{"product_id":"recombinant-human-vascular-endothelial-growth-factor-a-proteinvegfa-active","title":"Recombinant Human Vascular endothelial growth factor A protein(VEGFA) (Active)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:100ug. Other sizes are also available. Please contact us. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e:Cancer\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniprot NO.\u003c\/b\u003e:P15692\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniprot Entry Name\u003c\/b\u003e:VEGFA_HUMAN\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene Names\u003c\/b\u003e:VEGFA,VEGF\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecies\u003c\/b\u003e:Homo sapiens (Human)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSource\u003c\/b\u003e:E.Coli\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExpression Region\u003c\/b\u003e:27-191aa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e:M+APMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQENPCGP CSERRKHLFV QDPQTCKCSC KNTDSRCKAR QLELNERTCR CDKPRR\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProtein Description\u003c\/b\u003e:Partial of Isoform VEGF165\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTag Info\u003c\/b\u003e:Tag-Free\u003c\/p\u003e\u003cp\u003e\u003cb\u003eMol. Weight\u003c\/b\u003e:19.3 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBiological_Activity\u003c\/b\u003e:Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is between 1.0-8.0 ng\/ml.\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:\u0026gt;95% as determined by SDS-PAGE and HPLC.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:Less than 1.0 EU\/µg as determined by LAL method.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eForm\u003c\/b\u003e:Lyophilized powder\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBuffer\u003c\/b\u003e:Lyophilized from a  0.2 µm filtered  PBS, pH 7.4\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg\/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?\/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eNotes\u003c\/b\u003e:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAlternative Name\/ Alias\u003c\/b\u003e:VEGF-A, VPF\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRelevance\u003c\/b\u003e:Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1\/VEGFR1 and KDR\/VEGFR2 receptors, heparan sulfate and heparin. NRP1\/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. {ECO:0000269|PubMed:11427521, ECO:0000269|PubMed:16489009}.\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePubMed ID\u003c\/b\u003e:2479986; 2479987; 1711045; 1791831; 1567395; 9054410; 9878851; 9450968; 10464055; 12124351; 19054851; 14574404; 15489334; 7678805; 15340161; 2584205; 10067980; 11563986; 11427521; 11731620; 11978667; 15520188; 14764596; 15896327; 16489009; 22009797; 9207067; 9336848; 9351807; 9922142; 9634701\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFunction\u003c\/b\u003e:Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1\/VEGFR1 and KDR\/VEGFR2 receptors, heparan sulfate and heparin. NRP1\/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). \u003c\/p\u003e\u003cp\u003e\u003cb\u003eInvolvement in disease\u003c\/b\u003e:Microvascular complications of diabetes 1 (MVCD1) \u003c\/p\u003e\u003cp\u003e\u003cb\u003eSubcellular Location\u003c\/b\u003e:Secreted\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProtein Families\u003c\/b\u003e:PDGF\/VEGF growth factor family\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTissue Specificity\u003c\/b\u003e:Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. A higher level expression seen in pituitary tumors as compared to the pituitary gland. \u003c\/p\u003e\u003cp\u003e\u003cb\u003ePaythway\u003c\/b\u003e:HIF-1signalingpathway\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHGNC Database Link\u003c\/b\u003e:https:\/\/www.genenames.org\/cgi-bin\/gene_symbol_report?hgnc_id=HGNC:12680\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniGene Database Link\u003c\/b\u003e:https:\/\/www.ncbi.nlm.nih.gov\/UniGene\/clust.cgi?ORG=Hs\u0026amp;CID=73793\u003c\/p\u003e\u003cp\u003e\u003cb\u003eKEGG Database Link\u003c\/b\u003e:https:\/\/www.genome.jp\/dbget-bin\/www_bget?hsa:7422\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSTRING Database Link\u003c\/b\u003e:https:\/\/string-db.org\/network\/9606.ENSP00000361125\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOMIM Database Link\u003c\/b\u003e:https:\/\/www.omim.org\/entry\/192240192240192240\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":40529899257956,"sku":"CSB-AP002601HU","price":1221.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/no_image_default_image-jpeg_558f2d64-27c5-450e-8512-8453fd6402d7.jpg?v=1661105816","url":"https:\/\/www.genebiosystems.com\/en-gb\/products\/recombinant-human-vascular-endothelial-growth-factor-a-proteinvegfa-active","provider":"GeneBio ","version":"1.0","type":"link"}