at 72 ℃, in 30 minutes\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eQuality control\u003c\/p\u003e\n\u003cp\u003eThe absence of endonuclease or exonuclease is confirmed by appropriate quality tests. PCR detects no host residual DNA, and it can effectively amplify the single-copy gene of human genome. There is no significant change about the amplification activity after one week at room temperature.\u003c\/p\u003e\n\u003cp\u003eStorage Buffer\u003c\/p\u003e\n\u003cp\u003e200 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Tween 20, 50% Glycerol, 0.5% Triton X -100\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eNotes\u003c\/p\u003e\n\u003cp\u003e1 This product adopts improved chemical modification technology, so it relies on temperature to activate the polymerase activity, which can effectively inhibit non-specific bindings, and the reaction system can be configured at room temperature.\u003c\/p\u003e\n\u003cp\u003e2 This Hotstart Taq DNA polymerase has the deoxidizing nucleotide transfer activity, so at the 3’-end of the PCR product is usually added to an extra deoxygenated adenosine nucleoside.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eEndodeoxyribonuclease Assay\u003c\/p\u003e\n\u003cp\u003eNo detectable conversion of covalently closed circular DNA to a nicked DNA was observed after incubation of 10U \u003cspan\u003eGB-Amp™ HotStart Taq DNA polymerase\u003c\/span\u003e with 1μg pBR322 DNA for 4 hours at 37°C and 70°C.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eExodeoxyribonuclease Assay\u003c\/p\u003e\n\u003cp\u003eNo detectable degradation of lambda DNA-HindIII fragments was observed after incubation of 10U \u003cspan\u003eGB-Amp™ HotStart Taq DNA polymerase\u003c\/span\u003e with 1μg digested DNA for 4 hours at 37°C and 70°C.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eRibonuclease Assay\u003c\/p\u003e\n\u003cp\u003e0% of the total radioactivity was released into trichloroacetic acid-soluble fraction after incubation of 10U \u003cspan\u003eGB-Amp™ HotStart Taq DNA polymerase\u003c\/span\u003e with 1μg E.coli [3H]-RNA (40000cpm\/μg) for 4 hours at 37°C and 70°C.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eQ\u0026amp;A\u003c\/p\u003e\n\u003cp\u003eWhy the specificity of \u003cspan\u003eGB-Amp™ HotStart Taq DNA polymerase\u003c\/span\u003ee is higher than ordinary Taq DNA polymerase?\u003c\/p\u003e\n\u003cp\u003eThe Hotstart Taq DNA polymerase is a chemically modified Taq DNA polymerase, which activity is completely inhibited at room temperature and it is activated at high temperature in a very short time. Low temperature inhibition \u0026amp; high temperature activation keep the non-specific reactions, i.e., primer dimer formation and off-target amplification low, and thus the specificity high.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eAre the products of Hotstart Taq DNA polymerase compatible to TA cloning?\u003c\/p\u003e\n\u003cp\u003eYes. Because the products have 3’-dA protruding ends.\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"250U","offer_id":39766555689060,"sku":"P1041","price":105.41,"currency_code":"CAD","in_stock":true},{"title":"1000U","offer_id":39766555721828,"sku":"P1042","price":381.02,"currency_code":"CAD","in_stock":true},{"title":"3000U","offer_id":39766555754596,"sku":"P1043","price":889.06,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_45a577d3-2d04-49d1-83f1-c8e892d4d331.jpg?v=1706709365"},{"product_id":"fs-fast-dna-polymerase","title":"FS Fast DNA Polymerase","description":"\u003cp\u003e\u003cspan\u003eFS\u003csup\u003eTM\u003c\/sup\u003e Taq DNA polymerase is the latest generation Taq-based DNA polymerase. It possesses high amplification efficiency as does Taq polymerase, and fast elongation ability as does KOD polymerase, can be used in a variety of PCR. The FS\u003csup\u003eTM\u003c\/sup\u003e PCR Buffer, designed for FS\u003csup\u003eTM\u003c\/sup\u003e Taq DNA polymerase, can be used in fast amplification reaction. The elongation rate of FS\u003csup\u003eTM\u003c\/sup\u003e Taq DNA polymerase is as fast as \u003cspan\u003e5s\/kb for simple templates\u003c\/span\u003e, which shortens the amplification time at least by half .\u003cbr\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eContents\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFS\u003csup\u003eTM\u003c\/sup\u003e Taq DNA Polymerase, 10x FS\u003csup\u003eTM\u003c\/sup\u003e PCR Buffer, 6 x gel loading buffer\u003cbr\u003e\u003cbr\u003e Features\u003cbr\u003e Fast rate of elongation: elongation rate can reach to 3 kb\/min, more than twice the rate by regular Taq DNA polymerase;\u003cbr\u003e Highly thermostable : have a half-life of over 40 min at 95\u003cspan\u003e°\u003c\/span\u003eC incubation Generates 3'-dA overhangs PCR products.\u003cbr\u003e\u003cbr\u003e Applications\u003cbr\u003e Routine PCR\u003cbr\u003e PCR labeling\u003cbr\u003e PCR sequencing\u003cbr\u003e Generate PCR product for TA cloning\u003cbr\u003e\u003cbr\u003e Unit Definition\u003cbr\u003e One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70\u003cspan\u003e°\u003c\/span\u003eC using hering sperm DNA as substrate.\u003cbr\u003e\u003cbr\u003e Quality Control \u003cbr\u003e The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases is confirmed by appropriate quality tests\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFunctionally tested in amplification of a single-copy gene from human genomic DNA\u003cbr\u003e\u003cbr\u003e Storage Buffer\u003cbr\u003e 20 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5 %TW 20, 0.5 % NP 40, 50 % Glycerol\u003cbr\u003e\u003cbr\u003e 10X FS\u003csup\u003eTM\u003c\/sup\u003e PCR Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e200 mM Tris-Cl(PH 8.8), 100 mM KCl, 100mM (NH4)2SO4, 16 mM MgSO4, 1% Triton-X-100.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStore all components at \u003cspan\u003e–\u003c\/span\u003e20\u003cspan\u003e°\u003c\/span\u003eC\u003cbr\u003e\u003cbr\u003e Protocol for PCR\u003cbr\u003e All solutions should be thawed on ice, gently vortexed and briefly centrifuged.\u003cbr\u003e Add in a thin walled PCR tube on ice:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cbr\u003e For a total 50\u003cspan\u003eμ\u003c\/span\u003el reaction volume\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eReagent\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eVolume (50 µl rxn)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eFinal concentration\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e10x PCR Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e5 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1x\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003edNTPs(10 mM each)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e0.2 mM each\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003ePrimer I\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eVariable\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e0.4-1 µM\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003ePrimer II\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eVariable\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e0.4-1 µM\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eFast DNA polymerase (5U\/µl)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e0.25-0.5 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1.25-2.5U\/50 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eWater \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eVariable to 50 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eN.A.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003eGently vortex the sample and briefly centrifuge to collect all drops to the bottom of the tube.\u003cbr\u003e\u003cspan\u003e·\u003c\/span\u003eOverlay the sample with mineral oil or add an appropriate amount of wax. This step may be omitted if the thermal cycler is equipped with a heated lid.\u003cbr\u003e\u003cspan\u003e·\u003c\/span\u003ePlace samples in a thermocycler and start the program.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003ePCR Cycling Protocol\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eInitial Denaturation\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e94°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e3 min\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e25-35 Cycles\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e94°C\u003c\/p\u003e\n\u003cp\u003e55-68°C\u003c\/p\u003e\n\u003cp\u003e72°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e30s\u003c\/p\u003e\n\u003cp\u003e30s\u003c\/p\u003e\n\u003cp\u003e10-60 s\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eFinal Extension\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e72°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e2 min\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/FS_Fast_DNA_Polymerase_P1071-user_manual.pdf?v=1700751972\"\u003e\u003cspan\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"164\" height=\"46\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" data-mce-selected=\"1\"\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"250 U","offer_id":39766555885668,"sku":"P1071","price":38.1,"currency_code":"CAD","in_stock":true},{"title":"1000 U","offer_id":39766555918436,"sku":"P1073","price":127.01,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/322.jpg?v=1643677674"},{"product_id":"long-pcr-taq-dna-polymerase","title":"Long PCR Taq DNA polymerase","description":"\u003cp\u003e\u003cspan\u003eLong PCR Taq DNA Polymerase,a combination of two thermostable DNA polymerases, Taq and Pfu, is a special formulation designed for amplifying large fragment. This specially formulated Long PCR Taq was shown to amplify long templates from λ phage genome of up to 20 kb. It is also a better choice for amplifying complex template, such as GC-rich template.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eLong PCR Taq is suitable as a direct replacement for ordinary Taq Polymerase in most applications. Using Long PCR Taq in your PCR reactions results in 3´-dA overhangs PCR products, \u003c\/span\u003ewhich can be used in TA clone\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eContents: Long PCR Taq DNA Polymerase, PCR Enhancer, 6x gel loading buffer, 10X Long PCR Taq Buffer \u003cspan\u003eⅠ\u003c\/span\u003e with Mg2+, 10X Long PCR Taq Buffer \u003cspan\u003eⅡ\u003c\/span\u003e with Mg2+\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eApplications\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• PCR amplification of DNA fragments any sizes around 5 kb\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• DNA labeling\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• DNA sequencing\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• PCR for cloning\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eUnit Definition\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eOne unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmoles of dNTP’s into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStorage Buffer \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e20mM TrisCl ( pH8.0), 100mM KCl, 3mM MgCl2 1mM DTT\u003cspan\u003e，\u003c\/span\u003e0.1% NP-40, 0.1% Tween20, 0.2mg\/ml BSA, 50% (v\/v) glycerol\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e10X Long PCR Taq Buffer \u003cspan\u003eⅠ\u003c\/span\u003e with Mg2+\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e500mM Tris-HCl pH 8.8\u003cspan\u003e，\u003c\/span\u003e160mM (NH4)2SO4 \u003cspan\u003e，\u003c\/span\u003e25mM MgCl2 \u003cspan\u003e，\u003c\/span\u003e1% Triton X-100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e10X Long PCR Taq Buffer \u003cspan\u003eⅡ\u003c\/span\u003e with Mg2+\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e200mM Tris-HCl PH8.8\u003cspan\u003e，\u003c\/span\u003e100mM KCl\u003cspan\u003e，\u003c\/span\u003e100mM (NH4)2SO4\u003cspan\u003e，\u003c\/span\u003e16mM MgSO4\u003cspan\u003e，\u003c\/span\u003e1% Tritonx-100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• High fidelity: three times fidelity of Taq DNA Polymerase.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Longer fragment: amplify long templates as long as 40kb.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Amplification of complex template (GC rich or repetitive sequence).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Generates 3'-dA and blunt end PCR products. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNote:\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e 10xLong PCR Buffer\u003cspan\u003eⅠ\u003c\/span\u003eis classical Long PCR Taq DNA Polymerase buffer, is good for long template especially above 10kb.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e 10xLong PCR Buffer \u003cspan\u003eⅡ\u003c\/span\u003e is an alternatie long PCR buffer . It is for better fidelity but may not be robust for longer templates above 10kb.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003e• Users may choose compare the two buffers for different template. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e Basic PCR Protocol \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe following basic protocol serves as a general guideline and a starting point for any PCR amplification. Optimal reaction conditions \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e(incubation time and temperature, concentration of Taq DNA Polymerase, primers, Mg2+, and template DNA) vary and need to be optimized. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e 1. Add the following components to a sterile microcentrifuge tube sitting on ice:\u003cbr\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eReagent\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eVolume (50 µl rxn)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eFinal concentration\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e10x PCR Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e5 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1x\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003edNTPs(10 mM each)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e0.2 mM each\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003ePrimer I\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eVariable\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e0.4-1 µM\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003ePrimer II\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eVariable\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e0.4-1 µM\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eLong PCR DNA polymerase (5U\/µl)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e0.25-0.5 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1.25-2.5U\/50 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eWater\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eVariable to 50 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eN.A.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. Mix contents of tube. Cap tubes and centrifuge briefly to collect the contents to the bottom. When using a thermal cycler that does not\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e contain a heated lid, overlay the reaction mixture with 25 μl mineral oil. