{"product_id":"xrcc5-antibody-cat-csb-pa026233dsr2hu","title":"XRCC5 Antibody - Cat. #: CSB-PA026233DSR2HU","description":"\u003cp\u003e\u003cb\u003eSize \u003c\/b\u003e:50ul\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone Number\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAliases\u003c\/b\u003e:86 kDa subunit of Ku antigen antibody; ATP dependent DNA helicase 2 subunit 2 antibody; ATP dependent DNA helicase II 80 kDa subunit antibody; ATP dependent DNA helicase II 86 Kd subunit antibody; ATP dependent DNA helicase II antibody; ATP-dependent DNA helicase 2 subunit 2 antibody; ATP-dependent DNA helicase II 80 kDa subunit antibody; CTC box binding factor 85 kDa antibody; CTC box-binding factor 85 kDa subunit antibody; CTC85 antibody; CTCBF antibody; DNA repair protein XRCC5 antibody; KARP 1 antibody; KARP1 antibody; Ku 80 antibody; Ku autoantigen 80kDa antibody; Ku80 antibody; Ku86 antibody; Ku86 autoantigen related protein 1 antibody; KUB 2 antibody; KUB2 antibody; Lupus Ku autoantigen protein p86 antibody; NFIV antibody; Nuclear factor IV antibody; Thyroid lupus autoantigen antibody; Thyroid-lupus autoantigen antibody; TLAA antibody; X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining) antibody; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) antibody; X-ray repair cross-complementing protein 5 antibody; Xray repair complementing defective repair in Chinese hamster cells 5 antibody; XRCC 5 antibody; XRCC5 antibody; XRCC5_HUMAN antibody\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Type\u003c\/b\u003e:Polyclonal Antibody\u003c\/p\u003e\u003cp\u003e\u003cb\u003eImmunogen Species\u003c\/b\u003e:Homo sapiens (Human)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniProt ID\u003c\/b\u003e:P13010\u003c\/p\u003e\u003cp\u003e\u003cb\u003eImmunogen\u003c\/b\u003e:Recombinant Human X-ray repair cross-complementing protein 5 protein (543-732AA)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRaised in\u003c\/b\u003e:Rabbit\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecies Reactivity\u003c\/b\u003e:Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTested Applications\u003c\/b\u003e:ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e:Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3\\\\\\'-5\\\\\\' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5\/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5\/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5\/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5\/6 dimer probably also acts as a 5\\\\\\'-deoxyribose-5-phosphate lyase (5\\\\\\'-dRP lyase), by catalyzing the beta-elimination of the 5\\\\\\' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5\\\\\\'-dRP activity, and allows to \\\\\\'clean\\\\\\' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5\/6 dimer together with APEX1 acts as a negative regulator of transcription.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality\u003c\/b\u003e:Polyclonal\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e:IgG\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurification Method\u003c\/b\u003e:Antigen Affinity Purified\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugate\u003c\/b\u003e:Non-conjugated\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBuffer\u003c\/b\u003e:PBS with 0.02% sodium azide, 50% glycerol, pH7.3.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eForm\u003c\/b\u003e:Liquid\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStroage\u003c\/b\u003e:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Names\u003c\/b\u003e:XRCC5\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e:Epigenetics and Nuclear Signaling\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":40486065438820,"sku":"CSB-PA026233DSR2HU","price":287.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/no_image_default_image-jpeg_ac956df1-271d-41fa-9f8a-ba55a71c35ab.jpg?v=1660140230","url":"https:\/\/www.genebiosystems.com\/en-gb\/products\/xrcc5-antibody-cat-csb-pa026233dsr2hu","provider":"GeneBio ","version":"1.0","type":"link"}