{"product_id":"recombinant-human-srpk1-protein-his-gst-tag","title":"Recombinant Human SRPK1 Protein (His \u0026 GST Tag)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 50μg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShipping\u003c\/b\u003e: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExp date\u003c\/b\u003e: 12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_II\u003c\/b\u003e: Recombinant Proteins\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_III\u003c\/b\u003e: Others\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Synonym\u003c\/b\u003e: RP3-422H11.1;SFRSK1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e: Epigenetics and Nuclear Signaling\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e: Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExpression Host\u003c\/b\u003e: Baculovirus-Insect Cells\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eFusion tag\u003c\/b\u003e: N-His-GST\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUNIProt ID\u003c\/b\u003e: Q96SB4\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e: AAH38292.1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: Serine  \/ threonine-protein kinase SRPK1, also known as SFRS protein kinase 1,  Serine\/arginine-rich protein-specific kinase 1, SR-protein-specific kinase 1  and SRPK1, is a cytoplasm and nucleus protein which belongs to theprotein  kinase superfamily and CMGC Ser\/Thr protein kinase family. Isoform 2 of  SRPK1 is predominantly expressed in the testis but is also present at lower  levels in heart, ovary, small intestine, liver, kidney, pancreas and skeletal  muscle. Isoform 1 of SRPK1 is only seen in the testis, at lower levels than  isoform 2. SRPK1 hyperphosphorylates RS domain-containing proteins such as  SFRS1, SFRS2 and ZRSR2 on serine residues during metaphase but at lower levels  during interphase. SRPK1 plays a central role in the regulatory network for  splicing, controlling the intranuclear distribution of splicing factors in  interphase cells and the reorganization of nuclear speckles during mitosis.  SRPK1 locks onto SFRS1 to form a stable complex and processively phosphorylates  the RS domain. SRPK1 appears to mediate HBV core protein phosphorylation which  is a prerequisite for pregenomic RNA encapsidation into viral capsids.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eActivity\u003c\/b\u003e: Not validated for activity\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e: Glu 2-Ser 655\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:  \u0026gt; 80 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% Glycerol, 0.1mMEDTA, 0.5mM PMSF, 1mM TCEP, pH7.5.\u003cbr\u003eNormally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.\u003cbr\u003ePlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e: Please refer to the printed manual for detailed information.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:  \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated MW\u003c\/b\u003e: 102 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObservedMW\u003c\/b\u003e: 120 kDa\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":47972854104164,"sku":"PKSH030864","price":466.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_503acf8d-033e-46d8-87b9-46b5ffc69b4d.jpg?v=1772200633","url":"https:\/\/www.genebiosystems.com\/en-gb\/products\/recombinant-human-srpk1-protein-his-gst-tag","provider":"GeneBio ","version":"1.0","type":"link"}