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3. Perform 25-35 cycles of PCR amplification as follows:\u003cbr\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eInitial Denaturation\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e94°C \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e3 min\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e25-35 Cycles\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e94°C \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e55-68°C\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e72°C \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e30s\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e30s\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1-10 mins\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eFinal Extension\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e72°C \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e10 min\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan\u003e4. Incubate for an additional 10 min at 72°C and maintain the reaction at 4°C. The samples can be stored at -20°C until use. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5. Analyze the amplification products by agarose gel electrophoresis and visualize by ethidium bromide staining. Use appropriate molecular weigh standards.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNotes on cycling conditions\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e- Initial denaturation can be performed over an interval of 1~5 min at 95\u003cspan\u003e℃\u003c\/span\u003e depending on the GC content of template.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-Denaturation for 30 sec to 2 min at 94~95\u003cspan\u003e℃\u003c\/span\u003e is sufficient. If the amplified DNA has a very high GC content, denaturation time may be increased up to 4 min.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-Optimal annealing temperature is 5\u003cspan\u003e℃\u003c\/span\u003e lower than the melting temperature of primer-temperature DNA duplex. If nonspecific PCR products are obtained optimization of annealing temperature can be performed by increasing temperature stepwise by 2\u003cspan\u003e℃\u003c\/span\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-The number of PCR cycles depends on the amount of emplate DNA in the reaction mix and on the expected yield of the PCR products, 25-35 cycles are usually sufficient \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003efor the majority PCR reaction. Low amounts of starting template may require 40 cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-The time of the final extensi, on step can be extended for amplicons that will be cloned into T\/A vectors.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"250U","offer_id":39766556278884,"sku":"P1061","price":101.61,"currency_code":"CAD","in_stock":true},{"title":"1000U","offer_id":39766556311652,"sku":"P1063","price":355.62,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/324.jpg?v=1643677684"},{"product_id":"gb-amp-qpcr-probe-master-mix","title":"GB-Amp™ qPCR Probe Master Mix","description":"\u003cdiv class=\"body\"\u003e\n\u003cdiv class=\"w-container w-main\"\u003e\n\u003cdiv class=\"row\"\u003e\n\u003cdiv class=\"wrap-content-in w-system w-productcom\"\u003e\n\u003cdiv class=\"w-system-in clearfix\"\u003e\n\u003cdiv class=\"product-detail-wrap\"\u003e\n\u003cdiv class=\"w-com-content\"\u003e\n\u003cdiv class=\"product-maincon\"\u003e\n\u003cdiv class=\"product-maincon-in\"\u003e\n\u003cdiv class=\"product-descons\"\u003e\n\u003cdiv id=\"Tabitem234864\" class=\"descon_item\"\u003e\n\u003cp\u003eWhen using this product for qPCR reactions, reliable standard curve can be obtained in a wide range and accurate qualification of target genes can be achieved with high repeatability and reliability.\u003c\/p\u003e\n\u003cp\u003eContent\u003c\/p\u003e\n\u003ctable style=\"width: 572px;\" cellspacing=\"0\" cellpadding=\"1\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 315.614px;\"\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e  Components\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 117.386px;\"\u003e\n\u003cp\u003ePCR-04-01\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e(125 rxn \/ 20 µl reaction)\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 129px;\"\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003ePCR-04-02\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e(500 rxn \/ 20 µl reaction)\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 20px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 315.614px;\"\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eGB-Amp™ qPCR Probe Master Mix \u003cspan\u003ea\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 117.386px;\"\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e1.25 ml\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 129px;\"\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e1.25 ml × 4\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 20px;\" rowspan=\"3\" colspan=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 315.614px;\"\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e  50 X ROX Reference Dye 1 \u003cspan\u003eb\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 117.386px;\"\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e50 µl\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 129px;\"\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e200 μl\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 315.614px;\"\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e  50 X ROX Reference Dye 2 \u003cspan\u003eb\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 117.386px;\"\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e50 µl\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 129px;\"\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e200 μl\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003ea. Contains dNTP Mix, Mg2+, and our HotStart DNA Polymerase.\u003cbr\u003eb. Used to rectify the error of fluorescence signals between different wells.\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/GB-Amp_Probe_qPCR_Mix.pdf?v=1700752247\"\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cimg data-mce-fragment=\"1\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"164\" height=\"46\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" data-mce-selected=\"1\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Gene Bio Systems","offers":[{"title":"1.25mL","offer_id":39766556409956,"sku":"PCR-04-01","price":137.84,"currency_code":"CAD","in_stock":true},{"title":"5mL","offer_id":39766556442724,"sku":"PCR-04-02","price":481.97,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/probe.jpg?v=1702051786"},{"product_id":"redscripttm-m-mlv-reverse-transcriptase-h","title":"REDscript(TM) M-MLV Reverse Transcriptase (H-)","description":"\u003cp\u003e\u003cstrong\u003eREDScript(TM)\u003c\/strong\u003e \u003cstrong\u003eM-MLV Reverse Transcriptase\u003c\/strong\u003e, encoded by Moloney Murine Leukemia Virus (\u003cstrong\u003eMMLV RT\u003c\/strong\u003e) is an RNA-dependent DNA polymerase that synthesizes the complementary DNA (cDNA) first strand from a single-stranded RNA template to which a primer has been hybridized. M-MLV RT will also extend primers hybridized to single-stranded DNA. Second strand cDNA synthesis can be achieved from some mRNA templates without an additional DNA polymerase. The difference between this to the general M-MLV RT is that the capacity to endure the heat is enhanced. It can remain the 100% activity at 50℃, it can also keep more than 75% activity at even at 55℃.\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"Thermal Stable M-MLV Reverse RH-\" src=\"http:\/\/www.mebep.com\/uploads\/allimg\/110608\/1-11060Q64Z1224.jpg\"\u003e\u003cimg alt=\"Thermal Stable M-MLV\" src=\"http:\/\/www.mebep.com\/uploads\/allimg\/110608\/1-11060QA021I1.jpg\"\u003e\u003c\/p\u003e\n\u003cp\u003eUpper panel: Template: Mouse total RNA, Reverse transcription at 50℃\u003c\/p\u003e\n\u003cp align=\"left\"\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eLane 1-2: \u003cstrong\u003eREDscript(TM) M-MLV\u003c\/strong\u003e\u003cstrong\u003e 200U \u003c\/strong\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"left\"\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eLane 3-4 :invitrogen SuperScript® III \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"left\"\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eLane 5-6:Takara m-mlv \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"left\"\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eLane M: Marker \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"left\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eLower panel: Template: Mouse total RNA, Reverse transcription at 55℃\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eLane 1-2:Takara m-mlv\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eLane 3-4: \u003cstrong\u003eREDscript(TM) M-MLV\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e Lane 5-6:200U invitrogen SuperScript® III\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"left\"\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eLane M: Marker \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003eSource: Recombination of E.coli containing Moloney murine leukemia virus reverse transcriptase gene from clone of Moloney murine.\u003cbr\u003eConcentration: 200U\/μl\u003cbr\u003eComponent: M-MLV （200U\/μl）\u003cbr\u003e 5×Buffer （with DTT）\u003cbr\u003e Package： \u003cstrong\u003eBulk\u003c\/strong\u003e\u003cbr\u003eFeatures: Weak RNaseH activity High cDNA yield\u003cbr\u003eStoreage: -20℃\u003c\/p\u003e\n\u003cp\u003eUnit Definition: One unit of \u003cstrong\u003eREDscript(TM) M-MLV\u003c\/strong\u003e RT catalyzes\u003cbr\u003ethe incorporation of 1 nmol of dTTP into acidinsoluble material in 10 minutes at 37℃ using oligo(dT)12-18-primed poly(A)n as a template.\u003c\/p\u003e\n\u003cp\u003eApplications: The first-strand cDNA synthesis; RT-PCR.\u003c\/p\u003e\n\u003cp\u003eStorage Buffer: 20 mM Tris-HCl (pH7.5), 200 mM NaCl，0.25 mM EDTA, 0.01% NP-40(v\/v)，2.5 mM DTT,50% glycerol (v\/v).\u003cbr\u003e5X Reaction Buffer:\u003cbr\u003e[5×RT Buffer] 250mM Tris-HCl (pH 8.3), 15mM MgCl2，375 mM KCl，50mM DTT.\u003cbr\u003eRecommended Reaction Conditions:\u003cbr\u003eThe first-strand cDNA synthesis\u003cbr\u003e1) Add the following reagents to a RNase free PCR tube at room temperature add the MMLV RT last.\u003cbr\u003eOligo dT12-18 (1μg\/μl) or random primer (50-250ng) 1μl\u003cbr\u003eTotal RNA (10ng-5μg) or mRNA(1-500ng) xμl\u003cbr\u003edNTP (10mM each) 1μl\u003cbr\u003eDEPC ddH2O (14-x)μl\u003cbr\u003e2) Gently mix and incubate 10 Min at 70℃ then chill on ice for 2-10min.\u003cbr\u003e3) Centrifuge for a few seconds then Put the tube into ice and add the next composition :\u003cbr\u003e5×RT Buffer 4μl\u003cbr\u003eRNasin (40U\/μl) 1μl\u003cbr\u003e5) Gently mix and incubate at 50℃ for 2 Min（Oligo dT12-18 or sequence especially primer）\u003cbr\u003eor at 25℃ for 10 min for the random primer.\u003cbr\u003e6) Centrifuge for a few seconds. Add 1μl \u003cstrong\u003eREDscript(TM) \u003c\/strong\u003eM-MLV RT（200U\/μl）Incubate at 50℃ for 50min.\u003cbr\u003e7) Inactivate at 70℃ for 10min then get the cDNA.\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"5000 U","offer_id":39766556606564,"sku":"PCR-05","price":127.01,"currency_code":"CAD","in_stock":true},{"title":"25,000 U","offer_id":39766556639332,"sku":"PCR-05","price":533.43,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/326.jpg?v=1643677692"},{"product_id":"ribonuclease-inhibitor","title":"Ribonuclease Inhibitor","description":"\u003cp\u003eRibonuclease Inhibitor, Human Placenta is a recombinant human placental protein with specifically inhibits broad-spectrum RNase such as RNase A, RNase B and RNase C. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H. It does not inhbit Taq DNA polymerase and T7 RNA Polymerase. The 50kDa protein exerts its inhibitory effect by noncovalently binding to RNases at 1:1 ratio. The Ki value for binding of Rnase Inhibitor, Human Placenta to RNase is approximately 10(-14)M.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFeature:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eImproved resistance to oxidation, compared to human\/porcine Rnase inhibitor\u003c\/p\u003e\n\u003cp\u003eIdeal for reactions where low DTT concentrations are required (e.g. Real-time PCR)\u003c\/p\u003e\n\u003cp\u003eSource An E.coli strain that carries Ribonuclease Inhibitor gene from human placenta.\u003c\/p\u003e\n\u003cp\u003eVolume activity 40U\/ul\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003eSource An E.coli strain that carries Ribonuclease Inhibitor gene from human placenta. Recombinant\u003c\/p\u003e\n\u003cp\u003eVolume activity 40U\/ul\u003c\/p\u003e\n\u003cp\u003eUnit Definition of Rnase in One unit is defined as the amount of Recombinant \u003cem\u003eRibonuclease Inhibitor\u003c\/em\u003e required inhibit the activity of 5ng of ribonuclease A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2’3’-cyclic monophosphate by ribonuclease A.\u003c\/p\u003e\n\u003cp\u003eStorage Temperature -20°C\u003c\/p\u003e\n\u003cp\u003eStorage Buffer 20mM HEPES-KOH(PH7.6), 50mM KCl, 8mM DTT, 50%(v\/v) glycerol.\u003c\/p\u003e\n\u003cp\u003eQuality Control: Absence of Endonuclease 1μg of Lambda DNA is incubated with 200 units of Ribonuclease Inhibitor for 16 hour at 37°C. Following incubation, Lambda DNA is visualized as intact on an ethidium bromide-stained agarose gel to verify the absence of visible Endonuclease. RNase Inhibitor, Murine is a 50 kDa recombinant protein of murine origin. The inhibitor specifically inhibits RNases A, B and C. It inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from\u003cem\u003e Aspergillus\u003c\/em\u003e. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor is used with \u003ca href=\"http:\/\/www.mebep.com\/a\/Products\/Enzyme\/Taq_DNA_Polymerase\/2011\/0505\/Taq_DNA_Polymerase.html\"\u003e\u003cem\u003eTaq\u003c\/em\u003e DNA Polymerase\u003c\/a\u003e, AMV or\u003ca href=\"http:\/\/www.mebep.com\/a\/Products\/Enzyme\/M-MLV_Reverse_Transcriptase\/2011\/0505\/Thermal_stable_M-MLV_Reverse.html\"\u003e M-MuLV Reverse Transcriptases\u003c\/a\u003e, or Phage RNA Polymerases (SP6, T7, or T3).\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"2000 U","offer_id":39766556770404,"sku":"R201-01","price":54.43,"currency_code":"CAD","in_stock":true},{"title":"25,000 U","offer_id":39766556803172,"sku":"R201-02","price":604.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/327.jpg?v=1643677697"},{"product_id":"master-max-1-2-3-universal-pcr-beads","title":"MASTER MAX™ 1-2-3™ UNIVERSAL PCR BEADS","description":"\u003ch2\u003eMASTER MAX™ 1-2-3™ UNIVERSAL PCR BEADS\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Overview:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eThe MASTER MAX™ 1-2-3™ Universal PCR Beads are meticulously formulated, pre-mixed, and pre-dispensed reagents designed to facilitate efficient and reliable polymerase chain reaction (PCR) amplifications. These beads encompass all essential components, including recombinant HotStart DNA polymerase, deoxynucleotide triphosphates (dNTPs), and a reaction buffer, optimized specifically for endpoint PCR applications. The beads are conveniently available in 0.2 ml 8-strip tubes, making them ideal for a wide array of research applications.