{"product_id":"transdetect-r-annexin-v-pe-7-aad-cell-apoptosis-detection-kit","title":"TransDetect® Annexin V-PE\/7-AAD Cell Apoptosis Detection Kit","description":"\u003cspan class=\"author\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProduct Details\u003c\/strong\u003e\u003c\/span\u003e\n\u003cdiv class=\"acontentc\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eAnnexin V is one of the most sensitive indicators for early apoptosis detection. In normal cells, the phosphatidylserine (PS) is located on the inside of the phospholipid bilayer of the cell membrane. \u003cspan data-mce-fragment=\"1\"\u003eAt the early stages of apoptosis\u003c\/span\u003e, PS is translocated from the inner to the outer leaflet of the plasma membrane, thereby exposing PS to the external cellular environment. The Annexin V, a Ca\u003csup data-mce-fragment=\"1\"\u003e2\u003c\/sup\u003e\u003csup data-mce-fragment=\"1\"\u003e+\u003c\/sup\u003e dependent phospholipid-binding protein, can bind specifically to the exposed PS. Therefore, the nature of Annexin V binding to the PS exposed on the outside of cells can be used to detect the early apoptosis of the cells. Annexin V marked with R-Phycoerythrin (R-PE) is used as a probe to detect the occurrence of apoptosis using flow cytometry.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e7-AAD is a nucleic acid binding dye, which cannot pass through the normal cells with complete cell membrane and early apoptotic cells, but can stain the nucleus through the cell membrane of apoptotic cells in the middle and late period and necrotic cells, and can be excited by 488 nm with a maximum emission wavelength of 647 nm. By maching Annexin V with 7-AAD, cells in different stages of apoptosis can be distinguished. This kit is easy to operate, highly sensitive and specific, and is suitable for the detection of early apoptosis of the cells.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003cbr data-mce-fragment=\"1\"\u003eat 2-8℃ for one year.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eShipping\u003c\/strong\u003e\u003cbr data-mce-fragment=\"1\"\u003eIce bag (4℃).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cspan class=\"author\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProduct Contents\u003c\/strong\u003e\u003c\/span\u003e\n\u003cdiv class=\"acontentc\" data-mce-fragment=\"1\"\u003e\n\u003ctable border=\"\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr class=\"firstRow\" data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"156\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eF\u003c\/strong\u003e\u003cstrong data-mce-fragment=\"1\"\u003eA121-01 (25 rxns) \u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\" width=\"145\" data-mce-fragment=\"1\"\u003e\n\u003cstrong data-mce-fragment=\"1\"\u003eFA121-02 (50 rxns)\u003c\/strong\u003e\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eAnnexin V-PE\u003c\/td\u003e\n\u003ctd width=\"156\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e125 μl\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\" width=\"145\" data-mce-fragment=\"1\"\u003e250 μl\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e7-AAD\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e125 μl\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e250 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1×Annexin V Binding Buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e12.5 ml\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e2×12.5 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/div\u003e","brand":"GeneBio Systems","offers":[{"title":"25 Rxns","offer_id":41955111403620,"sku":"FA121-01","price":94.0,"currency_code":"GBP","in_stock":true},{"title":"50 Rxns","offer_id":41955111436388,"sku":"FA121-02","price":174.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/FA121-01.jpg?v=1689870046","url":"https:\/\/www.genebiosystems.com\/en-gb\/products\/transdetect-r-annexin-v-pe-7-aad-cell-apoptosis-detection-kit","provider":"GeneBio ","version":"1.0","type":"link"}