{"product_id":"simian-tracp-5b-tartsimiane-resistant-acid-phosphatase-5b-elisa-kit","title":"Simian TRACP-5b(TartSimiane Resistant Acid Phosphatase 5b) ELISA Kit","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:96T\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e:Simian\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAlternative Names\u003c\/b\u003e:ACP5;TRAP5b;SPENCDI\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay Type\u003c\/b\u003e:Sandwich\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSensitivity\u003c\/b\u003e:0.45 ng\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStandard\u003c\/b\u003e:50 ng\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDetection range\u003c\/b\u003e:0.78-50 ng\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSample type\u003c\/b\u003e:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay length\u003c\/b\u003e:3.5h\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Area\u003c\/b\u003e:Signal Transduction;Signal Transduction;Metabolism\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTest principle\u003c\/b\u003e:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian TRACP-5b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian TRACP-5b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian TRACP-5b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian TRACP-5b in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniProt ID\u003c\/b\u003e:\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48644992663652,"sku":"ELK11611","price":566.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_d179167d-83c6-4430-8712-2f674f9e14e8.jpg?v=1782663195","url":"https:\/\/www.genebiosystems.com\/en-gb\/products\/simian-tracp-5b-tartsimiane-resistant-acid-phosphatase-5b-elisa-kit","provider":"GeneBio ","version":"1.0","type":"link"}