\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eEnhanced Stability\u003c\/strong\u003e: The beads are stable at room temperature, ensuring a prolonged shelf life and eliminating the need for cold storage.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eUser Convenience\u003c\/strong\u003e: Pre-mixed and pre-dispensed format significantly reduces preparation time and minimizes the risk of pipetting errors.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVersatility\u003c\/strong\u003e: Suitable for a variety of PCR applications, including endpoint PCR and intercalating dye-based assays.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Performance\u003c\/strong\u003e: Incorporates high-quality recombinant HotStart DNA polymerase, ensuring robust and reproducible amplification results.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Instructions:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eStore the beads at ~2-8℃ in an airtight foil pouch containing desiccant. Once the pouch is opened, it should be completely resealed, folded over several times, and secured with a clip. For optimal product longevity, store unopened and resealed pouches in a desiccator.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eComponents of lyophilized PCR Beads:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003edATP, dCTP, dGTP, dTTP\u003c\/li\u003e\n\u003cli\u003e~2.5 units of HotStart DNA polymerase\u003c\/li\u003e\n\u003cli\u003eStabilizers\u003c\/li\u003e\n\u003cli\u003eReaction buffer\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eUsage Instructions:\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cstrong\u003eAdd Primers\u003c\/strong\u003e: Introduce 5'- and 3'-primers (5–25 pmol each) into the reaction tube containing the PCR bead.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAdd Template\u003c\/strong\u003e: Add the DNA template (50 pg for plasmid DNA or 50 ng for genomic DNA) to the tube.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAdd Water\u003c\/strong\u003e: Add DNase-free water to achieve a final reaction volume of 25 μl.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eSee manual for specific details:\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/MASTER-MAX-PCR-Beads_GBS-Manual-Feb-2025.pdf?v=1740059072\" rel=\"noopener\" target=\"_blank\"\u003e\u003cimg height=\"53\" width=\"188\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\"\u003e\u003c\/a\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/TransIntro_EL_Transfection_Reagent_FT201_-_GBS_Manual_-_Jan-2025.pdf?v=1738070232\" rel=\"noopener\" target=\"_blank\"\u003e\u003c\/a\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eThermal Cycling Conditions:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eInitial Denaturation\u003c\/strong\u003e: 95°C for 6 minutes\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCycling\u003c\/strong\u003e: 95°C for 20 seconds, 55°C for 20 seconds, 72°C for 30-60 seconds (25-40 cycles)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFinal Extension\u003c\/strong\u003e: 72°C for 10 minutes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eCatalog Numbers:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003ePCR-80-08\u003c\/strong\u003e: 0.2 ml 8-strip tubes, 48 reactions (6x 8 reactions)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePCR-80-96\u003c\/strong\u003e: 0.2 ml 8-strip tubes, 96 reactions (12x 8 reactions)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eMaterials Not Supplied:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDeionized or Distilled Water\u003c\/strong\u003e: Ensure the water used is nuclease-free and free of contaminating nucleic acids.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDNA Template\u003c\/strong\u003e: High-quality DNA is recommended for optimal results.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTemplate-Specific Primers\u003c\/strong\u003e: Primers should be designed according to the guidelines provided in the product manual.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMineral Oil\u003c\/strong\u003e: If required for the thermal cycler being used.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eFor Research Use Only:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eThese beads are intended solely for research purposes and are not suitable for diagnostic procedures. They should not be used internally or externally in humans or animals. \u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"48 Rxns (8-Strip)","offer_id":40142061207652,"sku":"PCR-80-08","price":240.7,"currency_code":"CAD","in_stock":true},{"title":"96 Rxns (8-Strip)","offer_id":40142061240420,"sku":"PCR-80-96","price":459.64,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/648.jpg?v=1643777496"},{"product_id":"lyse-n-amptm-mouse-genotyping-kit","title":"Lyse n Amp(TM) Mouse Genotyping Kit","description":"\u003cp\u003e20 µL per reactions for 10 mL reactions for the 500 rxn size, and 20 mL reactions for the 1000 rxn size.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eUsing the Lyse n Amp(TM) Mouse Genotyping Kit, there is no need of cumbersome homogenization, crushing, overnight digestion, phenol chloroform extraction, DNA precipitation or column \u003c\/span\u003e\u003cspan\u003epurification operations, which greatly shortens the experimental time. \u003cspan\u003eThe extracted genomic DNA can be used directly as \u003c\/span\u003e\u003cspan\u003etemplate for PCR amplification to evaluate if the mouse contained the target DNA. \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eThis Kit supplies both the lysis reagents and 2 × Taq Plus Master Mix (Dye Plus) (P212), which includes a high-performance Taq Plus DNA Polymerase, dNTP, and optimized \u003c\/span\u003e\u003cspan\u003ebuffer system to ensures high amplification efficiency of target DNA. Because Taq DNA Polymerase has the 3' terminal transferase activity, the dA overhang defines each of the 3' ends of the PCR product. Therefore, the resulting PCR product can be cloned into any T vector, such as those of GeneBio Systems' TA cloning vector (https:\/\/www.genebiosystems.com\/50-ta-cloning-kits). \u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003e\u003cspan\u003eLiterature citing the use of this kit:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e1. Han W, He X, Zhang M, et al. (2015). Establishment of a porcine pancreatic stem cell line using T-RExTM system-inducible Wnt3a expression. Cell Prolif,48(3):301-310. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eIn addition, the same reagents were also used under a different product name to genotype mice:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. Zhou L et al. Decorin promotes proliferation and migration of ORS keratinocytes and maintains hair anagen in mice. Exp Dermatol. 2018 Nov;27(11):1237-1244. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e3. Li H et al., The effects of Nrf2 knockout on regulation of benzene-induced mouse hematotoxicity. Toxicol Appl Pharmacol. 2018 Nov 1;358:56-67.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e4. Su D et al. One-step generation of mice carrying a conditional allele together with an HA-tag insertion for the delta opioid receptor.Sci Rep. 2017 Mar 16;7:44476.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e5. Xiao J et al. \u003c\/span\u003eRole of major and brain-specific Sgce isoforms in the pathogenesis of myoclonus-dystonia syndrome. Neurobiol Dis. 2017 Feb;98:52-65.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e6. Xiao J et al. \u003c\/span\u003eMotor phenotypes and molecular networks associated with germline deficiency of Ciz1. Exp Neurol. 2016 Sep;283(Pt A):110-20.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/GBS_Lyse_n_Amp_Mouse_Genotyping_Kit_-500rxn_and_1000_rxn.pdf?v=1700833740\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"178\" height=\"50\"\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"1000rxns","offer_id":39892628373604,"sku":"PD101-02","price":703.58,"currency_code":"CAD","in_stock":true},{"title":"500 rxns","offer_id":39892599963748,"sku":"PD101-01","price":390.88,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/658.jpg?v=1643777539"},{"product_id":"transdirect-mouse-genotyping-kit","title":"TransDirect Mouse Genotyping Kit","description":"\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eAppplication\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e• Direct PCR amplification from crude lysate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Suitable for PCR-based rapid mouse genotyping.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Easy-to-use and suitable for high-throughput applications.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Suitable for multiplex PCR with up to 5 pairs of primers.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Directly load products without needing to add gel loading dye. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eat -20°C for two years\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eKit Contents\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eComponent\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eAD501-01 (100 rxns)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eAD501-02 (500 rxns)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eAD1 Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e12 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e60 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eAD2 Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e3 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e15 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e2×TransDirect® Mouse Genotyping SuperMix (+dye)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1 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With more than 13 high-impact factor journals citing the use of this product and known for its robust amplification for up to 6 kb of PCR products in size and resistance to impurities, this reagent should work for most of your PCR and amplification applications*.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eGB-Amp™ 2 x PCR Master mix (without loading dye) \u003c\/span\u003eis provided without gel loading dyes. If you need a robust 2x PCR Master Mix with an insert gel loading dye already added, please consider our BLU-Taq™ 2× Taq Master Mix.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eTaq\u003c\/em\u003e DNA Polymerase included in this master mix is a thermostable DNA polymerase that possesses a 5 '→3 ' polymerase activity and a 5 ' flap endonuclease activity. The PCR products contain A at the 3'-end and thus can be directly cloned into T-Vectors of TA cloning kits.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eGB-Amp™ 2 x PCR Master mix (without loading dye)\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable width=\"697\" style=\"height: 207px;\" data-mce-style=\"height: 207px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eCat#\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eSize \u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003ePrice\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eList Price ($)\/ml\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"4\"\u003e\n\u003cp\u003e\u003cspan\u003eGB-Amp™ 2 x PCR Master mix (without loading dye)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eP4001\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e26\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e26\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eP4002\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e5 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mL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e390\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e7.8\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/GB-Amp_2_x_PCR_Master_mix_without_loading_dye.pdf?v=1700752154\" data-mce-href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/GB-Amp_2_x_PCR_Master_mix_without_loading_dye.pdf?v=1700752154\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cimg height=\"46\" width=\"164\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\" data-mce-fragment=\"1\" data-mce-selected=\"1\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\"\u003e\u003c\/span\u003e \u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"1 ml","offer_id":39771525185636,"sku":"P4001","price":33.02,"currency_code":"CAD","in_stock":true},{"title":"5 ml","offer_id":39771525087332,"sku":"P4002","price":115.21,"currency_code":"CAD","in_stock":true},{"title":"15 ml","offer_id":39771525120100,"sku":"P4003","price":296.35,"currency_code":"CAD","in_stock":true},{"title":"50 ml","offer_id":39771525152868,"sku":"P4004","price":858.21,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/p4001_image.jpg?v=1742213704"},{"product_id":"gb-script-iii-reverse-transcriptase","title":"GB-Script™  III Reverse 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The 5× gDNA wiper Mix in the kit can quickly remove genomic DNA contamination at 42℃ for 2 min, which makes the results more reliable and simplifies qPCR primer design process without the need to design primers across introns. The kit contains two separated primers: the reverse transcription primers Oligo (dT) 20 VN and Random hexamers, allowing users to choose primers flexibly for subsequent experiments as needed. The kit can synthesize full-length cDNA (up to 20 kb) for downstream experiments such as cloning, and can also synthesize a highly uniform cDNA for qPCR quantification.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/R312-V21.1HiScript_III_1st_Strand_cDNA-GBS.pdf?v=1700838009\" data-mce-href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/R312-V21.1HiScript_III_1st_Strand_cDNA-GBS.pdf?v=1700838009\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"228\" height=\"64\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 273px;\" width=\"720\" data-mce-style=\"height: 273px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eR312-01  50\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003erxn \u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003e(20\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eμl\/rxn)\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eR312-02 \u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003e100\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003erxn 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μl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e10x \u003cspan\u003eRT\u003c\/span\u003eMix\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e100 μl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e200 μl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eGB-Script™ \u003c\/span\u003e III EnzymeMix\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e100 μl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e200 μl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eOligo (dT)\u003c\/span\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eVN\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e50 μl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e100 μl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eRandomHexamers\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e50 μl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e100 μl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Gene Bio Systems","offers":[{"title":"50 reactions","offer_id":39771525283940,"sku":"R312-01","price":440.16,"currency_code":"CAD","in_stock":true},{"title":"100 reactions","offer_id":39771525316708,"sku":"R312-02","price":827.68,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_1f7fc20e-7292-4a41-85e3-e7287408449a.jpg?v=1706709612"},{"product_id":"2-phanta-max-master-mix","title":"2X Phanta Max Master Mix","description":"\u003cp\u003e \u003c\/p\u003e\n\u003ch2 data-start=\"111\" data-end=\"145\"\u003e2× Phanta Max Master Mix\u003c\/h2\u003e\n\u003cp data-start=\"146\" data-end=\"191\" class=\"\"\u003e\u003cstrong data-start=\"146\" data-end=\"191\"\u003eUltra-High Fidelity. Supreme Performance.