{"product_id":"smad3-ab-204-antibody-cat-csb-pa103091","title":"SMAD3 (Ab-204) Antibody - Cat. #: CSB-PA103091","description":"\u003cp\u003e\u003cb\u003eSize \u003c\/b\u003e:100ul\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone Number\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAliases\u003c\/b\u003e:DKFZP586N0721 antibody; DKFZp686J10186 antibody; hMAD 3 antibody; hMAD-3 antibody; hSMAD3 antibody; HSPC193 antibody; HST17436 antibody; JV15 2 antibody; JV15-2 antibody; JV152 antibody; LDS1C antibody; LDS3 antibody; MAD (mothers against decapentaplegic Drosophila) homolog 3 antibody; MAD homolog 3 antibody; Mad homolog JV15 2 antibody; Mad protein homolog antibody; MAD; mothers against decapentaplegic homolog 3 antibody; Mad3 antibody; MADH 3 antibody; MADH3 antibody; MGC60396 antibody; Mothers against decapentaplegic homolog 3 antibody; Mothers against DPP homolog 3 antibody; SMA and MAD related protein 3 antibody; SMAD 3 antibody; SMAD antibody; SMAD family member 3 antibody; SMAD; mothers against DPP homolog 3 antibody; Smad3 antibody; SMAD3_HUMAN antibody\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Type\u003c\/b\u003e:Polyclonal Antibody\u003c\/p\u003e\u003cp\u003e\u003cb\u003eImmunogen Species\u003c\/b\u003e:Homo sapiens (Human)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniProt ID\u003c\/b\u003e:P84022\u003c\/p\u003e\u003cp\u003e\u003cb\u003eImmunogen\u003c\/b\u003e:Synthesized non-phosphopeptide derived from Human Smad3 around the phosphorylation site of serine 204 (A-G-S(p)-P-N).\n\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRaised in\u003c\/b\u003e:Rabbit\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecies Reactivity\u003c\/b\u003e:Human, Mouse, Rat\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTested Applications\u003c\/b\u003e:ELISA, WB, IHC; WB:1:500-1:3000, IHC:1:50-1:100\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e:Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3\/SMAD4 complex, activates transcription. Also can form a SMAD3\/SMAD4\/JUN\/FOS complex at the AP-1\/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.\u003cbr\u003e\u003cbr\u003eZhang Y., Nature 383:168-172(1996).\u003cbr\u003eRiggins G.J., Nat. Genet. 13:347-349(1996).\u003cbr\u003eArai T., Cancer Lett. 122:157-163(1998).\u003cbr\u003e\n\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality\u003c\/b\u003e:Polyclonal\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e:IgG\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurification Method\u003c\/b\u003e:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugate\u003c\/b\u003e:Non-conjugated\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBuffer\u003c\/b\u003e:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eForm\u003c\/b\u003e:liquid\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStroage\u003c\/b\u003e:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Names\u003c\/b\u003e:SMAD3\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e:Epigenetics and Nuclear Signaling;Cancer;Metabolism;Signal transduction?Stem cells\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":40483787538532,"sku":"CSB-PA103091","price":510.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/no_image_default_image-jpeg_542c2459-5fec-48be-bacc-ce6f989a5abd.jpg?v=1660076255","url":"https:\/\/www.genebiosystems.com\/en-gb\/products\/smad3-ab-204-antibody-cat-csb-pa103091","provider":"GeneBio ","version":"1.0","type":"link"}