{"product_id":"recombinant-mouse-pla2g2e-protein-his-tag","title":"Recombinant Mouse PLA2G2E Protein (His Tag)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 100μg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShipping\u003c\/b\u003e: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExp date\u003c\/b\u003e: 12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_II\u003c\/b\u003e: Recombinant Proteins\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_III\u003c\/b\u003e: Others\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Synonym\u003c\/b\u003e: PLA2G2E\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e: Mouse\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExpression Host\u003c\/b\u003e: HEK293 Cells\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eFusion tag\u003c\/b\u003e: C-His\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUNIProt ID\u003c\/b\u003e: Q9QUL3\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e: Q9QUL3\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: Group IIE secretory  phospholipase A2, also known as GIIE sPLA2, sPLA2-IIE, Phosphatidylcholine  2-acylhydrolase 2E and PLA2G2E is a secreted protein which belongs to  thephospholipase A2 family. Mammalian secretory phospholipase A2s (  sPLA2s ) form a family of structurally related enzymes that are involved in a  variety of physiological and pathological processes via the release of  arachidonic acid from membrane phospholipids or the binding to specific  membrane receptors. Phospholipases A2 \/ PLA2 are enzymes that release fatty  acids from the second carbon group of glycerol. This particular phospholipase  specifically recognizes the sn-2 acyl bond of phospholipids and catalytically  hydrolyzes the bond releasing arachidonic acid and lysophospholipids.  Phospholipases A2 \/ PLA2 are commonly found in mammalian tissues as well as  insect and snake venom. Venom from both snakes and insects is largely composed  of melittin, which is a stimulant of Phospholipases A2 \/ PLA2. Due to the  increased presence and activity of Phospholipases A2 \/ PLA2 resulting from a  snake or insect bite, arachidonic acid is released from the phospholipid  membrane disproportionately. As a result, inflammation and pain occur at the  site. PLA2G2E catalyzes the calcium-dependent hydrolysis of the 2-acyl groups  in 3-sn-phosphoglycerides. Has a preference for arachidonic-containing  phospholipids.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eActivity\u003c\/b\u003e: Not validated for activity\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e: Met 1-Cys 142\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:  \u0026gt; 97 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Lyophilized from sterile PBS, 10% glycerol, pH 7.4\u003cbr\u003eNormally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.\u003cbr\u003ePlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e: Please refer to the printed manual for detailed information.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:  \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated MW\u003c\/b\u003e: 15.5 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObservedMW\u003c\/b\u003e: 18 kDa\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":47986029035620,"sku":"PKSM040548","price":809.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_740c0136-1327-453a-8e00-34ca9b3c8c50.jpg?v=1772480254","url":"https:\/\/www.genebiosystems.com\/en-gb\/products\/recombinant-mouse-pla2g2e-protein-his-tag","provider":"GeneBio ","version":"1.0","type":"link"}