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-start=\"193\" data-end=\"607\" class=\"\"\u003e\u003cstrong data-start=\"193\" data-end=\"221\"\u003e2× Phanta Max Master Mix\u003c\/strong\u003e is a high-performance PCR solution featuring \u003cstrong data-start=\"267\" data-end=\"311\"\u003ePhanta Max Super-Fidelity DNA Polymerase\u003c\/strong\u003e, optimized for long-fragment amplification, superior specificity, and exceptionally low error rates. Thanks to its unique \u003cstrong data-start=\"434\" data-end=\"454\"\u003eextension factor\u003c\/strong\u003e, \u003cstrong data-start=\"456\" data-end=\"489\"\u003especificity-promoting factors\u003c\/strong\u003e, and \u003cstrong data-start=\"495\" data-end=\"524\"\u003eplateau-inhibiting factor\u003c\/strong\u003e, Phanta Max delivers unmatched fidelity and yield, even for challenging templates.\u003c\/p\u003e\n\u003cp data-start=\"609\" data-end=\"927\" class=\"\"\u003eCapable of amplifying fragments up to \u003cstrong data-start=\"647\" data-end=\"664\"\u003e40 kb (λ DNA)\u003c\/strong\u003e, \u003cstrong data-start=\"666\" data-end=\"689\"\u003e20 kb (genomic DNA)\u003c\/strong\u003e, and \u003cstrong data-start=\"695\" data-end=\"711\"\u003e10 kb (cDNA)\u003c\/strong\u003e, it’s an excellent choice for demanding applications. With an error rate \u003cstrong data-start=\"785\" data-end=\"807\"\u003e53x lower than Taq\u003c\/strong\u003e and \u003cstrong data-start=\"812\" data-end=\"833\"\u003e6x lower than Pfu\u003c\/strong\u003e, Phanta Max ensures high accuracy for cloning, sequencing, and other downstream applications.\u003c\/p\u003e\n\u003ch2 data-start=\"934\" data-end=\"956\"\u003eKey Features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli data-start=\"957\" data-end=\"1666\"\u003e\n\u003cstrong data-start=\"959\" data-end=\"982\"\u003eUltra-High Fidelity\u003c\/strong\u003e – Ideal for accurate amplification and cloning\u003cbr data-start=\"1029\" data-end=\"1032\"\u003e\u003cstrong data-start=\"1034\" data-end=\"1069\"\u003e\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"957\" data-end=\"1666\"\u003e\n\u003cstrong data-start=\"1034\" data-end=\"1069\"\u003eExceptional Amplification Range\u003c\/strong\u003e – 40 kb λ DNA, 20 kb genomic DNA, 10 kb cDNA\u003cbr data-start=\"1114\" data-end=\"1117\"\u003e\u003cstrong data-start=\"1119\" data-end=\"1150\"\u003e\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"957\" data-end=\"1666\"\u003e\n\u003cstrong data-start=\"1119\" data-end=\"1150\"\u003eStrong Inhibitor Resistance\u003c\/strong\u003e – Direct PCR from bacteria, fungi, plant tissue, animal tissue, and even whole blood\u003cbr data-start=\"1235\" data-end=\"1238\"\u003e\u003cstrong data-start=\"1240\" data-end=\"1264\"\u003e\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"957\" data-end=\"1666\"\u003e\n\u003cstrong data-start=\"1240\" data-end=\"1264\"\u003eHot-Start Technology\u003c\/strong\u003e – Dual monoclonal antibodies inhibit polymerase and exonuclease activity at room temperature\u003cbr data-start=\"1357\" data-end=\"1360\"\u003e\u003cstrong data-start=\"1362\" data-end=\"1384\"\u003e\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"957\" data-end=\"1666\"\u003e\n\u003cstrong data-start=\"1362\" data-end=\"1384\"\u003eBlunt-End Products\u003c\/strong\u003e – Perfect for cloning with \u003cstrong data-start=\"1412\" data-end=\"1452\"\u003eClonExpress II One Step Cloning Kits\u003c\/strong\u003e (Cat. \u003ca href=\"https:\/\/www.genebiosystems.com\/products\/clonexpress-ii-one-step-cloning-kit\" rel=\"noopener\" target=\"_blank\"\u003e#C112\u003c\/a\u003e, #\u003ca href=\"https:\/\/www.genebiosystems.com\/products\/clonexpress-multis-one-step-cloning-kit\" rel=\"noopener\" target=\"_blank\"\u003eC113\u003c\/a\u003e)\u003cbr data-start=\"1472\" data-end=\"1475\"\u003e\u003cstrong data-start=\"1477\" data-end=\"1506\"\u003e\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"957\" data-end=\"1666\"\u003e\n\u003cstrong data-start=\"1477\" data-end=\"1506\"\u003eConvenient \u0026amp; Reproducible\u003c\/strong\u003e – Just add primers and template; optimized for consistent performance\u003cbr data-start=\"1576\" data-end=\"1579\"\u003e\u003cstrong data-start=\"1581\" data-end=\"1603\"\u003e\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"957\" data-end=\"1666\"\u003e\n\u003cstrong data-start=\"1581\" data-end=\"1603\"\u003eFreeze-Thaw Stable\u003c\/strong\u003e – Protective formulation resists multiple freeze-thaw cycles\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 data-start=\"1673\" data-end=\"1702\"\u003eIncluded in the Mix\u003c\/h2\u003e\n\u003cul data-start=\"1703\" data-end=\"1787\"\u003e\n\u003cli data-start=\"1703\" data-end=\"1747\" class=\"\"\u003e\n\u003cp data-start=\"1705\" data-end=\"1747\" class=\"\"\u003ePhanta Max Super-Fidelity DNA Polymerase\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1748\" data-end=\"1757\" class=\"\"\u003e\n\u003cp data-start=\"1750\" data-end=\"1757\" class=\"\"\u003edNTPs\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1748\" data-end=\"1757\" class=\"\"\u003e\n\u003cp data-start=\"1750\" data-end=\"1757\" class=\"\"\u003eOptimized reaction buffer\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 data-start=\"1794\" data-end=\"1811\"\u003eStorage\u003c\/h2\u003e\n\u003cp data-start=\"1812\" data-end=\"1872\" class=\"\"\u003e❄ \u003cstrong data-start=\"1814\" data-end=\"1832\"\u003eStore at –20°C\u003c\/strong\u003e for maximum stability and performance\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"1mL","offer_id":39771527872612,"sku":"P505-01","price":139.71,"currency_code":"CAD","in_stock":true},{"title":"5mL","offer_id":39771527807076,"sku":"P505-02","price":508.03,"currency_code":"CAD","in_stock":true},{"title":"15mL","offer_id":39771527839844,"sku":"P505-03","price":1270.08,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/741.jpg?v=1643777949"},{"product_id":"5min-ta-blunt-zero-cloning-kit","title":"5min TA-Blunt-Zero Cloning Kit","description":"\u003cp\u003e\u003cspan\u003e\u003cspan\u003eIn combination with an optimal buffer, the second generation of Topoisomerase, this product using a vector containing the suicide \u003c\/span\u003e\u003c\/span\u003egene ccdB, when the insert is successfully ligated to the vector, the correct expression of ccdB is destroyed, and the host cell can grow normally, otherwise the host cell cannot grow normally, thereby achieving “zero” background. Containing a blunt end factor, 5min TA\/Blunt-Zero Cloning Kit is compatible with both TA clones and blunt clones.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eFeatures-\u003c\/p\u003e\n\u003cul class=\"list-paddingleft-2\"\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eCompatible: \u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003eSuitable for sticky ends,\u003cstrong\u003e blunt ends\u003c\/strong\u003e, \u003cstrong\u003eTA\u003c\/strong\u003e , linker or adapter ligation\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFast: \u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003eCloning of either sticky- or blunt-end DNA in only \u003cstrong\u003e5 minutes\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eEfficient: \u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003eHigh efficiency cloning and positive rate \u003cstrong\u003e\u0026gt;95%\u003c\/strong\u003e. Get \u003cstrong\u003emany colonies\u003c\/strong\u003e and pick one colony, you have it. \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eIf you and your colleagues use both \u003cstrong\u003eTaq\u003c\/strong\u003e, Pfu or another \u003cstrong\u003eproof-reading polymerase, such as PaCeR, \u003c\/strong\u003ein PCR, you do not need to worry about if you need to stock both the TA cloning kit and the blunt end cloning kit, or worry about using the wrong kit. Just keep this kit in the lab and have peace of mind in using this cloning kit for all your PCR cloning Needs.\u003c\/p\u003e\n\u003cp\u003eAvailabl\u003cstrong\u003ee in kit including ready to use competent cells with 20 reaction (10 x 100uL\u003c\/strong\u003e\u003cspan style=\"text-decoration: underline;\"\u003e\u003cstrong\u003e \u003ca href=\"https:\/\/www.genebiosystems.com\/products\/trans5alpha-chemically-competent-cell?variant=39766552576100\"\u003eCloning competent cells\u003c\/a\u003e. \u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/C601-5minTM_TA_Blunt-Zero_Cloning_Kit-GBS.pdf?v=1709224030\"\u003e\u003cspan style=\"text-decoration: underline;\"\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\" width=\"185\" height=\"52\"\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"10 rxns-without competent cells","offer_id":41805273464932,"sku":"C601-00","price":192.33,"currency_code":"CAD","in_stock":true},{"title":"25 rxns-without competent cells","offer_id":39886927331428,"sku":"C601-01","price":384.65,"currency_code":"CAD","in_stock":true},{"title":"25 rxns-with competent cells","offer_id":40404410368100,"sku":"C601-01CC","price":514.08,"currency_code":"CAD","in_stock":true},{"title":"50 rxns - without competent cells","offer_id":44036020273252,"sku":"C601-02","price":588.23,"currency_code":"CAD","in_stock":true},{"title":"50 rxns - with competent cells","offer_id":44036038623332,"sku":"C601-02CC","price":871.71,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/5minTABluntZeroCloningKit.webp?v=1669309625"},{"product_id":"gb-amp-hotstart-taq-2x-master-mix-with-loading-dye","title":"GB-Amp™ HotStart Taq 2X Master Mix (with Loading Dye)","description":"\u003cdiv\u003eUsed for end-point PCR, this product is available in a version containing electrophoresis buffer and dye. It can be directly electrophoresed after the reaction. The 3' end of the PCR product, A base, can be directly cloned into the T vectors (e.g., our TA-cloning kits: \u003ca href=\"https:\/\/www.genebiosystems.com\/50-ta-cloning-kits\"\u003ehttps:\/\/www.genebiosystems.com\/50-ta-cloning-kits\u003c\/a\u003e)\u003c\/div\u003e\n\u003cp\u003eWhen using this product for qPCR reactions, reliable standard curve can be obtained in a wide range and accurate qualification of target genes can be achieved with high repeatability and reliability.\u003c\/p\u003e\n\u003cp\u003eThis master mix contains loading dye for gel, so that after PCR, you can just load the PCR product to gel, without having to add gel loading dye. \u003c\/p\u003e\n\u003ch2\u003e\u003c\/h2\u003e\n\u003ch2\u003e\u003c\/h2\u003e","brand":"Gene Bio Systems","offers":[{"title":"1 ml","offer_id":39771529543780,"sku":"P2041b","price":36.84,"currency_code":"CAD","in_stock":true},{"title":"5 ml","offer_id":39771529478244,"sku":"P2042b","price":160.03,"currency_code":"CAD","in_stock":true},{"title":"10 ml","offer_id":39771529511012,"sku":"P2043b","price":304.82,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_4e53197c-d768-4bbe-a096-93fd72fb6837.jpg?v=1694781850"},{"product_id":"hiscript-r-ii-one-step-qrt-pcr-probe-kit","title":"HiScript® II One Step qRT-PCR Probe Kit","description":"\u003cp\u003e\u003cimg alt=\"\" src=\"http:\/\/www.vazymebiotech.com\/imageRepository\/990234f9-2584-4498-8fa9-9ffaea05b8cc.png\"\u003e\u003cstrong\u003eSize:\u003c\/strong\u003e 100 Rxn (50 ul\/Rxn)\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eIt is critically challenging for detection of low-copy templates and weak-positive samples in clinical diagnosis. HiScript\u003csup\u003e®\u003c\/sup\u003e II One Step qRT-PCR Probe Kit contains the optimal proportion of HiScript\u003csup\u003e®\u003c\/sup\u003e II Reverse Transcriptase and Champagne Taq\u003csup\u003eTM\u003c\/sup\u003e DNA Polymerase and optimized buffer system. No matter for total RNA, in-vitro-transcribed RNA or virus RNA, HiScript\u003csup\u003e®\u003c\/sup\u003e II One Step qRT-PCR Probe Kit shows extremely high sensitivity and stable amplification performance. Multi amplification can also achieve without much optimization.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39771529609316,"sku":"Q222","price":518.92,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_6d0d3247-1516-4793-ad75-7b0cac73f5c4.jpg?v=1705589195"},{"product_id":"hiscript-ii-u-one-step-qrt-pcr-probe-kit","title":"HiScript® II U One Step qRT-PCR Probe Kit","description":"\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cimg alt=\"\" src=\"http:\/\/www.vazymebiotech.com\/imageRepository\/231fe888-713d-4b26-8d95-8864c40f60f5.png\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eHiScript II U+ One Step qRT-PCR Probe Kit  is specially designed for qPCRs that directly use RNA (i.e. virus RNA) as templates. Using\u003c\/span\u003e\u003c\/span\u003e \u003cspan\u003e\u003cspan\u003egene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube, significantly reducing pipetting procedures and the risk of\u003c\/span\u003e\u003c\/span\u003e \u003cspan\u003e\u003cspan\u003econtamination. This kit contains a dUTP\/ UDG system that can prevent the contamination of PCR product. The HiScript II Reverse Trancriptase and hot-start\u003c\/span\u003e\u003c\/span\u003e \u003cspan\u003e\u003cspan\u003eChampagne Taq DNA Polymerase in this kit enable high-sensitive total RNA detection (as little as 0.1 pg or \u0026lt; 10 copies). The HiScript II U+ One Step qRT-PCR\u003c\/span\u003e\u003c\/span\u003e \u003cspan\u003e\u003cspan\u003eProbe Kit is provided in master mix. The 2× One Step U+ Mix contains an optimized buffer and dNTP\/dUTP mix, and is suitable for high-sensitive detection\u003c\/span\u003e\u003c\/span\u003e \u003cspan\u003e\u003cspan\u003esystems based on fluorescence labelled probes (i.e. TaqMan®).