{"product_id":"t7-endonuclease-i","title":"T7 Endonuclease I","description":"\u003cspan class=\"author\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProduct Details\u003c\/strong\u003e\u003c\/span\u003e\n\u003cdiv class=\"acontentc\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eT7 Endonuclease I is a junction-resolving enzyme of 149 amino acid residue, encoded by gene 3 of bacteriophage T7, existing as a stable dimer. Not only does it selectively bind and cleave four-way DNA (Holliday) junctions with high specificity for branched structures in double-stranded DNA, such as cruciform DNA, but also it has a strong preference for cutting single-stranded DNA. It requires metal ions such as magnesium for activity. This product is purified from E. coli expressing the recombinant T7 Endonuclease I (T7EI) gene.\u003cbr data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eDefinition of Activity Unit\u003c\/strong\u003e\u003cbr data-mce-fragment=\"1\"\u003eOne unit is defined as the amount of enzyme required to convert \u0026gt; 90% of 1 μg of supercoiled cruciform pUC(AT) to \u0026gt; 90% linear form in a total reaction volume of 50 μl in 1 hour at 37°C.\u003cbr data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eApplications\u003c\/strong\u003e\u003cbr data-mce-fragment=\"1\"\u003e• Gene mutation and SNP detection for result from TALEN and CRISPR\/CAS9\u003cbr data-mce-fragment=\"1\"\u003e• Recognition and cleavage for non-perfectly matched DNA and Holliday junctions\u003cbr data-mce-fragment=\"1\"\u003e• Randomly cleave single-stranded DNA\u003cbr data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003cbr data-mce-fragment=\"1\"\u003eat -20°C  for one year\u003cbr data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eShipping\u003c\/strong\u003e\u003cbr data-mce-fragment=\"1\"\u003eDry ice (-70 ℃)\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003cdiv class=\"clear\" data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"acontentc\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"clear\" data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cspan class=\"author\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProduct Contents\u003c\/strong\u003e\u003c\/span\u003e\n\u003cdiv class=\"acontentc\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr class=\"firstRow\" data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eLE101-01\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eLE101-02\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eT7 Endonuclease I\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e250 units\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5×250 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e10×T7 Endonuclease I Buffer\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e200 μl\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eT7 Endonuclease I Control Template (40 ng\/μl)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20 μl\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e10×DNA Loading Buffer\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1 ml\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e","brand":"GeneBio Systems","offers":[{"title":"250 units","offer_id":41977668370532,"sku":"LE101-01","price":91.0,"currency_code":"GBP","in_stock":true},{"title":"5×250 units","offer_id":41977668403300,"sku":"LE101-02","price":362.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_3ef51c77-a4c3-4032-9357-5f16b9cb76f5.jpg?v=1691000809","url":"https:\/\/www.genebiosystems.com\/en-gb\/products\/t7-endonuclease-i","provider":"GeneBio ","version":"1.0","type":"link"}