\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cimg alt=\"\" src=\"http:\/\/www.vazymebiotech.com\/imageRepository\/5b10290c-416e-4665-9583-26cc1da5b588.png\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ctable border=\"1\" cellpadding=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cstrong\u003e\u003cspan\u003e\u003cspan\u003eCATALOG\u003c\/span\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cstrong\u003e\u003cspan\u003e\u003cspan\u003eSIZE\u003c\/span\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eQ222-CN-00\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e100 tests\/kit\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eQ222-CN\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e5,000 tests\/kit\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cimg alt=\"\" src=\"http:\/\/www.vazymebiotech.com\/imageRepository\/74d90b1a-3a12-4eed-bc96-58a76dfaf7b6.png\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eAll components should be stored at -20\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003cspan\u003e℃\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39771529642084,"sku":"Q223","price":518.92,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_19fdf326-0fdb-4b84-aecd-0e91ed66c885.jpg?v=1705589123"},{"product_id":"hiscript-iii-rt-supermix-for-qpcr-gdna-wiper","title":"HiScript® III RT SuperMix for qPCR (+gDNA wiper)","description":"\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eThe 4 × gDNA wiper Mix in the kit can completely remove the remaining genomic DNA in the RNA template, ensure that the subsequent quantitative results are more reliable, and simplify the design of qPCR primers without the need to design primers across introns; 5 × HiScript III qRT SuperMix contains All components required for reverse transcription reaction can be reacted quickly by adding template RNA and water, and at the same time, the gDNA wiper function is terminated to ensure the integrity of cDNA. The reverse transcription product is compatible with dye method and probe method qPCR, which can perform high-performance gene expression analysis. \u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eEasy and quick operation: 5 × HiScript III qRT SuperMix contains all the components required for reverse transcription reaction, only need to add template RNA, the reverse transcription reaction can be completed within 20 minutes;\u003cbr\u003eExtensive template compatibility: Compatible with RNA templates of different species and poor integrity;\u003cbr\u003eSuper impurity tolerance: It has super tolerance to common reverse transcription impurities such as ethanol, isopropanol, water balance phenol, guanidine isothiocyanate, humic acid, etc.;\u003cbr\u003eExcellent reverse transcription efficiency: Compared with common commercial reverse transcription products, HiScript ® III RT SuperMix for qPCR (+gDNA wiper) reverses the smallest cDNA quantitative C T value and has excellent reverse transcription efficiency.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/R323_GBS-Manual_Jan-2025.pdf?v=1738600412\" rel=\"noopener\" target=\"_blank\"\u003e\u003cimg data-mce-fragment=\"1\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"228\" height=\"64\"\u003e\u003c\/a\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/R323-V22.1-GBS.pdf?v=1700840382\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eStore at -30~-15°C\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"100 rxns","offer_id":40170552492132,"sku":"R323-01","price":712.45,"currency_code":"CAD","in_stock":true},{"title":"200 rxns","offer_id":42760224702564,"sku":"R323-02","price":1282.42,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_c65bb98d-e643-4f52-a7b0-d365895a3571.jpg?v=1706708940"},{"product_id":"hiscript-ii-one-step-rt-pcr-kit","title":"HiScript® II One Step RT-PCR Kit","description":"\u003cp\u003e\u003cstrong\u003eSize: 125 rxn (20 uL\/reaction)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eValidation Results:\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eA. Taking total RNA of Hela cells as template, the GAPDH fragment of 555 bp was amplified using HiScript II One Step RT-PCR Kit (#P611). The detection sensitivity can reach 0.1 pg.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eB. Taking PPRSV RNA as template, a fragment of 660 bp was amplified using HiScript II One Step RT-PCR Kit (#P611). The detection sensitivity can reach 0.2 TCLD50.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eC. Taking 1 μg of total RNA from Hela cells as template, fragments with different lengths were amplified using HiScript II One Step RT-PCR Kit (#P611). The UTRN fragments as long as 12.1 kb were successfully amplified.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan lang=\"en-us\"\u003eThe HiScript II One Step RT-PCR Kit is specially designed for RNA detection (such as RNA virus). With the HiScript II One Step RT-PCR Kit and gene-specific primers (GSP), both reverse transcription and PCR amplification are performed in the same tube, with no additional pipetting procedures, which improves detection through-put and minimizes potential contamination. This kit contains HiScript II Reverse Transcriptase, Champagne Taq plus hot-start DNA Polymerase, and an optimized buffer, which enables high-sensitive total RNA detection and long-fragment amplification (as long as 10 kb).\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eP611-01 50 rxn (50 ul\/rxn)\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eRNase free ddH\u003csub\u003e2\u003c\/sub\u003eO\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e1 ml\u003c\/span\u003e×\u003cspan\u003e2\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e ×\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eOne Step Mix\u003csup\u003ea\u003c\/sup\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e1 ml\u003c\/span\u003e×\u003cspan\u003e2\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eOne Step Enzyme Mix\u003csup\u003eb\u003c\/sup\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e125 μl\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e ×\u003c\/span\u003e\u003cspan\u003e Loading buffer\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e1.25 ml\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003ea. Contains dNTPs.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eb. Contains RNase inhibitor, HiScript II Reverse Transcriptase, and Champagne Taq plus Polymerase.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eStorage: \u003cspan\u003eAll components should be stored at -20\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/P611_HiScript_II_One_Step_RT-PCR-GBS.pdf?v=1700835445\"\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"149\" height=\"42\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39771529969764,"sku":"P611","price":325.38,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_f6729fcc-282f-4157-b415-8aa8879ef785.jpg?v=1706711014"},{"product_id":"hiscript-ii-one-step-rt-pcr-supermix-dye-plus","title":"HiScript® II One Step RT-PCR SuperMix (Dye Plus)","description":"\u003cp\u003e\u003cstrong\u003eSize: 50 rxn (50 ul\/rxn) \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe HiScript II One Step RT-PCR SuperMix (Dye Plus) is specially designed for RNA detection (such as RNA virus). With the HiScript II One Step RT-PCR SuperMix and gene-specific primers (GSP), both reverse transcription and PCR amplification are performed in the same tube, with no additional pipetting procedures, which improves detection through-put and minimizes potential contamination. This SuperMix contains HiScript II Reverse Transcriptase, RNase inhibitor, and Champagne Taq plus hot-start DNA Polymerase, which enables high-sensitive total RNA detection (as little as 1 pg) and long-fragment amplification (as long as 10 kb). The 2\u003c\/span\u003e\u003cspan\u003e× \u003c\/span\u003e\u003cspan\u003eOne Step Mix (Dye Plus) contains an opimized buffer, dNTPs, and Loading Dye.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"http:\/\/www.vazymebiotech.com\/imageRepository\/5b10290c-416e-4665-9583-26cc1da5b588.png\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"0\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003ecomponents\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eP612-01 50 rxn (50 ul\/rxn)\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eRNase free ddH\u003csub\u003e2\u003c\/sub\u003eO\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e1 ml×2\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp align=\"left\"\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003eOne Step Mix\u003csup\u003ea\u003c\/sup\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e625 μl×2\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eOne Step Enzyme Mix\u003csup\u003eb\u003c\/sup\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e125 μl\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp align=\"left\"\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003ea. Contains dNTPs and Loading Dye.\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eb. Contains RNase inhibitor, HiScript II Reverse Transcriptase, and Champagne Taq plus Polymerase.\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStore at -20 C. \u003c\/span\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39771530002532,"sku":"P612","price":325.38,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_1f8631f3-ebf4-4168-bdc4-70fac6e5aa2f.jpg?v=1706710983"},{"product_id":"one-step-probe-rt-qpcr-kit","title":"One-step Probe RT-qPCR Kit","description":"\u003cp\u003e\u003cstrong\u003eSize:\u003c\/strong\u003e 100 rxns\u003c\/p\u003e\n\u003cp\u003eHeat-labile UDG can rapidly degrade the pollutants containing U at room temperature. \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eWhen reverse transcription is performed at 55°C, heat-labile UDG will be inactivated rapidly without affecting the efficiency and sensitivity of RT-qPCR.\u003c\/span\u003e\u003cspan\u003e Integrating the superior performance of Reverse Transcriptase and hot-start\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eTaq DNA Polymerase, with the optimized buffer system, the detection sensitivity of \u003c\/span\u003e\u003cspan\u003eOne-step\u003c\/span\u003e\u003cspan\u003e Probe\u003c\/span\u003e\u003cspan\u003e RT-qPCR Kit\u003c\/span\u003e\u003cspan\u003e can reach 0.1 pg total RNA or \u0026lt;10 copies of RNA template.\u003c\/span\u003e\u003cspan\u003e The kit is available as a convenient Master Mix. 2 × One-step U\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e+\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e Mix contains an optimized buffer system and dNTP\/dUTP Mix, which is suitable for high-specificity detection systems with fluorescent labeled probes such as TaqMan\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e®\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e. This product is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-Rad, etc.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e\u003cspan\u003eApplications\u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003cspan\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Probe gene expression analysis\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Probe Low-copy gene detection\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Probe microarray validation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Probe gene knockdown validation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003cspan\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• For One-step RT-qPCR operation  \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• This kit is suitable for fluorescence quantification by probe method\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• This kit is compatible with many real-time systems\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Hot-start technology brings high specificity and reproducible amplification\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• dUTP\/UDG system, effectively prevent PCR product contamination\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e• Multiplex detection\u003c\/p\u003e\n\u003cp\u003e• Supports high-performance PCR detection\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39771531116644,"sku":"v5001","price":361.67,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_eade14e5-44d6-4c53-a643-4cab79e07288.jpg?v=1705589082"},{"product_id":"blood-direct-pcr-kit","title":"Blood Direct PCR Kit","description":"\u003cp\u003e The kit contains Phanta Blood Super-Fidelity DNA Polymerase, which is engineered on the basis of Phanta Super-Fidelity DNA Polymerase. The enzyme is highly resistant to PCR inhibitors in whole blood samples, and can amplify whole blood concentrations up to 40%. Coupled with carefully optimized 2 × Phanta Blood Buffer V2, Blood Direct PCR Kit V2 can efficiently amplify genome fragments up to 10 kb from whole blood samples. Phanta Blood Super-Fidelity DNA Polymerase amplification products are blunt-ended, suitable for ClonExpress® rapid cloning kit (C112\/C113). The kit is equipped with a Positive control primer mix compatible with mammals and many other vertebrates, which can be used for positive control reactions. \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eBased on the modified Phanta® Blood Super-Fidelity DNA Polymerase, it has extremely strong amplification efficiency and PCR inhibitor tolerance;\u003cbr\u003eSuitable for various blood samples, compatible with conventional anticoagulants;\u003cbr\u003eCan tolerate up to 40% of whole blood template;\u003cbr\u003eCan efficiently amplify genome fragments up to 7.5 kb from whole blood\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eStore at -20°C\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"50 rxns","offer_id":39771531542628,"sku":"PD103-01","price":240.71,"currency_code":"CAD","in_stock":true},{"title":"200 rxns","offer_id":39771531509860,"sku":"PD103-02","price":881.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/noimagedefaultimage-jpeg_3d0c32ce-db0e-4880-ad0d-5842a0b273d0.jpg?v=1647567035"},{"product_id":"murine-rnase-inhibitor","title":"Murine RNase Inhibitor","description":"\u003ch2 data-start=\"311\" data-end=\"368\"\u003eReliable RNase Protection for Sensitive RNA Workflows\u003c\/h2\u003e\n\u003cp data-start=\"370\" data-end=\"665\" class=\"\"\u003eMurine RNase Inhibitor is a high-performance, recombinant enzyme derived from mouse and expressed in \u003cem data-start=\"471\" data-end=\"480\"\u003eE. coli\u003c\/em\u003e with high purity and solubility. It delivers powerful inhibition against a wide range of RNases (RNase A, B, and C), and is designed for optimal performance in RT-PCR and qPCR systems.\u003c\/p\u003e\n\u003ch2 data-start=\"672\" data-end=\"695\"\u003eKey Features\u003c\/h2\u003e\n\u003cul data-start=\"697\" data-end=\"1335\"\u003e\n\u003cli data-start=\"697\" data-end=\"870\" class=\"\"\u003e\n\u003cp data-start=\"699\" data-end=\"870\" class=\"\"\u003e\u003cstrong data-start=\"699\" data-end=\"729\"\u003eOxidation-Resistant Design\u003c\/strong\u003e\u003cbr data-start=\"729\" data-end=\"732\"\u003eLacks two cysteines found in human RNase inhibitors, offering \u003cstrong data-start=\"796\" data-end=\"830\"\u003esuperior antioxidant stability\u003c\/strong\u003e—ideal for oxidation-prone environments.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"872\" data-end=\"1009\" class=\"\"\u003e\n\u003cp data-start=\"874\" data-end=\"1009\" class=\"\"\u003e\u003cstrong data-start=\"874\" data-end=\"909\"\u003eDTT-Sensitive System Compatible\u003c\/strong\u003e\u003cbr data-start=\"909\" data-end=\"912\"\u003eExcellent for \u003cstrong data-start=\"928\" data-end=\"948\"\u003eqPCR and RT-qPCR\u003c\/strong\u003e workflows where high DTT concentrations can disrupt results.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1011\" data-end=\"1159\" class=\"\"\u003e\n\u003cp data-start=\"1013\" data-end=\"1159\" class=\"\"\u003e\u003cstrong data-start=\"1013\" data-end=\"1040\"\u003eUltra-Pure \u0026amp; RNase-Free\u003c\/strong\u003e\u003cbr data-start=\"1040\" data-end=\"1043\"\u003eExpressed in \u003cem data-start=\"1058\" data-end=\"1067\"\u003eE. coli\u003c\/em\u003e for \u003cstrong data-start=\"1072\" data-end=\"1090\"\u003emaximum purity\u003c\/strong\u003e and \u003cstrong data-start=\"1095\" data-end=\"1115\"\u003eno RNase residue\u003c\/strong\u003e, ensuring consistent, high-quality results.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1161\" data-end=\"1335\" class=\"\"\u003e\n\u003cp data-start=\"1163\" data-end=\"1213\" class=\"\"\u003e\u003cstrong data-start=\"1163\" data-end=\"1186\"\u003eBroad Compatibility\u003c\/strong\u003e\u003cbr data-start=\"1186\" data-end=\"1189\"\u003eWorks seamlessly with:\u003c\/p\u003e\n\u003cul data-start=\"1216\" data-end=\"1335\"\u003e\n\u003cli data-start=\"1216\" data-end=\"1254\" class=\"\"\u003e\n\u003cp data-start=\"1218\" data-end=\"1254\" class=\"\"\u003eHiScript® II Reverse Transcriptase\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1257\" data-end=\"1292\" class=\"\"\u003e\n\u003cp data-start=\"1259\" data-end=\"1292\" class=\"\"\u003eHiScript® Reverse Transcriptase\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1295\" data-end=\"1315\" class=\"\"\u003e\n\u003cp data-start=\"1297\" data-end=\"1315\" class=\"\"\u003eM-MLV (RNase H–)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1318\" data-end=\"1335\" class=\"\"\u003e\n\u003cp data-start=\"1320\" data-end=\"1335\" class=\"\"\u003eDNA Polymerases\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 data-start=\"1342\" data-end=\"1365\"\u003eApplication\u003c\/h2\u003e\n\u003cul data-start=\"1367\" data-end=\"1462\"\u003e\n\u003cli data-start=\"1367\" data-end=\"1387\" class=\"\"\u003e\n\u003cp data-start=\"1369\" data-end=\"1387\" class=\"\"\u003eRT-PCR \u0026amp; RT-qPCR\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1388\" data-end=\"1424\" class=\"\"\u003e\n\u003cp data-start=\"1390\" data-end=\"1424\" class=\"\"\u003eRNA Protection in cDNA Synthesis\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1425\" data-end=\"1462\" class=\"\"\u003e\n\u003cp data-start=\"1427\" data-end=\"1462\" class=\"\"\u003eSensitive RNA Workflow Optimization\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 data-start=\"1469\" data-end=\"1517\"\u003eWhy Choose Our Murine RNase Inhibitor?\u003c\/h2\u003e\n\u003cul data-start=\"1519\" data-end=\"1750\"\u003e\n\u003cli data-start=\"1519\" data-end=\"1566\" class=\"\"\u003e\n\u003cp data-start=\"1521\" data-end=\"1566\" class=\"\"\u003eEnhanced performance under oxidative stress\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1567\" data-end=\"1622\" class=\"\"\u003e\n\u003cp data-start=\"1569\" data-end=\"1622\" class=\"\"\u003eHigher stability compared to human RNase inhibitors\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1623\" data-end=\"1698\" class=\"\"\u003e\n\u003cp data-start=\"1625\" data-end=\"1698\" class=\"\"\u003eExtensively tested for compatibility with reverse transcription systems\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1699\" data-end=\"1750\" class=\"\"\u003e\n\u003cp data-start=\"1701\" data-end=\"1750\" class=\"\"\u003eEasy integration into your existing lab protocols\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eSee manual for more details on R301:\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/R301_Manual_GBS-April_2025.pdf?v=1745336637\" target=\"_blank\" rel=\"noopener\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\" width=\"188\" height=\"53\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eStore at -20℃\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"2000 U","offer_id":39771531608164,"sku":"R301-01","price":83.46,"currency_code":"CAD","in_stock":true},{"title":"10,000 U","offer_id":39771531640932,"sku":"R301-02","price":383.44,"currency_code":"CAD","in_stock":true},{"title":"20,000 U","offer_id":39771531673700,"sku":"R301-03","price":664.07,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/Murine.jpg?v=1702052280"},{"product_id":"multiplex-pcr-kit","title":"Multiplex PCR Kit","description":"\u003cdiv data-node=\"619a7d46d5fdb\" class=\"fl-row fl-row-fixed-width fl-row-bg-none fl-node-619a7d46d5fdb\"\u003e\n\u003cdiv class=\"fl-row-content-wrap\"\u003e\n\u003cdiv class=\"fl-row-content fl-row-fixed-width fl-node-content\"\u003e\n\u003cdiv data-node=\"619a7d46d601f\" class=\"fl-col-group fl-node-619a7d46d601f\"\u003e\n\u003cdiv data-node=\"619a7d46d6062\" class=\"fl-col fl-node-619a7d46d6062\"\u003e\n\u003cdiv class=\"fl-col-content fl-node-content\"\u003e\n\u003cdiv data-animation-delay=\"0.0\" data-node=\"619a7d46d60a1\" class=\"fl-module fl-module-heading fl-node-619a7d46d60a1 pro_tit\"\u003e\n\u003cdiv class=\"fl-module-content fl-node-content\"\u003e\n\u003ch3 class=\"fl-heading\"\u003e\u003cspan class=\"fl-heading-text\"\u003eStorage Conditions\u003c\/span\u003e\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-node=\"619a7d46d60e2\" class=\"fl-col-group fl-node-619a7d46d60e2\"\u003e\n\u003cdiv data-node=\"619a7d46d6124\" class=\"fl-col fl-node-619a7d46d6124\"\u003e\n\u003cdiv class=\"fl-col-content fl-node-content\"\u003e\n\u003cdiv data-animation-delay=\"0.0\" data-node=\"619a7d46d6165\" class=\"fl-module fl-module-rich-text fl-node-619a7d46d6165\"\u003e\n\u003cdiv class=\"fl-module-content fl-node-content\"\u003e\n\u003cdiv class=\"fl-rich-text\"\u003e\n\u003cp\u003eAll components are stored at -20°C, and the validity period is one year.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-node=\"619a7d46d61a5\" class=\"fl-row fl-row-fixed-width fl-row-bg-none fl-node-619a7d46d61a5\"\u003e\n\u003cdiv class=\"fl-row-content-wrap\"\u003e\n\u003cdiv class=\"fl-row-content fl-row-fixed-width fl-node-content\"\u003e\n\u003cdiv data-node=\"619a7d46d61e4\" class=\"fl-col-group fl-node-619a7d46d61e4\"\u003e\n\u003cdiv data-node=\"619a7d46d6224\" class=\"fl-col fl-node-619a7d46d6224\"\u003e\n\u003cdiv class=\"fl-col-content fl-node-content\"\u003e\n\u003cdiv data-animation-delay=\"0.0\" data-node=\"619a7d46d6263\" class=\"fl-module fl-module-heading fl-node-619a7d46d6263 pro_tit\"\u003e\n\u003cdiv class=\"fl-module-content fl-node-content\"\u003e\n\u003ch3 class=\"fl-heading\"\u003e\u003cspan class=\"fl-heading-text\"\u003eProduct Introduction\u003c\/span\u003e\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-node=\"619a7d46d62a8\" class=\"fl-col-group fl-node-619a7d46d62a8\"\u003e\n\u003cdiv data-node=\"619a7d46d62f3\" class=\"fl-col fl-node-619a7d46d62f3\"\u003e\n\u003cdiv class=\"fl-col-content fl-node-content\"\u003e\n\u003cdiv data-animation-delay=\"0.0\" data-node=\"619a7d46d6347\" class=\"fl-module fl-module-rich-text fl-node-619a7d46d6347\"\u003e\n\u003cdiv class=\"fl-module-content fl-node-content\"\u003e\n\u003cdiv class=\"fl-rich-text\"\u003e\n\u003cp\u003eMultiplex PCR is a PCR technology that allows multiple gene loci to be amplified simultaneously in a single PCR reaction. It has been widely used in scientific research and disease diagnosis and other fields, and is especially suitable for multi-site detection of small samples. The kit is based on the special hot-start polymerase Multiplex DNA Polymerase for multiplex PCR, providing the highest amplification specificity and sensitivity. Equipped with the 2 × Multiplex Buffer optimized for multiplex PCR, the Multiplex PCR Kit can complete most multiplex PCR detections under default conditions, and has a wide range of adaptability, which minimizes the steps of reaction system optimization.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-node=\"619a7d46d638e\" class=\"fl-row fl-row-fixed-width fl-row-bg-none fl-node-619a7d46d638e\"\u003e\n\u003cdiv class=\"fl-row-content-wrap\"\u003e\n\u003cdiv class=\"fl-row-content fl-row-fixed-width fl-node-content\"\u003e\n\u003cdiv data-node=\"619a7d46d63c6\" class=\"fl-col-group fl-node-619a7d46d63c6\"\u003e\n\u003cdiv data-node=\"619a7d46d6409\" class=\"fl-col fl-node-619a7d46d6409\"\u003e\n\u003cdiv class=\"fl-col-content fl-node-content\"\u003e\n\u003cdiv data-animation-delay=\"0.0\" data-node=\"619a7d46d6447\" class=\"fl-module fl-module-heading fl-node-619a7d46d6447 pro_tit\"\u003e\n\u003cdiv class=\"fl-module-content fl-node-content\"\u003e\n\u003ch3 class=\"fl-heading\"\u003e\u003cspan class=\"fl-heading-text\"\u003eProduct Advantages\u003c\/span\u003e\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-node=\"619a7d46d6485\" class=\"fl-col-group fl-node-619a7d46d6485\"\u003e\n\u003cdiv data-node=\"619a7d46d64c2\" class=\"fl-col fl-node-619a7d46d64c2\"\u003e\n\u003cdiv class=\"fl-col-content fl-node-content\"\u003e\n\u003cdiv data-animation-delay=\"0.0\" data-node=\"619a7d46d64ff\" class=\"fl-module fl-module-rich-text fl-node-619a7d46d64ff\"\u003e\n\u003cdiv class=\"fl-module-content fl-node-content\"\u003e\n\u003cdiv class=\"fl-rich-text\"\u003e\n\u003cp\u003eMultiplicity: the detection multiplicity can reach 19 or even higher;\u003cbr\u003eUniformity: Amplification is stable and uniform, with very low target preference;\u003cbr\u003eAdaptability: It is widely compatible with amplicons of various GC content and length, and GC Enhancer is included with the enzyme;\u003cbr\u003eSpecificity: The amplification buffer is highly optimized to minimize non-specific amplification;\u003cbr\u003eSensitivity: Using genomic DNA as a template, the amplification sensitivity can reach 1 ng.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Gene Bio Systems","offers":[{"title":"50 Rxn","offer_id":40259114795108,"sku":"PM101-01","price":227.35,"currency_code":"CAD","in_stock":true},{"title":"200 Rxn","offer_id":40259114827876,"sku":"PM101-02","price":760.78,"currency_code":"CAD","in_stock":true},{"title":"1000 Rxn","offer_id":40259114860644,"sku":"PM101-03","price":3250.14,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_b70cf6bc-1f4a-4eeb-9f42-1e7369cb18ba.jpg?v=1706882190"},{"product_id":"transstart-r-fastpfu-fly-dna-polymerase","title":"TransStart® FastPfu Fly DNA Polymerase","description":"\u003cdiv class=\"acontentc\"\u003e\n\u003cp\u003e\u003cem\u003eTransStart\u003c\/em\u003e®\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eFastPfu\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eFly DNA Polymerase is a hot start, high-fidelity and high processivity DNA Polymerase used for fast PCR. Compared with\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eTransStart\u003c\/em\u003e®\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eFastPfu\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eDNA Polymerase,\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eTransStart\u003c\/em\u003e®\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eFastPfu\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eFly DNA Polymerase has higher extension rate (6kb\/min vs 4kb\/min), featuring higher amplification efficiency, higher yield, higher fidelity and higher specificity.2×\u003cem\u003eTransStart\u003c\/em\u003e® FastPfu Fly Reaction Mix already contains dNTPs. When DNA is amplified, just add template, primer, water and\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eTransStart\u003c\/em\u003e®\u003cem\u003e\u003cspan\u003e \u003c\/span\u003eFastPfu\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003eFly DNA Polymerase. The Reaction can be carried out with the concentration of Reaction Mix being 1×. PCR products are blunt end and can be cloned directly into\u003cspan\u003e \u003c\/span\u003e\u003cem\u003epEASY\u003c\/em\u003e®-Blunt series of vectors.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e• Offers 108-fold fidelity as compared to\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eEasyTaq\u003c\/em\u003e® DNA Polymerase.\u003c\/p\u003e\n\u003cp\u003e• PCR products can be directly cloned into\u003cspan\u003e \u003c\/span\u003e\u003cem\u003epEASY\u003c\/em\u003e®-Blunt vectors. \u003c\/p\u003e\n\u003cp\u003e• Amplification of genomic DNA fragment up to 15 kb. \u003c\/p\u003e\n\u003cp\u003e• Amplification of plasmid DNA fragment up to 20 kb.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e• Hot start, high specificity.\u003c\/p\u003e\n\u003cp\u003e• High amplification efficiency.\u003c\/p\u003e\n\u003cp\u003e• Fast and high fidelity.\u003c\/p\u003e\n\u003cp\u003e• High sensitivity.\u003c\/p\u003e\n\u003cp\u003e• High production.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003cbr\u003eat -20 ℃ for two years\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eShipping\u003c\/strong\u003e\u003cbr\u003eDry ice (-70 ℃)\u003cbr\u003e\u003c\/p\u003e\n\u003cdiv class=\"clear\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"acontentc\"\u003e\n\u003cdiv class=\"clear\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cspan class=\"author\"\u003e\u003cstrong\u003eProduct Contents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"acontentc\"\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ctable border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr class=\"firstRow\"\u003e\n\u003ctd\u003eComponent\u003c\/td\u003e\n\u003ctd\u003eAP231-21-V3\u003c\/td\u003e\n\u003ctd\u003eAP231-22-V3\u003c\/td\u003e\n\u003ctd\u003eAP231-23-V3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eTransStart\u003c\/em\u003e\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eFastPfu\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eFly DNA Polymerase\u003c\/td\u003e\n\u003ctd\u003e250 U×1\u003c\/td\u003e\n\u003ctd\u003e500 U×1\u003c\/td\u003e\n\u003ctd\u003e500 U×6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2×\u003cem\u003eTransStart\u003c\/em\u003e\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eFastPfu Fly Reaction\u003c\/td\u003e\n\u003ctd\u003e1 ml×3\u003c\/td\u003e\n\u003ctd\u003e1 ml ×6\u003c\/td\u003e\n\u003ctd\u003e1 ml ×36\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e50 mM MgSO\u003csub\u003e4\u003c\/sub\u003e\n\u003c\/td\u003e\n\u003ctd\u003e200 μl×1\u003c\/td\u003e\n\u003ctd\u003e400 μl ×1\u003c\/td\u003e\n\u003ctd\u003e1 ml ×1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePCR Stimulant\u003c\/td\u003e\n\u003ctd\u003e200 μl×1\u003c\/td\u003e\n\u003ctd\u003e400 μl×1\u003c\/td\u003e\n\u003ctd\u003e1 ml ×1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e6×DNA Loading Buffer\u003c\/td\u003e\n\u003ctd\u003e500 μl×1\u003c\/td\u003e\n\u003ctd\u003e1 ml ×1\u003c\/td\u003e\n\u003ctd\u003e1 ml ×2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cdiv class=\"clear\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/AP231_2023-03-05-GBS.pdf?v=1700584260\"\u003e\u003cimg height=\"43\" width=\"153\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\"\u003e\u003c\/a\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cspan class=\"author\"\u003e\u003cstrong\u003eCitations\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"acontentc\"\u003e\n\u003cp\u003e\u003cspan\u003eZhu X J , Sun S , Xie B , \u003cem\u003eet al\u003c\/em\u003e. Guanine-rich sequences inhibit proofreading DNA polymerases[J]. Scientific Reports, 2016, 6:28769.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eYe F , Lan X E , Zhu W B , \u003cem\u003eet al\u003c\/em\u003e. Mitochondrial genomes of praying mantises (Dictyoptera, Mantodea): rearrangement, duplication, and reassignment of tRNA genes[J]. Scientific Reports, 2016, 6:25634.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eHu L , Li H , Qin R ,\u003cem\u003e et al\u003c\/em\u003e. Plant phosphomannose isomerase as a selectable marker for rice transformation[J]. Scientific Reports, 2016, 6(1):25921.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eChao H , Zhou N Y . GenR, an IclR-Type Regulator, Activates and Represses the Transcription of gen Genes Involved in 3-Hydroxybenzoate and Gentisate Catabolism in Corynebacterium glutamicum[J]. Journal of Bacteriology, 2013, 195(7):1598-1609.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eLiu X, Pu J, Zeng S, \u003cem\u003eet al\u003c\/em\u003e. Hyriopsis cumingii Hic52-A novel nacreous layer matrix protein with a collagen-like structure[J].Int J Biol Macromol. 2017 Sep;102:667-673.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Gene Bio Systems","offers":[{"title":"250 Units |","offer_id":41524913438820,"sku":"AP231-01","price":342.32,"currency_code":"CAD","in_stock":true},{"title":"500 Units   |","offer_id":41524913471588,"sku":"AP231-02","price":615.69,"currency_code":"CAD","in_stock":true},{"title":"6x500 Units","offer_id":41524913504356,"sku":"AP231-03","price":3142.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/TransStartFastPFUFlybox.png?v=1676739537"},{"product_id":"2-rapid-taq-master-mix-p222","title":"2 × Rapid Taq Master Mix P222","description":"\u003cdiv data-node=\"619a7abe51ed8\" class=\"fl-row fl-row-fixed-width fl-row-bg-none fl-node-619a7abe51ed8\"\u003e\n\u003cdiv class=\"fl-row-content-wrap\"\u003e\n\u003cdiv class=\"fl-row-content fl-row-fixed-width fl-node-content\"\u003e\n\u003cdiv data-node=\"619a7abe51f18\" class=\"fl-col-group fl-node-619a7abe51f18\"\u003e\n\u003cdiv data-node=\"619a7abe51f58\" class=\"fl-col fl-node-619a7abe51f58\"\u003e\n\u003cdiv class=\"fl-col-content fl-node-content\"\u003e\n\u003cdiv data-animation-delay=\"0.0\" data-node=\"619a7abe51f99\" class=\"fl-module fl-module-heading fl-node-619a7abe51f99 pro_tit\"\u003e\n\u003cdiv class=\"fl-module-content fl-node-content\"\u003e\n\u003ch3 class=\"fl-heading\"\u003e\u003cspan class=\"fl-heading-text\"\u003eProduct Introduction\u003c\/span\u003e\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-animation-delay=\"0.0\" data-node=\"63b52907405c7\" class=\"fl-module fl-module-rich-text fl-node-63b52907405c7\"\u003e\n\u003cdiv class=\"fl-module-content fl-node-content\"\u003e\n\u003cdiv class=\"fl-rich-text\"\u003e\n\u003cp\u003e2 × Rapid Taq Master Mix contains Taq DNA Polymerase, elongation promoting factor, dNTP and an optimized buffer system. The amplification speed of this product can reach 15 sec\/kb, which is suitable for rapid PCR. The maximum amplification speed within 1 kb can reach 1 sec\/kb, greatly saving reaction time. The pre-prepared 2 × Master Mix only needs to add primers and templates to perform amplification, which reduces pipetting operations and improves detection throughput and results reproducibility. The kit has excellent amplification performance and high storage stability. It is suitable for PCR amplification within 5 kb using genome as template and PCR amplification within 10 kb using plasmid and λDNA as template. The protective agent added to the system allows 2 × Master Mix to maintain stable activity after repeated freezing and thawing. The kit provides a version containing electrophoresis buffer and green loading dye, which can be directly electrophoresed after the reaction that is convenient to use. The PCR product has an adenine at the 3' end, which can be directly cloned into T vectors and is suitable for ClonExpress and TOPO cloning kits (GeneBio #C112\/C113\/C115\/C601).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-node=\"619a7abe52098\" class=\"fl-row fl-row-fixed-width fl-row-bg-none fl-node-619a7abe52098\"\u003e\n\u003cdiv class=\"fl-row-content-wrap\"\u003e\n\u003cdiv class=\"fl-row-content fl-row-fixed-width fl-node-content\"\u003e\n\u003cdiv data-node=\"619a7abe520d8\" class=\"fl-col-group fl-node-619a7abe520d8\"\u003e\n\u003cdiv data-node=\"619a7abe52118\" class=\"fl-col fl-node-619a7abe52118\"\u003e\n\u003cdiv class=\"fl-col-content fl-node-content\"\u003e\n\u003cdiv data-animation-delay=\"0.0\" data-node=\"619a7abe52161\" class=\"fl-module fl-module-heading fl-node-619a7abe52161 pro_tit\"\u003e\n\u003cdiv class=\"fl-module-content fl-node-content\"\u003e\n\u003ch3 class=\"fl-heading\"\u003e\u003cspan class=\"fl-heading-text\"\u003eProduct Advantages\u003c\/span\u003e\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-node=\"619a7abe521a1\" class=\"fl-col-group fl-node-619a7abe521a1\"\u003e\n\u003cdiv data-node=\"619a7abe521e1\" class=\"fl-col fl-node-619a7abe521e1\"\u003e\n\u003cdiv class=\"fl-col-content fl-node-content\"\u003e\n\u003cdiv data-animation-delay=\"0.0\" data-node=\"619a7abe52222\" class=\"fl-module fl-module-rich-text fl-node-619a7abe52222\"\u003e\n\u003cdiv class=\"fl-module-content fl-node-content\"\u003e\n\u003cdiv class=\"fl-rich-text\"\u003e\n\u003cp\u003eFast amplification speed: 15 sec\/kb, the limit amplification speed can reach 1 sec\/kb within 1 kb;\u003cbr\u003eConvenient operation: amplification can be carried out by adding primers and templates, no operation on ice is required, and the product containing dye can be directly subjected to electrophoresis;\u003cbr\u003eGood stability: repeated freezing and thawing for 50 times, no significant decrease in activity\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-node=\"628201085d9ce\" class=\"fl-row fl-row-fixed-width fl-row-bg-none fl-node-628201085d9ce\"\u003e\n\u003cdiv class=\"fl-row-content-wrap\"\u003e\n\u003cdiv class=\"fl-row-content fl-row-fixed-width fl-node-content\"\u003e\n\u003cdiv data-node=\"628201085e145\" class=\"fl-col-group fl-node-628201085e145\"\u003e\n\u003cdiv data-node=\"628201085e18e\" class=\"fl-col fl-node-628201085e18e\"\u003e\n\u003cdiv class=\"fl-col-content fl-node-content\"\u003e\n\u003cdiv data-animation-delay=\"0.0\" data-node=\"628201085e1d0\" class=\"fl-module fl-module-heading fl-node-628201085e1d0 pro_tit\"\u003e\n\u003cdiv class=\"fl-module-content fl-node-content\"\u003e\n\u003ch3 class=\"fl-heading\"\u003e\u003cspan class=\"fl-heading-text\"\u003eComponents\u003c\/span\u003e\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-node=\"628201085e211\" class=\"fl-col-group fl-node-628201085e211\"\u003e\n\u003cdiv data-node=\"628201085e255\" class=\"fl-col fl-node-628201085e255\"\u003e\n\u003cdiv class=\"fl-col-content fl-node-content\"\u003e\n\u003cdiv data-animation-delay=\"0.0\" data-node=\"628201085e29d\" class=\"fl-module fl-module-rich-text fl-node-628201085e29d\"\u003e\n\u003cdiv class=\"fl-module-content fl-node-content\"\u003e\n\u003cdiv class=\"fl-rich-text\"\u003e\n\u003cp\u003e\u003cimg width=\"1000\" src=\"https:\/\/www.vazymebiotech.com\/uploads\/P222-Components.png\" alt=\"P222 Components\" class=\"aligncenter size-full wp-image-4008\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-node=\"619a7abe51d28\" class=\"fl-row fl-row-fixed-width fl-row-bg-none fl-node-619a7abe51d28\"\u003e\n\u003cdiv class=\"fl-row-content-wrap\"\u003e\n\u003cdiv class=\"fl-row-content fl-row-fixed-width fl-node-content\"\u003e\n\u003cdiv data-node=\"619a7abe51d67\" class=\"fl-col-group fl-node-619a7abe51d67\"\u003e\n\u003cdiv data-node=\"619a7abe51dae\" class=\"fl-col fl-node-619a7abe51dae\"\u003e\n\u003cdiv class=\"fl-col-content fl-node-content\"\u003e\n\u003cdiv data-animation-delay=\"0.0\" data-node=\"619a7abe51deb\" class=\"fl-module fl-module-heading fl-node-619a7abe51deb pro_tit\"\u003e\n\u003cdiv class=\"fl-module-content fl-node-content\"\u003e\n\u003ch3 class=\"fl-heading\"\u003e\u003cspan class=\"fl-heading-text\"\u003eStorage\u003c\/span\u003e\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-node=\"619a7abe51e29\" class=\"fl-col-group fl-node-619a7abe51e29\"\u003e\n\u003cdiv data-node=\"619a7abe51e66\" class=\"fl-col fl-node-619a7abe51e66\"\u003e\n\u003cdiv class=\"fl-col-content fl-node-content\"\u003e\n\u003cdiv data-animation-delay=\"0.0\" data-node=\"619a7abe51e96\" class=\"fl-module fl-module-rich-text fl-node-619a7abe51e96\"\u003e\n\u003cdiv class=\"fl-module-content fl-node-content\"\u003e\n\u003cdiv class=\"fl-rich-text\"\u003e\n\u003cp\u003eStore at -20℃  for up to 18 months; can be stored at 4℃ for 4 months after thawing\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-node=\"62981860ae81a\" class=\"fl-row fl-row-fixed-width fl-row-bg-none fl-node-62981860ae81a\"\u003e\n\u003cdiv class=\"fl-row-content-wrap\"\u003e\n\u003cdiv class=\"fl-row-content fl-row-fixed-width fl-node-content\"\u003e\n\u003cdiv data-node=\"62981860aeee4\" class=\"fl-col-group fl-node-62981860aeee4\"\u003e\n\u003cdiv data-node=\"62981860aef2a\" class=\"fl-col fl-node-62981860aef2a\"\u003e\n\u003cdiv class=\"fl-col-content fl-node-content\"\u003e\n\u003cdiv data-animation-delay=\"0.0\" data-node=\"62981860aef6a\" class=\"fl-module fl-module-heading fl-node-62981860aef6a pro_tit\"\u003e\n\u003cdiv class=\"fl-module-content fl-node-content\"\u003e\n\u003ch3 class=\"fl-heading\"\u003e\u003cspan class=\"fl-heading-text\"\u003eCitation\u003c\/span\u003e\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-node=\"62981860aefac\" class=\"fl-col-group fl-node-62981860aefac\"\u003e\n\u003cdiv data-node=\"62981860aefed\" class=\"fl-col fl-node-62981860aefed\"\u003e\n\u003cdiv class=\"fl-col-content fl-node-content\"\u003e\n\u003cdiv data-animation-delay=\"0.0\" data-node=\"62981860af02d\" class=\"fl-module fl-module-rich-text fl-node-62981860af02d\"\u003e\n\u003cdiv class=\"fl-module-content fl-node-content\"\u003e\n\u003cdiv class=\"fl-rich-text\"\u003e\n\u003cp\u003eHuang, Jianfa, et al. \"Migration of pre-induced human peripheral blood mononuclear cells from the transplanted to contralateral eye in mice.\" Stem cell research \u0026amp; therapy 12.1 (2021): 1-13.\u003c\/p\u003e\n\u003cp\u003eWang, Tianren, et al. \"MFN2 Deficiency Impairs Mitochondrial Functions and PPAR Pathway During Spermatogenesis and Meiosis in Mice.\" Frontiers in cell and developmental biology 10 (2022).\u003c\/p\u003e\n\u003cp\u003eCao, Qiqi, et al. \"The Recurrent Mutation in PATL2 Inhibits Its Degradation Thus Causing Female Infertility Characterized by Oocyte Maturation Defect Through Regulation of the Mos-MAPK Pathway.\" Frontiers in cell and developmental biology (2021): 116.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-animation-delay=\"0.0\" data-node=\"6368cf814c4df\" class=\"fl-module fl-module-heading fl-node-6368cf814c4df pro_tit\"\u003e\n\u003cdiv class=\"fl-module-content fl-node-content\"\u003e\n\u003ch3 class=\"fl-heading\"\u003e\u003cspan class=\"fl-heading-text\"\u003eFAQ\u003c\/span\u003e\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-animation-delay=\"0.0\" data-node=\"6368cf83378ce\" class=\"fl-module fl-module-rich-text fl-node-6368cf83378ce\"\u003e\n\u003cdiv class=\"fl-module-content fl-node-content\"\u003e\n\u003cdiv class=\"fl-rich-text\"\u003e\n\u003cp\u003e\u003cstrong\u003eQ1: The amplification efficiency is low and there are no amplified bands in the test group.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eA1: (1) Primers\u003c\/p\u003e\n\u003cp\u003eCheck whether the synthetic primers have degraded due to improper storage; review primer design and use BLAST to examine primer specificity or redesign primers.\u003c\/p\u003e\n\u003cp\u003e(2) Template\u003c\/p\u003e\n\u003cp\u003eLong-term storage or repeated freeze-thaw cycles will lead to DNA breakage, nicking, or degradation, so the template should be the freshly prepared double-stranded DNA. An excessively high GC content in the template will make it difficult to separate DNA double strands; in this case, add PCR Enhancer (Cat. No. P021) to effectively lower the melting temperature. Crude sample templates may contain inhibitors, so it is recommended to lower the template concentration (use after dilution; if a plant leaf is used, make sure the plant is not rich in polysaccharides or polyphenols, take a fresh, young leaf, and trim it to the size of the end of a yellow pipette tip). If cDNA is used, check the purity and integrity of the RNA used for reverse transcription.\u003c\/p\u003e\n\u003cp\u003e(3) Enzyme\u003c\/p\u003e\n\u003cp\u003eThe enzyme used in the reaction has been inactivated. Repeat the experiment with a fresh enzyme or an enzyme from another source.\u003c\/p\u003e\n\u003cp\u003e(4) Amplification system\u003c\/p\u003e\n\u003cp\u003eThe reaction system has been prepared incorrectly. Repeat the experiment with the correct system.\u003c\/p\u003e\n\u003cp\u003e(5) Reaction program\u003c\/p\u003e\n\u003cp\u003eCheck whether the denaturation temperature is correct and whether the temperature displayed on the PCR system is the same as the actual temperature. If the temperature is too high, the enzyme will be rapidly inactivated in the first few cycles. If the temperature is too low, the template will not be completely denatured. If yeast is used as the template, the pre-denaturation time may be extended to 10 minutes. If the annealing temperature is inappropriate, determine the appropriate annealing temperature by testing a gradient of annealing temperatures. If the target fragment is long, try the touchdown PCR program. Check whether the extension time is sufficient.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eQ2: The amplified bands are faint.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eA2: (1) Primers\u003c\/p\u003e\n\u003cp\u003eCheck whether the synthetic primers have degraded.\u003c\/p\u003e\n\u003cp\u003e(2) Template\u003c\/p\u003e\n\u003cp\u003eCheck the quality of the template. Long-term storage or repeated freeze-thaw cycles will lead to DNA breakage, nicking, or degradation, so the freshly prepared double-stranded DNA should be used as the template. If the template has been used up, use serial dilutions of the initial amplification product as the templates for subsequent amplification.\u003c\/p\u003e\n\u003cp\u003e(3) Reaction program\u003c\/p\u003e\n\u003cp\u003eTry the touchdown PCR program; increase the extension time and the number of cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eQ3: Amplification specificity is poor, or there are non-specific products.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eA3: (1) Primers\u003c\/p\u003e\n\u003cp\u003ePrimer design is not optimal. If primers have bound to non-specific sites in the target sequence or to each other to form dimers, optimize by reducing the primer concentration or redesigning primers if necessary.\u003c\/p\u003e\n\u003cp\u003e(2) Template\u003c\/p\u003e\n\u003cp\u003eIf the template is impure or contaminated, prepare a new template.\u003c\/p\u003e\n\u003cp\u003eIf the template has degraded, or too much template has been used, examine the integrity and concentration of the template by electrophoresis, and purify the template again if necessary. Consult the Instructions for Use for the template input amount.\u003c\/p\u003e\n\u003cp\u003e(3) Reaction program\u003c\/p\u003e\n\u003cp\u003eThe reaction program is not optimal. If there are non-specific miscellaneous bands smaller than the target band, increase the annealing temperature and reduce the number of cycles. If there are non-specific miscellaneous bands larger than the target band, reduce the extension time and the number of cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eQ4: The bands are diffuse or smeared when amplification products are run on a gel.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eA4: (1) Gel\u003c\/p\u003e\n\u003cp\u003eMelt the gel completely during preparation.\u003c\/p\u003e\n\u003cp\u003e(2) Primers\u003c\/p\u003e\n\u003cp\u003eCheck whether the primers have degraded.\u003c\/p\u003e\n\u003cp\u003e(3) Template\u003c\/p\u003e\n\u003cp\u003eThe template is degraded or excessive. Examine the integrity and concentration of the template by electrophoresis, and prepare a new template if necessary. Consult the Instructions for Use for the template input amount. If the target band is long and the cDNA is used as the template, check the purity and integrity of the RNA used for reverse transcription, and perform reverse transcription again without random primers.\u003c\/p\u003e\n\u003cp\u003e(4) Reaction program\u003c\/p\u003e\n\u003cp\u003eThe reaction program is not optimal. If the annealing temperature is inappropriate, determine the appropriate annealing temperature by testing a gradient of annealing temperatures. Try the touchdown PCR program.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eQ5: There are amplification products in the blank control (NTC) group.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eA5: (1) Problematic primer design\u003c\/p\u003e\n\u003cp\u003eThe amplified sequence is homologous to the non-target sequence. Thus, non-target sequences are also amplified in PCR.\u003c\/p\u003e\n\u003cp\u003e(2) If the band corresponding to the amplification product has the same size as the target bands, this indicates contamination.\u003c\/p\u003e\n\u003cp\u003eRepeat the experiment with a fresh Mix, water, or primers. Prepare the reaction system on an ultra-clean workbench to minimize aerosol contamination.\u003c\/p\u003e\n\u003cp\u003e(3) To avoid cross-contamination of the target sequence by the genome or large fragments, perform the steps with care to prevent the target sequence from being pipetted into the loading pipette or spilling from centrifuge tubes.\u003c\/p\u003e\n\u003cp\u003e(4) To avoid contamination of the target gene by small airborne nucleic acid fragments, use the nested PCR method to reduce or eliminate contamination.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eQ6: Do dyed PCR products affect restriction digestion?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eA6: Tests show that dyed PCR products do not affect restriction digestion.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/P222_2x_Rapid_Taq_Master_Mix-GBS_Manual_-_Nov_2024.pdf?v=1732115653\" target=\"_blank\" title=\"P222 Manual - GBS Nov 2024\" rel=\"noopener\"\u003e\u003cimg height=\"42\" width=\"149\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" data-mce-selected=\"1\"\u003e\u003c\/a\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/P222_2_u_Rapid_Taq_Master_Mix-GBS.pdf?v=1700835217\"\u003e\u003c\/a\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Gene Bio Systems","offers":[{"title":"5mL","offer_id":41735546896484,"sku":"P222-01","price":187.49,"currency_code":"CAD","in_stock":true},{"title":"15mL","offer_id":41735546929252,"sku":"P222-02","price":518.92,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_863feffb-2c32-4a58-89ba-4a225ea3b0b1.jpg?v=1706882074"},{"product_id":"hyperactive-pg-mnase-cut-run-assay-kit-for-pcr-qpcr","title":"Hyperactive pG-MNase CUT\u0026RUN Assay Kit for PCR\/qPCR","description":"\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e Hyperactive pG-MNase CUT\u0026amp;RUN Assay Kit for PCR\/qPCR is developed specially for studying protein-DNA interactions. Cleavage Under Targets and Release Using Nuclease (CUT\u0026amp;RUN) is a new method for studying protein-DNA interactions. By fusing Protein G with transposase, under the guidance of the antibody, it allows accurate targeting of the target protein and cutting of the DNA sequence near the target site. With an optimized reaction system and workflow, this kit offers multiple advantages over conventional ChIP-qPCR, including high success rate, excellent antibody compatibility, short turnaround time, and ease of use, making It is especially suitable for such fields as early embryonic development, stem cells, cancer, and epigenetics. All reagents in this kit have undergone strict quality control and function testing to ensure the optimal stability and repeatability of experimental results.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eApplications\u003cbr\u003e06-1\/About qPCR Reagents\u003cbr\u003eThis product is intended for studying protein-DNA interactions in mammalian cells with\u003cbr\u003e5,000 - 500,000 input cells. Yeast, plant tissues and cells should be treated before proceeding\u003cbr\u003ewith the assay using this kit.\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"24 rxn","offer_id":41740635570276,"sku":"HD101-01","price":1003.87,"currency_code":"CAD","in_stock":true},{"title":"48 rxn","offer_id":41740635603044,"sku":"HD101-02","price":1743.57,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/hd101image.png?v=1683497614"},{"product_id":"2-phanta-flash-master-mix-dye-plus-p520","title":"2 × PaCeR (Phanta) Flash Master Mix (Dye Plus)","description":"\u003cp\u003e2 × Phanta Flash Master Mix (Dye Plus) is a new generation superior enzyme based on Phanta Flash Super-Fidelity DNA Polymerase. Through directed optimization of Phanta DNA Polymerase,Phanta Flash Super-Fidelity DNA Polymerase has the characteristics of rapid amplification (4 - 5 sec\/kb) while maintaining high fidelity and yield. Matched with optimized buffer system, this kit can achieve high amplification specificity. And it has excellent compatibility with crude samples, templates with uracil and GC-rich system (primer\/template). This kit contains two types of monoclonal antibodies that inhibit the 5'→3' polymerase activity and 3'→5' exonuclease activity at room temperature, enabling it to perform hot start PCR with great specificity. It contains all required reaction components (Phanta Flash Super-Fidelity DNA Polymerase, dNTP and optimized buffer), except primers and templates, thereby simplifying the operation process and improving the detection throughput and repeatability. This kit contains loading buffer, which can be directly spotted for electrophoresis after the reaction. Amplification will generate blunt-ended products, which are compatible with ClonExpress kits (#C112\/C113\/C115) and TOPO cloning kit.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eHigh fidelity: ~106X higher than Taq DNA Polymerase\u003c\/li\u003e\n\u003cli\u003eFlash amplification: \u003cspan data-olk-copy-source=\"MessageBody\"\u003eThe fastest amplification speed it supports is as fast as 5 sec\/kb\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003eRobust performance: Superior specificity, sensitivity, and yields\u003c\/li\u003e\n\u003cli\u003eBroad compatibility: Suitable for complex samples \u0026amp; GC-rich systems\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/P510_fast.png?v=1719510764\" alt=\"\" width=\"805\" height=\"969\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/PaCeR_Phanta_Flash_MM-P520_Manual-V23.1-GBS.pdf?v=1702410817\"\u003e\u003cspan\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"185\" height=\"52\"\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"1mL","offer_id":41800624570468,"sku":"P520-01","price":160.68,"currency_code":"CAD","in_stock":true},{"title":"5mL","offer_id":41800624603236,"sku":"P520-02","price":650.01,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/Screenshot_98.png?v=1685386583"},{"product_id":"perfectstart-r-taq-dna-polymerase","title":"PerfectStart® Taq DNA Polymerase","description":"\u003cdiv class=\"acontentc\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cem data-mce-fragment=\"1\"\u003ePerfectStart\u003c\/em\u003e\u003csup data-mce-fragment=\"1\"\u003e®\u003c\/sup\u003e Taq DNA Polymerase is a hot start Taq DNA polymerase containing Taq DNA polymerase and three kinds of monoclonal antibodies, effectively blocking DNA polymerase activity and preventing non-specific amplification at low temperature. As the denaturation step progresses, the antibodies are inactivated and Taq DNA Polymerase is activated, which can effectively enhance the amplification efficiency and improve the amplification sensitivity and specificity. The amplified product has an \"A\" base at the 3' end and can be directly cloned into the \u003cem data-mce-fragment=\"1\"\u003epEASY\u003c\/em\u003e\u003csup data-mce-fragment=\"1\"\u003e®\u003c\/sup\u003e-T vectors.\u003cbr data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e• Compared with \u003cem data-mce-fragment=\"1\"\u003eTransStart\u003c\/em\u003e\u003csup data-mce-fragment=\"1\"\u003e®\u003c\/sup\u003e Taq DNA Polymerase and \u003cem data-mce-fragment=\"1\"\u003eTransStart\u003c\/em\u003e\u003csup data-mce-fragment=\"1\"\u003e®\u003c\/sup\u003e Top Taq DNA Polymerase, \u003cem data-mce-fragment=\"1\"\u003ePerfectStart\u003c\/em\u003e\u003csup data-mce-fragment=\"1\"\u003e®\u003c\/sup\u003e Taq DNA Polymerase has higher amplification efficiency, specificity and sensitivity.\u003cbr data-mce-fragment=\"1\"\u003e• Prepared at room temperature, reduced nonspecific and primer dimer formation.\u003cbr data-mce-fragment=\"1\"\u003e• Different from chemical modification, long denaturing step is not needed.\u003cbr data-mce-fragment=\"1\"\u003e• The reaction solution is specifically designed for molecular diagnosis, and the amplification length is no more than 1 kb.\u003cbr data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e• Hot start and high specificity.\u003cbr data-mce-fragment=\"1\"\u003e• High sensitivity.\u003cbr data-mce-fragment=\"1\"\u003e• High amplification efficiency.\u003cbr data-mce-fragment=\"1\"\u003e• Low E. coli genomic DNA residue.\u003cbr data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eApplications\u003c\/strong\u003e\u003cbr data-mce-fragment=\"1\"\u003e• Low copy number templates.\u003cbr data-mce-fragment=\"1\"\u003e• Complex templates.\u003cbr data-mce-fragment=\"1\"\u003e• GC\/AT-rich templates.\u003cbr data-mce-fragment=\"1\"\u003e• Real-time Quantitative PCR (qPCR).\u003cbr data-mce-fragment=\"1\"\u003e• Multiplex PCR.\u003cbr data-mce-fragment=\"1\"\u003e• Single nucleotide polymorphisms ( SNPs)\u003cbr data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003cbr data-mce-fragment=\"1\"\u003eat -20 ℃ for two years\u003cbr data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eShipping\u003c\/strong\u003e\u003cbr data-mce-fragment=\"1\"\u003eDry ice (-70 ℃)\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003cdiv class=\"clear\" data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"acontentc\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"clear\" data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cspan class=\"author\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProduct Contents\u003c\/strong\u003e\u003c\/span\u003e\n\u003cdiv class=\"acontentc\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr class=\"firstRow\" data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eComponent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eAP401-01\/11\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eAP401-02\/12\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eAP401-03\/13\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cem data-mce-fragment=\"1\"\u003ePerfectStart\u003c\/em\u003e® Taq DNA Polymerase\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e250 U×1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e500 U×1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e500 U×6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e10×\u003cem data-mce-fragment=\"1\"\u003ePerfectStart\u003c\/em\u003e®Taq Buffer\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1.2 ml×1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1.2 ml×2\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1.2 ml×12\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5 mM dNTPs\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e-\/800 μl×1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e-\/800 μl×2\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e-\/1.2 ml×8\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e6×DNA Loading Buffer\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e500 μl×1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1 ml×1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1 ml×2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e10×GC Enhancer\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e200 μl×1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e400 μl×1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1 ml×1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e","brand":"GeneBio Systems","offers":[{"title":"250Units","offer_id":41938245582948,"sku":"AP401-01","price":204.71,"currency_code":"CAD","in_stock":true},{"title":"500Units","offer_id":41938245615716,"sku":"AP401-02","price":379.09,"currency_code":"CAD","in_stock":true},{"title":"6×500Units","offer_id":41938245648484,"sku":"AP401-03","price":2084.32,"currency_code":"CAD","in_stock":true},{"title":"250Units (with dNTP)","offer_id":41938245681252,"sku":"AP401-04","price":220.63,"currency_code":"CAD","in_stock":true},{"title":"500Units (with dNTPs)","offer_id":41938245714020,"sku":"AP401-05","price":393.24,"currency_code":"CAD","in_stock":true},{"title":"6×500Units (with dNTPs)","offer_id":41938245746788,"sku":"AP401-06","price":2201.23,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_546bf71e-8677-4ae6-9984-6bf0bd65b961.jpg?v=1689277739"},{"product_id":"nuclease-free-water","title":"Nuclease-free Water","description":"\u003cp\u003e\u003cem\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003eNuclease-free water is RNase-free and DNase-free. it is ideal for the preparation of reagents and for use in enzymatic reactions, including PCR, ligation etc. \u003c\/p\u003e\n\u003cp\u003eThis water does not contain toxic agents, such as DEPC, which can inhibit enzymatic reactions\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eAvailable in 100 ml or 200 ml sizes. \u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Gene Bio Systems","offers":[{"title":"100 mL","offer_id":41947398307940,"sku":"WTRNF-01","price":83.46,"currency_code":"CAD","in_stock":true},{"title":"200 mL","offer_id":41947398340708,"sku":"WTRNF-032","price":152.41,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/nuclease_water-removebg-preview_b35afbec-780d-4991-8f31-98a77435b63f.png?v=1702051562"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/collections\/PCR_reagents_collection_image.png?v=1662144709","url":"https:\/\/www.genebiosystems.com\/en-jp\/collections\/pcr-reagents\/reverse-transcription.oembed?page=2","provider":"GeneBio ","version":"1.0","type":"link"}